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Objective Using transcriptome sequencing, we screened differentially expressed transcription factors (TFs) in keratinocyte (KC) with overexpression of the epidermal growth factor receptor (EGFR) induced by recombinant human parathyroid hormone (1-34) in order to investigate the mechanism of rhPTH(1-34) in the treatment of psoriasis. Methods Overexpression of EGFR in HaCaT cells was achieved by lentiviral transfection. Cells in rhPTH (1-34) group were incubated with 0.01 μmol/L of rhPTH (1-34), while the control group were treated with DMEM solution alone. After extraction of RNA, the quantity and quality of RNA were assessed using UV absorbance assay and denaturing agarose gel electrophoresis, respectively. The cDNA was synthesized and amplified by real-time quantitative PCR for accurate quantification of the effective concentration of the library. DESeq2 software was used to analyze the differentially expressed genes, and significance was defined as |log2foldchange|≥1 and P

Objective To assess the clinical efficacy and safety of sequential therapy for nodular cystic acne. Methods Forty three patients with nodular cystic acne were divided into sequential and control groups. In the sequential group, 22 patients were treated with ALA-PDT every two weeks for 3 times, and oral antibiotics in the first stage, followed by chemical peeling once every two weeks for 3 times, and oral isotretinoin in the second stage. Finally, oral isotretinoin alone was given in the last stage. While 21 patients in the control group were treated with isotretinoin alone. The treatments lasted for 16 weeks. After each treatment course, the efficacy and adverse effect were compared between the two therapies. The patients in both groups were followed up for 6 months, and the relapse rate and adverse reactions were recorded. Results In the first stage of treatment, effective rates after the second and the third treatment course were 9.09% and 22.73%, respectively, in the sequential group, and 0 and 4.76%, respectively, in the controls. In the second stage, the effective rates after each treatment course were higher in sequential groups than in the controls (1st treatment course: 50.00% vs. 23.81%; 2nd treatment course: 90.90% vs. 42.86%, and the 3rd treatment course: 100% vs. 66.67%. P

For the comprehensive objective assessment of short message service quality, a set of evaluation system of short message service was put forward. A part of the evaluation index was analyzed, and the rationality of related indexes, the development and construction of short message corresponding evaluation system, and the network application were briefly introduced. Through construction and operation of system, the short message service quality evaluation was implemented.

Intensive network management will become the focus in the future. Automation and intelligentization of network management are based on the integration of network management system. Several kinds of technical architectures were discussed to build intensive network management system. A revolutionary way was tried to be found to adjust to the features of network management. The way is to integrate all network management systems into a big cloud named NM-Cloud.

The new security problems in the cloud computing environment were studied based on the characteristics of China Telecom business platform.On the basis of these studies，the security protection framework and construction scheme of cloud computing business platform were put forward.