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In this study, 17 amino acids were analyzed by amino acid analyzer in 110 honey samples from three different monofloral honeys, i.e.acacia honey, linden honey and rape honey in China, and the honey variety discrimination was performed based on amino acid content combined with chemometrics. The results showed that the content of hydrolyzed amino acids was higher in rape honey than in acacia and linden honey, and the content of 16 amino acids showed significant differences among the honey varieties. The results of principal component analysis (PCA) revealed that the botanical origins of honey samples clustered according to their amino acid content. The results of least squares discriminant analysis (PLS-DA) showed that rape honey could be distinguished from acacia honey and linden honey. Linear discriminant analysis (LDA) showed that the overall discriminant rate of the three honeys was 92.7%, and the discriminant rate of rape honey was 92.3%. This study would provide data support and reference for the classification and identification of rape honey, acacia honey and linden honey.

The transcription factor activating protein 2α (AP2α) is a nuclear transcription factor that specifically binds to DNA and is involved in the regulation of animal embryonic development, cell growth, apoptosis, tumorigenesis, immunity, and other biological processes. In our previous study, the transcription factor AP2α was discovered as a key disease-resistance candidate gene in the yellow drum, Nibea albiflora, in response to a Vibrio harveyi infection through genome-wide association analysis. In the present study, the AP2α gene, which encodes a protein of 424 amino acids, was cloned from a yellow drum. The N-terminal is a trans-activation domain rich in proline and glutamine (P/Q-rich domain), and the middle is a central basic structure (central basic region), which is a highly conserved helix-span-helix motif responsible for DNA binding and protein dimerization. Multiple alignments of amino acid sequences showed that AP2α was highly conserved, with a homology of more than 84.63% among the detected fish, amphibians, birds, and mammals. Quantitative RT-PCR demonstrated that AP2α was widely distributed in the nine tested tissues, with the highest expression in the blood. Moreover, its transcription was significantly activated in the liver, spleen, and head kidney by V. harveyi infection, especially in the liver wherein the transcript level of AP2α reached a peak at 24 h post infection. Subcellular localization by constructing the recombinant eukaryotic expression plasmid pGEFP-AP2α and transfection into HEK293T cells revealed that AP2α was localized in the nucleus. In addition, the soluble GST-AP2α fusion protein was expressed in Escherichia coli BL21. These results demonstrate that AP2α plays an important role in the immune response against V. harveyi in N. albiflora. We provide new insights into the role of AP2α in the innate immunity of teleost fishes and provide a basis for studies on immune mechanisms and disease-resistant breeding in N. albiflora and other marine fish.

Aiming at the problem of how to give full play to the reverse extrusion ability of bolt to the surrounding rock to make the interface stress balance quickly and enhance the active support effect, we propose the anchorage method of radial pressurization. By adding expansion agent into cement grout to form self-stress slurry, radial expansion stress is generated on the pore wall under the constraint of rock mass, and the stress state of surrounding rock is improved. Under the constraint of thin-walled steel pipe, the radial expansion stress generated by the cement slurry stone body mixed with different amounts of expansion agent is used as a different pressurization variable, and the expansion stress of steel tube wall was measured by static strain gauge and the deformation law of radial stress in stone body with different expansion agent content was analyzed. The finite element software ABAQUS is used to simulate the deformation characteristics of the surrounding rock under different pressurization variables, and the stress field and displacement field of the surrounding rock under stress and deformation are systematically studied. The results show that the radial stress and strength of cement slurry stone with 7% expansion agent are better than those of other proportions, and the compressive stress between cement slurry stone and pore wall increases obviously, resulting in expansion effect. With the increase of radial stress of grout stone body, the compressive stress increases by 65% and the deformation increases by 71% on average, and the radial stress of surrounding rock decreases with the increase of distance from drilling hole.

In recent years, as the third-generation of drugs, new psychoactive substances （NPS） have expanded rapidly and become a serious concern for China’s anti-drug prevention and control system. As a new drug monitoring technology in the current anti-drug field, wastewater analysis is an objective, real-time, accurate, convenient and effective drug monitoring method. In recent years, it has gradually been applied to the monitoring of NPS. This study summarizes wastewater sample collection, target stability research, wastewater sample pretreatment, wastewater sample analysis methods, target NPS consumption calculations and actual monitoring applications, with a view to the construction of a monitoring system for NPS in wastewater, real-time and accurate grasp of information on the use of NPS in cities, the reflection of the actual consumption of different types of NPS and consumption trends in a short period of time, and prediction of the development trend of abused use, which is of great significance for combating NPS crimes, serving and guaranteeing the personal safety of the people, and maintaining social stability.