Search

Objective To investigate the role of BEND6 in regulating gene expression in mouse neural stem cells (NSCs) and to explore its biological function during neural development. Methods pCMV-mScarlet (control group) and pCMV-mScarlet-BEND6 (experimental group) plasmids were transiently transfected into mouse NSCs, respectively. After 24 hours, the expression of mScarlet and mScarlet-BEND6 proteins was confirmed by the presence of red fluorescence. Total RNA was then extracted from the two groups of cells for RNA-seq. Through reference genome alignment, quantitative analysis, differential gene expression analysis and enrichment analysis of the sequencing data, we were able to profile the transcriptome of NSCs upon BEND6 overexpression and to suggest a function for mouse neural development. Results Mouse NSCs were successfully isolated for primary culture. Whole transcriptome sequencing analysis revealed 165 differentially expressed genes after force-expressing BEND6, among which 102 genes were up-regulated and 63 genes were down-regulated. Enrichment analysis showed that these differentially expressed genes were highly related to the regulation of membrane potential change, synaptic transmission, stimulation response, growth and development and other biological processes. Conclusions BEND6 regulates the expression of genes related to neuronal functions in mouse NSCs, which suggests a potential mechanism in regulating neuron specific biological processes during development.

Copyright of Chinese Journal of Geological Hazard & Control is the property of China Institute of Geological Environmental Monitoring (CIGEM) Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Science & Technology of Food Industry is the property of Science & Technology of Food Industry Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Rehabilitation Medicine is the property of China Association of Rehabilitation Medicine, China-Japan Friendship Hospital and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Tropical Geography is the property of Tropical Geography Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of South China University of Technology (Natural Science Edition) is the property of South China University of Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Industrial Water Treatment is the property of CNOOC Tianjin Chemical Research & Design Institute and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Inorganic Analytical Chemistry / Zhongguo Wuji Fenxi Huaxue is the property of Beijing Research Institute of Mining & Metallurgy Technology Group and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

(Objective) Jujube is one of the cash crops in northern Shaanxi. Understanding the suitable areas and conditions for its growth is important to its sustainable production but is currently lacking. This paper is to fill this knowledge gap. (Method) The analysis is based on GIS; the suitability for jujube growth in different areas is analyzed using the geographical detector model. (Result) 1 The suitability for jujube growth decreases progressively from the east and south to the northwest of the region, with the optimal area and sub-optimal area concentrated in Fu county. Suitable areas with large jujube planting are mainly in the districts and counties along the western region of the Yellow River. 2 Climatic and topographic factors combine to greatly influence the suitability of jujube, and their effects are nonlinearly confounded. 3 Annual precipitation, elevation and annual accumulative temperature (≥10 °C) are the main factors controlling suitability for jujube growth, and the suitability index is negatively correlated with annual precipitation and annual accumulated temperature, and positively with elevation. 4 Annual precipitation, elevation and annual accumulated temperature explain 22%, 15% and 11% of the suitability index, respectively, and the comprehensive interpretation rate reaches 78%.(Conclusion)The suitability for jujube growth in northern Shaanxi province decreases from the southeast to the northwest; developing jujube industry or forest ecosystem in this region should consider the jointed effects of annual precipitation, elevation and annual accumulated temperature. [ABSTRACT FROM AUTHOR]

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Transactions of the Chinese Society of Agricultural Engineering is the property of Chinese Society of Agricultural Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Transactions of the Chinese Society of Agricultural Engineering is the property of Chinese Society of Agricultural Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)