27 results on '"Wang, Dong-Dong"'
Search Results
2. Analysis of risk factors for transformation of traumatic subdural effusion into chronic subdural hematoma.
- Author
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YI Pan, WANG Cun-zu, CHEN Pin, WANG Dong-dong, WANG Hui, HE Shi-wei, and ZENG Wei
- Subjects
CHRONIC disease risk factors ,BRAIN injuries ,COMPUTED tomography ,CONFIDENCE intervals ,MAGNETIC resonance imaging ,MENINGITIS ,RISK assessment ,STATISTICS ,SUBDURAL hematoma ,LOGISTIC regression analysis ,DESCRIPTIVE statistics ,ODDS ratio ,DISEASE complications - Abstract
Objective To analyze the risk factors of traumatic subdural effusion (TSE) transforming into chronic subdural hematoma (CSDH). Methods A total of 75 patients with TSE after traumatic brain injury (TBI) were included from December 2017 to July 2019. CT or MRI reexamination during treatment and follow-up were performed to observe whether CSDH was transformed and relevant influencing factors were analyzed. Univariate and multivariate Logistic regression analyses were performed to screen the risk factors for the transformation of TSE into CSDH. Results Logistic regression analysis showed that the higher effusion density (OR = 6.021, 95% CI: 1.047-34.631; P = 0.044), subdural effusion increases for a long time (OR = 1.253, 95% CI: 1.002-1.568; P = 0.048), the thicker effusion (OR = 2.080, 95% CI: 1.267-3.414; P = 0.004) and brain atrophy (OR = 35.392, 95% CI: 1.397-896.474; P = 0.031) were risk factors for the transformation of TSE into CSDH. Conclusions The subdural effusion increases for a long time, higher effusion density, thicker effusion and brain atrophy are the risk factors associated with the transformation of TSE into CSDH. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
3. Quality standard of uygur medicine Medicago sativa seeds.
- Author
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DING Wen-huan, XU Hai-yan, WANG Dong-dong, LI Jie, and TIAN Shu-ge
- Published
- 2013
- Full Text
- View/download PDF
4. Research on Demand Expressing Mechanisms of Rural Sports Public Goods.
- Author
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TAN Zhi-wu, CAO Qing-rong, WANG Dong-dong, and LI Ze-qun
- Published
- 2011
5. A Review of the Researches on the Rural Sports Public Goods Supply in China.
- Author
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ZHONG Wu and WANG Dong-dong
- Published
- 2013
6. Model for coordinated development evaluation of sports and social economy in rural areas based on SPA.
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Zeng Zhi-jian and Wang Dong-dong
- Published
- 2011
7. Research on the Situation and Sustainable Development of Sunny Sports.
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Wang Yah and Wang Dong-dong
- Published
- 2011
8. Behavioral Rules and Regulations for the Supply Subject of Public Products for Rural Sports Viewed from the Game Theory.
- Author
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Wang Dong - dong
- Published
- 2009
9. Sequence-paleogeography and coal accumulation of the Carboniferous-Permian in the North China Basin.
- Author
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SHAO Long-yi, DONG Da-xiao, LI Ming-pei, WANG Hai-sheng, WANG Dong-dong, LU Jing, ZHENG Ming-quan, and CHENG Ai-guo
- Abstract
The authors studied the depositional environments, sequence stratigraphy, paleogeography and coal accumulation of the Carboniferous-Permian paralic coal measures based on data from outcrop and borehole sections. The sequence boundaries were represented by regional disconformity, transgression direction switching surface, the base of incised valley fill sandstone, facies-reversal surface and associated paleosols. According to these sequence boundaries, the coal measures were subdivided into 7 third-order sequences and related systems tracts. For the paleogeography analysis, the contours of a variety of lithofacies parameters were drawn, including strata thickness, thickness ratio of sandstone and mudstone, limestone contents, and coal thickness. Based on these contours, the lithofacies paleogeography maps of each third-order sequence were reconstructed. The comparison of the contours of coal thickness with the distribution of facies units of the lithofacies paleogeography was made, and it revealed that the favorable coal-accumulating environments were deltas, fluvial plain and lagoon-tidal flat. Coal accumulation was strong in SQ2 (middle and upper Taiyuan Formation) and SQ3 (Shanxi Formation) with the coal accumulating centers were distributed in the Zhungeer, Datong, Shuozhou, Wuhai, Fengfeng areas, and the Beijing-Tainjin-Tangshan area. Coal accumulation in SQ4 (Xiashihezi Formation) and SQ5 (first member of the Shangshihezi Formation) was weak and the coal-accumulating centers were distributed in the southern part of the North China Basin, including Huainan and Huaibei coal fields. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
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10. [Acupuncture for chronic prostatitis/chronic pelvic pain syndrome: a randomized controlled trial].
- Author
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Li B, Wang DD, Qiao XD, Yuan AH, Jiang P, Xu JP, Yang J, and Zhang QP
- Subjects
- United States, Male, Humans, Quality of Life, Punctures, Prostatitis therapy, Acupuncture Therapy, Meridians
- Abstract
Objective: To observe the short-term efficacy, long-term efficacy and safety of acupuncture for chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS)., Methods: Forty-two patients with CP/CPPS were randomly divided into an acupuncture group (21 cases, 1 case dropped off) and a sham acupuncture group (21 cases). The patients in the acupuncture group were treated with acupuncture at bilateral Zhongliao (BL 33), Huiyang (BL 35), Shenshu (BL 23) and Sanyinjiao (SP 6); the needling depth of Zhongliao (BL 33) and Huiyang (BL 35) was 60 to 80 mm, while Shenshu (BL 23) and Sanyinjiao (SP 6) was directly punctured of 30 mm. The patients in the sham acupuncture group were treated with acupuncture at non-acupoints, including points 2 cm next to Shenshu (BL 23), Zhongliao (BL 33) and Huiyang (BL 35), and the midpoint of the connecting line between the spleen meridian and the kidney meridian. All the non-acupoints were treated with directly puncture of 2 to 3 mm. The needles were left for 30 min in both groups, once every other day in the first four weeks, three times a week, and twice a week in the next four weeks, totally 20 treatments. Before treatment, after treatment and in follow-up of 24 weeks after treatment completion, the National Institutes of Health-chronic prostatitis symptom index (NIH-CPSI) score and urinary flow rate were observed in both groups; the clinical efficacy and safety were evaluated., Results: Compared with those before treatment, the pain and discomfort scores, urination symptoms scores, quality of life scores and total scores of NIH-CPSI in both groups were reduced after treatment in the two groups ( P <0.01), while each item score and total score of NIH-CPSI in the acupuncture group were reduced in follow-up ( P <0.01, P <0.05). After treatment and in follow-up, each item score and total score of NIH-CPSI in the acupuncture group were lower than those in the sham acupuncture group ( P <0.05, P <0.01). After treatment, the maximum and average urinary flow rates in the acupuncture group were higher than those before treatment ( P <0.05), and the average urinary flow rate in the acupuncture group was higher than that in the sham acupuncture group ( P <0.05). The total effective rate was 75.0% (15/20) in the acupuncture group, which was higher than 42.9% (9/21) in the sham acupuncture group ( P <0.05). No significant adverse reactions were observed in the two groups, and there was no significant difference in the incidence of adverse reactions between the two groups ( P >0.05)., Conclusion: Acupuncture could effectively alleviate the clinical symptoms, improve quality of life, and has a sustained, safe and reliable therapeutic effect in patients with CP/CPPS.
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- 2023
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11. [Temporal and Spatial Variation Characteristics and Source Analysis of Agricultural Non-point Source Pollution Load in Guangdong During the Past 20 Years].
- Author
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Ge XJ, Huang B, Yuan ZJ, Wang DD, Wang QQ, Chen JC, and Xie ZY
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- Animals, Biological Oxygen Demand Analysis, China, Environmental Monitoring methods, Fertilizers analysis, Humans, Livestock, Nitrogen analysis, Phosphorus analysis, Poultry, Non-Point Source Pollution analysis, Water Pollutants, Chemical analysis
- Abstract
The agricultural non-point source pollution (ANPSP) load in Guangdong province is very large and has a serious impact on the regional ecological environment. Inventory analysis was used to assess and analyze the temporal and spatial variation characteristics of the ANPSP load of Guangdong province during 1999-2019, and the sources of ANPSP were discussed, as well as the relationship between them. The results showed that, during the past 20 years, the total ANPSP pollution loads of Guangdong province decreased by 6.08%, and the pollution loads of chemical oxygen demand (COD), total nitrogen (TN), and total phosphorus (TP) increased by -11.88%, 4.99%, and 26.17%, respectively. The input intensity of chemical fertilizers and pesticides increased by 112.19% and 60.38%, respectively. The Pearl River Delta had the highest ANPSP loads in Guangdong province, followed by those in northern, western, and eastern Guangdong. Livestock and poultry breeding were the main sources of COD, the total percent fertilizers and livestock and poultry breeding were the main sources of TN, and livestock and poultry breeding and aquaculture were the main sources of TP. In addition, the contribution of pollutants discharged from aquaculture showed an obvious increasing trend. There were certain differences in the pollution sources in different regions. In western Guangdong, northern Guangdong, and eastern Guangdong, livestock and poultry breeding were the main sources of COD and TP, and fertilizer was the main source of TN; by contrast in the Pearl River Delta, aquaculture had become the main source of TN and TP pollution loads. The correlation results showed that the decline in the total ANPSP in Guangdong province was mainly due to the increase in high urbanization rate and the decrease in the proportion of rural population. In general, there were stage changes in the time and differences in spatial characteristics and sources of ANPSP in Guangdong province. A combination of comprehensive treatment and targeted pollution treatment should be adopted, and fertilizer reduction measures and pollution treatment in the aquaculture should be strengthened in an all-around way, focusing on strengthening the treatment of pollution from aquaculture in the Pearl River Delta region and the treatment of rural life pollution in northern Guangdong.
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- 2022
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12. [Professor ZHANG Qing-ping 's clinical experience of acupuncture for peripheral facial paralysis].
- Author
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Wang DD, Li B, Zha YM, Zou H, Yao TT, Gu W, Yang J, and Zhang QP
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- Acupuncture Points, Humans, Acupuncture, Acupuncture Therapy, Facial Paralysis therapy, Meridians, Moxibustion
- Abstract
Professor ZHANG Qing - ping 's clinical experience and characteristics of acupuncture for peripheral facial paralysis were introduced from three aspects: differentiation of meridians and tendons, treatment by stages, and examples of proven cases. In the treatment of peripheral facial paralysis, Professor ZHANG carefully examines the situation of pathogenic factors and health. In the acute stage, the treatment focuses on eliminating pathogenic factors and supporting the health; in the recovery stage, penetrating needling is recommended with strengthening the health and eliminating pathogenic factors as main purpose; in the sequelae stage, wheat-grain moxibustion is recommended with strengthening the health as main purpose. This treatment plan shows superior clinical efficacy.
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- 2021
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13. [Clinical application evaluation and revision suggestions of clinical practice guideline on traditional Chinese medicine therapy alone or combined with antibiotics for sepsis].
- Author
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Zhang R, Chen YS, Zhao GZ, Wang DD, Ha YX, Huang P, Hu J, Feng S, Guo YH, Liao X, Xie YM, Zhang JH, Zhang BL, Li B, and Liu QQ
- Subjects
- China, Humans, Surveys and Questionnaires, Anti-Bacterial Agents therapeutic use, Medicine, Chinese Traditional, Sepsis drug therapy
- Abstract
To investigate the clinical application of clinical practice guideline on traditional Chinese medicine therapy alone or combined with antibiotics for sepsis, in order to promote the follow-up revision and further promotion of the Guidelines. Copies of 500 application evaluation questionnaire and 500 copies of applicability evaluation questionnaire were given to the clinicians who had used this Guideline in China, both in a form of registered questionnaire, and a database was established by Excel 2016 for descriptive statistical analysis. Copies of 211 application evaluation questionnaire and 211 copies of applicability evaluation questionnaire were collected. We can conclude from the survey that we should adjust the whole content and structure on the basis of better evaluation of the present recommendation scheme, update the prescription selection and clinical evidence of the recommendation scheme, and put forward the improvement measures for the hindrance factors in the application of the Guideline. Furthermore, in order to promote the Guideline more clearly, we should strengthen the doctor-patient education, improve guidance quality and increase the publicity, providing basis for the implementation and promotion strategies of the Guideline., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Chinese Pharmaceutical Association.)
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- 2018
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14. [Optical Properties of ZnO Films Fabricated by Atomic Layer Deposition].
- Author
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Zhang CM, Wang DD, Fang M, Zhang A, Wang XY, Chen Q, and Meng T
- Abstract
The ZnO films were deposited by atomic layer deposition method using water and diethylzinc as precursors at different temperatures (110 and 190 degrees C). X-ray photoelectron spectroscopy, spectroscopic ellipsometry and photoluminescence spectra (PL) were used to investigate the elemental composition and optical properties of ZnO films. Our results showed that with the increasing of the growth temperature, the amount of -OH groups in the ZnO film decreased, which indicated that the reactions went to completion at high processing temperatures. The PL spectra of the ZnO film deposited at 110 degrees C exhibited two emission bands, one in the UV region and the other in the visible region. When the deposition temperature increased to 190 degrees C, the emission bands in the visible region disappeared, which indicated that the deep level defect in ZnO became less. The carrier mobility improved from 25 to 32 cm2 x (V x S)(-1) with the reduction of the defects in the ZnO film. The refractive index of the ZnO films decreased from 2.33 to 1.9 in the 375-800 nm region. The optical absorption edge (E(g)) values of the ZnO films deposited at different temperature were about 3.27 eV.
- Published
- 2016
15. [Quality standard of uygur medicine Medicago sativa seeds].
- Author
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Ding WH, Xu HY, Wang DD, Li J, and Tian SG
- Subjects
- Alkaloids analysis, Alkaloids standards, Chromatography, High Pressure Liquid, Drugs, Chinese Herbal standards, Medicago sativa ultrastructure, Quality Control, Seeds chemistry, Seeds ultrastructure, Drugs, Chinese Herbal analysis, Medicago sativa chemistry
- Abstract
In this paper, microscopic identification method was adopted to observe the microscopic characters of ten batches of Medicago sativa seeds. And M. sativa seeds were identificated by TLC method in contrast to trigonelline and stachydrine hydrochloride. The impurities, moisture, ash, sour insoluble ash were detected based on Chinese Pharmacopoeia 2010 version (Vol I ). An HPLC method was also established for determination of trigonelline in the M. sativa seeds. The contents of impurities, moisture, ash, sour insoluble ash should not exceed 5%, 10%, 6%, and 2%, respectively. The content of trigonelline should be not less than 0.795 6 mg x g(-1). The experimental methods were accurate and reliable, and can be used as the quality control of the seeds of M. sativa.
- Published
- 2013
16. [A preliminary report of apoA5 gene novel receptor-binding domain mutation in a patient with severe hypertriglyceridemia].
- Author
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Wang XL, Wang DD, and Zhang J
- Subjects
- Adult, Apolipoprotein A-V, Child, Preschool, Female, Genotype, Heterozygote, Humans, Male, Middle Aged, Apolipoproteins A genetics, Hypertriglyceridemia genetics, Mutation
- Abstract
Objective: To find novel variants and explore potential mechanism of triglyceride metabolism by resequencing apoA5 gene in patients with severe hypertriglyceridemia., Methods: The apoA5 gene in the patients with severe hypertriglyceridemia were resequenced, who had been excluded with variants in LPL or apoCII. With constructed apoA5 cDNA expression vectors, transiently expression was observed in vitro and the protein expression was detected by Western blot and (35)S-labeled immunoprecipitation., Results: The mutations of T184S and V153M were found in a patient with heterozygous variants in apoA5, and T184S was not reported before. Both mutant and wild type apoA5 cDNA expression vectors were constructed successfully. Western blot and immunoprecipitation showed they can be expressed in the cell and the mutational apoA5 can be secreted from the cell as well as wild type., Conclusion: A novel mutation in apoA5 was found in a patient with severe hypertriglyceridemia, which has the potential damaging effect for the function of this protein but not the secretion function. It need further study on the interaction of apoA5 and other related proteins.
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- 2012
17. [Role of microRNA-223 and its target gene oncogene c-myc in hepatocellular carcinoma pathogenesis].
- Author
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Zhao WY, Wang DD, Song MQ, Yang L, Ye J, and Chen LB
- Abstract
To investigate the regulatory role of microRNA-223 (miR-223) on c-myc and its role in hepatocarcinogenesis. miR-223 and c-myc mRNA expressions in normal tissue, paraneoplastic tissue, liver cancer tissue and liver cancer cells were tested with microRNA microarray and quantitative real-time PCR (qRT-PCR). C-myc protein expression was detected by Western blot. MiR-223 mimic was transfected into HepG2 cells and the expression changes of c-myc mRNA and protein were tested with qRT-PCR and Western blot respectively. MiR-223 was down-regulated by 61.53% and 30.77% respectively in hepatocellular carcinoma and adjacent tissues as compared to normal liver tissues and the expression of miR-223 was also decreased in HepG2 cell as compared to fetal liver cells L02, whereas the expressions of c-myc mRNA and protein increased in paraneoplastic and HCC tissues compared with normal liver tissues. It prompts that the expressions of miR-223 and c-myc are negatively correlated. No obvious difference found among c-myc mRNA expressions after miR-223 mimics transfection. The c-myc abnormal high-expression may play a dynamic role in hepatocarcinogenesis due to the miR-223 down-regulation.
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- 2011
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18. [Studies on the chemical constituents from pine needles of Cedrus deodara (II)].
- Author
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Zhang JM, Shi XF, Ma QH, He FJ, Wang DD, Liu DY, and Fan B
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- Dibutyl Phthalate chemistry, Flavones chemistry, Flavones isolation & purification, Glycosides chemistry, Glycosides isolation & purification, Hydroxybenzoates chemistry, Molecular Structure, Plant Leaves chemistry, Triterpenes chemistry, Cedrus chemistry, Dibutyl Phthalate isolation & purification, Hydroxybenzoates isolation & purification, Triterpenes isolation & purification
- Abstract
Objective: To study the chemical constituents from pine needles of Cedrus deodara., Methods: Chemical constituents were isolated from 95% ethanol extract by silica gel and Sephadex LH-20 column chromatography. The structures were elucidated through spectroscopic analysis., Results: The compounds were identified as 10-nonacosanol (1), dibutyl phthalate (2), protocatechuic acid (3), phthalic acid bis-(2-ethylhexyl) ester (4), (E)-1-O-p-coumaroyl-beta-D-glucopyranoside (5), 5-p-trans-coumaroylguinic acid (6)., Conclusions: Compounds 1- 6 are obtained from this genus for the first time.
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- 2010
19. [The enhancement of electric field distribution in one-dimensional metallic-dielectric photonic crystals].
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Deng LE, Wang YS, Wang DD, Xu Z, Fu M, He DW, Zhao AL, and Tao YL
- Abstract
The authors theoretically study the transmission properties and electric filed distribution in one-dimensional metallic-dielectric photonic crystals with the transfer matrix method. The results show that the physical properties can be improved considerably after the introduction of antireflection layer and optimizing structural parameters, e. g., 77% electric file distribution in metal layers can be obtained under 19% transmittance, and 72% transmittance can be obtained with 28% electric field distribution in metal layers. These photonic crystal structures, whose transmission properties and electric field in metal layers are tunable, could be widely used in nonlinear photon device based on the enhancement of electric field in metal layers.
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- 2010
20. [A prospective comparison between surgery alone and postoperative chemoradiotherapy for locally advanced esophageal squamous cell carcinoma].
- Author
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Cao XF, Lü J, Zhu B, An HY, Wang S, Wu BC, Ji L, Tao L, and Wang DD
- Subjects
- Adult, Aged, Carcinoma, Squamous Cell pathology, Chemotherapy, Adjuvant, Cisplatin administration & dosage, Disease-Free Survival, Esophageal Neoplasms pathology, Esophagectomy methods, Female, Follow-Up Studies, Humans, Lymph Node Excision, Lymphatic Metastasis, Male, Middle Aged, Neoplasm Recurrence, Local, Neoplasm Staging, Paclitaxel administration & dosage, Prospective Studies, Radiotherapy, Adjuvant, Survival Rate, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Carcinoma, Squamous Cell therapy, Esophageal Neoplasms therapy, Radiotherapy, High-Energy
- Abstract
Objective: To investigate the role of postoperative chemoradiotherapy (CRT) as a multimodality treatment option for locally advanced thoracic esophageal squamous cell carcinoma (ESCC) by a prospective comparison between surgery alone and postoperative CRT., Methods: Using preoperative computed tomography (CT)-based staging criteria, 158 patients with ESCC (stage II-III) were enrolled in this prospective study. With informed consent, the patients were randomized into two groups: postoperative CRT (78 cases) and surgery alone (S, 80 cases). After a few minor adjustments to the enrolled patients, the actual patients of postoperative CRT group and S group were 74 cases and 77 cases, respectively. Comparison of the complications, local recurrence rate, distant metastasis rate, survival rate and progression-free survival in the two groups was carried out., Results: With a median follow-up of 37.5 months, the 1-, 3-, 5-, 10-year overall survival (OS) rates were 91.0%, 62.8%, 42.3%, 24.4% and 87.5%, 51.3%, 33.8%, 12.5% for the postoperative CRT and S arm, respectively. A significant difference in OS was detected between the two arms (P = 0.0276). There was a significant difference of progression-free survival (PFS) between the two arms (P = 0.0136). The local recurrence rates in the postoperative CRT group and S group were 14.9% and 36.4%, respectively (P < 0.05). No significant difference was detected between the complications of the two groups (P > 0.05). Toxicities of chemoradiotherapy in the postoperative CRT arm were moderate, which can be relieved rapidly by adequate therapy., Conclusion: Rational application of postoperative chemoradiotherapy can provide a benefit in progression-free survival and overall survival in patients with locally advanced esophageal squamous cell carcinoma.
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- 2010
21. [Expressions of HLA class I antigen and CD8 and their clinical significance in cervical cancer].
- Author
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Qi Y, Huang JS, Wang DD, Zhang F, and Zhang SL
- Subjects
- Adult, Aged, CD8 Antigens metabolism, Down-Regulation, Female, Histocompatibility Antigens Class I metabolism, Humans, Middle Aged, Uterine Cervical Neoplasms pathology, Uterine Cervical Dysplasia pathology, CD8 Antigens immunology, Histocompatibility Antigens Class I immunology, Uterine Cervical Neoplasms immunology, Uterine Cervical Dysplasia immunology
- Abstract
Objective: To examine the expressions of HLA class I antigen and CD8 in various cervical diseases and investigate their association with cervical cancer., Methods: The expressions of HLA class I antigen and CD8 in cervical tissues sampled from patients with cervical cancer, cervical intraepithelial neoplasia (CIN), and chronic cervicitis were detected using SP immunohistochemistry. The association of the expressions of HLA class I antigen and CD8 with the clinicopathologic indices of the patients was analyzed., Results: The positive expression rates of HLA class I antigen in cervical cancer, CIN, and chronic cervicitis were 22.6%, 100.0%, and 100.0%, and the positive expression rates of CD8 were 22.6%, 95.5%, and 100.0%, respectively. The positive rates of HLA class I antigen and CD8 were significantly lower in patients with cervical cancer (P<0.01). Patients with stage I cervical cancer had significantly higher positive rates of HLA class I antigen and CD8 than those with stage II cervical cancer (46.7% vs 0.0%, 46.7% vs 0.0%, both P<0.01). The expressions of HLA class I antigen and CD8 decreased with the progression of the clinicopathological stages, and may even become undetectable. The expressions of HLA class I antigen and CD8 were not related to the differentiation degree of the tumor or lymph node metastasis (P>0.05). A positive correlation was found between HLA class I antigen expression and CD8 expression., Conclusion: The expressions of HLA class I antigen and CD8 are down-regulated or deleted in CIN and cervical cancer, and they may play important roles in the development and progression of CIN and cervical cancer.
- Published
- 2008
22. [Transfection of alpha1, 2-fucosyltransferase gene increases the antigenic expression of Lewis y in ovarian cancer cell line RMG-I].
- Author
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Lin B, Hao YY, Wang DD, Zhu LC, Zhang SL, Saito M, and Iwamori M
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- Cell Line, Tumor, Chromatography, Thin Layer, Female, Fucosyltransferases genetics, Humans, Fucosyltransferases physiology, Lewis Blood Group Antigens metabolism, Ovarian Neoplasms metabolism, Transfection methods
- Abstract
Objective: To transfect human alpha1, 2-fucosyltransferase (alpha1, 2-FT) gene to ovarian cancer cell line RMG-I and investigate the antigenic expression change of Lewis y and the other related oligosaccharides., Methods: The expression vector pcDNA3.1(-)-HFUT-H was constructed by polymerase chain reaction (PCR) to clone human alpha1, 2-FT gene coding region. The alpha1, 2-FT gene stable high-expression cell line RMG-I-H was established by transfecting pcDNA3.1(-)-HFUT-H to ovarian cell line RMG-I. The change of alpha1, 2-FT activity in the cell line before and after the tranfection was confirmed by the determination of enzymatic activity. The changes of cell lipid and glucolipid, especially the change of type II oligosaccharide, in the cell line before and after the transfection was determined by Thin-Layer Chromatography (TLC) and TLC immunostaining method, respectively., Results: The H-1 antigen and Lewis y antigen were obviously increased in the cell line RMG-1-H, especially the latter one, which was 20 times higher than before, and the type I saccharide chain Lewis b was decreased significantly. The main lipid components on the cell membrane, cholesterol and phosphatides, showed no change in the cell lines before and after the transfection, and the neutral glycolipid also showed no obvious change., Conclusions: The transfection of alpha1, 2-FT gene can increase the activity of alpha1, 2-FT in the cell line RMG-I and mainly increase the expression of Lewis y antigen simultaneously. The construction of RMG-I Lewis y high expression cell line provides a cell model for further study on the relationship between Lewis y antigen and biological behaviors in the ovarian cancer.
- Published
- 2008
23. [Spectral properties of two-dimensional photonic crystal quantum well structures].
- Author
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Wang DD, Wang YS, Xu Z, Deng LE, Zhang CX, and Han X
- Abstract
In the present paper, the spectral properties of two-dimensional (2D) photonic crystal quantum well structures were studied numerically. The structures consist of a 2D photonic crystal (PC) with square lattice of parallel dielectric circular columns in air and some middle layers of columns are removed. Similar to the electrons in semiconductor quantum wells, the photonic bandgap (PBG) in PC can act as a potential barrier to photons, which gives rise to quantized photonic states in the PBG region. Photonic band structures were calculated using plane wave expansion method and transmission spectra were obtained using transfer matrix method. The results show that discrete transmission peaks appear in PBG region. More transmission peaks arise with the increase of the well layer and the strength decreases with the increase in the potential layer width. The relationships between the frequency of transmission peaks and the width of well layer were also discussed.
- Published
- 2008
24. [Optical properties of one-dimensional metallodielectric photonic crystals].
- Author
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Luo YH, Wang YS, Wang DD, Deng LE, and Sun J
- Abstract
The optical characteristics of one-dimensional metallodielectric photonic crystals (MDPC), constructed by inserting metal aluminum layers of certain thickness into the Si/SiO2 system, were studied theoretically with the transfer matrix method. The results show that the reflection efficiency can be enhanced considerably after the introduction of metallic layers, e. g. the rejection level of each period increased from 7.2 dB([Si(46 nm)/SiO2 (120 nm)]5) to 20 dB ([Si(46 nm)/SiO2 (60 nm)/Al (10 nm)/SiO2 (60 nm)]5). In addition, high omnidirectional reflection band with broader width can be obtained, e. g. bandwidth of 550 nm can be offered with [Si(46 nm)/SiO2 (60 nm)/Al (30 nm)/SiO2 (60 nm)]5. Rules of how the absorption, thickness and position of the metal layers affect the optical characteristics of the MDPC are also discussed. These MDPC structures may be used as compact-size, low-loss and broad-band optical reflectors.
- Published
- 2008
25. [Interaction of ctDNA and EGCG-Cu(II) complex].
- Author
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Wang DD, Meng QX, Chen DD, Ren LP, Zhou ZM, and Mu HJ
- Subjects
- Animals, Catechin chemistry, Cattle, Hydrogen-Ion Concentration, Molecular Structure, Spectrometry, Fluorescence, Spectrophotometry, Catechin analogs & derivatives, Copper chemistry, DNA chemistry
- Abstract
The fluorescence spectra, absorption spectra and resonance light scattering (RLS) spectra of EGCG-Cu((II) complex with nucleic acid were studied. Compared with the spectrum of EGCG-Cu(II), the spectrum of EGCG-Cu(II)-DNA system showed the following features: (1) in the fluorescence spectra, the peak did not shift, but the fluorescence intensity was enhanced and was proportional to the concentration of DNA. Under suitable conditions, EGCG-Cu(II) promised to be a probe for detecting DNA; (2) Absorption spectra of EGCG-Cu(II)-DNA system showed hyperchromicity; and (3) RLS of EGCG-Cu(II)-DNA system was enhanced also. A mechanism was suggested that the combination of nucleic acid and EGCG-Cu(II) complex was caused by intercalation and electrostatic force. An intercalation interaction between EGCG-Cu(II) and DNA enhanced the fluorescence intensity. Hyperchromicity of absorption spectra and RLS occurred because of electrostatic force. The effects of acid and ionic strength on fluorescence intensity were also discussed.
- Published
- 2007
26. [Chip-based capillary electrophoresis on-line coupled with atomic fluorescence spectrometry and its application to rapid speciation analysis].
- Author
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Li F, Wang DD, Yan XP, Su RG, and Lin JM
- Abstract
Chip-based capillary electrophoresis (chip-CE) is under rapid progress due to its high efficiency, fast separation, little sample consumption and integration. Due to the short optical path (30-50 microm), the conventional UV absorbance cannot satisfy the chip detection needs. Atomic fluorescence spectrometer (AFS) is a selective and sensitive detector for hydride-forming elements. With the strong points of low cost and easy operation, AFS is attractive as an ideal detector of chip-CE for mercury, arsenic and selenium, etc. A few important practical aspects of coupling chip-CE to AFS are critically discussed. These include the design of the chip, chip-AFS interface, gas-liquid separator, AFS atomizer, and elimination of the effect of backpressure on separation. The application of the developed chip-CE-AFS to the mercury speciation analysis is highlighted.
- Published
- 2006
27. [Study on the resonance scattering light spectra of atrazine-RNA and its analytical application].
- Author
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Ren LP, Jiang SR, Rao ZH, Xiong YM, Zhang CR, and Wang DD
- Subjects
- Atrazine toxicity, Calibration, Herbicides toxicity, Light, Limit of Detection, Spectrum Analysis, Vibration, Atrazine chemistry, Food Contamination, Herbicides chemistry, RNA chemistry, Saccharomyces cerevisiae chemistry, Scattering, Radiation
- Abstract
The Resonance Light Scattering (RLS) spectra of atrazine with yeast ribonucleic acid (yRNA) had been studied. At pH 1.50, the enhanced RLS of atrazine-yRNA system is characterized by the maximum scattering peaks at 320 nm and the RLS intensity is proportional to the concentration of yRNA. Under optimal conditions, the linear ranges of the calibration were 0.6-5.0 microg x mL(-1), the linear regression equation was I = 25.88 + 140.0 c(yRNA, microg x mL(-1)), r = 0.9975, with the limit of detection 20.7 ng x mL(-1) (3delta). The method was applied to the determination of yRNA in synthetic samples and RNA in salt cress (Thellungiella halophila) sample satisfactorily. Mechanism studies show that there are two interaction modes between atrazine and RNA: electrostatic mode and intercalative mode.
- Published
- 2004
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