Search

Due to outstanding advantages such as lightweight, good toughness, low cost and simple forming process, reduced graphene oxide (rGO) films have attracted considerable attention in temperature sensing and electro heating fields. In this study, the difference in the microstructure, bond types, mechanical and electrical properties of rGO films reduced at different temperatures are analysed to explore the influence of reduction degree on temperature sensing and heating performance of rGO films. The results indicate that oxygen-containing functional groups including C-O and C=O in graphene oxide (GO) films decrease dramatically at 200 ℃, and the main bonding type of carbon atoms transfers from C-C to C=C. When the reduction temperature rises to 600 ℃, rGO films still retain some oxygen-containing functional groups with a C/O ratio of 7.18, while the composition of rGO films reduced at 800 ℃ approaches to graphene. With the increase of reduction degree, electrical resistivity of rGO films shows a significant decrease. However, the outgassing of oxygen atoms disrupts the interlayer dense structure, leading to gradual reduction in tensile strength. At the reduction temperature of 600 ℃, the resistance-temperature curve exhibits excellent linear relationship with a temperature coefficient of resistance (TCR) value of -1.60×10-3/℃ at room temperature. Under 24 V DC driving voltage, rGO films reduced at 600 ℃ and 800 ℃ reach 242 ℃ and 367 ℃, respectively. However, due to the loose and cracked interlayer structure, there happens a noticeable fluctuation in the heating temperature curve of rGO films reduced at 800 ℃. Therefore, rGO films reduced at 600 ℃ are proven to be more suitable for heating and temperature sensing. [ABSTRACT FROM AUTHOR]

Autoimmune disease (AID) is a disease in which the body loses tolerance to autoantigens and the immune system attacks its own tissues, resulting in organ and tissue damage and certain clinical manifestations. Studies have proved that pathogenic T cells can be selectively inhibited or killed by TCR vaccine in the treatment of AID, thus bringing broad application prospect. This paper briefly summarizes the research status of TCR vaccine in AID field, regarding action mechanism, related diseases and safety of TCR vaccine. [ABSTRACT FROM AUTHOR]

Objective: Using single cell sequencing to analyze the characteristics of the immune repertoire of SARS-CoV-2 patients at different infection stages, and to explore the possible pathogenesis. Methods: Obtaining data in the NCBI database, and healthy control, progression and convalescence groups were set up. Analysis of single cell sequencing data by RStudio, Origin, Hipliot and Excel software. Results: TCR plays an important role in antigen recognition and virus clearance. Characteristics of the three immune repertoires are very different. The number of clones exceeded 26.92%. The length distribution of α chain CDR3 was concentrated on 14 amino acids, while β-chain was concentrated on 15 amino acids. The frequency of gene fragments in V and J regions were different, and the frequency of TRAV13-1, TRAJ20, TRBV20-1 and TRBJ2-1 were statistically significant (P<0.05). Further V-J gene combination analysis showed that there were significant differences in the frequency of single chain V-J between the control group and the progression group, the control group and the rehabilitation group (P<0.05). The highest frequency of αβ double chain V-J in the control group was TRAV19-J34-TRBV4-1-J2-1, the highest frequency of αβ double-stranded V-J in the progression group was TRAV12-1-J30-TRBV19-1-J2-1, and the highest frequency of αβ double chain V-J in the convalescence group was TRAV12-2-J52-TRBV7-9-J1-5. Conclusion: This study analyzes the global characteristics of T cells in the immune repertoire, which is helpful to understand the pathogenesis of SARS-CoV-2 patients, timely and effective treatment of patients in the early stages of infection. [ABSTRACT FROM AUTHOR]

Objective: To analyze differences in peripheral blood T-cell receptor （TCR） genes in patients with viral pneumonia by single cell sequencing, and to explore its possible pathogenesis. Methods: Data in NCBI database was obtained, and case and control groups were set up for experiments. Single cell sequencing data was analyzed of by R language in RStudio software, tables and images were plotted, and finally conclusions were drawn. Results: There was a significant difference in TCRs between case and healthy control groups. Frequency of use of V and J gene fragments of TCR α and β chains were analyzed, in which frequency of use of TRAV1-2, TRAV29/DV5, TRAJ33, TRAJ48, TRBV20-1, TRBV2, TRBJ2-3 and TRBJ1-1 genes were significantly higher in case group than in control group（ P<0.05）. Further V-J gene combination analysis showed that the most frequent use of α-chain V-J pairs in case group was TRAV1-2-J33, most frequent use of β-chain V-J pairs was TRBV20-1-J2-1, most frequent use of αβ double-chain V-J pairs was TRAV30-J24-TRBV3-1-J2-7. Conclusion: Peripheral blood TCR genes are significantly altered in patients with viral pneumonia, which may be related to immunopathogenesis of virus. [ABSTRACT FROM AUTHOR]

Copyright of Environmental Science & Technology (10036504) is the property of Editorial Board of Environmental Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Electronic Components & Materials is the property of Electronic Components & Materials and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

【目的】探讨再生障碍性贫血（再障）儿童TCR Vβ24个亚家族T细胞克隆性及其与HLA—DRB1＊15的关系。【方法】再障儿童17例（SAA14例，MAA3例），采用RT—PCR和基因扫描分析外周血或骨髓TCR Vβ24个亚家族基因的表达和克隆性，SSP—PCR检测HLA—DR。【结果】再障儿童外周血T细胞仅表达4—22个Vβ亚家族，而正常儿童外周血T细胞几乎表达所有Vβ亚家族。12例（包括初诊SAA4例，CR4例，复发1例和MAA3例）再障儿童存在不同程度T细胞克隆性增殖，但个体间差异较大，正常外周血和骨髓均为多克隆性T细胞。5例HLA—DRB1＊15（＋）患儿中4例（80％）有寡克隆T细胞，而12例HLA—DRB1＊15（-）患儿中仅3例（25％）见寡克隆T细胞，两组比较差异具有显著意义（P〈0．05）。伴寡克隆T细胞的6例S从儿童经免疫抑制治疗后达CR；不伴寡克隆T细胞的5例SAA儿童接受免疫抑制治疗，其中CRI例，PR2例、无效1例，死亡1例。【结论】大多数再障儿童存在T细胞克隆性增殖，寡克隆T细胞多见于SAA，尤其是HLA—DRB1＊15（＋）的SAA儿童。TCR VβT细胞克隆的检测对进一步了解再障免疫功能状态、预测免疫抑制治疗效果具有一定的参考价值。

To explore the immune status of patients with drug-induced liver injury caused by Polygonum multiflorum preparations,and analyze their immune characteristics. Case-control design was used to collect the cases of drug-induced liver injury caused by P. multiflorum preparations through key specialized surveillance. Five matching factors,namely type of P. multiflorum preparations,gender,age,basic diseases and concomitant medication were controlled. According to the ratio of 1 ∶ 1,cases of patients who took P. multiflorum preparations but with no liver injury were monitored at prospective hospitals. The demographic information,disease information,medication information and laboratory examination information of the two groups were recorded,and venous blood was collected. The gene sequence was detected by high-throughput sequencing technology,and the characteristics of TCR immune repertoire of the two groups were analyzed. A total of 46 pairs of patients were enrolled in the study. The results showed significant differences in the number of CDR3 and clone species,the length of amino acid sequence in CDR3 region,the abundance of V gene and J gene,the cross-linking of V-J gene and the diversity of immune repertoire between patients with drug-induced liver injury and patients without liver injury. The immunohistochemical diversity and high-frequency V-J cross-linking characteristics of patients with liver injury caused by P. multiflorum preparations were found,which provided a reference for screening out drug users to reduce the occurrence of liver injury caused by P. multiflorum preparations.