【Objective】The study aimed to explore effects of Pb as interaction on emergence time, emergence rate, tiller number, lead and arsenic accumulation of highland barley under the condition of Pb and as compound pollution, which was of great significance for the prevention and control of soil heavy metal pollution and the protection of human health. 【Method】Taking highland barley as the test crop, 27 treatments were set up with different concentrations of single factor Pb(0, 480, 720, 960, 1200, 1440 mg/kg), As(0, 30, 60, 90, 120, 140 mg/kg), Pb(480, 720, 960, 1200 mg/kg) and As(30, 60, 90, 120 mg/kg). Pot experiments were conducted to study the effects of Pb as interaction on the growth and heavy metal accumulation of highland barley. 【Result】The interaction of lead and arsenic showed different degrees of inhibitory effect on the emergence time, emergence rate, tiller number and heading time of highland barley, that was, compared with the blank, lead and arsenic had obvious inhibitory effect on the growth and development of highland barley. The accumulation ability of highland barley seedlings to lead was stronger than that of arsenic. Highland barley has stronger tolerance to lead. When the concentration of arsenic was too high, highland barley will stop growing or even die. When the concentrations of 480, 720 and 1200 mg/kg of lead interacted with arsenic, the accumulation of lead in highland barley seedlings was antagonistic to that of arsenic. When the concentration of lead was 960 mg/kg, the interaction between arsenic and lead was not obvious. When the concentration of arsenic was 30 and 120 mg/kg, the accumulation of arsenic in highland barley seedlings was antagonistic to lead. When the arsenic concentration was 60 and 90 mg/kg, the interaction between arsenic and lead was not obvious. 【Conclusion】The interaction of lead and arsenic had an effect on the growth of highland barley seedlings. The interaction of high content of lead and arsenic significantly inhibited the growth of highland barley seedlings. The results showed that the emergence rate and tiller number of highland barley were significantly decreased, and the emergence time and heading time were significantly increased under the interaction of high concentration of lead and arsenic. The interaction of lead and arsenic showed antagonistic effect on the accumulation of lead and arsenic in highland barley seedlings. [ABSTRACT FROM AUTHOR]