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In order to study the effects of different storage temperatures and preservatives on the preservation effects of Qiuyang pear fruits, the effects of 0.5 μL/L 1 -methylcyclopropene (1 -MCP) fumigation for 12 h and ethylene adsorbent treatments on the storage qualities of Qiuyang pears were investigated under different storage temperatures (-1 °C and 0 °C). The results showed that after 120 days of storage, the decay rates of fruits stored at 0°C was lower than that stored at -1°C under the same treatment conditions. At 270 days, the decay rates of fruits stored at 0 °C and -1°C in the two preservatives treatment groups were lower than those in CK group. The fruits peels brightness of CK group and ethylene sorbent treatment group at 0°C was higher than that stored at -1 °C. After 240 days of storage, the fruits hardness of 1-MCP treatment group was remarkably higher than that of other treatments groups (P<0.05), and the fruits hardness of 1-MCP treatment group stored at 0°C was significantly higher than that stored at -1°C at 270 days (P<0.05). The titratable acid contents of fruits in the two preservatives treatment groups were higher than that in CK group at 270 days of storage. The soluble solids contents of fruits in each group presented no significant changes during storage. In conclusion, Qiuyang pears presented low decay rates and well maintained peels brightness when stored at 0°C. The two preservatives treatments could both reduce the decay rates and delay the decline of titratable acid contents of Qiuyang pears, and the 1-MCP treatment showed better effects that could effectively maintain peel brightness and fruit firmness. [ABSTRACT FROM AUTHOR]

Objective: To study the effects of NS398, a selective cyclooxygenase-2 (COX-2) inhibitor, on the transcription and translation of BCL-3 and its regulatory gene cyclin D1 in colon cancer cell line SW480., Methods: Human colon cancer cells SW480 were divided into two groups: SW480 cells in experimental group were treated with NS398 in different concentrations(25 micromol/L, 50 micromol/L, 100 micromol/L and 200 micromol/L) for 48 h or 72 h. SW480 cells in control group were treated with media which did not contain NS398. Then the expressions of BCL-3 and cyclin D1 were detected by RT-PCR, Western blot, and immunocytochemistry., Results: At 48 hours RT-PCR showed that BCL-3 mRNA and cyclin D1 mRNA decreased in a dose-dependent manner in the experimental group. However, there were no significant differences in the levels of BCL-3 protein and cyclin D1 protein between two groups (P>0.05). At 72 hours, BCL-3 protein and cyclin D1 protein also decreased in a dose-dependent manner in the experimental group. When the concentration of NS398 reached 100 micromol/L, the differences between the two groups in the expression of BCL-3 mRNA and protein became statistically significant (P<0.01). When the concentration of NS398 reached 50 micromol/L, the differences in the expression of cyclin D1 mRNA and protein were statistically significant (P<0.05)., Conclusions: BCL-3 is expressed in colon cancer cell line SW480. COX-2 inhibitor can inhibit the expression of BCL-3 and cyclin D1 in a dose-dependent manner. NS398 may down-regulate the expression of cyclin D1 through BCL-3.

In this paper, the net photosynthetic rate (Pn), actual photochemical efficiency of PS II (PhiPSII), photochemical quenching (qp), and maximal photochemical efficiency of PS II in light (Fv'/Fm') of apricot (Prunus armeniaca) leaves in solar greenhouse were measured, and the effects of low temperature (7 degrees C) and weak light (200 micromol x m(-2) x s(-1) PFD) on the photoinhibition of PS I and PS II were investigated. The results showed that the optimal temperature for the photosynthesis of apricot leaves was around 25 degrees C, and the photosynthetic capacity was reduced greatly by the low temperature and weak light, inducing a markedly increased excitation press (1-qp) and in turn, resulting in photoinhibition. The functions of both PS I and PS II were damaged by the low temperature and weak light. Comparing with those only subjected to low temperature, the leaves subjected to both low temperature and weak light had a decreased activity of PS I, with a decrement of 28.26% within 2 h, but their maximal photochemical effeciency of PS II (Fv/Fm) had little change in the same period, suggesting that under low temperature and weak light, PS I was more suffered from photoinhibition than PS II.