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Copyright of Journal of Shenyang Pharmaceutical University is the property of Shenyang Pharmaceutical University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To investigate a method of rapidly screen monoclonal antibodies with high affinity to trophoblastic glycoprotein (TPBG) from the prepared monoclonal antibodies. Methods High-throughput surface plasmon resonance (SPR) technology was used to immobilize TPBG of different species on the GLC sensor chip by amino coupling. Gradient dilution of the antibody was used as the flow phase to screen monoclonal antibodies that can highly affinity TPBG, and the kinetic constants of the interaction were determined. Antibody 1 binds to human and cynomolgus monkey TPBG with affinity of 65.0 and 31. 6 nmol/L, respectively. Antibody 6 binds to human, mouse and cynomolgus monkey TPBG with affinity of 77.6, 92.1 and 87.7 nmol/L, respectively. Results This study successfully screened two monoclonal antibodies that can specifically bind to TPBG. Meanwhile, the specific binding of these two antibodies to TPBG protein was stable by binding kinetic. Conclusion The monoclonal antibodies with high affinity to TPBG protein are screened out by SPR, which provides a method for the development of some new anti-tumor drugs. [ABSTRACT FROM AUTHOR]

Copyright of Laser Technology is the property of Gai Kan Bian Wei Hui and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Exosomes are lipid bilayer membrane vesicles that are widely distributed in peripheral blood， saliva， urine， ascites and other fluids. A variety of tumor-related genes in exosomes are involved in the information exchange between cancer cells and normal cells， as well as the process of tumor cell proliferation and metastasis， play an important role in tumor development， and are potential biomarkers for tumor liquid biopsy. In recent years， surface plasmon resonance （SPR） is considered to have great application potential in the characterization of exosomes due to its high sensitivity， small sample size required for testing， short detection time and low background interference， etc. In this article， the basic principle of SPR and the application prospect of SPR-based biosensing platform in exosomes characterization were mainly illustrated.

Purpose: To investigate the binding interaction of astaxanthin with the transmembrane transporter protein cluster determinant 36 (CD36). Methods: Molecular docking was used to simulate the binding interaction between astaxanthin and CD36, the interaction parameters were obtained using an algorithm, the number of binding sites was determined, and the best matching model was established. Moreover, surface plasmon resonance (SPR) was used to measure the binding and dissociation constants of intermolecular interactions. Results: Astaxanthin entered the hydrophobic cavity inside CD36, and the docking binding energy was −11.70 kcal/mol, suggesting a dose-dependent effect. Besides, the equilibrium dissociation constant KD was 1.00 × 10-6 mol/L, indicating that astaxanthin bound to CD36 with high affinity, and the kinetic curves showed that the binding mode of astaxanthin and CD36 was slow binding and slow dissociation. Astaxanthin bound to more amino acids of CD36 through hydrophobic interaction. Unlike lutein and β-carotene, which formed weak van der Waals force with the polar amino acid Asn53 of CD36, astaxanthin formed hydrogen bonding with Asn53, which may be partly responsible for the high-affinity binding between astaxanthin and CD36. Furthermore, it was inferred that the ketone group on the terminal ring of astaxanthin or α-hydroxy ketone formed by hydroxyl and ketone groups played a certain role in the formation of hydrogen bonds. Conclusion: Astaxanthin interacts spontaneously with CD36 with high affinity, and the binding mode is slow binding and slow dissociation, contributing to efficient transmembrane transport.

The resource utilization of CO2 is of great significance to the realization of carbon cycle. Photocatalytic CO2 conversion is considered to be the greenest and cleanest method among various chemical CO2 utilization methods, but it has not been applied on a large scale due to its low reaction conversion efficiency. The plasmon photocatalysts can take advantage of the surface plasmon resonance phenomenon to effectively deal with the problems such as the difficulty of C=O bond activation, the insufficient utilization of light energy and the short life of photo-generated carrier, so as to improve the CO2 conversion efficiency and product selectivity. In order to promote the commercialization of photocatalytic CO2 conversion technology, the performance of plasmon photocatalytic CO2 conversion catalysts still needs to be further improved. Herein, by introducing the mechanism and discussing the recent research progress of plasmon photocatalytic CO2 conversion catalysts, the design ideas and regulation methods were summarized, the importance of improving reaction conversion efficiency and reducing catalyst cost was emphasized, and the future research and development direction was pointed out. [ABSTRACT FROM AUTHOR]

Pesticides play an important role in improving the yield and quality of agricultural products, but the irregular use of pesticides lead to excessive pesticide residues, which poses potential risks to people, livestock and the environment. For rapid monitoring of pesticide residues, rapid detection methods such as enzyme inhibition, enzyme-linked immunosorbent assay, colloidal gold immune chromatography, and biosensor have been widely used. Among them, optical sensors have attracted much attention for their high sensitivity and selectivity in the precise recognition and detection of pesticide molecules. This paper mainly introduces the precise recognition of pesticides by antibodies, aptamers, molecularly imprinted polymers, and metal-organic frameworks. The application of optical sensor based pesticide molecule identification technology in pesticide residue detection were reviewed from five aspects: colorimetry, fluorescence, chemiluminescence, surface-enhanced Raman spectroscopy, and surface plasmon resonance. The advantages, disadvantages, and development trend of the optical sensor based precise recognition technology for pesticide molecules was also summarized and prospected. This article provides a reference for the development of rapid detection technology for precise identification of pesticide residues. [ABSTRACT FROM AUTHOR]

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Exosomes are lipid bilayer membrane vesicles that are widely distributed in peripheral blood, saliva, urine, ascites and other fluids. A variety of tumor-related genes in exosomes are involved in the information exchange between cancer cells and normal cells, as well as the process of tumor cell proliferation and metastasis, play an important role in tumor development, and are potential biomarkers for tumor liquid biopsy. In recent years, surface plasmon resonance (SPR) is considered to have great application potential in the characterization of exosomes due to its high sensitivity, small sample size required for testing, short detection time and low background interference, etc. In this article, the basic principle of SPR and the application prospect of SPR-based biosensing platform in exosomes characterization were mainly illustrated. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Chongqing University of Technology (Natural Science) is the property of Chongqing University of Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Combined with template molecular pre-positioning, surface imprinting method, and post-modification strategy, a molecularly imprinted surface plasmon resonance (SPR) sensor was constructed on a gold substrate and used for detection of β-lactoglobulin (BLG) in camel milk powder. Firstly, the template molecule BLG was fixed by mercapto-terminated polyacrylic acid (PAA-SH) on the gold substrate which was modified by polydopamine in advance. Then imprinting was performed using dopamine as functional monomer and crosslinking agent. Finally, partially hydrolyzed poly (2-methyl-2- oxazoline) (PMOXA-EI) with anti-protein adsorption function was grafted on the non-imprinted part, and the BLG molecularly imprinted SPR sensor was prepared after eluting the template. The changes of sensor ’s surface hydrophilicity/hydrophobicity were studied by water contact angle (WCA) and the characterization of created sensor was performed by Fourier transform infrared spectrometer (FT-IR), atomic force microscopy (AFM), and variable angle [ABSTRACT FROM AUTHOR]

Bovine serum albumin (BSA) molecularly imprinted polymer (MIP) surface plasmon resonance (SPR) biosensors were prepared using BSA as the model, dopamine as the functional monomer and cross-linker and sodium periodate as the oxidant. BSA molecules were fixed in advance through hydrogen bond via introducing poly(acrylic acid) (PAA) to increase recognition sites, and partially hydrolyzed poly(2-methyl-2-oxazoline)(PMOXA-EI) was also introduced into the noncavity regions of polydopamine to resist nonspecific adsorption of proteins. The prepared BSA-MIP SPR sensor was characterized by X-ray photoelectron spectroscopy, atomic force microscopy, variable angle spectroscopic ellipsometry, and static water contact angle. SPR adsorption studies were carried out in aqueous BSA solutions with the mass concentration of 0.1 — 10 μg/mL. The limit of detection and the limit of quantification values were obtained as 53 ng/mL and 161 ng/mL, respectively. Selectivity studies were performed against β-lactoglobulin, ovalbumin, lysozyme, and cytochrome C, and the corresponding selectivity coefficients were determined to be 4.43, 3.45, 3.17 and 3.64, respectively. Finally, BSA-MIP biosensor was used to detect BSA in the mixture solution of the above five proteins. The BSA recovery rate was 97.5%—102.5%. [ABSTRACT FROM AUTHOR]

Near-infrared (NIR) light-induced photothermal therapy (PTT) has become a new method for precise tumor treatment due to its non-invasive, low toxicity, and targeted treatment. Gold nanoparticles have become an ideal photothermal agents due to its unique surface plasmon resonance (SPR), efficient photothermal conversion efficiency, low biological toxicity and good photostability. High quality imaging technology is a reliable and powerful tool for achieving effective photothermal therapy, especially multimodal imaging technology, which has excellent performance compared to a single imaging method, providing the possibility for more comprehensive and accurate tumor imaging, thereby significantly improving the potential of non-invasive medical treatment. Owing to their rich 4f electronic structure, NIR light excited rare-earth doped upconversion nanoparticles (UCNPs) exhibit multifunctional properties such as magnetism, fluorescence, X-ray attenuation, and radiation, making them an important application prospect in multimodal imaging as contrast agents. Therefore, constructing NIR-induced Au NPs/UCNPs composite nanosystems for multimodal imaging-guided photothermal therapy is expected to become a new strategy for cancer diagnosis and treatment. In this paper, we briefly introduced the optical properties of Au and UCNPs. In addition, this paper reviewed the latest research progress of PTT research on NIR induced UCNPs Au (nanoshell, nanorod, nanoclusters) composite nano system under the guidance of visual fluorescence imaging. The future prospects for achieving integrated diagnosis and treatment are presented, providing new ideas for its further research, development, and clinical application. [ABSTRACT FROM AUTHOR]

TiO2 nanotube arrays (TNTAs) were prepared by anodic oxidation with a tube diameter of 60 nm and a tube length of 5.4/im. Further Au- and Ag-modified TNT As/Au and TNT As/Ag nanotube arrays were prepared by electrodeposition. The effects of loading with different Au and Ag contents on the photoanodic photo-electrocatalytic conversion of glycerol-coupled hydrogen production coupling were evaluated. The results showed that TNT As loaded with Au and Ag could photo catalytically convert glycerol into high value-added products such as glyceraldehyde (GLI)) and dihydroxy ace tone (T)H A) and produce hyd rogen synergistically. TNT As/Au (0.08 m mol/L) and TNT As/Ag (0.04 mM) showed the highest catalytic performance with hydrogen production rates of 213.8/imol/cm²/h and 198.1/imol/cm1/h, glycerol conversion of 4.6 % and 4.4%, and T)H A selectivity of 15.4% and 15.3%, respectively. The best performance of the samples was attributed to the plasmon resonance effect of the metal nanoparticles, i.e., the metal acted as an electron trap to effectively gather the photoexcited electrons, and the electrons in the TiO2 conduction band were transferred to the metal to prevent the compounding of photogenerated electron-hole pairs, which effectively improved the catalytic performance of the TiO2 nanotube arrays. [ABSTRACT FROM AUTHOR]

Copyright of Acta Materiae Compositae Sinica is the property of Acta Materiea Compositae Sinica Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The latest research progress of hematite photoanode materials in the photoelectrochemical (PEC) water splitting is reviewed.The general and emerging strategies for improving the PEC properties of hematite photoanodes are summarized.General modification strategies include morphology control, impurity doping, hetero-structure construction, surface modification, etc.Emerging strategies include ultra-thin film resonant light trapping and surface plasmon resonance excitation.The effects of surface modifications on improving the water oxidation performance of PEC are highlighted, such as depositing passivation overlayers and loading co-catalysts of oxygen evolution reaction.The current challenges of hematite photoanode materials and their prospects in PEC water splitting are forecasted. [ABSTRACT FROM AUTHOR]

Copper based sulfide photocatalysts have attracted extensive attention of researchers due to their narrow band gap, local surface plasmon resonance effect, good absorption ability to visible light, rich in reserves and non-toxic properties. However, the high recombination rate of photogenerated electrons and holes, and the low utilization efficiency of visible light hinder their application in the field of photocatalysis. Therefore, researchers have tried different modification strategies to improve their photocatalytic performance. This paper focuses on the modification strategies of copper based sulfides, mainly discusses the modification of the photocatalytic performance of copper based sulfides by morphology regulation, crystal phase regulation, and semiconductor heterojunction, etc., and analyzes the effect of different modification methods on the improvement of the photocatalytic performance. The application of copper based sulfides in the photocatalytic degradation of organic pollutants, photocatalytic splitting water for hydrogen production, photocatalytic reduction of CO2, etc. are discussed, and the development direction of copper based sulfide modification is prospected. [ABSTRACT FROM AUTHOR]

Copyright of Journal of East China University of Science & Technology is the property of Journal of East China University of Science & Technology Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Mine Automation is the property of Industry & Mine Automation Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Liquid Crystal & Displays is the property of Chinese Journal of Liquid Crystal & Displays and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To establish a method for detecting human immunoglobulin Fc function based on surface plasmon resonance technology. Methods Based on the characteristic that FcγRI can be binded to the Fc segment of IgG, the affinity constant of the sample was detected by surface plasmon resonance, and its Fc function was the KD ratio of the sample to the standard. The method was validated for specificity/specificity, precision and robustness. The method and the pharmacopoeia method were used to detect the Fc function of 30 human immunoglobulins, and the correlation and consistency of the detection results were analyzed. Results The method validation results showed that this method has strong specificity/specificity (t values were 0.15, 0.22, both P>0.05), good precision (CV value 5.37%~10.69%) and good robustness (CV value 10.06%). The detection results of this method and the pharmacopoeia method have high correlation (r=0.96, P

Nearly all the life activities are regulated by the interactions of the protein with other biomolecules, including protein-protein interactions (PPIs) and protein-ligand interactions (PLIs), therefore understanding interaction and structural characterization of formed complexes represent a key task for modern bioscience. Conventional biophysical techniques like circular dichroism spectroscopy (CD), fluorescence resonance energy transfer (FRET), surface plasmon resonance (SPR), and isothermal titration calorimetry (ITC), are limited in studying protein complexes structures and interactions, considering sample purity is a major issue for obtaining reliable data with conventional methods mentioned above. Additional technologies are required to complement structural biology and interactomics research, while mass spectrometry (MS) can deal with impurities or even mixtures in principle, therefore native mass spectrometry (nMS) is demonstrated as a powerful tool to bridge the gap between structural biology and interactomics research. As the most widespread soft ionization technique, electrospray ionization is utilized in native mass spectrometry, which employs volatile aqueous solution, near physiological pH and gentle desolvation conditions to make the transfer of weakly bound noncovalent complexes into the gas phase while preserving their integrity and native structure possible. In this way, stoichiometry, binding partners, thermodynamics, structural dynamics, and topology information on transferred noncovalent complexes can be acquired. A recent breakthrough in native MS sample preparation demonstrated direct analysis of overexpressed proteins from crude cell lysates, which significantly simplified sample purification procedures. Innovations in mass spectrometry instruments, such as extended m/z range quadrupole stages or modification of Orbitrap instruments allow access to high mass with little compromise in resolving power. This has promoted the native analysis of protein complexes and diverse protein interaction networks to elucidate their architecture, as well as enabled the identification of small differences in proteoforms. While coupling with either ion mobility spectrometry (IMS) or capillary electrophoresis (CE), the analytical ability of native MS can be greatly extended. In this review, the technologies in native mass spectrometry and several cases in recent reports from five aspects were introduced, such as sample preparation, ion source, instrumentation, ion mobility mass spectrometry and coupling with capillary electrophoresis, challenges for the future development of native mass spectrometry. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Harbin University of Science & Technology is the property of Journal of Harbin University of Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Liquid Crystal & Displays is the property of Chinese Journal of Liquid Crystal & Displays and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Liquid Crystal & Displays is the property of Chinese Journal of Liquid Crystal & Displays and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In view of the common problems of fiber surface plasmon resonance sensor with weak strength, low sensitivity and low plasma excitation efficiency, this paper proposes to use the long-period fiber grating as an auxiliary platform to study the ways of enhancing the sensitivity of surface plasmon resonance sensors. A layer of gold film is thermally evaporated on the sensing area of the fiber grating surface, and the external sensing performance is enhanced by tuning the polarization mode of the input fiber grating. We choose the sodium chloride solution as the external refractive index sensing material, and conduct a simulation through software. The results show that before and after the gold coating, the transmission power of the transverse magnetic wave and the transverse electric wave are attenuated by 4.3 and 0.3 dB in the air, and 5.3 and 2.2 dB in the solution. Within the range of refractive index change of the solution from 1.335~1.372, the refractive index sensitivity of the two polarized light sources increased by 18.7 and 12.9 nm/RIU, respectively.

In view of the common problems of fiber surface plasmon resonance sensor with weak strength, low sensitivity and low plasma excitation efficiency, this paper proposes to use the long-period fiber grating as an auxiliary platform to study the ways of enhancing the sensitivity of surface plasmon resonance sensors. A layer of gold film is thermally evaporated on the sensing area of the fiber grating surface, and the external sensing performance is enhanced by tuning the polarization mode of the input fiber grating. We choose the sodium chloride solution as the external refractive index sensing material, and conduct a simulation through software. The results show that before and after the gold coating, the transmission power of the transverse magnetic wave and the transverse electric wave are attenuated by 4.3 and 0.3 dB in the air, and 5.3 and 2.2 dB in the solution. Within the range of refractive index change of the solution from 1.335~1.372, the refractive index sensitivity of the two polarized light sources increased by 18.7 and 12.9 nm/RIU, respectively.

Copyright of Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao is the property of Chinese Journal of Preventive Veterinary Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Chinese Institute of Food Science & Technology is the property of Journal of Chinese Institute of Food Science & Technology Periodical Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Besides the surface plasmon resonance characteristic of noble metal nanoparticles, the two-dimensional(2 D)noble metal nanoparticle ordered arrays can generate new or enhanced optical properties with good stability and reproducibility due to their highly ordered array structures on a large scale, which has drawn wide academic attention. This study introduces the main preparation methods of 2 D noble metal ordered nanoparticle array, including template method and self-assembly method. Compared with traditional photolithography, the template method and self-assembly method can not only obtain high-quality 2 D noble nanoparticle arrays, but also have lower economic cost. Additionally, the applications of the 2 D noble metal nanoparticle arrays in the fields of optical sensors, catalysis and others are summarized. However, the morphology of the 2 D noble metal nanoparticle arrays is comparatively simple, and more studies are expected to explore their properties.

Copyright of Journal of East China University of Science & Technology is the property of Journal of East China University of Science & Technology Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To find small molecules binding specifically to signal transducer and activator of transcription3 (STAT3) based on surface plasmon resonance (SPR) technology and confirm their inhibitory activities to STAT3. Methods The biomolecular interaction analysis T200 system based on SPR technology was used to couple the purified protein STAT3 to CM5 chip under the optimal pH conditions. The compounds with high binding response value were screened out from 50 candidate compounds derived from traditional Chinese medicines and the binding specificity was then confirmed. Biological experiments were performed to confirm the inhibitory effects of the screened compounds on STAT3. The binding pattern of STAT3 and the compound was fitted by molecular docking technique. Results More than 10 candidate molecules exhibited binding activities to STAT3 and kinetics assays revealed that only one candidate molecule, apigenin, showed specific binding. Western-blot analysis exhibited that apigenin inhibited the phosphorylation of STAT3 dose-dependently. Luciferase reporter gene assays demonstrated that apigenin also inhibited IL-6-induced STAT3 transcriptional activity in a dose-dependent manner. Molecular docking results showed that apigenin binds to the SH2 domain of STAT3, and interacts with key residues Glu638, Gln644, Gly656 and Lys658 by hydrogen bonds and with Tyr657 through π-π interactions. Conclusion Apigenin was a direct inhibitor of STAT3. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Chinese Institute of Food Science & Technology is the property of Journal of Chinese Institute of Food Science & Technology Periodical Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Fuzhou University is the property of Journal of Fuzhou University, Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The mixed polymer brushes which combine poly(2-methyl-2-oxazoline)(PMOXA) with anti-protein adsorption properties and poly(acrylic acid)(PAA) with stimulus-responsive properties were prepared on the material surfaces (including glass, silicon, and gold) by using poly(dopamine)(PDA) as an anchor. The surface chemical composition and thickness of the mixed polymer brushes were characterized by X-ray photoelectron spectroscopy (XPS) and variable angle spectroscopic ellipsometry (VASE). The hydrophilicity of the polymer brush surface was studied by water contact angle (WCA). Choosing pH = 9 and I = 0.01 mol/L (I is ionic strength) as the fibrinogen adsorption conditions, and pH = 9 and I = 0.15 mol/L as the fibrinogen desorption conditions. The adsorption and desorption behavior of fibrinogen on mixed polymer brushes was studied qualitatively and quantitatively by fluorescence microscope and surface plasmon resonance (SPR). The results showed that when the environmental pH = 9 and I changes from 0.01 mol/L to 0.15 mol/L, the surface of the mixed polymer brushes would change from a relatively hydrophobic state to a hydrophilic state. Increasing the degree of polymerization of PMOXA would reduce the amount of protein adsorption of the mixed polymer brushes, and would also significantly improve the protein desorption rate of the mixed polymer brushes. The mixed polymer brush prepared by sequential grafting PMOXA with a degree of polymerization of 60 and PAA with a degree of polymerization of 90 in a mass ratio of 3∶2 achieved the desorption rate of 83.5% for fibrinogen. [ABSTRACT FROM AUTHOR]

A rapid method for the screening of hazardous chemicals in textiles was developed using antireflection-laser desorption ionization mass spectrometry (AR-LDI-MS). An antireflection material was used to replace the small-molecule matrix used in traditional matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). This material has quasi-periodical array structure on its surface, which is fabricated using one-step direct writing with ultrafast laser on a stainless steel plate. Light can be trapped after multiple reflections inside the structure, accompanied by the excitation of surface plasmon resonance, which converts light energy to heat. With over 90% UV laser energy converted to heat, this material allows thermal desorption and ionization of target analyte on the surface. Since no matrix is used, no interference at low m/z area is found in AR-LDI-MS analysis, which allows the detection of analytes of low molecular weights. The antireflection material is also durable and reusable after careful cleaning. In the screening process, the analytes were extracted and transferred to the antireflection surface, followed by LDI-MS analysis. The pulsed UV laser had a pulse frequency of 1 kHz and a wavelength of 355 nm, which provided the energy for sample molecules’ desorption and ionization. A high voltage of 2 500 V was applied between the antireflection material and the mass spectrometer capillary to guide the ions into the mass spectrometer. The whole process could be completed in less than 1 min, avoiding time-consuming sample pretreatment and excessive solvent consumption compared with traditional chromatography or chromatography-mass spectrometry methods. Six hazardous chemicals in textiles were analyzed including phthalate esters, caprolactam, diethanolamine and triethanolamine. The limits of detection (LODs) of phthalate esters (1 mg/kg) and caprolactam (1 mg/kg) were comparable with traditional methods, but the LODs of diethanolamine (60 mg/kg) and triethanolamine (50 mg/kg) were relatively high. Quantitative analysis of phthalate esters was also performed using internal standards. Six textile samples purchased from local supermarket and internet were used for the screening of hazardous chemicals mentioned before by the method, and only caprolactam was detected in one sample. AR-LDI-MS is suitable for the rapid and high-throughput screening of textiles, and it can be also potentially used for the screening of other consumer goods, like food, cosmetics and detergents. Detection of chemical residues on the human skin or object surface may also be a possible application. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Donghua University (Natural Science Edition) is the property of Journal of Donghua University (Natural Science) Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Elucidating the quality markers(Q-markers) of traditional Chinese medicines is essential for understanding the mechanisms of action and promoting the rational use of traditional Chinese medicines as well as for developing traditional Chinese medicine-derived drugs. Studies have shown that surface plasmon resonance(SPR) is promising in this field. This study proposed a method based on pull-down with SPR chips to predict the Q-markers of Angong Niuhuang pills(AGNHP). Firstly, 71 main chemical components of AGNHP were analyzed by UPLC-Q-TOF-MS, and then network pharmacology was employed to predict the potential targets of AGNHP against stroke. Secondly, the STAT3 protein chip was constructed, and the extract of AGNHP was recovered by pull-down of the SPR system for STAT3 ligand. The potential active ingredients were collected, enriched, and identified as coptisine, palmatine, epiberberine, berberine, worenine, demethyleneberberine, jatrorrhizine, tetrahydrocoptisine, baicalein, and baicalin methyl ester. Next, the affinity constants of the 10 active ingredients were determined as 44.7, 44, 58.1, 51.3, 39.7, 32.1, 49.2, 69.1, 19.7, and 24.9 μmol·L~(-1), respectively. The molecular docking results showed that the 10 compounds could compete for binding with STAT3. This is the first report that SPR combined with UPLC-Q-TOF-MS is reliable and feasible for determining the active ingredients of AGNHP at the molecular level from complex systems. STAT3 could be used as a potential target for the biological quality evaluation of AGNHP.

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In order to obtain the actual position and normal vector information in the process of automatic drilling and riveting for aircraft components assembly，an on-line measurement method based on multi-sensor fusion included with vision and laser distance sensors is proposed. The method can obtain the correction of hole position deviation in real time and guarantee the quality of riveting and drilling. Firstly，by establishing the mapping relationship between the sensors and the reference coordinate system of the machine，the multisensor fusion model for on-line measurement of drilling and riveting holes is obtained. Besides，the on-line measurement principle of the hole position and orientation vector is given. Then，in order to simplify the calibration process and improve the accuracy of the calibration，a calibration target suitable for both vision and laser distance sensors is designed. Moreover，the calibration method about the position and orientation vector measurement is given. Finally，through the test，the position error of the hole position on-line measurement is no more than 0.2 mm，and the orientation error is within 0.3°，which can meet the accuracy requirements of hole measurement during aircraft drilling and riveting. [ABSTRACT FROM AUTHOR]

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