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The keratin solution was prepared with TCEP. The role of temperature and SDS on dissolution time and dissolution rate in the process of extraction was studied. The temperature had a great influence on the dissolution time and dissolution rate. SDS can accelerate the dissolution rate of woof, but had little impact on the final dissolubility. When the temperature was low (<70°C), SDS had a great influence on the final dissolubility; when the temperature was high (>70°C), SDS had little influence on the final dissolubility. The amounts of SDS had little influence on dissolution time, with the increased of SDS. There was no significant increase for solubilization. Through microscope, the dissolution of wool was a process of swelling. [ABSTRACT FROM AUTHOR]

In this paper, the structure, the molecular weight and dissolution method of wool were introduced. Reduction was the main method of dissolving wool, only to cut off the disulfide bonds intact keratin primary structure was the main direction of hydrolysis of keratin. Some problems were discussed appear in the reduction dissolution of the the wool and put forward some opinions and ideas. Some new dissolved agent and crosslinking methods of protein were introduced. In view of the molecular weight of keratin was small, to increase the molecular weight of keratin solution by adding cross-linking agent in the keratin solution was the main method to improve the application performance. [ABSTRACT FROM AUTHOR]

Keratin is a natural protein which has a good biocompatibility, non-toxic and skin-friendly properties , it contains large amounts of amino and hydroxyl groups. These features make the keratin show a good prospects in the textile, biomedical, cosmetic and engineering applications. This paper reports the present situation of extraction of keratin from wool and application of keratin solution status. Reduction is the main method of dissolving wool, only to cut off the disulfide bonds intact keratin primary structure is the main direction of hydrolysis of keratin, but the keratin solution is instability. Major applications are fabric finishing, spinning and film-forming at present, there are still many problems waiting for us to solve. The prospect of application of the keratin is good ,but we have to meet many challenges. [ABSTRACT FROM AUTHOR]

Objective: To investigate the nutritional risk in children with severe pneumonia using the Screening Tool for the Assessment of Malnutrition in Paediatrics (STAMP) and the association between nutritional risk and adverse clinical outcomes., Methods: According to the STAMP score, 216 children with severe pneumonia were classified into high nutritional risk group (HR group; n=98), moderate nutritional risk group (MR group; n=65), and low nutritional risk group (LR group; n=53). Fasting blood samples were collected to measure the levels of insulin-like growth factor-1 (IGF-1), adiponectin, leptin, non-esterified fatty acid (NEFA), albumin, transferrin, prealbumin, and retinol binding protein (RBP). The adverse clinical outcomes were recorded., Results: Compared with the MR and LR groups, the HR group had significantly lower serum levels of IGF-1, leptin, adiponectin, prealbumin, and RBP, as well as a significantly higher serum level of NEFA (P<0.05). Compared with the MR and LR groups, the HR group had a significantly higher proportion of children admitted to the intensive care unit and a significantly longer duration of mechanical ventilation (P<0.05). The HR group had a significantly longer mean hospital stay and a significantly higher incidence rate of complications compared with the LR and MR groups (P<0.05)., Conclusions: Nutritional risk screening has an important value in evaluating the clinical outcome of children with severe pneumonia, and children at a higher nutritional risk tend to have more adverse clinical outcomes.