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Objective To investigate the effect of parent-child alienation on depression in surface ship officers and soldiers based on the theory of "diathesis-stress", and the mediating role of resilience between parent-child alienation and depression in them. Methods A cross-sectional study was conducted on 599 officers and soldiers from a surface ship unit.The participants were surveyed with inventory of alienation toward parents, connor-davidson resilience scale and patient health questionnaire-9 to obtain and analyze their demographic-military characteristics of their depression scores.The participants with depression scores ≥5 were recruited as the subjects, and Spearman correlation analysis was used to explore the correlation among parent-child alienation, resilience and depression.On the basis of hierarchical regression analysis, AMOS software was used to establish a structural equation modelling of intermediary effects. Results The depression score was 1(0, 4) in the participants, and the depression scores of those with service length ≥11 years were comparatively higher than those with shorter length.Our results indicated that parent-child alienation was positive correlated with depression (r=0.451, P < 0.001), while resilience was negatively correlated with depression and parent-child alienation (r=-0.412, -0.407, P < 0.001).Regression analysis revealed that parent-child alienation had a direct positive predictive value for depression (β=0.574, P < 0.001), and resilience showed a negative predictive value for depression (β=-0.211, P < 0.01).Model analysis displayed that resilience had a significant mediating role in the effect of parent-child alienation on depression among these surface ship officers and soldiers, with an effect value of 0.088, and accounting for 15.86% of the total effect. Conclusion Parent-child alienation has a significant influence on depression among surface ship officers and soldiers, with resilience playing a partial mediating role.

In order to improve the added value of foxtail millet and the processing quality of foxtail millet noodles, the effect of different periods (0.5, 1, 2, 4, 6, and 8 h) of ball milling on the nutritional components, hydration properties, gelatinization properties, rheological properties and crystalline structure of foxtail millet flour and the quality of the resulting noodles was investigated. The correlation between some physicochemical properties and noodle quality was analyzed. The results showed that the crystalline structure of foxtail millet flour was damaged obviously, and the damaged starch content increased significantly with an increase in milling time. It was also found that the amylose content decreased continuously (except for 8 h), and the L* value rose from 77.27 to 81.37. The peak viscosity, trough viscosity, and final viscosity of foxtail millet flour increased from 1 478, 665 and 1 384 Pa·s to 3 271 (ball milling 1 h), 982 (ball milling 2 h) and 2 199 (ball milling 2 h) Pa·s (P < 0.05), respectively, and the hydration properties (water-holding capacity and swelling capacity) improved (P < 0.05). Ball milling for 4 h made the gel network structure of dough more compact and resulted in noodles with the best texture and cooking quality. Correlation analysis showed that noodle texture properties (elasticity, stickiness and chewiness) and sensory quality (color, appearance and stickiness) were significantly negatively correlated with amylose content (P < 0.01), but significantly positively correlated with damaged starch and amylopectin contents (P < 0.01). The cooking quality (breakage rate) of noodles was positively correlated with amylose content (P < 0.01), but significantly negatively correlated with damaged starch and amylopectin contents (P < 0.01) and gelatinization viscosity was also strongly correlated with noodle quality. Taken together, the modification of foxtail millet flour by ball milling can be used to produce high-quality foxtail millet noodles.

Objective To investigate the effects of childhood trauma on early-adulthood depression and the mediating roles of neuroticism, negative self-description, fancy, life-events, social support and resilience in the process based on a vulnerability stress model through a 4-year longitudinal study. Methods In October 2016 (T1), a total of 510 freshmen from a university were recruited and surveyed with Beck depression inventory (BDI-Ⅱ), Childhood Trauma Questionnaire-28 item Short Form (CTQ-SF), Eysenck personality questionnaire (EPQ), Self-description Task (SDT), Coping Style Questionnaire (CSQ), Adolescent Self-rating Life Events Checklist (ASLEC), Social Support Rating Scale (SSRS), and Connor-Davidson Resilience Scale (CD-RISC). Then in October 2019 (T4), 433 of them were assessed with BDI -Ⅱ, ASLEC, SSRS and CD-RISC. Structural equation model was used to analyze the potential mediating mechanism of childhood trauma on depression. Results ① Childhood trauma was positively correlated with depression at T1 and T4 (r=0.27, 0.15, P < 0.01). ②Stepwise regression analysis showed that childhood trauma, neuroticism, negative self-description, fancy, life-events, and resilience could predict early-adulthood depression, and explained 53% of depression at T1. ③Structural equation modeling indicated that childhood trauma positively predicted early-adulthood depression, and neuroticism, negative self-description, fancy, life-events and resilience partially exerted mediating roles (effect value=0.25, P < 0.01). ④Instant model further suggested that childhood trauma had a direct impact on T1 depression (effect value=0.09, P < 0.05), and exerted immediate mediating effects through resilience, life-events and social support (effect value=0.13, P < 0.01). What's more, childhood trauma also showed delayed mediating effects on T4 depression through above mentioned variables at T1 and T4 (effect value=0.14, P < 0.01). Conclusion Childhood trauma can positively predict early-adulthood depression, and neuroticism, negative self-description, fancy, life-events, and resilience show mediating effects between childhood trauma and depression. And resilience, life-events, and social support exert immediate and delayed mediating roles between them.

Objective To reveal the possible effects of childhood trauma on resilience in early adulthood and explore its mediating and moderating mechanisms. Methods A series of follow-up questionnaire surveys were conducted in 4 consecutive years from October 2016 (T1) to October 2019 (T4) among the undergraduates of a military university. The evaluation tools included Childhood Trauma Questionnaire, Connor-Davidson Resilience Scale, Self-Description Scale, Cognitive Flexibility Inventory, Eysenck Personality Questionnaire, General Self-Efficacy Scale, Social-Support Scale, Coping Style Questionnaire, Adolescent Self-Rating Life-events Checklist, and Beck Depression Inventory. The questionnaire scores were analyzed using correlation, regression and structural equation model. A total of 510 college students participated in the survey at T1, and 433 of them finished the whole 4-year investigation. Results ① Childhood trauma were negatively correlated with the resilience scores of the 4 successive years (r=-0.36~-0.12, P < 0.05). ② Regression analysis showed that childhood trauma, depression, extroversion, positive self-description, self-efficacy and cognitive flexibility played predictive roles in the resilience score (β=-0.22~0.34, P < 0.05). ③The total score of childhood trauma presented a direct negative predictive effect on resilience (β=-0.12, P < 0.001), and indirectly predicted resilience through mediators of extroversion, problem solving, positive self-description, self-efficacy and cognitive flexibility (indirect effect value -0.41, P < 0.001). ④The total score of childhood trauma exerted a stable negative effect on the score of resilience during T1, and a complete mediating effect during T2 and T4, and the delayed mediating effect during T1 and T4 stably affected the score of resilience in a negative model (effect value: -0.11~-0.05, P < 0.05). ⑤ Depression score had moderating effects in the comprehensive model as well as in the T3 instant model (Δχ2=36.45~23.58, ΔP < 0.01). Conclusion The total score of childhood trauma shows direct and negative predictive effect on the early-adulthood resilience, and has a continuously negative prediction through a mediation-moderate mechanism. The impacts of life events stress, as well as social support on resilience, have the characteristics of immediate and delayed time.

The pulse amplitude of fingertip volume could be improved by selecting the vascular dense area and applying appropriate pressure above it. In view of this phenomenon, this paper used Comsol Multiphysics 5.6 (Comsol, Sweden), the finite element analysis software of multi-physical field coupling simulation, to establish the vascular tissue model of a single small artery in fingertips for simulation. Three dimensional Navier-Stokes equations were solved by finite element method, the velocity field and pressure distribution of blood were calculated, and the deformation of blood vessels and surrounding tissues was analyzed. Based on Lambert Beer's Law, the influence of the longitudinal compression displacement of the lateral light surface region and the tissue model on the light intensity signal is investigated. The results show that the light intensity signal amplitude could be increased and its peak value could be reduced by selecting the area with dense blood vessels. Applying deep pressure to the tissue increased the amplitude and peak of the signal. It is expected that the simulation results combined with the previous experimental experience could provide a feasible scheme for improving the quality of finger volume pulse signal.

With the rapid development of transgenic technology, the safety of genetically modified products has received extensive attention. Certified reference materials for the detection of genetically modified organisms play important roles in ensuring comparability and traceability of the qualitative and quantitative detection of genetically modified products. However, the development of protein reference materials is relatively slow, and one of the difficulties is the preparation of protein candidates with high purity. The cry1Ah1 gene of Bacillus thuringiensis has been used for the development of transgenic insect-resistant crops because of its excellent insecticidal activity against lepidopteran pests such as Asian corn borer, and has obtained transgenic lines with good insect resistance traits. In order to develop Cry1Ah protein certified reference material, it is urgent to establish a preparation and purification system. In this study, a system for preparing Cry1Ah protein by Bt expression system was optimized, and a high-purity Cry1Ah protein (size exclusion chromatography purity: 99.6%) was obtained by ion-exchange chromatography and size exclusion chromatography stepwise purification. The results of biological activity assay showed that there was no significant difference in the insecticidal activity of purified Cry1Ah protein and protoxin against diamondback moths (Plutella xylostella). Finally, the amino acid sequence of the activated Cry1Ah protein was determined using Edman degradation and mass spectrometry. In summary, the obtained Cry1Ah pure protein can be used for the development of protein reference materials.