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Objectives: To study the effect of sex on the clinical outcome of extremely preterm infants (EPIs)/extremely low birth weight infants (ELBWIs) by propensity score matching., Methods: A retrospective analysis was performed for the medical data of 731 EPIs or ELBWIs who were admitted from January 1, 2011 to December 31, 2020. These infants were divided into two groups: male and female. A propensity score matching analysis was performed at a ratio of 1:1. The matching variables included gestational age, birth weight, percentage of withdrawal from active treatment, percentage of small-for-gestational-age infant, percentage of use of pulmonary surfactant, percentage of 1-minute Apgar score ≤3, percentage of mechanical ventilation, duration of mechanical ventilation, percentage of antenatal use of inadequate glucocorticoids, and percentage of hypertensive disorders in pregnancy. The two groups were compared in the incidence rate of main complications during hospitalization and the rate of survival at discharge., Results: Before matching, compared with the female group, the male group had significantly higher incidence rates of neonatal respiratory distress syndrome, bronchopulmonary dysplasia (BPD), severe intraventricular hemorrhage, periventricular leukomalacia, necrotizing enterocolitis, and patent ductus arteriosus ( P <0.05), while after matching, the male group only had a significantly higher incidence rate of BPD than the female group ( P <0.05). There was no significant difference in the rate of survival at discharge between the two groups before and after matching ( P >0.05)., Conclusions: Male EPIs/ELBWIs have a higher risk of BPD than female EPIs/ELBWIs, but male and female EPIs/ELBWIs tend to have similar outcomes.

Objective To investigate the cognitive differences between male and female mice with sepsis-associated encephalopathy (SAE) and its underlying mechanism. Methods The SAE model was induced by caecal ligation and puncture (CLP) in male and female mice aged from 6 to 8 weeks. The cognitive functions of the mice were evaluated by novel object recognition test and contextual fear conditioning test. The expression and distribution of α7 nicotinic acetylcholinergic receptor (α7nAChR) and the proportion of M2 microglia in hippocampus of SAE mice were detected by immunofluorescence staining, and the level of α7nAChR protein was detected by Western blot. Results The cognitive function of male SAE mice was significantly impaired, and the expression level of α7nAChR protein in hippocampus of male SAE mice decreased significantly compared with that of female SAE mice. At the same time, the proportion of M2 microglia in SAE male mice was significantly lower than that in female mice. Conclusion The expression level of α7nAChR protein in hippocampus of SAE male mice is significantly lower than that of SAE female mice, and the proportion of M2 microglia is relatively lower, which lead to the cognitive dysfunction of SAE male mice.

Objective: To explore gender difference in the clinical manifestations of two children with Keishi-Bukuryo-Gan syndrome (KBGS)., Methods: Clinical manifestations of the two children were reviewed. Genetic testing was carried out through next generation sequencing (NGS). Treatment was summarized, and the prognosis was followed up., Results: Both children showed particular appearance including megatooth, abnormal hair distribution, hands' abnormality and language development delay. NGS revealed that both children have carried pathogenic variants of the ANKRD11 gene (c.1903&#95;1907del and c.4911delT), which resulted in shifting of amino acid sequences starting from the Lysine and Proline at positions 635 and 1638, respectively. The female patient exhibited central precocious puberty. Her height has increased by 13 cm, and sex characteristics has retracted after treatment with leuprorelin for 23 months and recombinant human growth hormone for 1 month., Conclusion: Comparison of the two cases with different genders and summary of previously reported cases found that male KBGS patients have more obvious dysmorphisms such as triangular face, synophrys, ocular hypertelorism and vertebral body abnormality, with higher morbidity of epilepsy, mental retardation, autism, congenital heart disease, immune thrombocytopenia and other complications. KBGS is an autosomal dominant disease featuring more evident peculiar appearance and global development delay. Male patients often have multi-system involvement, and multidisciplinary cooperation is required for early recognition of particular features in order to improve the prognosis.

Objective: To analyze the characteristics of modifiable risk factors, coronary artery lesions, reperfusion and drug treatment in young women hospitalized for first acute coronary syndrome (ACS). Methods: Clinical data of young adults of 18 to 44 years hospitalized for first ACS in Beijing Anzhen Hospital between January 2007 and December 2017 were analyzed. A total of 7 106 young adults with ACS were enrolled, 6 593(92.8%) were male and 513(7.2%) were female. There were 2 254(31.7%) patients with ST-segment elevation myocardial infarction (STEMI), 704(9.9%) patients with non-ST-segment elevation myocardial infarction (NSTEMI) and 4 148(58.4%) patients with unstable angina (UA). Clinical characteristics, laboratory examinations, coronary angiography and medical treatment were recorded. The gender differences in the characteristics of modifiable risk factors and coronary artery stenosis in young patients with ACS were also analyzed. Results: Dyslipidemia (87.5%) followed by overweight/obese(83.8%) and smoking (68.5%) were most prevalent modifiable risk factors in men. Dyslipidemia (63.4%), overweight/obese (63.4%) and hypertension (45.5%) were most prevalent modifiable risk factors in women. The prevalence rates of at least 1 risk factor in men and women patients were 99.1% and 87.9%, respectively. Between 2007 and 2017, the rates of hypertension and overweight/obese in men increased, and the rates of dyslipidemia and smoking decreased (trend P <0.01). The changes of incidence of modifiable risk factors through the years in women were not statistically significant. The rate of men receiving coronary angiography was higher than that in women (96.6% vs 93.4%, P <0.01). Women were more likely to suffer left main coronary artery disease than men (7.3% vs 4.1%, P <0.01). Multi-vessel coronary artery disease occurred more in men (43.4%), and single vessel coronary artery disease occurred more in women (47.0%). Women presented with STEMI had a lower probability of receiving clopidogrel/ticagrelor (76.8% vs 86.1%, P <0.01) and angiotensin Ⅰ-converting enzyme inhibitor/angiotonin receptor blocker (ACEI/ARB) (46.5% vs 60.2%, P <0.01) than that in men. Women presented with UA had a lower probability of receiving aspirin (86.3% vs 89.9%), clopidogrel/ticagrelor (69.7% vs 75.6%), statin (78.8% vs 85.0%) and ACEI/ARB (32.7% vs 38.6%) than men (all P <0.01). Women had a higher probability of presenting Killip II-IV than men (38.6% vs 25.6%, P <0.05). Conclusion: The majority of young patients with ACS were men. The prevalence rates of modifiable risk factors are very high in both man and woman. Compared with man, women with AMI are more likely to develop acute heart failure with lower probability of reperfusion and drug treatment.

Objective: To analyze the gender difference in secular trends of body height in Chinese Han adolescents aged 18 years from 1985 to 2014. Methods: Data were extracted from 1985, 1995, 2000, 2005, 2010 and 2014 Chinese National Surveys in Students' Constitution and Health. A total of 124 099 Chinese Han adolescents aged 18 years were included in the analysis. ANOVA test was used to compare the body height between different subgroups stratified by gender and the mean difference and ratio in body height were calculated. Quantile regression model was used to assess the association between body height and gender over time in the adolescents stratified by region or area. Results: The mean body height of adolescents aged 18 years increased significantly from 1985 to 2014 with the rate of 1.3 cm/decade in male adolescents and 0.8 cm/decade in female adolescents, and the highest increment occurred from 1995 to 2005 in both groups. Male adolescents were taller than female adolescents at each subgroup ( P <0.05). Gender difference in body height increased from 11.1 cm in 1985 to 12.6 cm in 2014 and ratio remained to be 1.07-1.08. The body height was taller in adolescents in urban area than in rural area ( P <0.05) and in northeastern China than in other regions ( P <0.05) in both male and female adolescents. The body height of obese male adolescents had been taller since 2000 and the body height of obese female adolescents had been taller since 2010 than other groups. When adjusted for urban-rural areas, region, socioeconomic status and nutritional status, the body height increased over time at P 5 , P 25 , P 50 , P 75 and P 95 in both male and female adolescents, and the increments of gender difference in body height at different percentile ranged from 0.45 cm/decade to 0.57 cm/decade. When stratified by region, the gender difference in body height showed similar trend with total sample except P 5 and P 95 in northeastern China, the difference of increments were not significant. Conclusions: From 1985 to 2014, the gender difference in body height of Chinese Han adolescents aged 18 years increased, and male adolescents showed greater increase than female adolescents. Moreover, the adolescents in northeastern and eastern China had larger increment in body height. It is necessary to paid more attention to the adolescents in central and western China to promote their physical development.

Objective: To explore sex difference in height growth and blood pressure (BP) change among Beijing school-age children and adolescents. Methods: Using physical examination data of 70 769 school-age children and adolescents from primary to high school during 2009-2018 in Shunyi District, a longitudinal dataset was formed with completed anthropometrical measurements of height and blood pressure (BP) after individual information linkage. Age-specific height, BP, growth rate of height and BP as well BP growth rate based on age at peak height velocity (PHA) were calculated. Linear mixed-effects model was used to identify sex disparity in the growth rates of height and BP. Results: Height and BP increased with age in both boys and girls, and the mean height and BP of boys were always higher than those of girls, except age group from 10 to 11 years. Sex disparity existed in growth rates of height and BP ( P <0.001), which was demonstrated by the interaction item of"sex x age"in linear mixed-effects model. The PHA of boys was 12 years old, which was 2 years later than that of girls, about 10 years old. The curves of BP growth rate with age showed double peaks in both boys and girls. Boys reached the peak BP velocity at 13 years old, 3 years lagging behind that of girls who reached the peak at 10 years old. However, the peak of height and BP velocity of boys were higher than that of girls. The change of BP was highly synchronized in time with the increase of height, after adjusting for the growth rate of height by PHA. BP velocity increased with age before onset of puberty till PHA and then declined. Conclusion: Sex disparity in height growth and BP change among school-age children and adolescents is persistent and significant and the change of BP is highly synchronized in time with the increase of height.

Abstract: Objective To analyze the characteristics of the bilateral external ears of Uygur adults by directly observing the morphological characteristics of the external ears of Uygur adults and classifying each feature. The frequency distribution of the characteristics was calculated to provide reference for forensic identification. Methods The 210 cases （75 males and 135 females） of bilateral external ear photos of Uygur adults in Xinjiang that met the inclusion criteria were collected. The frequencies of the features of the external ear were recorded and distinguished between the two sexes and the different sides. The data were statistically analyzed by SPSS 21.0 statistical software. Results The shapes of the external ears of males and females were commonly oblique or rectangular （34.67% of the left external ear of males and 41.33% of the right were oblique; 30.37% of the left and right external ear of females were rectangular）, while triangular ears were the rare variants and the least common. Sex and bilateral differences were observed as regards the form of the helix in the subjects. Normally rolled helix was the most common （58.67% males and 61.48% females for the left ear; 60.00% males and 72.59% females for the right ear）. Wide covering scapha helix was the most rare for the male left ear and flat helix was the most rare for the female right ear. Square and free earlobes were the most common （49.33% males and 62.96% females for the left ear; 40.00% males and 54.81% females for the right ear）, whereas triangular earlobes were rarely seen. Single knob tragus （40.00% males and 37.78% females for the left ear; 37.33% males and 33.33% females for the right ear） and projection type of Darwin's tubercle （50.67% males and 40.00% females for the left ear; 48.00% males and 39.26% females for the right ear） were found to be common. Conclusion The characteristics of the bilateral external ears of male and female Uygur adults have differences, which can be used for forensic identification., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Editorial Department of Journal of Forensic Medicine.)

Objective: To investigate the impact of hypoxic-ischemic brain injury (HIBI) on brain development in neonatal rats of different sexes. Methods: From January 1 to December 31, 2018, 60 7-day-old SD rats were randomly divided into HIBI-F group (20 rats), HIBI-M group (20 rats), and control group (20 rats, 10 females and 10 males). The animal model of HIBI was established with Rice-Vannucci method, with the rats' left common carotid artery double-ligated and severed. The rats were then placed in an incubator and exposed to a hypoxic gas mixture (8% O(2), 92% N(2)) for 90 minutes. No intervention was given to the control group. Two weeks after HIBI, the motor development was evaluated by footprint analysis, the residual brain volume was measured by brain magnetic resonance imaging (MRI), and the damage of synaptic ultra structure was analyzed by transmission electron microscope. One-way ANOVA or χ(2) test was used for inter-group statistical analysis, and paired sample t test was used to compare the bilateral step length and toe distance of rats in the same group. Results: The mortality rate of HIBI-F was significantly higher than that of HIBI-M (20%(4/20) vs. 10%(2/20), χ(2)=40.000, P =0.001). The right step length and toe distance in HIBI-M group and HIBI-F group were significantly shorter than those in control group ((7.5±0.3) cm and (7.9±0.5) cm vs. (8.2±0.5) cm, F= 9.605, P< 0.01, (0.9±0.1) cm and (1.0±0.0) cm vs. (1.1±0.1) cm, F= 71.437, P< 0.01). Besides, according to above data, the right step length and toe distance in HIBI-M group were significantly shorter than those in the HIBI-F group (both P< 0.01). Furthermore, the right step length was significantly shorter than the left step length ((8.3±0.4) and (8.3±0.5) cm, t= 5.289 and 10.580, P= 0.001 and 0.010, respectively) and toe distance ((1.1±0.1) and (1.1±0.1) cm, t= 7.953 and 6.435, respectively, both P <0.01) in both HIBI-M group and HIBI-F group. Similarly, the synaptic gap of the left precentral gyrus neurons was longer in HIBI-M group and HIBI-F group than that in control group ((23.4±1.3) and (19.7±1.6) nm vs. (18.9±0.6) nm, F= 71.719, P <0.01), and also longer in HIBI-M group than that in HIBI-F group ( t= 7.645, P <0.01). Likewise, the residual brain volume in HIBI-M group and HIBI-F group was significantly less than that in control group ((67±4)% and (75±5)% vs. 100%, F= 406.122, P <0.01), and the residual brain volume in HIBI-M group was significantly less than that in HIBI-F group ( t= -5.281, P <0.01). Conclusions: Male neonatal rats are more vulnerable to HIBI and have severer subsequent brain injury and hemiplegia. Different treatment strategies for HIBI patients of different sexes should be developed.

Purpose: To investigate the clinical normal values of juveniles with normal occlusions of She and Han nationality in Lishui area of Zhejiang province, and compare the craniofacial characteristics between She and Han nationality., Methods: Two hundred juveniles of She nationality and 200 juveniles of Han nationality with normal occlusion were recruited. Lateral cephalometric radiographs were taken, and Downs cephalometric analysis was performed. The data were compared using paired samples t test with SPSS 12.0 software package., Results: There was no significant difference between males and females in the values of juveniles of both She and Han nationality. Compared to the male and female juveniles of Han nationality, MP-FH and U1-L1 of the She juveniles increased (P<0.05), while convexity decreased significantly (P<0.05). In addition, L1-MP of She female juveniles decreased significantly in comparison to that of Han female juveniles (P<0.05). There was no significant difference in other values between She and Han nationality., Conclusions: Compared to Han juveniles, She juveniles present an orthognathous and long face with vertical growth pattern. Sex differences are not apparent in juveniles of both She and Han nationality.

Abstract: Objective Logistic regression method was used to establish a multiple regression sex discriminant function to discriminate the complete skull model and the incomplete skull model without frontal bone, occipital bone and mandible of Uygur adults in Turpan, Xinjiang. Methods A total of 117 （60 male and 57 female） three-dimensional skull models were collected by CT. Sixteen cranial measurement indexes were measured and calculated by computer software. The multivariate regression sex discriminant function was established with Logistic regression method and retrospectively tested. Results Among the 16 measurement indexes, except for nose width （ x 7 ） and maximum frontal breadth （ x 13 ）, the remaining 14 indexes had statistical significance of differences between male and female （ P <0.05）. For the discriminant function of complete skull established by eyebrow arch convexity （ x 4 ）, mastoid width （ x 6 ）, maximum cranial length （ x 12 ）, cranial base length （ x 15 ）, cranial circumference （ x 16 ）, the male and female discrimination accuracy was 90.0% and 94.7%, respectively. For the sex discriminant function of incomplete skull without frontal bone established by mandibular angle width （ x 10 ）, mandibular height （ x 11 ） and cranial circumference （ x 16 ）, the discrimination accuracy of male and female was 85.0% and 84.2%, respectively. For the sex discriminant function of incomplete skull without occipital bone established by the index of eyebrow arch convexity （ x 4 ）, the discrimination accuracy of male and female was 80.0% and 73.7%, respectively. For the sex discriminant function of incomplete skull without mandible established by frontal chord （ x 5 ） and occipital protrusion angle （ x 9 ）, the discrimination accuracy of male and female was 85.0% and 78.9%, respectively. Conclusion The computer software and system developed in our study can achieve sex discrimination of complete skulls and incomplete skulls without frontal bone, occipital bone or mandible., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Editorial Department of Journal of Forensic Medicine.)

Objectives: The aim of the study was to determine whether there was gender difference in clinical manifestations and comorbidities in the patients with Spondyloarthritis (SpA) in China. Methods: 346 patients fulfilling ASAS criteria for SpA were recruited from the Third Affiliated Hospital of Sun Yat-sen University, including 280 males and 66 females. A comparison was conducted in terms of age at onset, disease course, family history, HLA-B27 positivity, clinical manifestations, erythrocyte sedimentation rate (ESR), and C-reactive protein (CRP), the bath ankylosing spondylitis disease activity index (BASDAI) and AS disease activity score (ASDAS), and comorbidities between male and female patients. Results: Compared with female patients, male patients were younger at disease onset (22±7 vs 27±9, P< 0.001),had higher rates of morning stiffness (74.3%), and higher scores of CRP and ASDAS-CRP ( P< 0.010, P= 0.014). However, no significant gender difference was observed in other clinical parameters like clinical manifestations, family history, HLA-B27 positivity, BASDAI, and BASFI and treatment. Male SpA patients had a higher prevalence of Hepatitis B virus (HBV) infection (26.2%) than that of female patients (8.3%), and a higher prevalence of osteoporosis (30.5% vs 14.3%, P< 0.01), especially with a lower lumbar T score. Logistic regression analysis reviewed that limited weight ( OR= 0.94, P< 0.001), high ASDAS-CRP ( OR= 1.58, P= 0.006) and male ( OR= 8.02, P= 0.004) are more inclined to have osteoporosis. Conclusion: Compared with female patients, male patients were younger at disease onset and higher scores of CRP and ASDAS-CRP. No significant gender difference was observed in clinical manifestations, family history, HLA-B27 positivity, BASDAI, and BASFI and treatment. Male SpA patients had a higher prevalence of HBV infection and osteoporosis than female patients. Comorbidities should be paid more attention in the patients with SpA.

Objective: Ververck index (VI) reflects thoracic development, body type, and nutritional status. This study aimed to investigate the VI of singleton neonates with a gestational age (GA) of 27-42 weeks at birth, and to establish percentile curves of VI of the neonates., Methods: Cross-sectional cluster sampling was performed between April 2013 and September 2015. Body weight, body length, and chest circumference were measured for 16 865 singleton neonates with a GA of 27-42 weeks in two hospitals in Shenzhen, China. VI was calculated and the percentile curves of VI were plotted for the neonates., Results: Mean VIs were obtained for singleton neonates with a gestational age of 27-42 weeks (in three groups of male, female, and both sexes), and related 3rd-97th percentile curves were plotted. As for the 50th percentile curve, the singleton neonates with a GA of 27 weeks had the lowest 50th percentile value of VI, which gradually increased with the increase in GA. The singleton neonates with a GA of 42 weeks had the highest 50th percentile value of VI. Girls had a slightly higher 50th percentile value of VI than boys in all GA groups., Conclusions: VI of neonates increases with the increase in GA. Female neonates may have a slightly better thoracic development, body type, and nutritional status than male neonates at birth. The percentile curves of VI plotted for singleton neonates with a GA of 27-42 weeks (in three groups of male, female, and both sexes) can provide a basis for evaluating thoracic development, body type, and nutritional status of neonates at birth in Shenzhen, China.

Objective: To analyze the level of hemoglobin level and the prevalence of anemia in students aged 7-14 years in China. Methods: According to the 2010 Chinese National Surveys on Students'Constitution and Health, 71 130 students who were aged 7, 9, 12 and 14 years and had received hemoglobin test were sampled as the subjects, WHO anemia diagnostic criteria was used to explore the hemoglobin level and the prevalence of anemia. Results: The mean concentrations of hemoglobin were (129.25±11.37) g/L, (131.04±11.08) g/L, (135.02±12.12) g/L and (138.47±13.97) g/L and the prevalence of anemia was 9.0 % , 6.5 % , 8.6 % and 7.7 % respectively in 7, 9, 12 and 14 years old students. The prevalence of anemia was 9.7 % in girls, significant higher than that in boys (6.2 % ), and 8.8 % in rural students, significantly higher than that in urban students (7.1 % ). The hemoglobin levels were (133.19±12.56) g/L, (133.28±12.76) g/L, (134.24±12.73) g/L and (133.79±12.16) g/L and the prevalence of anemia were 8.8 % , 8.3 % , 6.7 % and 5.9 % respectively in thin, normal, overweight and fat students, there were significant differences among four groups. Conclusion: The prevalence of anemia in students aged 7-14 years in China was still high, and there was significant differences among different gender, area, age and nutritional status groups.

To investigate the sexual morphological differences and female individual fecundity of Saurogobio dabryi, the sexual size dimorphism, sex ratio and female individual fecundity of 76 individuals collected from the lower reaches of the Jialing River (Hechuan section) in breeding season were analyzed. The results showed that the sex ratio of S. dabryi in the downstream of Jialing River was 1:1 in the breeding season, and no significant difference in the body size between males and females. However, some local morphological traits (such as cephalosome and the truck) exhibited significant difference between males and females, for example, the head, the pectoral fin and the pelvic fin were significantly larger in males than in females, while the width, height and length of trunk exhibited the opposite results, all which were related to the sexual selection. A principal component analysis showed that the first three components accounted for 75.2% of variations in the morphological variables. However, all traits overlapped between males and females, thus they could not be separated by morphological characters. By establishing discrimination function, 92.1% samples could be distinguished. The individual fecundity of S. dabryi ranged from 979 to 19979 eggs, and exhibited significant positive relationship with body length and carcass mass. Compared with the historical data, the fecundities of S. dabryi increased significantly in the present study, which should be the active adaption of the species to the currently depleting population, as well as to the changing of water ecological environment.

Objective: To understand the prevalence of diabetes in adults in Hebei province. Methods: The survey was conducted among the local residents aged ≥18 years, who were selected through multi stage cluster sampling from September 2013 to April 2014. Design-based methods were used to analyze the prevalence in the last 12 months. Results: A total of 7 678 adults were included in the study. The overall prevalence of diabetes in Hebei was 12.9% (13.1% in men and 12.8% in women), there was no sex specific difference in diabetes prevalence ( P =0.09). The prevalence was higher in urban residents (16.2%) than that in rural residents (12.1%), the difference was significant ( P <0.01). The prevalence of diabetes increased with age ( P <0.01). The prevalence of impaired fasting glucose was 5.1%, the difference was significant between men and women and between rural area and urban area ( P <0.01). The prevalence of impaired glucose tolerance was 10.4%. The difference of prevalence of impaired glucose tolerance in men and women and between rural area and urban area was not significant. The rates of awareness, treatment and control of diabetes were 50.7%, 46.3% and 37.5%, respectively. Women had higher awareness rate and treatment rate of diabetes than men, the difference was significant ( P <0.01). Conclusions: The prevalence of diabetes was high in adults in Hebei province, the difference in diabetes prevalence between urban area and rural area was significant. The prevalence of impaired fasting glucose and impaired glucose tolerance were high, but the rates of awareness, treatment and control of diabetes were low.

Objective: To measure the maximal capacity of students aged 13- 15 years by graded exercise test, and establish a model to indirect predict VO2 max., Methods: A total of 31 school-aged students in Wuhan participated in the study. Maximal oxygen uptake was obtained from a graded maximal exercise test, the anthropometric variables including height, weight, vital capacity( VC), resting heart rate, fat mass were measured and body mass index( BMI), body fat percentage( FAT%) was calculated. The relationships between maximal oxygen uptake and anthropometric characteristics were investigated in partial correlation analysis and a linear multiple regression model was established., Results: There was a significant difference in mass-relative VO&#95;(2max)[( 48. 00± 5. 80) L / min vs( 39. 79 ± 6. 37) L / min, P < 0. 001], absolute VO&#95;(2max)[( 2. 66 ± 0. 29)m L /( kg·min) vs( 2. 02 ± 0. 36) m L /( kg·min), P < 0. 001], pulse oxygen [( 13. 33 ±1. 28) m L / beat vs( 10. 34 ± 1. 70) m L / beat, P < 0. 001] between the boys and girls respectively. And a statistically significant negative correlation was observed between values of maximal oxygen uptake and weight, BMI, FAT% and resting heart rate( P <0. 05), while lean body mass was positive with VO&#95;(2max)( P < 0. 05). Multiple regression analysis suggested that the best VO&#95;(2max) predictive model of boys was VO&#95;(2max)= 0. 856 +0. 044 × lean body mass- 0. 011 × resting heart rate + 0. 0002 × vital capacity( R =0. 903, R~2= 0. 816), and the prediction equation of girls was follows: VO&#95;(2max)= 4. 769 +0. 044 × lean body mass- 0. 020 × resting heart rate- 0. 254 × age( R = 0. 813, R~2=0. 662)., Conclusion: The present study suggests that anthropometric characteristics might be closely related with maximal aerobic capacity and can effectively predict the maximal aerobic power.

To explore the sexual dimorphism of Tanichthys albonubes and its relationship with swimming performances, the morphological characteristics of fins and trunks of male and female T. albonubes were measured and analyzed by using multivariate statistical analysis methods, aiming to explore the difference and its mechanism of swimming performance between male and female under different selected pressure in long-term evolution period and then, providing meaningful information for the protection of T. albonubes. The results showed that body lengths, head depths and breadths, caudal fin areas, distances of snout tip to occipital bone terminus and ventral fin origin to dorsal fin terminus had no significant difference between male and female T. albonubes. The females had significantly higher values in head depths, body breadths, distances of ventral fin origin to dorsal fin origin, snout tip to dorsal fin and to anal fin origin, occipital bone terminus to dorsal fin origin than those in the males. However, head lengths, pectoral fin, dorsal fin, ventral fin and anal fin areas were significantly lower in the females. The results of principal component analysis showed that the contribution rate of the first principal component (PC1) was 74.2%. Obviously loading factors were body lengths, depths and breadths, head lengths, breadths and depths and the distances of each fin mainly reflecting body overall characteristic parameters of T. albonubes. For the second principal component (PC2), the contribution rate was 15.7% and the obviously loading factors were pectoral fin, dorsal fin, ventral fin and anal fin areas which mainly reflected the characteristic parameters of fins. The gender identification of T. albonubes was indistinguishable on PC1, but could be obviously distinguished from PC2. Accuracy rates of sexual discriminant equation which was established by pectoral fin, dorsal fin, ventral fin and anal fin areas and body breadths were 91.8%-92.5%. The results of swimming performance showed that burst swimming speeds (U burst ) had no significant difference between male and female T. albonubes. However, the critical swimming speeds (U crit ) were significantly decreased in the females than in the males. The findings indicated that the sexual dimorphism of T. albonubes was mainly concentrated on fin characteristics associating with swimming performance. Also having lower U crit due to its lower pectoral fins areas than the male, the female T. albonubes had longer hindquarters cadres to ensure high U burst which facilitated them to avoid predators and other emergency events in volatile streams. Larger fin areas than in the female caused higher U cirt in the male contributing to chasing female in breeding period and other persistent exercises.

Objective: To investigate the features of methylation in the promoter region of glucose-6-phosphate dehydrogenase (G6PD) gene and the association between gene promoter methylation and G6PD deficiency., Methods: Fluorescent quantitative PCR was used to measure the mRNA expression of G6PD in 130 children with G6PD deficiency. Sixty-five children without G6PD deficiency served as the control group. The methylation-sensitive high-resolution melting curve analysis and bisulfite PCR sequencing were used to analyze gene promoter methylation in 22 children with G6PD deficiency and low G6PD mRNA expression. The G6PD gene promoter methylation was analyzed in 44 girls with normal G6PD mRNA expression (7 from G6PD deficiency group and 37 from control group)., Results: Twenty-two (16.9%) children with G6PD deficiency had relatively low mRNA expression of G6PD; among whom, 16 boys showed no methylation, and 6 girls showed partial methylation. Among the 44 girls with normal G6PD mRNA expression, 40 showed partial methylation, and 4 showed no methylation (1 case in the G6PD group and 3 cases in the control group)., Conclusions: Gene promoter methylation is not associated with G6PD deficiency in boys. Girls have partial methylation or no methylation in the G6PD gene, suggesting that the methylation may be related to G6PD deficiency in girls.

Objective: To investigate the development of nocturnal sleep pattern in infants., Methods: Fifty healthy full-term newborns born in Chongqing Maternal and Child Health Care Hospital were chosen for a prospective longitudinal study. A non-invasive sleep monitor, Actiwatch, was used to monitor infants' 12 sleep parameters on the 10th day, 28th day, the first Tuesday at the 2nd, 3rd, 4th, 5th, 6th, 9th and 12th month after birth, each monitoring time lasting 60 hours. All sleep parameters were analyzed by two-level mixed effect model., Results: Twenty-two boys and 25 girls completed the whole follow-up study. From birth to the 12th month after birth, the nocturnal sleep onset latency (NSOL) decreased by about 48% at 3 months of age and by 83% at 6 months of age. The nocturnal sleep efficiency (NSE%) increased from 66% to 87%, the nocturnal total sleep time (NTST) increased from 416 minutes to 517 minutes, and the longest nocturnal continuous sleeping time (L-NCST) increased from 197 minutes to 327 minutes. NSE%, NTST and L-NCST increased with age (P<0.01). The 3rd to 12th month ratios of NSE%, NTST and L-NCST were 86%, 84% and 72%, respectively, and the 6th to 12th month ratios of those were 97%, 91% and 94%, respectively. The nocturnal total wake time (NTWT) and longest nocturnal continuous waking times (L-NCWT) decreased with age (P<0.01). The decline speeds in the first half year were 5-6 times of those in the second half year after birth (P<0.05). NTST, nocturnal continuous sleeping ability and NSE% in boys were lower than those in girls (P<0.05)., Conclusions: Infantile nocturnal sleep patterns develop rapidly during the first 6 months, especially within the first 3 months after birth. Partial infantile sleep parameters are related to gender.

Objective: To explore the value of estimating chronologic age based on the grades of mandibular third molar development. To evaluate whether mandibular third molar could be used as an indicator for estimating the age under or over 18 years., Methods: The mineralization status of mandibular third molar of 1 845 individuals aged 10 - 30 was graded and marked based on Demirjian's classification of grades reformed by Orhan. Gender difference was examined by t-test. A cubic regression model was established to analyze the correlation between third molar and chronologic age. Each grade of age cumulative distribution diagram and ROC curve was respectively performed to evaluate the relationship between third molar and the age of 18. Using Bayes discriminant analysis, an equation was established for estimating the age of 18., Results: The inner-rater reliability was 0.903. Statistical analysis showed a moderate correlation between age and grade. Significant differences of both genders were found only in grade D and H (P < 0.05). Males at the grades from 1 to D and females at the grades from 1 to C were under 18 years old, and both males and females at grade H were over 18 years old. The area under the ROC curve was 0.797 (P < 0.05)., Conclusion: Third molar development shows a high correlation with age, and combined with other indicators, it can be used to estimate the age of 18.

Objective: To establish height-weight indices in newborns of different gestational ages and to provide reference data for evaluation of intrauterine fetal growth., Methods: The weight, height, crown-rump length, head circumference, and chest circumference of 8 357 newborns were measured in Maternal and Child Health Care Hospital of Bao'an District of shenzhen between 2005 and 2006, with the method of fact-finding investigation with cross-sectional cluster sampling, to establish the height-weight indices in newborns of different gestational ages., Results: Five gender-specific height-weight indices (Quetelet Index, QI; Kaup Index, KI; Rohrer Index, RI; Livi Index, LI; Polock Index, PI) in newborns of different gestational ages (28-44 weeks of gestation) in three different groups (boys+girls, boys, and girls) were established in Shenzhen, China, and were expressed as mean gestational weeks±SD. The five indices above all increased with increasing gestational age, and the highest values appeared at 41-43 gestational weeks, suggesting that body density and enrichment degree increased constantly with increasing gestational age. Three indices (QI, KI and PI) were higher in boys than in girls (P<0.05)., Conclusions: With the increasing gestational age, the body density and enrichment degree of newborns increase, and the enrichment degree in boys is better than that in girls.

Pseudoallergic reactions occured after the first administration of patients, and the pathogenic mechanisms of them were different from the allergic reactions which needed excitation after antigen sensitization. To provide a basis for evaluation, clinical use and drug development of pseudoallergic reactions, the models were established by two kinds of Chinese herbal injections (CHI) both on different strain or gender mice. With the use of ICR, Kunming, BALB/C, C57 mice, pseudoallergic tests of two CHI were conducted to compare the sensitivity of four strains mice, and compared the differences in male and female animals. Test substances contain 0.8% Evans blue (EB) were intravenously injected into different strain and gender mice. Scores of ear blue staining and quantitation of ear EB exudation were the parameters for pseudoallergic reaction. Results of strain difference indicated that both CHI A and B could cause severe pseudoallergic reactions indicated by obvious vascular hyperpermeability on ICR mice. The pseudoallergic reactions in ICR mice are more obvious under the the same dose of injection, which stated the sensibility of ICR mice. And the reactions of KM mice and BALB/C mice were slightly reduced which compared to ICR mice, even alomost nothing on C57 mice. Comparison results of gender difference showed that one CHI was not have significant difference in male and female animals, but male animals were more susceptible than females on another CHI. Therefore, ICR mice were preferable experimental strain on the model of pseudoallergic reactions induced by CHI A and B. Because of female animals were easily influenced by estrous cycle, the pseudoallergic reactions induced by CHI A and B select and use male mice befittingly.

Objective: To investigate incidence and clinical features of multiple organ involvement in Chinese patients with Fabry disease., Methods: We collected 151 patients of 31 families with Fabry disease, all of whom were confirmed by classic pathology, decreased α-galactosidase A activity or GLA mutation from the year of 2011 to 2014 in Department of Neurology, Peking University First Hospital. The clinical data included incidence and onset of neuralgia, renal dysfunction, heart disease, hypertension and cerebral stroke. We calculate the incidence of these symptoms and analyze their gender difference using chi-square with SPSS 17.0 software., Results: Among 31 families with Fabry disease, 151 patients (82 males and 69 females) were affected, including 91 patients (60.26%) with neuralgia, 62 patients (41.06%) with renal disease, 33 patients (21.85%) with heart disease, 33 patients (21.85%) with hypertension, and 21 patients (13.91%) with cerebral stroke. The peak incidence of neuralgia was from 10 to 20 years old, followed by renal disease from 30 to 40 years old, heart disease and stroke from 40 to 50 years old. There is no peak period for hypertension. Except for males with higher incidence of renal dysfunction than females (P < 0.05), there were no gender difference in the incidence of other organ involvement., Conclusion: The incidence and onset of multiple organ dysfunction in Chinese patients with Fabry disease displayed special regularity. Gender difference appeared only in renal disease.

Objective: To explore the association of adverse childhood events with early puberty timing and possible gender differences., Methods: Data was gathered through questionnaires, physical and secondary sexual characteristics, examination with breast stage in girls and testicular volume in boys measured under informed consent among children in grade 3 to grade 5 from a large-scale primary school. Information regarding adverse childhood experiences (ACEs), time of screening and physical activity was included in the questionnaire. Age limits on secondary sexual characteristics for defining early puberty established under the "China Puberty Research Collaboration Project" were used to classify early puberty timing. Body mass index was calculated and used to classify both overweight and obesity, in each gender., Results: Among the 1 744 children aged 8.2-12.2 years old (957 boys), the prevalence rates of early puberty timing among boys and girls were 7.5% and 14.6%, respectively, with gender differences (χ² = 11.671, P < 0.001). Boys who reported having experienced serious adverse family events and girls with physical abuse were more likely to develop early puberty. Results from multivariate logistic regression analysis showed that family life events were associated with a higher risk of early puberty timing in boys (odds ratio: 2.531, 95% CI: 1.276-5.020) while experience related to physical abuse appeared a risk factor of early puberty timing in girls (odds ratio: 2.453, 95% CI: 1.588-3.788)., Conclusion: Physical abuse and adverse family life events seemed to be associated with early puberty timing, suggesting further longitudinal study should be carried out to understand the nature of these findings and gender differences.

Background: Medical technology has transformed the body image of women and altered perceptions of beauty and sexual attraction. While "sexual attraction" is a fundamental concept in sexology, the characteristics of this concept have not been studied in the field of nursing. Because nurses provide advice and health education for women, it is essential to clarify the concept of sexual attraction for the benefit of related nursing research and for the further development of nursing knowledge., Purpose: This study explores the concept of sexual attraction in a Taiwanese social context using concept analysis based on an evolutionary perspective., Methods: Inductive inquiry is used to compare and contrast articles from the academic literature, magazines, and newspapers, and data from participant observation and interviews are used to generate exemplars. The process by which the concept of sexual attraction has evolved over time is captured from three distinct aspects: significance, use, and application., Results: The definitional statement of sexual attraction includes the five dimensions of: 1. sexual-oriented psychological dynamics; 2. personal aesthetics and sensory experience; 3. instinct body forces; 4. body language of self; and 5. social and cultural norms., Conclusions / Implications for Practice: This study scrutinized the changes in attributes that emphasize the biological, objectified body, and stereotyped gender roles of women. Further directions for research and nursing knowledge development are suggested. Examples include identifying the changes in the concept of sexual attraction that result from technological advancement and further clarifying the experiential knowledge of sexual attraction that represents the selfhood and independence of women in Taiwan.

Objective: To understand the trend and current characteristics of children engaged in road traffic injury. Data was from the Nation Injury Surveillance System (NISS) and the results of the study would provide basis for corresponding intervention strategies and decision-making., Methods: Descriptive analysis was applied to display the trend of child road traffic injury from 2006 and 2013 and also to depict the general information, injuries event and clinical characteristics of child road traffic injuries in 2013., Results: The number of child road traffic injuries increased from 2006 to 2013 and ranking the second cause of child unintentional injuries during these years. However, the proportion of child road traffic injuries among child unintentional injuries decreased in the same period. In 2013, sex ratio appeared as 1.82, with 17- year-old age group accounting for 10.86% . Injuries caused by motor traffic vehicles accounted for 66.44% , with July/August, weekends and 17-18 PM as the peak period or time. Transportation related injuries accounted for 65.42% , with 73.53% as bruise. 33.81% of the injury involved in the head but 76.42% of injuries were minor, while 74.86% went home after the treatment., Conclusion: Child road traffic injury should not be ignored. Age and sex differences should be taken into account when carrying out education programs on child road traffic safety. Male students at senior high school or preschool were target groups when carrying out child road traffic injury intervention programs.

Objective: To establish the multiple risk factors models for patients with acute coronary syndromes (ACS) of different genders and quantitatively assess the pathopoiesis of all factors., Methods: A total of 2 308 consecutive ACS inpatients and a control group of 256 cases with normal coronary artery from January 2010 to December 2012 were enrolled and divided into 4 groups of female ACS (n = 970), male ACS (n = 1 338), female control (n = 136) and male control (n = 120). All demographic and clinical data were collected by the physicians and master degree candidates in the division of cardiology., Results: The Logistic regression models of multiple risk factors were established for ACS by different genders. More than 45 years of age, dyslipidemia, type 2 diabetes mellitus, obesity and hypertension were all independent risk factors of ACS for different genders (P < 0.05). However, the same risk factors had different pathogenic effects on ACS between genders. The odds ratio (OR) was markedly different for females and males: per 5-year increase aged over 45 years (1.45 vs 1.13), dyslipidemia (3.45 vs 1.68), type 2 diabetes mellitus (4.06 vs 2.33), obesity (2.93 vs 1.91) and hypertension (1.78 vs 3.80) respectively (all P < 0.05). In addition, current smoking increased the risk of ACS attack in males by 5.49 (P < 0.05) while not statistically significant in females. Particularly cerebral ischemic stroke increased the risk of ACS attack by 5.49 folds in males other than females (P < 0.05)., Conclusion: Type 2 diabetes mellitus, dyslipidemia and obesity may present higher risks of ACS attack for females than males. And smoking and hypertension are much more dangerous for males. Males with cerebral infarction are more susceptible for ACS than females.

Objective: To investigate the influencing factors for lymphocyte subsets in children 0 to 6 years of age., Methods: Umbilical artery blood samples from 45 healthy full-term infants and venous blood samples from 79 healthy children between 0 and 6 years were collected. According to the methods of delivery, the full-term infants were divided into vaginal delivery group (n=22) and cesarean section group (n=23). Healthy children were divided into different age groups: 28 days to 12 months (n=25), 1-3 years (n=26), and 3-6 years (n=28). Lymphocyte subsets were examined by flow cytometry. The influencing factors including delivery method, sex, and age, which might have an effect on the lymphocyte subsets, were analyzed., Results: There were significant differences in T and Ts cell counts, percentage of B cells, and percentage and count of natural killer (NK) cells between the full-term infants of vaginal delivery and cesarean section (P<0.05). The absolute counts and percentages of different lymphocyte subsets showed no significant differences between males and females in healthy children (P>0.05). The counts of all lymphocyte subsets except Ts and NK cells varied significantly between different age groups (P<0.05)., Conclusions: Lymphocyte subsets in children under 6 years of age are more profoundly affected by age. Delivery method is also a contributing factor in newborn infants. The reference range of lymphocyte subsets in children should be established for different age groups.

Total knee arthroplasty (TKA) has been successfully applied for the treatment of the knee pathology at the end stage such as osteoarthritis and rheumatoid arthritis. Although TKA has became a very mature technology, some patients still suffer from the persistent pain after surgery. The cause of this pain have been recognized as the operation or technical error in most cases, and it usually requires a surgical intervention. Only when the cause of pain is judged accurately, can the operation and other factors be estimated correctly, determining the appropriate treatment methods. In the article, the causes of the post-operative pain after TKA are reviewed, which may be helpful to study the causes of the pain, and to decrease the occurrence incidence of pain.

Objective: To investigate the prevalence of hypertension and the relationship between hypertension and obesity in children and adolescents aged 6-18 years in Nanning, Guangxi Province, China., Methods: A stratified cluster random sampling method was adopted in Nanning to select 7 893 children and adolescents aged 6-18 years as research subjects. Questionnaire surveys were conducted, and blood pressure, height, weight, and other indicators of growth and development were measured., Results: An increasing trend with age for both systolic blood pressure (SBP) and diastolic blood pressure (DBP) was observed. Detection rates of hypertension, high SBP (HSBP) and high DBP (HDBP) were 6.58%, 4.02% and 3.81%, respectively. The detection rates of hypertension and HSBP in boys were significantly higher than in girls (P&lt;0.05). The detection rates of hypertension in normal, overweight and obesity groups were 3.87%, 9.84% and 19.23%, respectively. The obesity group showed the highest detection rates for hypertension, HSBP and HDBP, followed by the overweight group and normal group. Compared with that in the normal group, the odds ratios (95% CI) for hypertension in the overweight and obesity groups were 2.71 (1.69-5.96) and 5.91 (3.46-7.63), respectively. Blood pressure showed a positive correlation with age, height, weight and BMI (P&lt;0.01)., Conclusions: The present study provides with the current information and characteristics of blood pressure of children and adolescents aged 6-18 years in Nanning, Guangxi. Blood pressure is correlated with gender, age, height, weight and BMI. Obesity is positively correlated with the prevalence of hypertension and the risk of hypertension increases with body weight.

Objective: To explore the RET reference values of newborns in Beijing Area and the relationship between the RET and the day ages of newborns., Methods: 2 759 (1 404 males and 1 355 females) healthy newborns (born in Beijing Tongren Hospital from January 2007 to December 2012 with 4.31 average day age) were selected, the RET percentage was counted with XE-2100 (adjusted before in use), after the inverse sine transformation of variable's square root, Statistical results show as X⁻ ± s, the reference value refers to bilateral X⁻ ± 1.96 s. The sample was divided into nine groups according to different day ages. Independent sample t served to test and compare the RET difference between two genders newborns, single-factor analysis of variance served to compare the RET difference of the different day ages newborns'groups, Bonferroni adjustment method served to apply the pairwise comparisons. SPSS 18 was used for statistical analysis between males and females across nine age groups., Results: The normal RET reference values of male and female newborns separately were 2.408%-3.438%, 2.740%-3.912%. RET of male newborns was lower than that of female newborns, the difference was statistically significant (t = -2.564, P < 0.05). The RET difference of different day ages of male and female newborns was statistically significant (F = 138.105, P < 0.01). The comparison of the same day newborns proves that there is RET difference between the male, female newborns of fifth day groups, respectively (2.656 ± 0.067)%, (3.295 ± 0.149)%, there is statistically significant (t = -2.219, P < 0.05) . Newborns' RET vary with day ages, and there is obvious peak value., Conclusions: There is RET difference between the male and female newborns, which vary with day ages. The study on the RET reference values of newborns of different genders or different day ages contributes to the clinical disease diagnosis and treatment as reference.

Objective: To explore the correlation between the expression of phosphorylated histone H2AX (γ-H2AX) and Epstein-Barr virus (EBV) infection in nasopharyngeal carcinoma (NPC) specimens, and to investigate the role and mechanism of EBV-induced DNA damage in NPC tumorigenesis and development in vitro., Methods: We enrolled 50 cases of NPC and 20 cases of nasopharyngitis (NPI) specimens to test the expressions of γ-H2AX and EBV latent membrane protein 1 (LMP1) by immunohistochemical method (IHC). And then in LMP1-negative samples, we detected the EBV-encoded RNA (EBER) using in situ hybridization. The γ-H2AX level was detected in NPC cells CNE1 before and after EBV infection using Western blotting., Results: γ-H2AX was expressed in most NPC specimens (94%), which was much higher than that in NPI (40%), and EBV was presented in 94% of NPC but only 30% in NPI. Moreover, γ-H2AX was positive in 97.9% of the EBV-positive specimens, which indicated the close correlation between γ-H2AX expression and EBV infection (P<0.05). Finally, Western blotting showed that γ-H2AX level significantly increased in CNE1 cells after EBV infection., Conclusion: This study demonstrated that an intimate connection existed between γ-H2AX expression and EBV infection in NPC both in vivo and in vitro. EBV infection might induce DNA damage in CNE1 cells, which causes genome instability and initiates or promotes the tumorigenesis and development of NPC.

Objective: To explore the percentage of CD4(+);CD25(+);Foxp3(+); regulatory T cells (Treg) in peripheral blood of chronic lymphocytic leukemia (CLL) patients and the correlations with clinical prognosis., Methods: The study enrolled 30 healthy individuals and 28 CLL patients. The CD4(+);CD25(+); Treg and CD4(+);CD25(+);Foxp3(+); Treg were detected by the flow cytometry in their peripheral blood. Of the 28 CLL patients, 19 received treatment and follow-up., Results: The number of CD4(+);CD25(+); Treg in the pre-treated cases (n = 28) was higher than that in the healthy controls (n = 30) with significant statistical difference (P < 0.05). The number of CD4(+);CD25(+);Foxp3(+); Treg was higher in the pre-treated cases (n = 28) than that in the treated cases (n = 19) and in the healthy controls (n = 30) (P < 0.05). Compared with the healthy controls, the treated cases (n = 19) had the higher level of CD4(+);CD25(+);Foxp3(+); Treg (P < 0.05). The CD4(+);CD25(+);Foxp3(+); Treg was positively correlated with the expressions of CD38, β2-microglobulin (β(2);-MG), zeta-associated protein 70(ZAP-70) and the clinical Binet and Rai stages., Conclusion: The CD4(+);CD25(+);Foxp3(+); Treg might be a valuable indicator for assessing the therapeutic efficacy, disease progression and prognosis of the CLL patients.

In this study,spectra analysis was conducted to investigate the sexual dichromatism and relationship between body color and quality of the red-eared slider turtle (Trachemys scripta). Visual modeling revealed that the differences of the hues of body colors' ultraviolet (UV) components between male and female were significant. Moreover, the visible(VIS)components of the hues of forelimbs were correlated with body qualities of male but not of female. Our results indicated that UV might act as a signal for sexual cognition in red-eared slider turtles, and female might evaluate male by the VIS components of the hues of forelimbs. These findings provided preliminary results and perspectives to the sexual dichromatism and sexual selection behaviors of red-eared slider turtles.

Objective: To establish the mathematical models of stature estimation for Sichuan Han female with measurement of lumbar vertebrae by X-ray to provide essential data for forensic anthropology research., Methods: The samples, 206 Sichuan Han females, were divided into three groups including group A, B and C according to the ages. Group A (206 samples) consisted of all ages, group B (116 samples) were 20-45 years old and 90 samples over 45 years old were group C. All the samples were examined lumbar vertebrae through CR technology, including the parameters of five centrums (L1-L5) as anterior border, posterior border and central heights (x1-x15), total central height of lumbar spine (x16), and the real height of every sample. The linear regression analysis was produced using the parameters to establish the mathematical models of stature estimation. Sixty-two trained subjects were tested to verify the accuracy of the mathematical models., Results: The established mathematical models by hypothesis test of linear regression equation model were statistically significant (P<0.05). The standard errors of the equation were 2.982-5.004 cm, while correlation coefficients were 0.370-0.779 and multiple correlation coefficients were 0.533-0.834. The return tests of the highest correlation coefficient and multiple correlation coefficient of each group showed that the highest accuracy of the multiple regression equation, y = 100.33 + 1.489 x3 - 0.548 x6 + 0.772 x9 + 0.058 x12 + 0.645 x15, in group A were 80.6% (+/- lSE) and 100% (+/- 2SE)., Conclusion: The established mathematical models in this study could be applied for the stature estimation for Sichuan Han females.

Objective: To discuss bone development trend in the knee joint of Tibetan teenagers in Sichuan province and to effectively update the database for estimating the living age of Tibetan teenagers in terms of bone age of the knee joint., Methods: Radiographs including epiphysis of distal femur, proximal tibia and proximal fibula were taken from 483 Tibetan male and female teenagers aged from 14 to 19 years old in Aba prefecture of Sichuan province in order to observe epiphyseal growth situation. The descriptive data of the epiphyseal closure ages of these teenagers' knee joints were statistically analyzed by SPSS 16.0 software., Results: The distal femur epiphyseal closure occurred earliest, while the proximal fibula epiphyseal closure occurred latest. The epiphyseal growth of knee joints of females occurred about one year earlier than that of males., Conclusion: The forensic information and data related to Tibetan teenagers' bone age identification should be updated regularly. These results provide potential value for the practice of forensic medicine, anthropology and clinical medicine.

Objective: To understand and analyze the morphological characteristics of hyoid bone and its gender difference in order to find out its forensic significance., Methods: The hyoid bones of 68 adult corpses were dissected from redundant soft tissues after heating. The connection status of body of hyoid, greater cornu and lesser cornu, the morphological characteristics of hyoid bone and the degree of ossification were observed by visual inspection. The height of hyoid bone and the arched height of hyoid bone were measured and compared the differences between male and female in order to deduce the analytic equation for gender estimation by hyoid bone., Results: Five connection conditions of hyoid bone were identified by the morphological characteristics, including complete ossification in both sides, no ossification in both sides, partial ossification in both sides, complete ossification in one side (partial ossification in the other side), and complete ossification in one side (no ossification in the other side). The values of the arched height of hyoid bone (x1) and the height of hyoid bone (x2) in male were both higher than that in female (P < 0.01). The analytic equation for gender estimation (y) was y = 0.438 x1 + 1.042x2-12.979. The discriminant value was -0.272 5 and the resolution was 88.2%., Conclusion: According to the gender characteristics of hyoid bone, the data of hyoid bone can provide helps for forensic practices.

Objective: To establish a CT staging method of sternal end of clavicle epiphyseal growth for teenagers by thin layer CT scan, multiplanar reconstruction (MPR) and volume reconstruction (VR) technologies., Methods: The CT imaging characteristics, which collected from 460 teenagers (aged from 15 to 25) in East and South China, were studied through both sternal ends of clavicles' imagings by thin layer coronal CT scan and axial CT scan, MPR and VR technologies. The parameters of sternal end of clavicle including the longest diameter of epiphysis, the longest diameter of metaphysis, their length ratio, area of epiphysis, area of metaphysis, and their area ratio were measured and calculated in order to establish new classification., Results: Based on the staging method of Schmeling, integrating the CT imaging reconstruction information of sternal end of clavicle, the new classification of sternal end of clavicle epiphyseal growth were divided into 1 to 5 stages, and stage 2 and stage 3, respectively, contained a, b and c sub-classification., Conclusion: This new classification has merits of large sample size, wide age range and easy operability. By using CT imaging reconstruction techniques and analyzing the data which closely related to sternal end of clavicle epiphyseal growth of teenagers, the feasibility of the staging method could be increased.

Objective: To study the status of EB virus (EBV) infection and the spectrum of EBV infection-related diseases., Methods: A total of 761 plasma samples with suspected EBV infection were collected from 761 children (aged from 22 days to 14 years) admitted between August 2010 and July 2011. EBV-DNA of 761 plasma samples was detected by real-time PCR. The epidemiological characteristics and final clinical diagnosis were analyzed based on the clinical data of these EBV-positive hospitalized patients., Results: A total of 109 cases with EBV infection were detected by real-time PCR, with a positive rate of 14.3%. There were significant differences in the positive rate of EBV-DNA among different age groups and between seasons (P<0.05). The positive rate of EBV-DNA in the baby group (<1 year old) was lowest (P<0.05), and the positive rate of EBV-DNA in summer was higher than in winter (P<0.05). The range of plasma EBV-DNA level in children with EBV-DNA positivity was 2.13 to 6.69 (median 3.72). Based on the final diagnosis of 62 EBV-positive hospitalized children, the most common disease was respiratory system infection (39%), such as acute bronchitis, acute upper respiratory infection and bronchopneumonia., Conclusions: The EBV-DNA positive rate is different among different age groups and between seasons. Respiratory system infection is a leading disease in hospitalized children who are EBV-DNA positive. Real-time PCR assay is useful for rapid and reliable clinical diagnosis of EBV in children.

Purpose: To investigate the prevalence of the Carabelli's trait in a Chinese population., Methods: Direct intraoral examination was conducted on 2758 Han Chinese people. The expression of Carabelli's trait was divided into 5 grades. Software SPSS 13.0 software package was used for statistical analysis. The trait frequency differences between two genders, between right and left sides were evaluated using Chi-square tests. Correlations in the presence of the trait between bilateral antimeric teeth and between first and second molars at the same side were determined by Kendall test., Results: The trait frequency was 36.18% (40.73% of the males and 32.13% of females) for the first and 1.39% (1.82% in males and 0.99% in females) for the second molars by individual counting method. Sex difference was statistically significant for the first molars (P<0.01). The bilateral concurrence rate was 72.97% and 48.57% for the first and second molar, and the bilateral correlation coefficient tau was 0.77 and 0.65, respectively. The correlation between the first and second molar in the same teeth district was weak (tau=0.13, P<0.01)., Conclusions: Evaluating the prevalence of Carabelli's trait in Chinese populations by using a standardized method has great anthropological significance.

The mating system of Caenorhabditis elegans includes selfing hermaphrodites and the males that cross with hermaphrodites. In lab-cultured C. elegans populations, the proportion of the males is very low, and till now, no detectable fitness of crossed offspring has been found. To explain why the C. elegans conserves a complicated pathway of male development through evolution, we compared the life history of the males and hermaphrodites under heat stress as well as the resistance of the populations with and without males against the heat stress. The results showed that though the life span of the males was more affected by heat stress, as compared with that of the hermaphrodites, the C. elegans populations with the males recovered faster after the stress, and the populations continuously subjected to the heat stress could maintain a higher proportion of the males than the populations cultured at normal temperature. These findings implied that under changeable natural environment, the existence of male C. elegans could be a significant evolutionary strategy for the population survival.

Objective: This study aimed to determine the prevalence rates of conventional risk factors and to analyze the features of coronary lesions in patients with coronary heart disease (CHD)., Methods: 3765 CHD cases were collected from the General Hospital of PLA in Beijing from 2009 to 2010 (2661 men, 1104 women). All the CHD patients enrolled in our study were diagnosed through angiography. Clinical and angiographic data of CHD patients were collected. After stratification on age and sex of the patients, logistic analysis method was used to evaluate the prevalence rates on conventional risk factors and to analyze the features of coronary lesions on CHD., Results: (1) More than two risk factors and advancing age of onset were commonly seen in female CHD patients. In those premature female CHD patients (age<45 years), there was a high proportion the habit of smoking. With the increase of age, proportions of patients with diabetes mellitus and hyperlipidemia also significantly increased. For male patients at different age, the proportions of smoking were high. (2) Data from logistic analysis suggested that diabetes mellitus could increase the prevalence of CHD on women (OR=2.05, 95%CI: 1.49-2.81, P<0.001), and smoking was a risk factor for men (OR=9.27, 95%CI: 7.68-11.19, P<0.001). (3) Along with the increase of age, female patients appeared to have more coronary vessel injures or severe coronary artery lesions. Different from the females, there was no change could seen on the characteristics of coronary lesions at different age for males., Conclusion: Prevalence rates on risk factors and the characteristics of coronary lesions appeared to be different with age. Diabetes mellitus and hyperlipidemia seemed to impact more on women while smoking seemed to impact more on men.

Objective: To explore the growth status of epiphysis of sternal end of clavicle using thin-section computed tomography (CT) and to study the relationship between the status and the chronological age of Sichuan Han nationality youth., Methods: According to the Schmeling's report and the age range of our samples, the ossification status of medial clavicle epiphysis was classified as four stages. CT films of 565 patients between 15 and 25 years were studied based on the classification and analyzed statistically., Results: There was no statistical difference between the sexes (P > 0.05). The calculated empiric distribution function showed that 100% of stage 1 patients were under 18 years, 75% of stage 2 patients were under 18 years, 94.5% of stage 3 patients were over 18 years, and 100% of the stage 4 patients were over 20 years, respectively., Conclusion: The ossification of medial epiphysis of the clavicle for those around 18 years has certain regular. These characteristics can be used for forensic identification of the skeletal age, especially 18 years, which is the criminal responsibility age.

When women face social and cultural inequality they inevitably bear increased HIV infection risks. The success of antiretroviral therapies in suppressing HIV's viral load and prolonging patient lives has made HIV a treatable chronic disease. Given the same follow-up treatments, research shows no significant differences between men and women in terms of either clinical, immunological or virological parameters at baseline or mortality after one year of antiretroviral therapy. Also, advances in assisted reproductive technologies now makes having HIV-free children possible for HIV couples. Gender equality and human right are essential to effective HIV prevention. Nurses must take all appropriate measures to eliminate discrimination against women in HIV prevention, treatment and care programs in order to ensure equal gender access to critical healthcare services.

Objective: To study the bone mineral development and the factors influencing the development in preterm infants., Methods: Ninety preterm and 90 term infants followed up by the child health care service were randomly enrolled. Tibia quantitative ultrasound measurements were used to evaluate bone mineral density described as supersonic speed of sound (SOS) and Z scores at 6 months old (corrected gestational age for preterm infants). The factors influencing bone mineral development were investigated by questionnaire., Results: The SOS values and Z scores in term infants were significantly higher than those in preterm infants at 6 months old. In the preterm group, the SOS values and Z scores were significantly different in infants with different birth weights or gestational ages (P<0.05). The SOS values in preterm infants with different weaning time were significantly different. The Z scores in female preterm infants were significantly higher than that in males (P<0.05). Multiple regression analysis indicated that weaning time and daily time of outdoor activities were independent factors influencing SOS values in preterm infants., Conclusions: It is helpful to promote bone mineral development by an appropriate weaning time or increasing the time of outdoor activities in preterm infants.

The traditional costicartilage analysis inspection is limited to morphological inspection. In recent years, with the development of forensic radiology and molecular genetics, the costicartilage analysis inspection technology has been further enriched and developed. At present, the costicartilage analysis inspection technology have been able to be used in the practice of forensic medicine. This paper reviews the research advances about the costicartilage analysis inspection technology in the identification of human gender, age and so on in order to provide the references for forensic appraisers.

Objective: To investigate the key parameters of three-dimensional anatomy of the proximal humerus and compare the differences between male and female, and between left and right sides in Chinese by volume rendering technique with multi-slice spiral CT (MSCT) so as to provide a reference for a new prosthesis of the proximal humerus which can adjust to the anatomical characteristics of Chinese., Methods: A total of 100 healthy volunteers were collected from Chongqing of China, including 59 males and 41 females with an average age of 40.4 years (range, 21-57 years). The humeral retroversion angle (RA), neck-shaft angle (NSA), medial offset (MO), and posterior offset (PO) were measured by volume rendering technique with MSCT. The average values were compared between male and female and between left and right sides, the correlation of these parameters was also analysed., Results: In 100 volunteers (200 sides), the RA was (19.9 +/- 10.6)degrees, the NSA was (134.7 +/- 3.8)degrees, the MO was (4.0 +/- 1.1) mm, and the PO was (2.6 +/- 1.3) mm. There were significant differences in RA and MO between left and right sides (P < 0.05); there was no significant difference in NSA and PO between left and right sides (P > 0.05). The PO and RA of both sides in male were significantly larger than those in female (P < 0.05); the NSA and MO in male were similar to those in female (P > 0.05). PO was correlated positively with RA (r = 0.617, P = 0.000); MO was not correlated with NSA (r = - 0.124, P = 0.081)., Conclusion: Because of significant side differences in RA and MO, and significant gender differences in RA and PO, the differences should be considered in the design of new proximal humeral prosthesis and proximal humerus reconstruction.