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PURPOSE: To explore the effectiveness of Osteopontin siRNA (OPNsiRNA) on viability and invasiveness of Tea 8113 by attenuated Salmonella typhimurium. METHODS: Synthesised OPNsiRNA and nonsense ControlsiRNA were separately constructed into expression vector pIRES2-EGFP, and were transferred into attenuated Salmonella SL7207. The tongue cancer cell line Tea 8113 was infected by the recombinant Salmonella SL7207, named SL-OPNsiRNA and SL-ControlsiRNA respectively. The infection rate, growth, apoptosis, migration and invasion were observed while the protein expressions of OPN, MMP-2 and uPA were detected by Western blot. SPSS15.0 software package was used for statistical analysis. RESULTS: The infection rates of the recombinant Salmonellas both were more than 80.0% without significant difference (P>0.05). The growth was significantly inhibited and the apoptosis was significantly induced after infection of SL-OPNsiRNA compared with SL-ControlsiRNA. After 24h-infection of SL-OPNsiRNA and SL-ControlsiRNA, the migration index was 0.56±0.10 and 0.82±0.05 (n=12, P<0.01), the invasion index were 0.53±0.13 and 0.83±0.06 (n=12, P< 0.01),respectively, and the intra -cell proteins of OPN, MMP -2 and uPA were also significantly down -regulated by OPNsiRNA compared with ControlsiRNA. CONCLUSIONS: OPNsiRNA could be effectively transferred into tongue cancer cells through attenuated Salmonella, and inhibited caner cell growth and metastasis. Supported by National Natural Science Foundation of China (30572060) and Science Foundation of Nanjing BenQ Hospital (SRD20100003). [ABSTRACT FROM AUTHOR]