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According to the characteristics of the inner wall of curved pipe with variable cross-section and the inner wall of straight pipe, the mathematical model was established for the demolding force of the inner wall of curved pipe by using a calculus method, and the demolding force of the core of connecting pipe was calculated. Based on the obtained results, the demolding mechanism of the inner wall of curved pipe was optimized and designed for the plastic part, and this mechanism was driven by a single oil cylinder to rotate and pull out the core of the two-sector gear bent pipes. On the basis of the demolding mechanism,set in this work, the mold structure of plastic part was composed of a mold with two cavities and a single opening and two-plate mold. In this mold, there are two groups of external thread demolding mechanisms with a Haver slider and a sector gear bent pin core-pulling mechanism driven by a linkage oil cylinder. The die structure is simple and practical, and the action of mechanism is reliable. This design can offer a good reference for design of other similar molds. [ABSTRACT FROM AUTHOR]

In recent years, Chinese hamster ovary (CHO) production vessel volume has reached more than 1 000 L in Chinese biopharms, and 10 000 L in foreign big biopharms, such as Lonza and Genetech. In general, there are some steps seed bioreactor for seed expansion, which decreases the efficiency of production process. In this work, a perfusion-based process was developed to drastically increase the split ratio during the scale-up of CHO cell cultures. Fed-batch cultures were inoculated with cells propagated in either batch or perfusion cultures that grown in disposable Cellbags using the WAVE Bioreactor system. The higher cell concentration of 2 x 10(7) cells/mL with 95% viability allowed to increase the split ratio to about 1:50-1:100 for inoculum propagated in perfusion culture. The method described here could reduce the number of required expansion steps and eliminate two or three bioreactors. Disposable perfusion bioreactor with only a few liters working volume have the potential to directly inoculate volumes of up to 1 000 liters. This would allow to shorten process time in these bioreactors, which often are the bottleneck in plant throughput.