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Objective To evaluate and compare the impact of three root canal sealers (C-Root SP, iRoot SP, and AH-Plus Jet) on the sealing performance in single-cone root canal filling using micro-computed tomography (Micro-CT). Methods Ninety mandibular single-rooted extracted teeth meeting inclusion criteria were collected, prepared with Protaper Next up to X3, and randomly assigned to three experimental groups which were C-Root SP group (Group A), iRoot SP group (Group B), and AH-Plus Jet group (Group C). Subsequently, all three groups underwent single-cone root canal filling, followed by one week of oral environment simulation. The sealing performance of the three root canal sealers was evaluated by measuring the proportion of the root canal volume and its rate using Micro-CT. Results In different segments of the roots (upper 1/3, middle, and lower), identifiable bubbles were observed in the Group A, Group B and Group C, with volumes of (0.15±0.03)mm3, (0.22±0.11)mm3, and (0.34±0.28) mm3, respectively. Overall, Group A exhibited the smallest bubble volume (0.15±0.03) mm3. Group A and B were similar, they were superior to group C. (P

Objective To establish a new threshold of prostate specific antigen (PSA) for early warning of prostate cancer (PCa) among people with type 2 diabetes mellitus (T2DM). Methods We retrospectively analyzed the clinical data of 2 151 patients who underwent prostate biopsy for the first time admitted to Shanghai Tenth People's Hospital from January 2012 to December 2018. Taking the positive pathological results of prostate biopsy as the gold standard, all patients were divided into T2DM group and non-T2DM group for comparison. Among them, patients with PCa were divided into PCa-T2DM group and PCa-non-T2DM group. Using the method of big data analysis and machine learning, a new PSA threshold for early warning of PCa in the T2DM population (the original threshold 4.0 ng/ml) was obtained, and its sensitivity and specificity were calculated. Probability function fitting was used to estimate the distribution of PSA levels in the overall population, support vector machine was used to calculate the new threshold, and receiver operating characteristic (ROC) curve was used to test its diagnostic efficacy. Results The detection rate of PCa in 2 151 patients was 35.89% (772/2 151). All subjects were divided into T2DM group (496 cases, 23.06%) and non-T2DM group (1 655 cases, 76.94%). The PSA of the T2DM group was lower than that of the non-T2DM group, and the difference was statistically significant (P<0.01). The new PSA threshold for PCa screening for T2DM population was 3.27 ng/ml. In the T2DM group, with 3.27 ng/ml of PSA as the diagnostic cut-off point, the area under the ROC curve was 0.83, and the diagnostic sensitivity and specificity were 94.53% and 41.36%, respectively. In the overall population, with 4 ng/ml of PSA as the diagnostic cut-off point, the area under the ROC curve was 0.83, and the sensitivity and specificity were 91.54% and 46.78%, respectively. There was no statistically significant difference between the two groups (P>0.05). Conclusions The serum PSA levels in T2DM patients reduced. The new PSA threshold (3.27 ng/ml) for type 2 diabetes mellitus has a better early warning effect on prostate cancer, which is conducive to improving the early diagnosis rate of prostate cancer in T2DM patients. [ABSTRACT FROM AUTHOR]

Objective: To study the effect of light intensity on physiological and biochemical characteristics of Chrysanthemum morifolium at the vegetative stage., Method: The dynamic response of physiological and biochemical indexes of Ch. morifolium were measured under different treatments (100%, 80%, 60%, 40% and 20% of the full sunlight) at the vegetative stage., Result: The physiological and biochemical indexes of Ch. morifolium showed dynamic changes with the progress of growth and the increase of the treatment time. The soluble sugar content decreased when the light intensity reduced, and had a significant positive correlation with the light intensity. Soluble protein content rose firstly and fell later, malondialdehyde content increased, superoxide dismutase and catalase activity decreased initially and increased afterwards., Conclusion: Proper shading benefits the nitrogen accumulation of Ch. morifolium at the vegetative stage, and reduces the strength of stress condition. The suitable light environment for growth of Ch. morifolium at the vegetative stage is about 80%-60% of full sunlight and the optimum treatment time is 20-40 days.

Objective: To study the effects of soil factors on the active component content of Chrysanthemum morifolium and screen out the leading factors., Method: The active component of water soluble extracts, flavonoids, phenolic compounds and mineral elements were determined and chemical properties and mineral elements of soil were analyzed for studying the effects on Ch. morifolium through correlation, stepwise regression, path and grey correlation analysis., Result: Soil available P and K were the most important factors that affected the active component content of Ch. morifolium, followed by urease, phosphatase and invertase activities and organic matter. The mineral elements in Ch. morifolium and in soil correlated well, P and K were enriched in the plant mostly, followed by Cd, Ca, Zn, Cu. The main leading factors of mineral elements in soil were P and K, followed by Fe, Cu and Zn., Conclusion: Soil was one of the important factors which affected the active component content of Ch. morifolium.

Objective: To develop a RP-HPLC method for the determination of quercetin, luteolin, apigenin and acacetin in Flos Chrysanthemi indici., Method: An Eclipse XDB-C18 column (4.6 mm x 250 mm, 5 microm) was used at 25 degrees C with the mobile phases of methanol-0.2% phosphatic acid in a gradient manner. The flow rate was set at 1.0 mL x min(-1). The detection wavelength was 350 nm., Result: The linear response ranged from 1.02-20.48 mg x L(-1) for quercetin (r = 0.9994, n = 5), 1.03-20.54 mg x L(-1) for luteolin (r = 0.9992, n = 5), 1.12-22.40 mg x L(-1) for apigenin (r = 0.9995, n = 5), 1.01-20.22 mg x L(-1) for acacetin (r = 0.9998, n = 5), respectively. Recoveries were 101.3% with RSD 1.3% for quercetin, 100.62% with RSD 1.4% for luteolin, 98.42% with RSD 1.7% for apigenin and 99.02% with RSD 0.8% for acacetin. A significant difference (alpha = 0.01) among the contents of four flavonoids and total flavonoids was found., Conclusion: The method is quick, simple and repeatable for simultaneous determination of quercetin, luteolin, apigenin and acacetin in Flos Chrysanthemi Indici.

Objective: To investigate the effect of light intensity on growth and photosynthetic of Chrysanthemum morifolium., Method: The growth characteristics of C. morifolium were measured under different treatments (100%, 80%, 60%, 40% and 20% of full sunlight). The photosynthetic characteristics and chlorophyll fluorescence parameters of leaves under different light intensity were determined by a LI-6400 photosynthesis system and a PAM-2100 chlorophyll fluorescence system., Result: With the reduction of irradiance, the diameter of the stem reduced, plant height, leaf length, leaf width and length/width raised, assimilation product increased; Content of photosynthetic pigment increased between light intensity 100%-40% reduced under 20% treatment, chlorophyll a/b decreased. Light compensation point (LCP), apparent quantum yield (AQY) increased first and reduced later, photosynthesis rate (P(n)), stomatal conductance (G(s)), intercellular CO2 concentration (C(i)) and transpiration rate (T(r)) decreased, stomatal limitation value (L(s)) rose. Chlorophyll fluorescence parameters F(v)/F(m) increased, phiPS II, F(v)'/F(m)', ETR and qP increased between irradiance 100%-60%, NPQ decreased, such as phiPS II decreased and NPQ increased when irradiance was lower than 40%., Conclusion: Weak light condition was unfavorable to the growth of C. morifolium and the light conditions of culture should be control between 80%-60% of full sunlight.

Objective: To study seed quality test, and establish seed quality classification standard of Prunella vulgaris., Method: The seed quality and planting quality of P. vulgaris from different population were measured and these indexes were analyzed by K-clustering., Result and Conclusion: The seed purity, 1000 seeds weight, seed moisture content, seed viability of the seed of P. vulgaris from different population were measured, and the seed quality classification standard of P. vulgaris.