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Objective: To investigate the prevalence of trematode Aspidogastrea in freshwater mussels in Anhui Province, China., Methods: The freshwater mussels living in different water areas within Anhui territory were harvested and dissected from April to May, 2015. Then the flukes were collected from the pericardial cavities of the clams into a petri dish containing small amount of saline. After rinsing, the flukes were transferred into a bottle containing 70% ethanol for following identification by staining of the specimens prepared as previous protocol., Results: A total of 3 007 calms in 12 species, including Unio douglasiae (603), Acuticosta chinensis (90), Lamprotula caveat (150), Lamprotula leai (250), Lanceolaria grayana (47), Anodonta pacifica (60), Anodonta woodiana (350), Cuneopsis heudei (100), Solenaia oleivora (150), Cristaria plicata (567), Hyriopsis cumingii (550) and Arconaia lanceolata (90) were dissected, in which 1 467 mussels were infected with the trematode Aspidogastrea, with an infection rate of 48.79% (1 467/3 007). Totally, 7 306 flukes belonging to 3 genera under the subclass of Aspidogastrea were isolated. These trematodes were genera of Aspidogaster , Lophotaspis and Cotylaspis . The infection density was from 1 to 128 capita for individual positive clam, with a mean infectiosity of 4.98 (7306/1467) for each mussel., Conclusions: The freshwater bivalves living in different water areas in Anhui Province are infected with the trematode Aspidogastrea. These flukes were identified as Aspidogaster sp. Lophotaspis sp. and Cotylaspis sp. belonging to subclass Aspidogastridae of family Aspidogastrea.

Objective: To explore the existence of natural foci of Metorchis taiwanensis in Wuhu area., Methods: The ecological environment and daily living habits of residents were investigated; the eggs of M. taiwanensis in the dung of ducks in local region were collected by the sedimentation method; the Parafossarulus striatulus were disposed by the tabletting method and examined with a microscope to isolate the rediae and cercariae; the metacercariae in Pseudorasbora parve were isolated by using the homogenation. The duckling were fed directively by metacercaria of M. taiwanensis or by P. parve infected with metacercaria of M. taiwanensis for artificial infection, and M. taiwanensis were separated from ducks by dissecting the gallbladder and bile ducts., Results: The survey revealed that there were staggered lake river, rich vegetation, overgrown shrubs, aquatic plants, rich variety of freshwater snails and fish resources in Wuhu area, and it was home to a variety of waterfowl and duck. The average detection rate of eggs of M. tanwanensis in stool of ducks was 33.33% (10/30); that of rediae and cercarriae in P. striatulus was 1.17%(7/600); and that of encysted metacercaria in P. parve was 13.33% (8/60); the number of M. taiwanensis detected in the infected ducklings in 3 groups were 31, 8 and 0, respectively, the natural M. tanwanensis infection rate of ducks was 23.33% (7/30)., Conclusion: We have confirmed the existence of natural foci of M. tanwanensis in Wuhu area.

40 days after oral infection of rat with metacercariae of Pagumogonimus skrjabini, a few worms matured sexually in the abdominal cavity or worm-cysts formed in the lungs, while juveniles were detected in the muscles. 60-90 days after infection, worm-cysts can not only be detected in the lungs, but also in the liver, mediastinum and abdominal wall, with mature or premature worms and eggs. It is suggested that P. skrjabini could mature in rat and showed a phenomenon of polyorganotropism. By means of host-transfer with small juveniles, a part of them migrated to the lungs and matured, but the worms detected in muscles were still stunted.