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Your search keyword '"WEN YAN YU"' showing total 2 results
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2 results on '"WEN YAN YU"'

1. Adverse events with regional citrate anticoagulation versus heparin anticoagulation during continuous renal replacement therapy: a systematic review and trial sequential analysis

Author

Min-min Wang, Marshall Mark, Wen-yan Yu, and Qiang Yao

Subjects
continuous renal replacement therapy, regional citrate anticoagulation, heparin, Internal medicine, RC31-1245
Abstract

Objective To compare adverse events (AEs) of regional citrate anticoagulation (RCA) versus heparin anticoagulation during continuous renal replacement therapy (CRRT) through systematic review and trial sequential analysis. Methods Based upon the relevant randomized control trials (RCT) comparing RCA and heparin anticoagulation during CRRT in both domestic and foreign databases, the authors re-analyzed various AEs including at least one of the following episodes: hypocalcemia, metabolic acidosis, metabolic alkalosis, hemorrhage and heparin-induced thrombocytopenia (HIT). Meta-analysis was performed with Revman 5.2 and TSA 0.9 software for trial sequential analysis (TSA). Results Among a total of 791 items, 18 articles were included. As compared with heparin, RCA lowered the frequency of overall AEs (RR=0.54, 95%CI:0.35-0.82, P=0.004). And hemorrhagic risk dropped by 73% (RR=0.27, 95%CI:0.19-0.38, P

Published
2023
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2. Construction of a eukaryotic expression plasmid pcDNA3.1-HuR-FLAG and its transient expression in NIH3T3 cells

Author

Tao LI, Wen-yan YU, Xiao-li OU, Ai-hua LIU, Zhu-zhong MEI, and Yong JIANG

Subjects
human antigen R； gene expression； posttranscriptional regulation, lcsh:R5-920, lcsh:R, lcsh:Medicine, lcsh:Medicine (General)
Abstract

Objective To construct a eukaryotic expression vector for HuR and analyze its expression and biological function in NIH3T3 cells.Methods The total RNA was extracted from NIH3T3 cells and reverse transcribed to cDNAs.The coding region sequence of mouse HuR was then amplified by PCR and subcloned into the pcDNA3.1-FLAG plasmid.The recombinant plasmid pcDNA3.1-HuR-FLAG was verified by PCR and restriction endonuclease analysis,confirmed by DNA sequence analysis,and then transiently transfected into NIH3T3 cells with Lipofectamine LTX.The expression of HuR protein was determined by Western blotting,and the mRNA level of HuR and DUSP1 were analyzed by using real-time PCR.Result The recombinant plasmid pcDNA3.1-HuR-FLAG was correctly constructed.Twenty-four hours after transfection of the recombinant plasmid into NIH3T3 cells,the fusion protein was found to have highly expressed in the cells as revealed by Western blotting.Real-time PCR results detected that the over-expression of HuR could up-regulate the expression of DUSP1.Conclusion The eukaryotic expression vector for HuR-FLAG fusion protein has been successfully constructed and transiently expressed in NIH3T3 cells.It can be used in further analysis of the posttranscriptional regulation of DUSP1 by HuR in cancer cells.

Published
2011

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