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Objective To investigate the demands of clinical teaching and perception of the theoretical knowledge and clinical practice among nursing undergraduates at school, and provide reference for establishment of a systematic teaching management system and improve the quality of clinical teaching. Methods Totally 161nursing undergraduates at school were recruited by a convenience sampling method, and an open-ended questionnaire survey was conducted to investigate the nursing undergraduates’ demands of clinical teaching. Results The top-ranked demand for clinical teaching was the combination of theoretic knowledge and clinical practice (91. 93%), followed by the diversified teaching methods including video-assisted and real case teaching (85. 71%) and developing students' interest in clinical work(81. 99%). Most of students were satisfied with clinical teaching. Conclusion Clinical teachers should help students build clinical thinking ability by adopting the teaching method combining theory with practice. Clinical teachers should help students build conference and establish identity and self-worth in clinical practice. (目的 调查在校本科护理学生对临床带教老师的需求, 通过了解学生的理论知识与临床实践认知, 为建立系统、完善的教学管理体系, 不断探索完善临床护理实践与理论知识的紧密结合, 从而为提高临床护理教学质量提供参考。方法 采用便利抽样法对某高校161例在校本科护理学生进行临床教学需求问卷调查。结果 在校生护生对“应将书本理论与临床实操练习相结合”的需求最高(91. 93%), 其次为“教学多样化, 如视频、真实案例等教学”(85. 71%)和“教学过程中应培养学生对临床工作的兴趣”(81. 99%)。满意度调查显示, 156(96. 89%)例在校护生对教学满意。结论 在进行临床教学时, 教师可以通过现场理论与实操教学相结合的方式, 让学生建立自己的临床思维, 老师多分享临床经验, 多鼓励学生, 在潜移默化中帮助学生建立自信心, 帮助护理实习生在临床工作中找到自我价值, 从而提高护理教学质量。)

Objective To investigate the demands for clinical teaching and clinical teachers among nursing undergraduates who participated in their clinical internship, and provide reference for quality improvement of clinical teaching and practice. Methods Totally 179 nursing undergraduates were recruited by a convenience sampling method, and a open-ended questionnaire survey was conducted to investigate nursing undergraduates’ demands for clinical teaching. Results The most-demanded issue of clinical teaching was combination of theory and practice (95. 53%), followed by the clinical experience teaching (88. 27%). Nursing students preferred patience (88. 27%)and encouragement (82. 12%)as key issues in clinical teaching. Conclusion The clinical teaching of theory and practice, particularly in combination, was in high demand among nursing undergraduates. Clinical teachers should pay attention to the combination of theory and practice, and provide more opportunities of independent practice for cultivating students’ clinical thinking abilities. Moreover, clinical teacher should help students build self-confidence and self-esteem, further improving the quality of clinical teaching. (目的 调查临床护理实习生对临床带教老师的需求, 为提高临床护理教学质量提供参考。方法 采用便利抽样法选取某市三级甲等医院179名护理实习生, 对其进行临床教学需求问卷调查。结果 在临床教学中, 护生对理论与实践相结合的需求最高(95. 53%), 其次为临床经验教学(88. 27%), 同时护生更加倾向耐心教学(88. 27%)、和鼓励式教学(82. 12%)。结论 本科护理实习生临床教学总体需求较高, 特别是在理论与实践相结合方面。带教老师可以将现场理论教学和实操教学有机结合, 充分给予学生自主操作锻炼, 帮助学生培养临床思维。同时带教老师应多分享临床经验, 帮助学生建立自信心和实现自身价值, 从而提高护理教学质量。)

Objective To evaluate the emergence agitation and recovery quality of patients with 3 endocrine types of pituitary tumor after transnasal transsphenoidal hypophysectomy. Methods Patients who received pituitary adenoma resection under general anesthesia were enrolled and categorized into three groups: growth hormone group (GH), adrenocorticotropic hormone group (ACTH) and non-functional group (control). Riker Sedation-Agitation Scale (RSAS), the severity of the emergence coughing, headache (by VAS), medical staff satisfaction were recorded in the post-anesthesia care unit. QoR-40 score, headache(by VAS), nausea score (by VRS) and vomiting score, and patient satisfaction were recorded at 24 h postoperatively. Results 163 cases were enrolled in the analysis, including 51 in GH group, 44 in ACTH group and 68 in control group. No significant difference was found regarding the emergence agitation among 3 groups. The ACTH group showed significantly higher nausea VRS score at 24 h after surgery than control group (P＜0.01). The headache VAS score of ACTH group was significantly higher than that in control group (P＜0.001). The incidence of postoperative sore throat in ACTH group was significantly higher than that in control group (P＜0.05). The postoperative QoR-40 score of ACTH group was significantly lower than that of control group (P＜0.01). Conclusions The incidences of postoperative nausea, headache and sore throat are significantly increased and the score of QoR-40 is significantly reduced in patients with ACTH pituitary adenoma.

A water-soluble polysaccharide fraction from root of Potentilla anserine was obtained. Gas chromatogram, FT-IR, physical and chemical characteristics of the Potentilla anserine polysaccharide fraction (PAPF) were analyzed. The protective effects of PAPF against the H2O2 induced process of apoptosis of murine splenic lymphocytes were investigated in vitro. Morphological assessment of apoptosis was performed with light microscope and laser scanning confocal microscope. DNA fragmentation was visualized by agarose gel electrophoresis. The amount of apoptotic cells was measured by flow cytometry. The results showed that PAPF is composed of rhamnose, arabinose glucose and galactose. H2O2 (200 micromol x L(-1)) induced apoptosis of murine splenic lymphocytes with the cell volume reduced, cytoplasm and nuclear shrunk and DNA stained non-uniformly. Condensed chromatin and formation of apoptotic body were observed in the apoptotic cells. Apoptotic bodies in the cells treated with PAPF and H2O2 were less than those in H2O2 treatment alone. DNA fragmentation assay showed that PAPF (50, 100, 200, and 400 microg x mL(-1)) obviously reduced H2O2-induced ladder bands. Flow cytometry analysis showed that H2O2 increased the populations of apoptotic sub-G1 cells from 5.60% (control) to 45.40%, and PAPF decreased H2O2-induced apoptosis to 37.80%, 22.70%, 17.70%, and 8.50%, respectively. In conclusion, PAPF reduced H2O2-induced oxidative damage in a dose dependent manner.