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The satisfaction of residents plays a pivotal role in the sustainable renovation and development of old residential communities. This paper employs the neighborhood effect theory and the organic renewal theory to investigate the satisfaction of residents in the renovation of old residential communities. It employs the questionnaire survey method to collect data from residents of old residential communities in Baiyin City and analyzes the data using a structural equation model (SEM). The findings indicate that the content of the renovation, the behavior of the government, the participation of residents, and the emotion of the community have a significant positive impact on residents' satisfaction. Additionally, the behavior of the government has a significant positive impact on the content of the renovation, and the content of the renovation has a significant positive impact on the emotion of the community. The relationship between government behavior and residents' satisfaction is mediated by residents' participation and the renovation content. Similarly, the relationship between the renovation content and residents' satisfaction is mediated by community emotion and residents' participation. Consequently, in the context of future renovations of old residential communities in China, it is crucial for grassroots government organizations to optimize the content of the renovations in accordance with the needs of residents, and to facilitate multi-faceted participation by residents. This approach is essential for the creation of harmonious neighborhood relationships and the improvement of residents' satisfaction, which are both vital for the sustainable transformation of old residential communities in China. [ABSTRACT FROM AUTHOR]

In order to discover the precursory characteristics and the mechanism of coal bumps by faults with extra thick and hard roof, the No. 25110 working face of Yuejin Coal Mine in Yima was investigated to analyze the law of coal bumps in a certain period. The law of time and space of microseismic energy and frequency and microseismic activity were analyzed with the combination of the microseismic monitoring data based on the typical cases of coal bumps induced by faults in the condition of extra thick and hard roof. The distribution characteristics of stress fields near faults under mining influence was studied by numerical simulation. The causes and mechanism of coal bumps of the face were discussed from three aspects of geological structure, microseismic activity and stress fields. The results show the extra thick and hard roof is cut by F16 thrust faults and two normal faults, which can cause a large roof movement, and provide a condition for the dynamic load of coal bumps. The microseismic energy and frequency decrease, and the microseismic activities near faults reduce. This is the evidence of microseism phenomenon reduction, while the energy is accumulated for coal bumps. High strength abutment pressure is formed by extraction, faults and the goaf of adjacent face. High stress concentration is formed from the faults to No. 25110 lower roadway. Especially high horizontal stress is in the floor of lower roadway. The conditions of power source are provided for coal bumps. [ABSTRACT FROM AUTHOR]

This research aims to investigate the encapsulation and controlled release effect of the newly developed self-assembling peptide R-LIFE-1 on exosomes. The gelling ability and morphological structure of the chiral self-assembling peptide (CSAP) hydrogel were examined using advanced imaging techniques, including atomic force microscopy, transmission electron microscopy, and cryo-scanning electron microscopy. The biocompatibility of the CSAP hydrogel was assessed through optical microscopy and fluorescent staining. Exosomes were isolated via ultrafiltration, and their quality was evaluated using Western blot analysis, nanoparticle tracking analysis, and transmission electron microscopy. The controlled release effect of the CSAP hydrogel on exosomes was quantitatively analyzed using laser confocal microscopy and a BCA assay kit. The results revealed that the self-assembling peptide R-LIFE-1 exhibited spontaneous assembly in the presence of various ions, leading to the formation of nanofibers. These nanofibers were cross-linked, giving rise to a robust nanofiber network structure, which further underwent cross-linking to generate a laminated membrane structure. The nanofibers possessed a large surface area, allowing them to encapsulate a substantial number of water molecules, thereby forming a hydrogel material with high water content. This hydrogel served as a stable spatial scaffold and loading matrix for the three-dimensional culture of cells, as well as the encapsulation and controlled release of exosomes. Importantly, R-LIFE-1 demonstrated excellent biocompatibility, preserving the growth of cells and the biological activity of exosomes. It rapidly formed a three-dimensional network scaffold, enabling the stable loading of cells and exosomes, while exhibiting favorable biocompatibility and reduced cytotoxicity. In conclusion, the findings of this study support the notion that R-LIFE-1 holds significant promise as an ideal tissue engineering material for tissue repair applications.

To investigate the whole-reach nitrate (NO 3 - -N) uptake dynamics in a headwater agricultural stream, we performed five pulse tracer additions of a reactive solute (as KNO 3 ) and a conservative solute (as NaBr) in an agricultural drainage ditch in Hefei district, Chaohu Lake basin, from October 2016 to April 2017. The TASCC (tracer additions for spiraling curve characterization) approach and Michaelis-Menten (M-M) method were applied for the simulation of NO 3 - -N uptake dynamics. Results showed that the ambient areal rate of total NO 3 - -N uptake U amb varied from 11.40 to 69.13 μg ·(m 2 ·s) -1 with an average of 34.45 μg ·(m 2 ·s) -1 , and the ambient uptake velocity V f-amb averaged 0.24 mm ·s -1 and varied from 0.07 to 0.43 mm ·s -1 across three well-mixed sub-reaches in the study. The ambient uptake length S w-amb averaged 199.06 m with a range from 92.51 to 405.74 m, which was much smaller than the length of the drainage ditch (about 2.5 km), suggesting that the agricultural drainage ditch had a high potential for NO 3 - -N retention. Generally, the M-M model fit the NO 3 - -N uptake dynamics well, and the maximum uptake U max ranged from 158 to 1280 μg ·(m 2 ·s) -1 with a mean of 631.13 μg ·(m 2 ·s) -1 . The half saturation constant K m ranged from 0.16 to 5.52 mg ·L -1 with a mean of 1.46 mg ·L -1 . According to correlation analysis, S w-amb was negatively correlated with NO 3 - -N amb , and U amb was significantly positively correlated with NO 3 - -N amb , while other nutrient spiraling metrics were not correlated with the NO 3 - -N ambient concentration. Hydrological conditions had no distinct effect on the NO 3 - -N retention, but both the width variability Ф w and variability in cross-sectional area Ф A were significantly correlated with most of the nutrient spiraling metrics, indicating that geomorphic features in the drainage ditch evidently impacted NO 3 - -N uptake.

Water and benthic sediment samples were collected monthly from six sites over a 2-km reach in a first-order stream located in the northeastern rural-urban fringe of Hefei City, from May 2016 to January 2017. These sites were scattered in three stream reach types as natural pattern sections (Scenario 1), point source pollution sections (Scenario 2), and severe soil erosion sections (Scenario 3). The potential rates of nitrification and denitrification in the sediments were measured and variable characteristics were evaluated quarterly. Moreover, a difference analysis of each of the three scenarios and an influencing factor analysis for nitrification and denitrification potentials were subsequently conducted. Our results show that:① the mean value of the nitration ratios for total sediment samples is 0.381%, of which the maximum nitration ratio emerged in Scenario 2. As for Scenario 1, the nitration ratio in summer is the larger than in winter. The nitration ratios for Scenarios 2 and 3 rank from largest to smallest as spring > summer > autumn > winter. ② The mean value of the nitrification rate of the total sediment samples is 0.364 mg·(kg·d) -1 , of which the maximum nitrification rate is associated with in Scenario 2. The nitrification rate is highest in winter, while similar for all other seasons in Scenarios 2 and 3. There is not much difference all year round for Scenario 1. ③ Average values for the denitrification ratio and denitrification rate for all sediment samples are 37.25% and 57.68 mg·(kg·d) -1 , respectively. Both of these are higher for Scenario 2 than the other two scenarios for the same season. The ranking for denitrification for Scenarios 1 and 2 is summer > spring > autumn > winter, and spring > summer > autumn > winter in Scenario 3. ④ According to the difference analysis, significant differences not only exist between the nitrification rates between Scenarios 1 and 2 but also for the denitrification ratios and denitrification rates between Scenarios 1 and 3. In addition, there is an obvious difference in the nitration ratio between Scenarios 2 and 3. ⑤ Partial least-squares regressive analysis indicates that there is significant difference between the important influencing factors related to the nitration ratio and nitrification rate for each of the three scenarios.

To reveal the impact of land use change on the phosphorus uptake in benthic sediments of suburban streams, a headwater stream in the urban fringe of Hefei City was selected and a set of benthic sediments was collected monthly from the chosen stream reach from June to November 2016. An incubation method was applied to explore the biotic and abiotic uptake of phosphorus in benthic sediments under intense human disturbance scenario. Results showed that the uptake potentials in summer were higher than those in autumn, both for total (including biotic and abiotic) and abiotic uptake of phosphorus. Furthermore, both of these uptakes were distinctly higher for the third sampling site, which is adjacent to the sewage outlet, than those for the other sites. For all six sampling sites, the contribution rate of biotic uptake of phosphorus was significantly greater than that of abiotic uptake, both in summer and autumn. The monthly variations in potentials and contribution rates of phosphorus uptake indicated that intense human disturbance via land use change had a great impact on the biotic uptake of phosphorus in benthic sediments of the suburban stream.

From May 2015 to June 2016, seasonal sediment samples were collected from three headwater streams in the urban-rural fringe of Hefei, Chaohu Lake basin, China. The nitrogen pollution characteristics of sediments were preliminarily investigated for the three streams. Three metrics, that is, potential nitrification rate (PNR), areal nitrification rate (ANR) and rate of denitrification were quantitatively determined, and their spatial and temporal variations were discussed subsequently. Moreover, the relationship between PNR, ANR or denitrification rate and a list of environmental factors was discriminated by using partial least-squares regression analysis. Results showed that:① Guanzhenhe Distributary was the most polluted stream with a mean content of 4516.39 mg·kg -1 for TN, which was 2.56 and 1.36 times higher than those of Modian Stream and Taochong Stream, respectively. Similarly, the highest values for NH 4 + -N and NO 3 - -N emerged in Guanzhenhe Distributary while the lowest existed in Modian Stream. ② There were distinct seasonal variations in PNR and ANR, namely, the maximum values emerged in summer, minimum in winter, and almost equivalent in spring and autumn. And the arrangement in order for PNR and ANR was Taochong Stream > Modian Stream > Guanzhenhe Distributary. ③ The mean rate of denitrification in Guanzhenhe Distributary was 10.59 mg·(kg·h) -1 , which was 3.16 and 1.75 times higher than those in Modian Stream and Taochong Stream, respectively. In addition, both denitrification rate and denitrification activity in summer were higher than those in spring for the three streams. ④ According to the variable importance plot ( F VIP ), ANR, PNR and rate of denitrification were almost all significantly correlated with such physical and chemical factors as pH, OM, NH 4 + -N, NO 3 - -N, TN and TP in sediments for the three streams.

A typical water system of agricultural headwater stream in Chaohu Lake basin was selected as the study area, and 17, 16, 14 and 13 surface sediments were collected from the four styles of stream, respectively, including ponds, branches, main channel and mainstream deep pools, in October 2014 (in autumn) and April 2015 (in spring). The forms and space-time variations of phosphorus in the sediments were analyzed. Clustering and variance analysis were conducted on the phosphorus forms data from the four styles of stream by means of multivariate statistical analyses. We quantified the phosphorus release risk (PSI) and identified the main impact factors of PSI via calculating the phosphorus sorption index (PSI) and the correlation analysis. The results showed that: (1) The contents of TP in the surface sediments ranged from 137.517 to 1709.229 mg x kg(-1) with an average value of 532. 245 mg x kg(-1), and the order of the average contents of phosphorus forms was IP (350.347 mg x kg(-1)) > OP (167.333 mg x kg(-1)) > Fe/Al-P ( 78. 869 mg x kg(-1)) > Ca-P (56.343 mg x kg(-1)) > Ex-P (6.609 mg x kg(-1)); (2) The contents of phosphorus forms had the same trend in all the four stream styles, which was deep pool > main channel > branch > pond; (3) In autumn, the deep pool and main channel were clustered into one class, while the pond and branch were clustered into the other class. In spring, branch, main channel and deep pool were clustered into the same class; (4) Variance analysis showed that the differences among the four stream styles were larger in autumn than in spring; (5) The PSI of the surface sediments ranged between 24.49 and 69.94 (mg x L(-1) x (100 g x micromol)(-1). The PSI in spring was lower than that in spring, indicating that phosphorus release risk of surface sediment was higher in spring than in autumn. (6) PSI had a significant negative correlation with Ex-P, IP and pH.

A micellar electrokinetic chromatography-mass spectrometric method based on laurel acyl malic acid ester (LMAE) for the separation and determination of coptisine, berberine, jatrorrhizine, phellodendrine and ligustrazine in Niuhuang Shangqing Tablets was established. The baseline separation of the five compounds was attained within 18 min by an uncoated capillary (88 cm x 50 microm) on the operating voltage of 25 kV using 7.5 mmol/L LMAE-15 mmol/L ammonia-50 mmol/L ammonium acetate mixture (pH = 7.0) containing 12.5% (v/v) acetonitrile as the electrophoretic medium and 50% 2-propanol aqueous solution (containing 3 mmol/L acetic acid) as the sheath liquid. The peak area of each component to its concentration showed a good linear relationship. The relative standard deviations of migration times and peak areas of the five components were less than 5% and the recoveries were between 96.0% and 105%. The developed method is simple, rapid, accurate and is suitable for the routine analysis of the five alkaloid components in Niuhuang Shangqing Tablets.

Aim: To study the effects of inosine on myosin heavy chain (MHC) activity in rats soleus muscle after tail-suspension., Methods: Weightlessness was simulated by tail-suspension of rats. Using immunohistochemistry technique, changes of expression of MHC in intrafusal and extrafusal fibers in soleus muscle were detected., Results: Expression of fast MHC in intrafusal and extrafusal fibers in soleus muscle increased after 14 d of simulated weightlessness, whereas in tail-suspended rats given inosine, the expression of fast MHC was not detected., Conclusion: Inosine could counteract the changes in expression of MHC in intrafusal and extrafusal fibers of rats soleus under simulated weightless situation.

Objective: To study the effects of Ligustrazine (Lig) and Radix Astragali (Rad) on activities of myosin adenosine triphosphatase (mATPase) of soleus muscle and atrophy in tail-suspended rat., Method: Weightlessness was simulated by tail suspension in female rats. The activities of mATPase of intrafusal and extrafusal fibres in soleus muscle were detected by method of Ca2(+)-ATPase., Result: 1) Compared with tail-suspended (TS) group, the percentage of type II fibres of SOL in both Ligustrazine (Lig) group and Rad group decreased distinctly. Furthermore, the percentage of type I and type II fibres of SOL in Rad group showed no difference with control (CON) group. 2) The type I CSA of Lig group was markedly larger than that in TS group, and there was no difference as compared with that of CON group. CSA of type I, II and average CSA of Rad group were remarkably larger than those in TS group, and there were no difference as compared with those of CON group. 3) mATPase activities of intrafusal fibres in both Lig group and Rad group were approximate to that of CON group., Conclusion: Lig and Rad are both able to effectively prevent muscle atrophy caused by tail suspension, restrain the slow-twitch muscle transform to fast-twitch muscle and control the increase of mATPase activities caused by weightlessness.

Objective: To study the effects of high frequency vibration on expression of myosin heavy chain (MHC) in intrafusal and extrafusal fibers in soleus muscles of tail-suspended rats., Method: Weightlessness was simulated by tail suspension of female rats. Using immunohistochemistry technique, changes of expression of MHC in intrafusal and extrafusal fibers of soleus muscles were detected., Result: Expression of fast MHC in intrafusal and extrafusal fibers of soleus muscles increased during 7 d of simulated weightlessness, whereas during 7 d of tail suspension plus high frequency vibration, these changes were not detected., Conclusion: High frequency vibration can counteract the changes in expression of MHC in intrafusal and extrafusal fibers of rats soleus under muscles of rats in simulated weightlessness situation.

Objective: To study changes in structure and innervation of soleus muscle spindle in rats induced by simulated weightlessness., Method: Weightlessness was simulated by tail suspension in female rats. The technique of Fawosky's Staining was used to detect the morphology of nerve endings of soleus muscle spindle in 7 d, 14 d, 21 d tail-suspended and control rats., Result: The intrafusal muscle fibers were regular and nerve endings were intact and clear in the soleus spindles of control rats. No obvious change was observed in muscle spindles of 7 d tail-suspended rats. Intrafusal muscle fibers became irregular, with rough and vague nerve endings in 14 d tail-suspended rats. The distinct retrograde change of muscle spindles and nerve endings were observed in 21 d tail-suspended rats. Degeneration and rupture were found in nerve endings, which made it unclear and pale in staining., Conclusion: Simulated weightlessness could induce time-related changes in structure and innervation of soleus muscle spindle in rats.

Objective: To study changes in ultrastructure of soleus muscle spindle induced by simulated weightlessness in rats., Method: Weightlessness was simulated by tail suspension in female rats. The ultrastructure of isolated soleus muscle spindle were observed in 4 d, 7 d, 14 d tail-suspended and 14 d recovered rats., Result: Chaotic myofibril, hyperplasia of mitochondria, and enlargement of terminal cisterna were found in the ultrastructure of isolated soleus muscle spindle in 4 d tail-suspended rats. The ultrastructure of isolated soleus muscle spindle in 7 d tail-suspended rats showed more distinct changes, while that in 14 d tail-suspended rats showed obvious retrograde changes. The ultrastructure of isolated muscle spindle in 14 d recovered rats after 14 d tail-suspension were similar to that of control group., Conclusion: Simulated weightlessness could induce time-related and reversible changes in ultrastructure of soleus muscle spindle in rats.