39 results on '"Tang, Juan"'
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2. Transcriptome analysis based on the characteristic difference between Shatang tangerine and Shatang Lantern tangerine.
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TANG Juan, WANG Feiyong, and ZHANG Lan
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- 2023
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3. Application of structural psychological education intervention based on timing it right in patients with liver cancer and depression.
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ZHAO Fengjuan, REN Yunhong, WEI Jueling, ZHAO Xinhua, TANG Juan, MA Liang, and YOU Xuemei
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- 2020
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4. Application value of feeding strategy based on volume-viscosity swallow test in the management of dysphagia due to cerebral hemorrhage.
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TANG Juan-hui, ZHENG Ying-na, and OU Yao
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LENGTH of stay in hospitals ,CEREBRAL hemorrhage ,DEGLUTITION ,ARTIFICIAL feeding ,DEGLUTITION disorders ,ASPIRATION pneumonia ,FOOD portions ,TREATMENT effectiveness ,COMPARATIVE studies ,MALNUTRITION ,DESCRIPTIVE statistics ,VISCOSITY ,ENTERAL feeding ,DISEASE complications ,EVALUATION - Abstract
Objective To explore the application value of feeding strategy based on volume- viscosity swallow test (V-VST) in the management of swallowing disorders in cerebral hemorrhage. Methods 150 cases of cerebral hemorrhage patients with dysphagia in Guangzhou First People's Hospital from October 2017 to January 2020 were observed. Patients who received routine feeding from October 2017 to October 2018 were included as the control group (n =70). Patients with V- VST-based feeding strategy from October 2018 to January 2020 were included as the observation group (n = 80). The incidence of complications related to dysphagia and drinking water test were compared between the two groups, and the time of tube feeding and hospital stay were recorded. Results The complication rates of dysphagia aspiration, aspiration pneumonia and malnutrition in the observation group were significantly lower than those in the control group (all P < 0. 05). The tube feeding time and hospital stay of the observation group were significantly shorter than those of the control group (both P < 0. 05). The percentage of patients with grade 1 and grade 2 in drinking water test in the observation group was higher than that in the control group (P < 0.05). Conclusion The feeding strategy based on V-VST can reduce the incidence of complications, shorten the length of hospital stay and improve the swallowing function. [ABSTRACT FROM AUTHOR]
- Published
- 2020
5. 高等医学院校学生就读经验及影响因素.
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HAN Jing, ZHANG Xiaoping, LI Li, and TANG Juan
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- 2019
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6. Compilation and evaluation on core competency evaluation index system for aged caregiver.
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Han Jing, Tang Juan, and Zhang Xiaoping
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- 2017
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7. Influence of different turn over intervals on incidence of ICU inevitable pressure ulcers.
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Li Guihong and Tang Juan
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- 2017
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8. Research on automotive standard drive and protection circuit of IGBT.
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HUANG Lei and LI Tang-juan
- Abstract
In order to research the drive and protection circuit of insulated gate bipolar transistor (IGBT), a protection and drive scheme based on automotive standard IC 1ED020I12FTA was designed and implemented on the motor drive system. The protection principle, the gate drive circuit and the selection of gate drive resistance and the EMS design were explained in detail. Finally, a complete experimental system was built and tested. The experimental results show that the designed drive circuit has good switch performance. The motor drive system with this circuit has high reliability and stability and can output the power the motor needed. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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9. Isolation, identification and virulence analysis of Streptococcus suis.
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WANG Cai-xian, LIU Hua-dong, ZHAN Li-e, LU Bing-yang, TANG Juan, ZHAN Hai-jie, JING Ya-li, and LI Ting-ting
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- 2014
10. Immune experiment of co-expresstion F and HN protein of partridge chickens Newcastle disease virus strain SX-1.
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LI Hong-li, ZHAN Li-e, WANG Cai-xian, TANG Juan, LU Bing-yang, and DING Zhuang
- Published
- 2011
11. Clinical observation and nursing care of heat castrate package treatment for patients with heat stasis type pelvic inflammatory mass.
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Qin Xiaorun and Tang Juan
- Published
- 2013
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12. [Simultaneous determination of 10 quaternary ammonium salt bactericides in oral care products by high performance liquid chromatography-evaporative light-scattering detection].
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Tang J, Ding YC, Fei XQ, Wu B, Qian ZJ, Chen SD, and Li B
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- Chromatography, High Pressure Liquid, Anti-Bacterial Agents analysis, Light, Scattering, Radiation, Quaternary Ammonium Compounds chemistry, Quaternary Ammonium Compounds analysis
- Abstract
Quaternary ammonium salt bactericides are broad-spectrum bactericides often used in oral care products because of their high antibacterial efficacy, strong penetration, and low toxicity. However, the excessive use of quaternary ammonium salt bactericides may cause contact dermatitis, scalding poisoning, and even death. Existing methods to determine quaternary ammonium salt bactericides are unable to meet current requirements owing to the lack of determination components. Therefore, establishing a simple and accurate method for the simultaneous detection of more quaternary ammonium salt bactericides is necessary. In this study, a method that couples sample pretreatment with high performance liquid chromatography-evaporative light-scattering detection (HPLC-ELSD) was developed for the simultaneous determination of quaternary ammonium salt bactericides in oral care products, including dodecyltrimethylammonium chloride, dodecyldimethylbenzylammonium chloride, benzethonium chloride, tetradecyl trimethyl ammonium chloride, tetradecyldimethylbenzylammonium chloride, N -hexadecyltrimethylammonium chloride, benzyldimethylhexadecylammonium chloride, trimethylstearylammonium chloride, stearyldimethylbenzylammonium chloride, and docosyltrimethylammonium chloride. Some of these bactericides do not absorb ultraviolet light, so a universal evaporative light-scattering detector was used owing to testing cost and stability concerns. The paste samples contained thickening agents, which are highly soluble in water but insoluble in organic solvents; these agents can seriously affect the results of sample pretreatment and damage the chromatographic column. Hence, sample dehydration was necessary. In this study, four dehydration methods were compared. Anhydrous sodium sulfate (Na
2 SO4 ) was selected, and the amount of Na2 SO4 was optimized. Based on the solubility of the 10 target compounds and extraction efficiency, three extraction solvents were compared, and ethanol was selected. Ultrasonic extraction was the primary extraction process used in this study. The effects of different ultrasonication times, temperatures, and powers on the extraction recoveries were also investigated. Ultimately, the optimized conditions were as follows: extraction of the dehydrated paste and powder samples using ethanol at room temperature (25 ℃) for 20 min under 100 W ultrasound power, and dilution of the liquid sample with ethanol. After extraction, the samples were separated on an Acclaim Surfactant column (150 mm×4.6 mm, 5 μm) with 50 mmol/L ammonium acetate aqueous solution (pH=5.5) (A) and acetonitrile (B) as mobile phases. The gradient elution program were as follows: 0-5.0 min, 75%A-35%A, 5.0-15.0 min, 35%A-20%A, 15.0-20.0 min, 20%A, 20.0-21.0 min, 20%A-75%A, 21.0-25.0 min, 75%A. An external standard method was used for quantitative determination. The 10 compounds were analyzed within 25 min. Linear equations, correlation coefficients, and linear ranges were obtained by analyzing a series of mixed standard working solutions. The limits of detection (LODs, S/N =3) and quantification (LOQs, S/N =10) of the 10 components were determined. Stearyldimethylbenzylammonium chloride and docosyltrimethylammonium chloride showed good linear relationships in the range of 10-200 mg/L, while the other compounds demonstrated good linear relationships in the range of 5-100 mg/L. In all cases, correlation coefficients ( R2 ) of no less than 0.9992 were obtained. The LODs and LOQs were in the range of 1.42-3.31 mg/L and 4.25-9.94 mg/L, respectively. Ten analytes were spiked in blank matrices, such as toothpaste (paste), mouthwash (liquid), and dentifrice powder (powder) at three levels, and the recoveries and precisions were calculated. The average recoveries were 87.9%-103.1%, and the corresponding relative standard deviations (RSDs) did not exceed 5.5% ( n =6). The developed method was used to detect 109 oral care products. Benzyldimethylhexadecylammonium chloride and stearyldimethylbenzylammonium chloride revealed high detection rates. Moreover, the amount of stearyldimethylbenzylammonium chloride in one toothpaste sample exceeded regulatory requirements. Given its advantages of good precision and accuracy, the developed method is suitable for the quantitative analysis of the 10 aforementioned compounds in typical oral care products. The study findings can serve as a reference for the quality and safety monitoring of oral care products.- Published
- 2024
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13. [Risk factors for pancreatitis after endoscopic retrograde cholangiopancreatography in children].
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Yang L, Fu Y, Sun ZH, Zhou J, Tang J, and Ni J
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- Humans, Child, Male, Female, Risk Factors, Retrospective Studies, Child, Preschool, Adolescent, Logistic Models, Infant, Cholangiopancreatography, Endoscopic Retrograde adverse effects, Pancreatitis etiology, Pancreatitis prevention & control
- Abstract
Objectives: To investigate the application of endoscopic retrograde cholangiopancreatography (ERCP) in children and the risk factors for post-ERCP pancreatitis (PEP)., Methods: A retrospective analysis was conducted on the clinical data of 66 children, aged ≤16 years, who underwent ERCP for pancreaticobiliary diseases at the Gastrointestinal Endoscopy Center of the Second Affiliated Hospital of Kunming Medical University from September 2013 to September 2023. The incidence rate of PEP and the risk factors for the development of PEP were analyzed., Results: A total of 78 ERCP procedures were performed on 66 children, with 5 diagnostic ERCPs, 69 therapeutic ERCPs, and 4 failed procedures. The success rate of ERCP operations was 95% (74/78). There were 17 cases of PEP in total, with an incidence rate of 22%. In the PEP group, the proportion of children with normal preoperative bilirubin and the proportion of guidewire insertion into the pancreatic duct during surgery were higher than in the non-PEP group ( P <0.05). The multivariate logistic regression analysis showed that guidewire insertion into the pancreatic duct was an independent risk factor for PEP ( P <0.05)., Conclusions: With the increasing application of ERCP in children with pancreaticobiliary diseases, it is important to select an appropriate intubation technique during surgery to avoid blindly entering the guidewire into the pancreatic duct and reduce the occurrence of PEP.
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- 2024
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14. [Expert consensus on the diagnosis and treatment of respiratory diseases exacerbated by nonsteroidal anti-inflammatory drugs (2024, Chengdu)].
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Meng J, Ye J, Zhang L, Xu Y, Liu W, Yang Y, Wang X, Jiang Y, Qiu Q, Li J, Yu S, Liu F, Lou H, Wang H, Yu G, Xu R, Meng C, Sun N, Chen J, Zeng M, Xie Z, Sun Y, Tang J, Zhao K, Zhang W, Shi Z, Xu C, Yang Y, Lu M, Ye H, Wei X, Sun B, An Y, Sun Y, Gu Y, Zhang T, Ba L, Yang Q, Xu Y, Yang G, Hong H, Zuo K, and Li H
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- Humans, China, Rhinitis diagnosis, Rhinitis therapy, Rhinitis chemically induced, Sinusitis diagnosis, Sinusitis therapy, Sinusitis drug therapy, Consensus, Asthma diagnosis, Asthma drug therapy, Chronic Disease, Anti-Inflammatory Agents, Non-Steroidal adverse effects
- Abstract
Non-steroidal anti-inflammatory drugs-exacerbated respiratory disease (N-ERD) is a chronic respiratory disease characterized by eosinophilic inflammation, featuring chronic rhinosinusitis (CRS), asthma, and intolerance to cyclooxygenase 1 (COX-1) inhibitors. The use of these medications can lead to an acute worsening of rhinitis and asthma symptoms. This condition has not yet received sufficient attention in China, with a high rate of misdiagnosis and a lack of related research. The Chinese Rhinology Research Group convened a group of leading young experts in otolaryngology from across the country, based on the latest domestic and international evidence-based medical practices to formulate this consensus.The consensus covers the epidemiology, pathogenesis, clinical manifestations, diagnostic methods, and treatment strategies for N-ERD, including pharmacotherapy, surgery, biologic treatments, and desensitization therapy. The goal is to improve recognition of N-ERD, reduce misdiagnosis, and enhance treatment outcomes., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Editorial Department of Journal of Clinical Otorhinolaryngology Head and Neck Surgery.)
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- 2024
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15. [Neutrophil-lymphocyte and platelet-lymphocyte ratios for assessing disease activity in patients with rheumatoid arthritis receiving tofacitinib treatment].
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Tang J, Chen J, Lin G, Zhang H, Gui M, Li N, Gu Y, Luo L, and Sun J
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- Humans, Retrospective Studies, C-Reactive Protein analysis, Interleukin-6 metabolism, Lymphocytes, Neutrophils, Arthritis, Rheumatoid
- Abstract
Objective: To evaluate the value of neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) for assessing disease activity in patients with rheumatoid arthritis (RA) treated with tofacitinib., Methods: This retrospective study was conducted among 98 RA patients in active stage treated with tofacitinib in Third Xiangya Hospital and 100 healthy control subjects from the Health Management Center of the hospital from 2019 to 2021. We collected blood samples from all the participants for measurement of erythrocyte sedimentation rate (ESR), high-sensitivity C-reactive protein (hs-CRP), interleukin-6 (IL-6) and other blood parameters 1 month before and 6 months after tofacitinib treatment. We further evaluated PLR and NLR before and after tofacitinib treatment in the RA patients, and analyzed their correlations with RA disease activity., Results: PLR and NLR increased significantly in RA patients as compared with the healthy controls. In the RA patients, PLR and NLR were positively correlated with the levels of hs- CRP, ESR, IL- 6, Disease Activity Score of 28 joints-ESR (DAS28-ESR), anti-cyclic citrullinated peptide (CCP), and rheumatoid factor (RF) before and after tofacitinib treatment. Tofacitinib treatment for 6 months significantly decreased hs-CRP, ESR, IL-6, CCP, RF and DAS28-ESR levels in the RA patients., Conclusion: NLR and PLR can be useful biomarkers for assessing disease activity in RA patients treated with tofacitinib.
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- 2023
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16. [Potential pharmacodynamic substances of Laportea bulbifera in treatment of rheumatoid arthritis based on serum pharmacochemistry and pharmacology].
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Tang J, Zhang Q, Wu D, Chen SY, Chen Y, Li YT, Zheng L, Huang Y, Lan YY, Wang YL, and Gong ZP
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- Animals, Chlorogenic Acid analogs & derivatives, Cytokines metabolism, Dinoprostone, Humans, Interleukin-1beta genetics, Interleukin-6, Plant Extracts pharmacology, Plant Extracts therapeutic use, Quinic Acid analogs & derivatives, Rutin, Tumor Necrosis Factor-alpha genetics, Tumor Necrosis Factor-alpha metabolism, Arthritis, Experimental drug therapy, Arthritis, Rheumatoid drug therapy, Urticaceae chemistry
- Abstract
The present study investigated the pharmacodynamic material basis of Laportea bulbifera in the treatment of rheumatoid arthritis. Firstly, human rheumatoid arthritis fibroblast-like synoviocyte line MH7A was cultured in vitro and treated with tumor necrosis factor alpha(TNF-α, 50 ng·mL~(-1)). The proliferation and the levels of inflammatory cytokines such as prostaglandin E2(PGE2), interleukin-1β(IL-1β), and interleukin-6(IL-6) of the MH7A cells exposed to the serum containing L. bulbifera were determined to evaluate the anti-rheumatoid arthritis effects of the serum. Furthermore, the ultra-performance liquid chromatography tandem mass spectrometry fingerprints of the L. bulbifera crude extract, the drug-containing serum, and the drug-free serum were compared to identify the compounds newly generated in the serum after oral administration of the extract. According to the peak areas of common peaks and the results of anti-rheumatoid arthritis effect test, the active components were identified. The serum containing L. bulbifera significantly inhibited the proliferation of the MH7A cells activated by TNF-α and the expression of PGE2, IL-6, and IL-1β. Thirty newly generated compounds were detected in the drug-containing serum. Among them, neochlorogenic acid, cryptochlorogenic acid, chlorogenic acid, rutin, isoquercitrin, luteoloside, kaempferol-3-O-rutinoside, and quercitrin were also present in the crude extract. Twelve characteristic peaks(3, 7, 8, 14, 18, 19, 21, 23, 24, m6, m7, and m15) were significantly correlated with the pharmaceutical effect. According to the correlations, neochlorogenic acid, cryptochlorogenic acid, and chlorogenic acid had great contributions to the anti-rheumatoid arthritis activity. This study preliminarily clarified the potential pharmacodynamic substances of L. bulbifera in the treatment of rheumatoid arthritis, which laid a theoretical and experimental foundation for further development and application of the medicinal plant.
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- 2022
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17. [The application and evaluation of a new PBL-oriented open experimental teaching model in basic medical experiment course].
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Tang J, Jiang J, and Chen Z
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- Humans, Learning, Problem-Based Learning methods, Students, Medical
- Abstract
Objective To evaluate the effect of this new problem-based learning(PBL) oriented open experimental teaching model. Methods The new model of open experimental teaching was implemented in the medical cell biology experiment course of 2019 program in clinical medicine at Air Force Military Medical University. The results of teaching practice was evaluated by examination and questionnaires. Results The final test scores of the experimental group were significantly higher than those of the control group. Questionnaire survey of the experimental group students showed that the new model stimulated their learning interest and enthusiasm, improved their innovation and research capability, strengthened their abilities in literature review and problem solving. Conclusion This new PBL oriented open experimental teaching mode effectively improves students' academic performance, and cultivate their scientific spirit, innovative thinking and ability to solve scientific problems. The model evolves with modern medical development and cultivates innovative talents. It can be widely applied in the experiment teaching of basic medicine, such as immunology and biochemistry.
- Published
- 2022
18. [Determination of adsorbable organic halogens in textiles by ultrasonic extraction-high temperature combustion absorption-ion chromatography].
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Ding Y, Cao L, Zhou L, Qian K, Tang J, Zhou J, and Dong S
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- Animals, Chromatography, Ions, Temperature, Textiles, Halogens, Ultrasonics
- Abstract
Adsorbable organic halogens (AOX) are often introduced or produced in textile and dyeing processes, such as the chlorination shrink proof process of wool, the bleaching process by sodium hypochlorite and chlorite, the dry-cleaning process by chlorinated solvent, etc. However, part of AOX is difficult to biodegrade and is a persistent bioaccumulative toxic substance with high fat solubility. To promote clean production of textiles and to protect the health of consumers, a conventional method for the detection of AOX in textiles must be established urgently. In this study, a new method was developed for the determination of AOX in textiles by ultrasonic extraction-high temperature combustion absorption-ion chromatography (IC). In this method, AOX in textiles were extracted by ultrasonic extraction at room temperature with ultra-pure water as solvent. Activated carbon was added to the extraction solution for oscillatory adsorption and removal of inorganic halides with acidic sodium nitrate solution. The AOX adsorbed on activated carbon were cracked, burned, and gasified by the oxidative combustion method with a programmed heating mode. The product hydrogen halide gas entered the absorption solution with the carrier gas, followed by separation and determination by IC with external standard method of quantification. During the experiment, the pretreatment conditions were optimized, including the extraction time of AOX, the amount of activated carbon, the combustion gas and its flow rate, the temperature program for high-temperature oxidation combustion, the absorption method, and the absorption solution. The instrument conditions of ion chromatography, including the chromatographic column, column temperature, eluent, and its flow rate, were also optimized. The results showed that the method was linear in the range of 0.02-10 mg/L for the standard solutions of fluorine, chlorine, bromine, and iodine ions, and the correlation coefficients ( R
2 ) were greater than 0.999. The limits of quantification of the method for AOX were 0.10-0.50 mg/kg. The negative textile samples of cotton, wool, and polyester were used as the sample matrix, and typical organic halogens were selected for standard addition and recovery. At low, medium, and high spiked levels, the average recoveries of AOX in cotton, wool, and polyester fiber were 82.3%-98.7%. The corresponding relative standard deviations (RSDs, n =7) were 2.0%-5.7%, indicating that the method had good recovery and precision. This method was used to determine actual textile samples, and AOX at different contents were detected in blue coated polyester fabric and black modal fabric with good repeatability. The established method improved the recoveries of AOX converted into inorganic halogens via oscillating adsorption of activated carbon, high-temperature oxidation combustion with a programmed heating mode, and secondary absorption using a porous absorption bottle. Meanwhile, the separation and detection of halogen ions was successfully conducted using the ion chromatography instrument with good selectivity and high sensitivity, without any interference of impurity ions. The method is simple, accurate, and reliable, and fully meets the limit requirements of domestic and foreign regulations and textile standards; the method is also suitable for the detection and analysis of AOX in textiles.- Published
- 2022
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19. [Effects of Notch-1/Twist-1 axis in the process of epithelial-mesenchymal transition of type II alveolar epithelial cell and its mechanism].
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Tang J, Ke J, Fu Y, He XW, and Li XW
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- Animals, Bleomycin, Lung, Rats, Signal Transduction, Transforming Growth Factor beta1, Alveolar Epithelial Cells cytology, Epithelial-Mesenchymal Transition, Pulmonary Fibrosis chemically induced, Receptor, Notch1 metabolism, Twist-Related Protein 1 metabolism
- Abstract
Objective: To study the role of Notch-1/Twist-1 axis in the process of epithelial-mesenchymal transition (EMT) of type II alveolar epithelial cells in pulmonary fibrosis (PF) and hope to provide a new theoretical basis for the pathogenesis of PF. Methods: Thirty rats were randomly divided into control group and bleomycin (BLM) group, 15 rats in each group. The PF rat model was induced by intratracheal injection of BLM (7 500 U/kg). Excised inferior lobe of left lung was fixed in 10% formalin for HE staining, Masson staining and transforming growth factor-beta 1 (TGF-β1) immunohistochemistry staining after BLM injection for 28 days. The cultured type II alveolar epithelial cells (RLE-6TN) were divided into 4 groups (Control group, transforming growth factor-beta 1 (TGF-β1) group, Notch-1 negative control siRNA (NC siRNA, 100 pmol/L) group and Notch-1 siRNA (100 pmol/L) group), each group was established nine holes. The cells were treated with TGF-β1 (5.0 ng/ml) for 24 h following NC siRNA or Notch-1 siRNA for 48 h. The mRNA and (or) proteins levels of TGF-β1, collagen I, collagen III, E-Cadherin, zonula occludens-1 (ZO-1), Vimentin, E-Cadherin, Notch-1, Notch-1 intracellular domain (NICD), Hes-1 and Twist-1 were detected in lung tissue and type II alveolar epithelial cells. Results: In vivo , compared with the control group, the alveolar atrophy, collapse and fusion occurred, alveolar septum widened significantly, and a large number of inflammatory cells infiltrated in the pulmonary interstitial of the rats in the BLM group. And compared with control group, BLM obviously increased collagen deposition and collagen I and collagen III expressions, while the expressions of ZO-1 and E-cadherin were decreased, and the expressions of Vimentin and N-cadherin were increased, and concomitantly with increasing Notch-1, NICD, Hes-1 and Twist-1 expression in lung tissues of rats ( P <0.01). In vitro , compared with control group, TGF-β1 treatment obviously induced collagen I, collagen III, Notch-1, NICD, Hes-1 and Twist-1 expressions, and the expressions of E-cadherin and ZO-1 were decreased and the expressions of Vimentin and N-cadherin were increased( P <0.01). Compared with TGF-β1 group, Notch-1 siRNA treatment significantly inhibited the expressions of Notch-1, NICD, Hes-1 and Twist-1, and the expressions of E-cadherin and ZO-1 were increased and the expressions of Vimentin and N-cadherin were decreased, and also obviously reduced the expressions of collagen I and collagen III induced by TGF-β1 ( P <0.05 or P <0.01). Conclusion: Notch-1/Twist-1 axis is involved in the EMT process of type II alveolar epithelial cells, suggesting that Notch-1/Twist-1 signaling may be involved in the development of pulmonary fibrosis.
- Published
- 2021
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20. [Simultaneous determination of 18 chlorinated hydrocarbon organic solvents in cosmetics by gas chromatography-mass spectrometry].
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Tang J, Fei X, Zhou J, Qian K, Dong S, Cao L, and Ding Y
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- Gas Chromatography-Mass Spectrometry, Preservatives, Pharmaceutical, Cosmetics analysis, Hydrocarbons, Chlorinated analysis, Solvents analysis
- Abstract
Organic solvents can be used to dissolve and disperse flavors, bactericides, preservatives, surfactants, oils, and coloring agents during the production of cosmetics. However, harmful chlorinated hydrocarbon organic solvents are found in cosmetics such as manicure products, anti-acne products, and perfumes. Long-term contact with such cosmetics will have an adverse effect on the consumers' health. Past research has focused on very few chlorinated hydrocarbon organic solvents in cosmetics. Most organic solvents with low boiling points are typically determined by headspace-gas chromatography-mass spectrometry. In this study, a high-boiling-point solvent was used as the injection solvent, and the solvent delay time was cancelled. The compounds that could only peak during the solvent delay time were effectively separated. A method coupling sample pretreatment with gas chromatography-mass spectrometry (GC-MS) was developed for the simultaneous determination of 18 chlorinated hydrocarbon organic solvents in cosmetics: vinylidene chloride, dichloromethane, trans -1,2-dichloroethylene, 1,1-dichloroethane, cis -1,2-dichloroethylene, chloroform, 1,1,1-trichloroethane, carbon tetrachloride, 1,2-dichloroethane, trichloroethylene, 1,1,2-trichloroethane, tetrachloroethylene, 1,1,1,2-tetrachloroethane, 1,1,2,2-tetrachloroethane, 1,2,3-trichloropropane, pentachloroethane, hexachloroethane, and hexachloro-1,3-butadiene. These 18 solvents have a wide range of polarities and a notable difference in volatilities, in addition to many isomers and structural analogs, which renders their separation difficult. Therefore, the separation effect of three kinds of GC columns with different polarities was compared. n -Tetradecane, an injection solvent with good solubility, was selected as the extraction solvent. An organic solvent with high polarity has low extraction rate because of its weak polarity. Adding sodium chloride solution to the sample to induce the "salting out" effect could change the partition coefficient of the components, thereby improving the extraction rate. Therefore, the concentration of the sodium chloride solution added to the sample was optimized. In this work, liquid-liquid extraction was the main extraction process, so the effects of different shaking times, temperatures, and frequencies on the extraction rate were discussed. The optimized results are as follows: at normal temperature, the sample dispersed or dissolved in saturated sodium chloride solution was extracted by n -tetradecane at an oscillating speed of 100 r/min for 20 min. Separation was performed on an Agilent J&W DB-624 column (30 m×0.25 mm×1.4 μm) by GC-MS with an electrospray ionization (EI) source in the selected ion monitoring (SIM) mode. The external standard method was used for quantitative determination. The 18 compounds could be analyzed within 19 min. The linear equations, linear correlation coefficients, and linear ranges were obtained by analyzing a series of mixed standard working solutions. The limits of detection (LODs, S/N =3) and limits of quantification (LOQs, S/N =10) of the 18 components were determined. The negative lipstick (solid) and mouthwash (liquid) samples were used as the spiked sample matrix at three levels, and the recoveries and precisions were calculated. The calibration curves showed good linearities for the 18 chlorinated hydrocarbon organic solvents in range of 0.2-100 mg/L, with correlation coefficients ( R
2 ) not less than 0.9992. The LODs and LOQs were in the range of 0.033-0.049 mg/L and 0.10-0.15 mg/L, respectively. The average recoveries of the 18 chlorinated hydrocarbon organic solvents in lipstick (solid) and mouthwash (liquid) were 92.4%-103.1% and 93.3%-102.4% respectively; the corresponding relative standard deviations (RSDs) were 3.1%-5.3% and 2.8%-5.4% ( n =6). This method was used to determine 115 different types of cosmetics, and tetrachloroethylene was detected in three nail polishes. With its advantages of high sensitivity, good precision, and accuracy, the developed method is suitable for the quantitative analysis of the aforesaid 18 compounds in all kinds of cosmetics. The study findings would serve as a reference for the quality and safety monitoring of cosmetics.- Published
- 2021
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21. [Chrysin inhibited epithelial-mesenchymal transition of type Ⅱ alveolar epithelial cell by regulating NF-κB/Twist 1 signaling pathway].
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Lu LM, Fu Y, Li QY, He XW, Tang J, and Li XW
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- Alveolar Epithelial Cells metabolism, Animals, Flavonoids, Rats, Signal Transduction, Transforming Growth Factor beta1 genetics, Epithelial-Mesenchymal Transition, NF-kappa B genetics, NF-kappa B metabolism
- Abstract
This study aimed to assess whether chrysin(ChR) can inhibit epithelial-mesenchymal transition(EMT) of type Ⅱ alveolar epithelial cell and produce anti-pulmonary fibrosis effect by regulating the NF-κB/Twist 1 signaling pathway. Sixty rats were randomly divided into the control group, the bleomycin(BLC) group, BLC+ChR(50 mg·kg~(-1)) group and BLC+ChR(100 mg·kg~(-1)) group, with 15 rats in each group. The pulmonary fibrosis model was induced by intratracheal injection of BLC(7 500 U·kg~(-1)). Rats were orally administered with different doses of ChR after BLC injection for 28 days. The cells were divided into control group, TGF-β1 group(5 ng·mL~(-1)), and TGF-β1+ChR(1, 10, 100 μmol·L~(-1)) groups. The type Ⅱ alveolar epithelial cells were treated with TGF-β1 for 24 h, and then treated with TGF-β1 for 48 h in the presence or absence of different doses of ChR(1, 10 and 100 μmol·L~(-1)). The morphological changes and collagen deposition in lung tissues were analyzed by HE staining, Masson staining and immunohistochemistry. The mRNA and protein expression levels of collagen Ⅰ, E-cadherin, zonula occludens-1(ZO-1), vimentin, alpha smooth muscle actin(α-SMA), inhibitor of nuclear factor kappa B alpha(IκBα), nuclear factor-kappa B p65(NF-κB p65), phospho-NF-κB p65(p-p65) and Twist 1 in lung tissues and cells were detected by qPCR and Western blot, respectively. The animal experiment results showed that as compared with the BLC group, after administration of ChR for 28 days, bleomycin-induced pulmonary fibrosis in rats was significantly relieved, collagen Ⅰ expression in lung tissues was significantly reduced(P<0.05 or P<0.01), and EMT of alveolar epithelial cells was obviously inhibited [the expression levels of E-cadherin and ZO-1 were increased and the expression levels of vimentin and α-SMA were decreased(P<0.05 or P<0.01)], concomitantly with significantly reduced IκBα and p65 phosphorylation level in cytoplasm and decreased NF-κB p65 and Twist 1 expression in nucleus(P<0.05 or P<0.01). The cell experiment results showed that different doses of ChR(1, 10 and 100 μmol·L~(-1)) significantly reduced TGF-β1-induced collagen Ⅰ expression(P<0.05 or P<0.01), significantly inhibited EMT of type Ⅱ alveolar epithelial cells[the expression levels of E-cadherin and ZO-1 were increased and the expression levels of vimentin and α-SMA were decreased(P<0.05 or P<0.01)], and inhibited IκBα and p65 phosphorylation in cytoplasm and down-regulated NF-κB p65 and Twist 1 expression in nucleus induced by TGF-β1(P<0.05 or P<0.01). The results suggest that ChR can reverse EMT of type Ⅱ alveolar epithelial cell and alleviate pulmonary fibrosis in rats, and its mechanism may be associated with reducing IκBα phosphorylation and inhibiting NF-κB p65 phosphorylation and nuclear transfer, thus down-regulating Twist 1 expression.
- Published
- 2021
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22. [Study on metabolites of Laportea bulbifera extract in rat feces based on UHPLC-Q-TOF-MS~E technique].
- Author
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Wu D, Li Y, Chen SY, Tang J, Li YT, Pan J, Huang Y, Zheng L, Wang AM, Li YJ, Lan YY, and Gong ZP
- Subjects
- Administration, Oral, Animals, Chromatography, High Pressure Liquid, Feces, Plant Extracts, Rats, Rats, Sprague-Dawley, Urticaceae
- Abstract
This project is to study the metabolites of Laportea bulbifera extract in rat feces. After the SD rats were gavaged with the extract(136 g·kg~(-1), according to the crude drug dose), the metabolites in their feces were detected by UHPLC-Q-TOF-MS~E technique, and the obtained mass spectrometry data was combined with UNIFI software for prediction. The prototype components and metabolites in rat feces were identified with reference materials and related literature. A total of 43 metabolites were identified(including 8 prototype components and 35 metabolites). The metabolic pathways mainly include monocaffeoylquinic acid(hydrogenation reduction, ring-opening cracking, sulfation, hydroxylation, glucuronidation), quercetin(O-C2 bond ring-opening cleavage, C2-C3 double bond reduction, rutin carbonylation) and so on. The metabolites and metabolic process of L. bulbifera extract in rat feces were clarified, which provided a basis for the study of the active substances and its mechanism of action.
- Published
- 2020
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23. [Effect of small dose capsaicin for treatment of pulmonary fibrosis in mice and its mechanism].
- Author
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Lu LM, Yu TT, He XW, Tang J, and Li XW
- Subjects
- Animals, Antipruritics pharmacology, Antipruritics therapeutic use, Bleomycin toxicity, Disease Models, Animal, Lung pathology, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Random Allocation, Transforming Growth Factor beta1, Alveolar Epithelial Cells drug effects, Capsaicin administration & dosage, Capsaicin pharmacology, Epithelial-Mesenchymal Transition drug effects, Pulmonary Fibrosis chemically induced, Pulmonary Fibrosis drug therapy
- Abstract
Objective: To observe whether the mechanism of small dose capsaicin (Cap) against pulmonary fibrosis in mouse is mediated by agitating transient receptor potential vanilloid 1 (TRPV1). Methods: A total of 60 BALB/c mice were randomly divided into control (CON) group, bleomycin (BLM)group, Cap (0.5, 1,2 mg/kg) groups and Cap (2 mg/kg) plus SB-452533 (2.5 mg/kg) group. C57BL/6 mice were intratracheally injected with 3.5 mg/kg BLM to induce pulmonary fibrosis model. Animals for drugs treatment received daily drug via subcutaneous injection for 21 days. The morphological changes and collagen deposition in lung tissues were analysed by HE staining, Masson staining and immunohistochemistry. The concentration of calcitonin gene-related peptide (CGRP) in plasma was determined by ELISA. The mRNA and (or) proteins levels of α-CGRP, β-CGRP, collagen I, collagen III, E-Cadherin, zonula occludens-1 (ZO-1), vimentin, alpha smooth muscle actin (α-SMA), TRPV1, p-ERK1/2 and eukaryotic initiation factor 3a (eIF3a) were detected by qPCR and (or) Western blot. Results: Compared with the BLM group, small dose Cap significantly reduced bleomycin-induced pulmonary fibrosis in mice and obviously reversed alveolar epithelial cells epithelial-mesenchymal transition (EMT) (the expression of E-cadherin and ZO-1 were increased(P<0.05 or P<0.01)and the expression of α-SMA and Vimentin were decreased (P<0.05 or P<0.01) after drugs treatment for 21 day, concomitantly with the increase the expressions of TRPV1 and CGRP (P<0.05 or P<0.01), and inhibiting ERK1/2 phosphorylation and eIF3a expression (P<0.05 or P<0.01). These effects of small dose Cap were abolished in the presence of TRPV1 receptor antagonist SB-452533. Conclusion: The results suggest that small dose Cap can reverse alveolar epithelial cells EMT and alleviate bleomycin-induced pulmonary fibrosis in mice by inhibiting ERK1/2/eIF3asignaling pathway, which is related to agitating TRPV1 receptor and releasing of CGRP.
- Published
- 2020
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24. [Differences in intestinal absorption characteristics of Laportea bulbifera extract in normal and rheumatoid arthritis model rats by isolated everted intestine model].
- Author
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Li Y, Chen SY, Gong ZP, Kang NF, Wu D, Tang J, Li YT, Pan J, Huang Y, Zheng L, Lan YY, Li YJ, and Wang YL
- Subjects
- Animals, Chromatography, High Pressure Liquid, Intestines drug effects, Rats, Tandem Mass Spectrometry, Arthritis, Rheumatoid drug therapy, Intestinal Absorption, Plant Extracts therapeutic use, Urticaceae chemistry
- Abstract
This work aimed to investigate the intestinal absorption characteristics of Laportea bulbifera extract in normal and rheumatoid arthritis model rats. The contents of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, rutin, kaempferol-3-O-rutinoside, galuteolin, quercetin and isoquercetin in intestinal absorption solution samples were detected by UPLC-MS/MS with 5.0 g·L~(-1) as the absorption concentration. The cumulative absorption(Q) and absorption rate constant(K_a) were calculated, and the absorption characteristics of different components of L. bulbifera in intestinal absorption solution of normal rats and rheumatoid arthritis rats were compared. The results showed that all the eight index components in the extract of L. bulbifera could be absorbed into the intestinal capsule, the cumulative absorption-time curve of each component showed an upward trend without saturation, and the correlation regression coefficient(R~2) was greater than 0.92, which is consistent with the zero-order absorption rate process. It was speculated that the possible absorption mode of each component was passive diffusion. In normal condition, the absorption of ileum was the best(except chlorogenic acid), and in pathological condition, duodenum was the best. The total absorption of 8 components in each intestinal segment of RA rats was better than that of normal rats, which speculated that rheumatoid arthritis may change the specific site of drug absorption. The experimental results showed that rheumatoid arthritis could change the intestinal absorption of the extract of L. bulbifera, and its mechanism needs further study.
- Published
- 2020
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25. [Construction of rat interleukin-10 adenoviral vector and its expression in bone marrow mesenchymal stem cells].
- Author
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Zhang WT, Tang J, Zhao HM, and You JY
- Subjects
- Adenoviridae, Animals, Bone Marrow Cells, Genetic Vectors, HEK293 Cells, Humans, Interleukin-10, Rats, Transfection, Mesenchymal Stem Cells
- Abstract
Objective: To construct the recombinant adenoviral vector carrying the rat interleukin-10 (rIL-10) gene, and to investigate whether it is stably expressed in bone marrow mesenchymal stem cells., Methods: The rIL-10 gene was amplified by PCR from template rIL-10 cDNA, and the recovered 656 bp rIL-10 DNA fragment was cloned into pcDNA3.1 to construct pcDNA3.1-IL-10. Then HEK293 cells were transfected with pcDNA3.1-IL-10 and adenoviral vector for homologous recombination, and sequencing and PCR were used to evaluate whether recombination was successful. HEK293 cells were lysed by repeated freeze-thaw cycles, and bone marrow mesenchymal stem cells were infected with the virus solution containing the rIL-10 gene. Western blot was used to measure the expression of rIL-10 in bone marrow mesenchymal stem cells., Results: Sequencing and PCR verified that the rIL-10 adenoviral vector was successfully constructed, with a virus titer of 4×10
9 PFU/mL. The expression of IL-10 was detected after bone marrow mesenchymal stem cells were infected by the virus solution containing the rIL-10 gene., Conclusions: The constructed rIL-10 recombinant adenovirus can mediate the stable expression of rIL-10 gene in bone marrow mesenchymal stem cells, which provides a basis for gene transplantation therapy of inflammatory bowel disease.- Published
- 2019
26. [Interventional effect of epalrestat on renal interstitial fibrosis in unilateral ureteral obstruction rats and its mechanism].
- Author
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Gao YX, Tang J, Zhang Q, Jiang LL, and Li XW
- Subjects
- Animals, Fibrosis complications, Fibrosis drug therapy, Random Allocation, Rats, Rats, Sprague-Dawley, Rhodanine pharmacology, Enzyme Inhibitors pharmacology, Rhodanine analogs & derivatives, Thiazolidines pharmacology, Ureteral Obstruction complications, Ureteral Obstruction drug therapy
- Abstract
Objective: To observe the protective effects of epalrestat (EPS) on interstitial fibrosis in unilateral ureteral obstruction (UUO) rats and its mechanism., Methods: Rats were randomly divided into four groups: sham group, UUO group, UUO + epalrestat (50 or 100 mg/kg), 8 rats in each group.Rats in UUO and UUO + epalrestat group were obstructed left ureter.In the sham group, rats had their left ureters exposed and manipulated without ligation.Animals for epalrestat treatment received daily drug via gavage for 3 weeks, the rats of sham and UUO groups received equal amount of sodium carboxymethyl cellulose with the same regimen.Renal tissues pathological changes and collagen deposition were observed by HE and Masson's staining.The aldose reductase(AR) expression in renal tissues was measured by immunohistochemisty.The expression levels of collagen I, collagen III, alpha-smooth muscle actin (α-SMA), fibroblast-specific protein1 (FSP-1), fibronectin (FN), epithelial cadherin (E-cadherin), transforming growth factor-β1 (TGF-β1) and AR from kidney tissues were measured by real-time RT-PCR or Western blot., Results: Compared with the sham group, the renal tissues of the UUO group showed significant fibrosis, including renal tubular epithelial cell atrophy and vacuolated degeneration, collagen deposition, fibroblasts and myofibroblasts proliferation and inflammatory cell infiltration, and concomitantly with the expressions of collagen I, collagen III, TGF-β1, AR, α-SMA, FSP-1 and FN were remarkably up-regulated, but E-cadherin was significantly reduced in UUO group.Compared with the UUO group, after 3 weeks epalrestat administration, the level of renal interstitial fibrosis was obviously ameliorated and the expressions of collagen I, collagen III, TGF-β1, AR, α-SMA, FSP-1 and FN were remarkably down-regulated, but E-cadherin was significantly increased in rats of epalrestat groups., Conclusion: These results suggest that epalrestat attenuates renal interstitial fibrosis possibly through inhibition of EMT via inhibiting TGF-β1 and AR expression.
- Published
- 2019
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27. [Simultaneous determination of 18 fluorescent whitening agents in textiles by ultra performance liquid chromatography].
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Tang J, Zhou J, Qian K, Ding Y, Cheng Y, and Qi Y
- Abstract
A method was developed for the simultaneous determination of 18 fluorescent white-ning agents (FWAs) in textiles by ultra performance liquid chromatography with fluorescence detection (UPLC-FLR). The sample was extracted with chloroform-ethanol (6:4, v/v). The separation was performed on an ACQUITY UPLC HSS T3 column (100 mm×2.1 mm, 1.8 μm) with gradient elution. Methanol-water (containing 5 mmol/L ammonium acetate) was used as the mobile phase with a flow rate of 0.4 mL/min. The fluorescence detector was used at 350 nm excitation wavelength and 430 nm emission wavelength. External standard method was used for the quantitative determination. The calibration curves showed good linearity in respective ranges for the 18 FWAs with correlation coefficients ( R
2 ) no less than 0.9992. The limits of quantifications (LOQs, S/N =10) were in the range of 0.002-0.1 mg/L. The average recoveries of the 18 FWAs ranged from 88.3% to 104.5% at the different spiked levels with the relative standard deviations (RSDs) of 2.0%-5.5% ( n =6). The method has high sensitivity, good precision and accuracy, and is applicable to the determination of all kinds of textiles.- Published
- 2018
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28. [Implementation of enhanced recovery after surgery in nutritional management of patients undergoing metabolic surgery].
- Author
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Zhang T, Xu Q, Yang N, Tang J, and Liang H
- Subjects
- Digestive System Surgical Procedures, Humans, Nutrition Therapy methods, Perioperative Care methods, Bariatric Surgery, Nutrition Therapy standards, Perioperative Care standards
- Abstract
Metabolic surgery is a gastrointestinal surgical procedure to treat obesity and its related co-morbidities with rapid development in recent years. Patients undergoing metabolic surgery have preoperative nutritional disorders, and the nutrition management for these patients is the key point of perioperative management. During the perioperative period, current research has preliminarily confirmed that perioperative managements including supplementation of micronutrients, preoperative evaluation of the weight loss, preoperative fasting and carbohydrate oral intake based on the full application of ERAS and characteristics of the patients undergoing metabolic surgery, are safe and effective in clinical practice. As for the postoperative diet strategy, current literature remains non-unified to identify the duration and the content of the nutrition managements. Domestic clinical reports about the postoperative nutrition managements after metabolic surgery are rare and lack of unified and good reference standard. Meanwhile, divergence still existed in current literature regarding to the content of the postoperative nutrition managements. Therefore, it is necessary to develop the standardized protocol for nutrition managements which is offering basis and reference for the clinical application of perioperative nutrition managements after metabolic surgery.
- Published
- 2017
29. [Analysis of self-management education on improving the symptoms of female college students with primary dysmenorrhea in Shaoyang].
- Author
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Tang J and Xu H
- Subjects
- Disease Management, Female, Humans, Quality of Life, Students, Surveys and Questionnaires, Universities, Dysmenorrhea therapy, Patient Education as Topic, Self Care
- Abstract
Objective: To explore the effect of self-management education on improving symptoms of female college students with primary dysmenorrhea and the health-related quality of life. , Methods: Female students with primary dysmenorrhea were chosen for the questionnaire in two colleges at Shaoyang. The female students were assigned to an interventional group and a control group. The interventional group (n=195) received self-management education for 6 months, while the control group (n=196) did not receive any intervention. We compared the symptoms scores, VAS, dysmenorrheal degree and health-related quality of life between the 2 groups before and after the intervention. , Results: After the intervention, the scores of symptoms, VAS and degree of dysmenorrhea were significantly lower than the baseline and the control group (P<0.05), and the physical component summary (PCS), mental component summary (MCS) were significantly improved (P<0.05). , Conclusion: Self-management education could effectively improve the symptoms of primary dysmenorrhea and quality of life.
- Published
- 2016
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30. [Benign notochordal cell tumor of cervical vertebrae: a clinicopathologic analysis].
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Huang J, Jiang Z, Tang J, and Zhang H
- Subjects
- Cervical Vertebrae diagnostic imaging, Chordoma pathology, Diagnosis, Differential, Diagnostic Imaging, Female, Humans, Male, Middle Aged, Notochord diagnostic imaging, Radiography, Retrospective Studies, Spinal Neoplasms diagnostic imaging, Cervical Vertebrae pathology, Notochord pathology, Spinal Neoplasms pathology
- Abstract
Objective: To report 2 rare cases of benign notochordal cell tumor (BNCT), according to WHO classification of tumors of soft tissue and bone (4th edition). Their radiologic and clincopathologic features and differential diagnosis were investigated., Methods: Two cases of BNCT were studied by retrospective review of the clinical, radiologic, pathologic and immunophenotypical findings. Related literatures were reviewed at the same time., Results: Case 1 was a 53-year-old man, and case 2 was a 61-year-old woman. Radiographically, both patients presented with abnormal imaging findings in the fifth cervical vertebral body with the lesions located within the bone but without extra osseous mass. Histopathologically, the lesions lacked lobular architecture and extracellular myxoid matrix. The tumor cells were vacuolated and had centrally or peripherally placed round or oval nuclei with small nucleoli, mimicking mature adipocytes. No cytological atypia or mitotic figures were seen. The affected bone trabeculae were sclerotic and islands of bone marrow were often entrapped within the tumor., Conclusions: Although sharing similar anatomic distribution and immunophenotype to those of chordoma, BNCT has distinct radiologic and pathologic features and different treatment and prognosis. The differential diagnosis between BNCT and chordoma requires detailed clinical, radiologic and histopathologic evaluations.
- Published
- 2014
31. [Rapid detection of eight fluorescent whitening agents in textile by ultra performance convergence chromatography].
- Author
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Tang J, Ding Y, Cao X, Qi Y, and Qian K
- Abstract
An accurate quantitative and confirmative method has been developed for the deter- mination of eight fluorescent whitening agents (FWAs) in textile by ultra performance conver- gence chromatography (UPC2) coupled with photo diode array (PDA) detection, including 1,2-bis (5-methyl-2-benzoxazole) ethylene (PF), 7-diethylamino-4-methylcoumarin (SWN), 2, 2'-(2,5-thiophenediyl) bis(5-(1,1-dimethylethyl)-benzoxazol (OB), 2-[4-[2-[4-(2-benzox- azolyl) phenyl] ethenyl] phenyl] -5-methyl-benzoxazol (KSN), 1,4-bis (2-cyanostyryl) benzene (ER-I), 1-(2-cyanostyryl)-4-(4-cyanostyryl) benzene (ER-II), 2,2'-(1,4-naphthalenediyl) bis-benzoxazol (KCB), 4,4'-bis[2-(2-methoxyphenyl) ethenyl]-1,1'-biphenyl (FP). The sample was extracted with xylene and concentrated by a rotary evaporator, and then qualitatively and quantitatively analyzed by UPC2. The separation of target compounds was achieved on an ACQUITY UPC2 HSS C18 SB column (100 mm x 3.0 mm, 1.8 μm) by a gradient elution with supercritical carbon dioxide and methanol as mobile phases. External standard method was used for the quantitative determination and the calibration curves showed good linearity in the concentration range of 1.0-20.0 mg/L for the eight target compounds with correlation coefficients not less than 0.999 1. The limits of quantification of the eight compounds (LOQs, S/N = 10) were 0.70-0.95 mg/L. The average recoveries of the eight compounds ranged from 90.9% to 96.5% at the spiked levels of 2.0, 5.0, 10.0 mg/kg with the relative standard deviations (RSDs) of 2.8%-4.2%. The method is simple, accurate and time-saving with high sensitivity, and can be used for the rapid determination of the eight FWAs in textile.
- Published
- 2014
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32. [Expression of Toll-like receptors 2 and 6 in mice liver during Schistosoma japonicum infection].
- Author
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Tang JJ, Ji XF, Zhu XM, Huang HY, and Li Z
- Subjects
- Animals, Cercaria, Mice, Mice, Inbred BALB C, Praziquantel, RNA, Messenger, Gene Expression Regulation, Liver, Schistosomiasis japonica metabolism, Toll-Like Receptor 2 genetics, Toll-Like Receptor 6 genetics
- Abstract
Objective: To investigate the expression of hepatic Toll-like receptor 1 (TLR1), TLR2 and TLR6 on mice with Schistosoma japonicum infection., Methods: Fifty BALB/c mice were infected with 20 +/- 3 S. japonicum cercariae through abdominal skin. At 6 weeks post-infection, the mice (n = 10) in treatment group were administered intragastrically with praziquantel [250 microg/(g x d)] for 3 d. The livers of mice (n = 10) were collected at pre-infection and 5, 6, 8 and 12 weeks post-infection, and then the mRNA expression levels of hepatic TLR1, TLR2, TLR6 gene were detected with reverse transfer PCR. Hepatic TLR2, TLR6 protein levels were detected by immunohistochemical staining., Results: The mRNA levels of TLR1, TLR2, and TLR6 on 5, 6, 8 and 12 weeks post infection were significantly higher than that of uninfected mice. After praziquantel treatment, the mRNA level of TLR2 and TLR6 in murine liver of treatment group was lower than that of infection group, but the level of TLR1 mRNA had no obvious change. Furthermore, immunohistochemistry results revealed that the expression of TLR2 and TLR6 proteins in murine liver was up-regulated at 5, 6, 8 and 12 weeks post-infection. After praziquantel treatment, the percentage of TLR2 positive area in liver of infected mice without and with praziquantel treatment were (44.2 +/- 4.3)%, (8.8 +/- 3.1)%, respectively, and TLR2 protein level was considerably down-regulated (P < 0.01). The percentage of TLR6 positive area in liver of infected mice without and with praziquantel treatment was (48.4 +/- 5.4)%, (37.4 +/- 3.5)%, respectively, and TLR6 level decreased slightly (P < 0.05)., Conclusion: The expression level of TRL2 and TLR6 in murine liver increases after Schistosoma japonicum infection. While compared with TLR2, the role of TLR6 in this progress is a weaker one.
- Published
- 2014
33. [Clinical effect of triple therapy combined with Saccharomyces boulardii in the treatment of Helicobacter pylori infection in children].
- Author
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Zhao HM, Ou-Yang HJ, Duan BP, Xu B, Chen ZY, Tang J, and You JY
- Subjects
- Amoxicillin administration & dosage, Child, Child, Preschool, Clarithromycin administration & dosage, Combined Modality Therapy, Drug Therapy, Combination, Female, Humans, Male, Omeprazole administration & dosage, Prospective Studies, Helicobacter Infections therapy, Helicobacter pylori, Probiotics administration & dosage, Saccharomyces
- Abstract
Objective: To evaluate the clinical effect of proton pump inhibitor-based triple therapy combined with Saccharomyces boulardii in the treatment of Helicobacter pylori (Hp) infection among children in terms of Hp eradication rate and incidence of adverse events., Methods: A prospective randomised controlled study was conducted on 240 children with a confirmed diagnosis of Hp infection. These patients were randomized into triple therapy (n=120) and probiotics groups (n=120). The triple therapy group received amoxicillin [40 mg/(kg·d), Tid], clarithromycin [15 mg/(kg·d), Bid] and omeprazole [0.7-0.8 mg/(kg·d), Qd], while the probiotics group received Saccharomyces boulardii (250 mg, Bid) in addition to triple therapy. The course of treatment was 14 days in both groups. The adverse events in subjects were recorded by their parents during treatment. Hp eradiation was evaluated by (13)C breath test at 4 weeks after treatment, and the eradication rate and incidence of adverse events were compared between the two groups., Results: The Hp eradication rates were 75.8% (91/120) in the triple therapy group and 85% (102/120) in the probiotics group (P>0.05). Compared with the triple therapy group, the probiotics group had nonsignificantly lower incidence of nausea, vomiting, and abdominal pain (P>0.05) and significantly lower incidence of stomatitis, constipation and diarrhea (P<0.05)., Conclusions: Triple therapy combined with Saccharomyces boulardii cannot significantly increase Hp eradication rate, but can significantly reduce the incidence of stomatitis, constipation, and diarrhea during treatment.
- Published
- 2014
34. [Clinical features and treatment of acute clenbuterol poisoning in children].
- Author
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Ou-Yang WX, Zhu YM, Lu XL, Yu SJ, Ding CZ, Ding YF, Liu FR, and Tang J
- Subjects
- Acute Disease, Adolescent, Child, Child, Preschool, Electrocardiography, Female, Humans, Infant, Male, Retrospective Studies, Adrenergic beta-Agonists poisoning, Clenbuterol poisoning
- Abstract
Objective: To study clinical features, treatment and curative effects in children with acute clenbuterol poisoning, in order to provide a basis for early diagnosis and treatment., Methods: Clinical data of 28 hospitalized children with acute clenbuterol poisoning in April 2011 were retrospectively studied., Results: Of the 28 patients, there were 15 males and 13 females, aged 1 to 13 years (mean age 6.5±4.8 years). Vomiting, palpitations and limb shaking were found as main clinical manifestations in the patients. Main changes of blood biochemical included hypokalemia, lactic acidosis, hyperglycemia, hypsocreatinkinase. Snus tachycardia and S-T segment depression were observed on ECG. Patients' symptoms were gradually alleviated after 12-78 hours by use of beta blockers, potassium supplement, protecting the heart and other symptomatic and supportive treatment. Blood biochemical indexes were improved after 48 hours of admission. All of the patients were cured after 5 days. The symptoms of the patients do not longer occur during a follow up of half a month., Conclusions: Acute clenbuterol poisoning is characterized by vomiting, palpitations, limb shaking, hypokalemia, lactic acidosis and tachycardia in children. An early effective treatment of this disease can improve prognosis in children.
- Published
- 2013
35. [Preliminary study of the prohibitin protein and paclitaxel resistance in ovarian cancer].
- Author
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Tang J, Cao L, Yi H, and Tang C
- Subjects
- Antineoplastic Agents, Phytogenic pharmacology, Female, Humans, Ovarian Neoplasms genetics, Ovarian Neoplasms pathology, Prohibitins, RNA Interference, RNA, Messenger genetics, RNA, Messenger metabolism, RNA, Small Interfering genetics, Repressor Proteins genetics, Transfection, Tumor Cells, Cultured, Drug Resistance, Neoplasm genetics, Ovarian Neoplasms metabolism, Paclitaxel pharmacology, Repressor Proteins metabolism
- Abstract
Objective: To determine the effect of RNA interference with transferred pshRNA/PHB on the biological characteristics of paclitaxel-resistant ovarian cancer cell lines., Methods: Western blot and real time-PCR were used to assay the expression of PHB protein and mRNA in SKOV3/Taxol-25 and SKOV3 cell lines. The SKOV3/Taxol-25 cell lines were transiently transfected by 3 target-specific small hairpin RNA (shRNA) interference fragments with fluorescent protein named the pshRNA427/PHB1, pshRNA248/PHB2, and pshRNA136/PHB3. The empty plasmid transfection via vehicle Lipofectamine2000 served as a negative control. The expression levels of PHB protein and mRNA were detected by Western blot and real time-PCR after the transfection for 48 h. The silence effect of PHB1 and PHB3 groups was obvious. PHB1, PHB3, and the negative control groups were used for the following experiments. MTT and flow cytometry assay were used to test the cell proliferation, IC50 of paclitaxel, and cell apoptosis in the 3 groups., Results: The expression levels of PHB protein and mRNA (2(-ΔΔCt)) were significantly higher in SKOV3/Taxol-25 cell line than those in SKOV3 cell line (P<0.05). The expression levels of PHB protein and mRNA were significantly lower in the PHB1 and PHB3 groups than those in the negative control group (P<0.05). The cell proliferations in the PHB1 and PHB3 groups were obviously slower than those in the negative control group after transfection for 48 h and 72 h (P<0.05). The IC50 of paclitaxel in the PHB1 and PHB3 groups significantly decreased after transfection for 72 h compared with the negative control group(P<0.05). The cell apoptotic rate in the PHB1 and PHB3 groups significantly increased after transfection for 48 h compared with the negative control group (P<0.05)., Conclusion: The shRNA/PHB can effectively suppress the expression of PHB gene in paclitaxel-resistant ovarian cancer cell lines. The cell proliferation in paclitaxel-resistant cell lines with removed PHB gene is significantly reduced. The apoptotic rate and the paclitaxel sensitivity of resistant cell lines with removed PHB gene are significantly increased. PHB gene is related to paclitaxel-resistance and interfering PHB gene expression may reduce paclitaxel resistance in ovarian cancer.
- Published
- 2012
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36. [Response of isozyme and stress indexes of Coptis chinensis to UV-B radiation].
- Author
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Wen Q, Zhang N, Cao RX, Zhou XY, Tang J, and Wu NB
- Subjects
- Electrophoresis, Polyacrylamide Gel, Oxidative Stress, Plant Leaves enzymology, Plant Leaves radiation effects, Plant Proteins metabolism, Plants, Medicinal enzymology, Plants, Medicinal radiation effects, Proline metabolism, Time Factors, Coptis enzymology, Coptis radiation effects, Isoenzymes metabolism, Malondialdehyde metabolism, Ultraviolet Rays
- Abstract
Objective: To study the physiological mechanism of anti-stress of Coptic chinensis and provide theoretical basis for its cultivation and promoting its quality., Methods: Different degrees of the range of time and intensity of UV-B radiation were set in the experiment. Used the technique of polyacrylamide gelatin vertical board electrophoresis (PAGE) to analyse the isozyme and related stress index., Results: The isoenzymic bands of SOD1 (Rf = 0.125), SOD2 (Rf = 0.312), CAT1 (Rf = 0.428), POD3 (Rf = 0.290), POD4 (Rf = 0.636) were induced by UV-B radiation after 3 hours, with the increase of the time of UV-B radiation, those isoenzymic bands was going to vanish or became unclear. Moreover, isoenzymic bands of CAT1 (Rf = 0.428), POD3 (Rf = 0.290) disappeared in advance under heavy intensity of UV-B radiation. Furthermore, the contents of MDA, soluble sugar, proline were higher dramatically than those of control group under UV-B radiation. However, excluding the increases of proline in UL group, the content of MDA, soluble sugar, proline of other groups commenced to decrease slowly and isoenzymic bands of soluble protein increase after 7 hours of UV-B radiation., Conclusion: The increase of the expression of antioxidase isozyme, accumulation of soluble sugar, soluble protein and other antioxidase matter is induced by the short-time UV-B radiation, which can protect Coptis chinensis from being harmed by UV-B radiation. However, regulation system of Coptis chinensis are broken, metabolism is disordered, the bands of antioxidase isozyme vanish or weaken, the bands of soluble protein are increased and widened, these phenomenon is caused by 7 hours of UV-B radiation.
- Published
- 2012
37. [Clinicopathologic features of osteochondroma with malignant transformation].
- Author
-
Zheng L, Zhang HZ, Huang J, Tang J, Liu L, and Jiang ZM
- Subjects
- Adult, Aged, Bone Neoplasms diagnostic imaging, Bone Neoplasms metabolism, Bone Neoplasms surgery, Chondrosarcoma diagnostic imaging, Chondrosarcoma metabolism, Chondrosarcoma surgery, Exostoses, Multiple Hereditary diagnostic imaging, Exostoses, Multiple Hereditary metabolism, Exostoses, Multiple Hereditary surgery, Female, Humans, Male, Middle Aged, Osteochondroma diagnostic imaging, Osteochondroma metabolism, Osteochondroma surgery, Radiography, S100 Proteins metabolism, Tumor Suppressor Protein p53 metabolism, Vimentin metabolism, Young Adult, Bone Neoplasms pathology, Cell Transformation, Neoplastic pathology, Chondrosarcoma pathology, Exostoses, Multiple Hereditary pathology, Osteochondroma pathology
- Abstract
Objective: To investigate the clinicopathologic, radiological and immunohistochemical characteristics of osteochondroma with malignant transformation., Methods: The clinical data, radiological imagings and hematoxylineosin stained histologic sections were reviewed in 463 cases of osteochondroma diagnosed in Shanghai 6th Hospital from 1991 to 2008, including 11 cases with malignant transformation. Immunohistochemical two-step staining was used to detect CK, vimentin, S-100 protein, p53 and c-myc expression in seven cases of osteochondroma with malignant transformation and 10 cases without malignant transformation. The relevant literature was reviewed., Results: Among the 11 cases with malignant transformation, five were single osteochondroma (5/408, 1.2%), and six were multiple osteochondromas (6/55, 10.9%). The male to female ratio was 10:1. These 11 cases were derived from femur (4 cases), tibia (3 cases), ilium (3 cases), shoulder bone (1 case) and pubis (1 case). There was one case that showed malignant transformation in both the femur and ilium. The mean ages for the malignant and non-malignant cases were 39.8 years and 20.4 years respectively. All the malignant cases showed large sized lesions with prominent calcification in the thick cartilage caps. The malignant component was low grade, peripheral chondrosarcoma (grade I-II). In some areas the tumor cells infiltrated the peripheral soft tissue and bone marrow. Of the seven cases with malignant transformation that had immunohistochemical staining, all were positive for vimentin and S-100 protein; p53 protein was positive in 2 of 7 cases., Conclusions: Malignant transformation of osteochondroma was usually encountered in multiple lesions. Most patients were more than 30 years old with a long clinical history and with a male predominance. These tumors showed thick cartilage caps with prominent calcification. The lobulated nature of the tumors was evident and they infiltrated the surrounding soft tissue. The sarcomatoid component was peripheral type, well differentiated chondrosarcoma. p53 mutation may explain part of the molecular mechanism in the malignant transformation.
- Published
- 2009
38. [Abnormal expression of c-myc, p53, p16 protein and GNAS1 gene mutation in fibrous dysplasia].
- Author
-
Tang J, Zhao HY, Zheng L, Zhang HZ, and Jiang ZM
- Subjects
- Adolescent, Adult, Child, Chromogranins, Female, Humans, Male, Middle Aged, Osteosarcoma genetics, Osteosarcoma metabolism, Osteosarcoma pathology, Young Adult, Cyclin-Dependent Kinase Inhibitor p16 metabolism, Fibrous Dysplasia of Bone genetics, Fibrous Dysplasia of Bone metabolism, Fibrous Dysplasia of Bone pathology, GTP-Binding Protein alpha Subunits, Gs genetics, Mutation, Proto-Oncogene Proteins c-myc metabolism, Tumor Suppressor Protein p53 metabolism
- Abstract
Objective: To study the significance of c-myc, p53 and p16 protein expression in fibrous dysplasia, to detect the GNAS1 gene mutation in fibrous dysplasia, and to explore the property of fibrous dysplasia., Methods: The expression of c-myc, p53 and p16 protein was evaluated by immunohistochemistry SP method in 35 cases of fibrous dysplasia including 1 FD with malignancy, 1 Mazabraud syndrome and 20 control cases (10 cases of bony callus, 10 cases of osteosarcoma). Genomic DNA extraction, PCR amplification and gene sequencing were used to detect GNAS1 gene mutation in 35 cases of fibrous dysplasia., Results: C-myc protein immunoreactivity was detected in 91 percentage of FD (P = 0.001). Compared with the negative control group, the difference was significant. P16 positive was detected in 34 FD cases (P = 0.001). The difference was significant as compared with the positive control group. Positive p53 protein expression was detected in the only 1 case of fibrous dysplasia with malignant transformation. PCR amplification was successful in 12 of 35 FD cases. Two of the 12 FD cases were detected to have GNAS1 gene mutation, in which 1 case was FD of Mazabraud syndrome, 1 case was a monostotic lesion., Conclusions: C-myc could be another protooncogene in addition to c-fos in the fibrous dysplasia disease. P53 protein overexpression could be useful in the diagnosis of FD malignancy and in the prediction of the prognosis of FD. The abnormal expression of the gene p16 might play an important role in the formation of FD. The GNAS1 mutation exist in FD. All of the results indicate that FD could be a neoplasia disease, caused by multiple factors leading to a dysfunction of bone development.
- Published
- 2009
39. [Analysis of polymorphic RAPD fragments in P. Taiwanensis Hayata using an integrated microflluidic chip-based system].
- Author
-
Tang JJ, Wu JL, Liu JH, Fan YR, and Zhu MY
- Abstract
Randomly amplified polymorphic DNA (RAPD) markers quickly provide linkage information, especially in conifers where haploid megagametophytes can be used for genotyping. Traditionally use of slab gel electrophresis results in qualitative data that can be manually manipulated to gain semiquantitative information about the polymorphic RAPD fragments. We have proposed the use of an integrated microfluidic chip-based system as a new tool in the analysis of polymorphic RAPD fragments. The chip-based method was found to be very sensitive,requiring much less sample and only quarter the time compared to the agarose gel method. The automated data analysis sizes and quantitates the DNA fragments, thus yielding a more thorough,reproducible, sensitive, and rapid analysis.
- Published
- 2003
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