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The study was to explore the effect of Yucca schidigera extract on growth, odorous substances, microorganism and the gas produced in cat feces. Twenty-four healthy cats of similar weight and age were firstly selected, and these cats were pre-fed with the basic cat food without Yucca schidigera extract for 9 d, and then seven cats were selected for cat food containing Yucca schidigera extract experiment according to the results of fecal related tests, food was supplemented with 500 g/t Yucca schidigera extract and fed 100 g daily for 21 d. The results showed that there was no significant difference in the body weight of cats on the 1st and 21st day of the experiment (P>0.05), and there was no significant difference in the content of indole in cat feces on the 1st, 14th and 21st day of the experiment (P>0.05). On the 14th and 21th day of the experiment, the content of skatole in cats was significantly lower than that on the 1st d of the experiment (P<0.05). Compared with the first day, the proportions of carbon dioxide, methane and hydrogen sulfide production in cat feces after 48 h fermentation on the 21st day of the experiment were significantly decreased (P<0.05). There was no significant difference in the total number of colonies, E. coli and lactic acid bacteria in cat feces between 1 d and 21 d (P>0.05). The study indicates that Yucca schidigera extract in cat food has no significant effect on the growth indicators of cats, but can significantly decrease the skatole, arbon dioxide, methane and hydrogen sulfide in cat feces, which is helpful to improve the odor of cat feces. [ABSTRACT FROM AUTHOR]

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The purpose of this study was to isolate a skatole-degrading bacterium, and provide a high efficiency microorganism for the degradation of odor chemicals released from composting manure. A liquid culturing method was used to enrich skatole-degrading bacteria, and a pure culture was isolated using plate-streaking. The degrading ability of the candidate bacteria was pre-determined by smell assessment and chemotactic responses to skatole serving as the sole nutrition. The efficacy of strain Rp3 to degrade skatole at different concentrations was analyzed by high performance liquid chromatography（HPLC）, and was identified based on the morphological and physiological characteristics, in combination with 16S rRNA gene sequence analysis. A skatole-degrading bacterium, Rp3, was isolated from soil under sheep manure compost collected from Shanxi Province. This strain showed chemotactic response to skatole. HPLC analysis indicated that the degradation time extended with the increase of skatole concentration. Strain Rp3 could completely degrade skatole at a concentration of 50 mg·L-1 after co-culturing with skatole at 28 ℃ for 24 h, while the degrading time extended to 48 h with an efficacy of 98.4%, when the concentration was raised to 100 mg·L-1 . The optimum pH for Rp3 growth was pH 7~8, and Rp3 exhibited good tolerance to salt stress, demonstrated by normal growth in a solution of 0~10% NaCl. Strain Rp3 was identified as Rhodococcus pyridinivorans, based on its morphological characteristics, the results of Biolog identification system and 16S rRNA gene sequence analysis. Strain Rp3 was identified as Rhodococcus pyridinivorans, and was shown to be a highly effective strain in degrading skatole, an odor chemical in compost [ABSTRACT FROM AUTHOR]

A high performance liquid chromatographic (HPLC) method with fluorescence detection (FD) was established to simultaneously determine plasma indole and skatole. Plasma samples were pretreated by liquid-liquid extraction. Chromatographic separation was accomplished on a Shim-Pack VP-ODS column (150 mm×4. 6 mm, 4.6 μ m) using an isocratic mixture of 15 mmol/L sodium dihydrogen phosphate solution and methanol (40:60, v/v). The fluorescence excitation and emission wavelengths were 274 nm and 340 nm, respectively. The linear ranges were 2.22-88.89 μ g/L for indole and 1.11-44.44 μ g/L for skatole. The detection limits were 0.11 μ g/L and 0.06 μ g/L for indole and skatole, respectively. The recoveries were in the range of 95.5%-112.3% with the relative standard deviations less than 6.8%. The method was successfully applied to the analysis of plasma from healthy pregnant women ( n =46) and pregnant women with hepatitis B virus (HBV) infection ( n =29). The results showed that plasma indole and skatole levels were significantly different between two groups. In pregnant women with HBV infection, the concentrations of indolic compounds were positively associated with transaminase levels.