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Objective To analyze the relationship of miRNA-146a gene polymorphisms and ischemic post- stroke depression (PSD). Methods The SNP rs2910164 in the miRNA-146a gene in 200 ischemic PSD patients (case group) and 200 ischemic non-PSD patients (control group) was analyzed using the SNaPshot technique. The relative expression levels of miRNA-146a in the peripheral blood of two groups were determined. Results The case group showed significantly different allele and genotype frequencies of the SNP rs2910164 as compared with the control group (G vs. C : P = 0.003 ; GG + CG vs. CC : P = 0.012). Meanwhile, the case group presented a significantly lower level of miRNA-146a relative expression (P < 0.05). Within the control group, additionally, the subgroup of GG + CG genotypes demonstrated a significantly lower level of miRNA-146a relative expression compared with the subgroup of CC genotypes (P < 0.05). Conclusion SNP rs2910164 in the miRNA-146a is associated with the susceptibility to ischemic PSD, and G allele may play a role in the ischemic PSD. [ABSTRACT FROM AUTHOR]

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Aim To study the causal relationship between coffee consumption and atherosclerosis risk. Methods Based on the public genome database IEU Open GWAS project website, relevant data were obtained, and instrumental variables closely related to exposure and outcome were screened. Mendelian randomization analysis was performed using the inverse variance weighted method, weighted median method, and MR Egger method to assess the causal relationship between coffee consumption and arteriosclerosis (including coronary atherosclerosis, peripheral atherosclerosis, cerebral atherosclerosis, and other atherosclerosis). In addition, the Egger intercept method was used to detect the level of pleiotropy, the Cochran Q test was used to assess the heterogeneity, and the leave one out method was used to perform sensitivity analysis, so as to ensure the robustness of the results. Results The study showed that the F-values of the instrumental variables included in the study were all greater than 10, and there was no weak instrumental variables bias. Coffee consumption was positively correlated with the risk of coronary atherosclerosis (OR=1. 535 5, 95%CI=1. 108 4 ~ 2. 127 2, P =0. 009 9), peripheral atherosclerosis (OR=2. 098 6, 95%CI=1. 182 2 ~3. 725 7, P =0. 011 4), and other atherosclerosis (OR=1.864 7,95%CI=1.052 9 ~3.302 4, P =0. 032 6), but not with the risk of cerebral atherosclerosis. Heterogeneity test Q_pval>0. 05, level pleiotropy test interval P>0. 05. Conclusions The single nucleotide polymorphisms selected in the study are all strong instrumental variables, and there is no heterogeneity or horizontal pleiotropy, indicating reliable results. The research shows that coffee consumption increases the risk of coronary atherosclerosis, peripheral atherosclerosis and other atherosclerosis, and has no significant correlation with the risk of cerebral atherosclerosis. The health management of atherosclerosis patients should reduce coffee consumption. [ABSTRACT FROM AUTHOR]

Objective: To explore the genetic susceptibility of MIF and MMPs family gene polymorphisms to ankylosing spondylitis (AS), as well as the correlation of gene-gene, gene-environment interactions in the pathogenesis of AS, and to find high risk factors of AS and screening of high-risk groups of AS. Methods: A total of 180 AS patients and 345 controls were included in this casecontrol study. Selected the tag SNPs of MIF and MMPs family genes (MIF rs2070767, MIF rs1007888, MMP1 rs498186, MMP2 rs243866, MMP3 rs591058, MMP8 rs11225395), then DNA was extracted and genotyped, and the relationship between genotype, allele distribution frequency, genetic model and the incidence of AS and the risk factors were further analyzed. Generalized multi-factor dimensionality reduction method was used to analyze the correlation of the interaction between target genes and between genes and the environment and AS. The functional enrichment analysis and immune correlation analysis of MIF and MMPs were carried out. Results: The genotype distribution frequency of the smallest alleles of MIF rs1007888, MMP1 rs498186, MMP8 rs11225395 were higher risks in AS group than in control group (P<0.05). GG genotype of MIFrs1007888, GG genotype of MMP1 rs498186, CT/TT genotype of MMP3 rs591058, and TT genotype of MMP8 rs11225395 were related to the risk of AS. Gene-gene interaction analysis found that MMP3 rs591058 and MMP8 rs11225395 interaction models were the best models. Gene-environment interaction analysis found that MIF rs2070767, MIF rs1007888, MMP1 rs498186, MMP2 rs243866, MMP3 rs591058, MMP8 rs11225395, smoking, drinking, and obesity interaction models were the best models. MIF and MMPs related genes were enriched in matrix metalloproteinases, macrophage migration pathway and immune system regulation. Expression levels of MMPs were positively correlated with immune infiltration. Conclusion: The single nucleotide polymorphisms of MIF and MMPs are related to the susceptibility to AS, and there is an interaction between MIF and MMPs genes and the environment, which causes as through multiple biological processes, and may affect the process of as through immune infiltration. [ABSTRACT FROM AUTHOR]

【Objective】The study was conducted to clarify the genetic structure characteristics, genetic diversity level and molecular variation of different geographical populations of Conopomorpha sinensis Bradely, with an aim to provide scientific evidence for the study of its origin and evolution and provide reference information for the control of this pest in different regions.【Method】Using the C. sinensis from 5 production areas in southern China as materials, using GBS (Genotyping-by-sequencing) technology was used for samples genotyping, then SNP locus was identified with SAMTOOLS software, and phylogenetic tree was constructed according to SNP genotyping results. Finally, principal component analysis was performed.【Result】Sequencing of C. sinensis from 5 regions was conducted by GBS technology. A total of 27.39 Gb of highquality effective data were obtained, with an average of 5.48 Gb per sample. A total of 11 767 high-quality single nucleotide polymorphism (SNPs) loci were obtained. Phylogenetic tree analysis showed that the genetic relationship of Luzhou population was far from that of other populations. The results of principal component analysis showed that C. sinensis was divided into 2 groups in 5 regions, and the Luzhou population was significantly different from the other 4 populations. Both principal component analysis and phylogenetic tree analysis revealed that there were obvious differences in genetic level among C. sinensis from different geographical origins. The results of genetic diversity analysis showed that the observed heterozygosity was 0.2099-0.2531, the expected heterozygosity was 0.1937-0.2203, π was 0.2495-0.2748, indicating that the genetic diversity level of C. sinensis population was low. The results of AMOVA analysis showed that the genetic variation between populations (0.87%) was smaller than that within populations (99.13%), indicating that the variation mainly came from within populations rather than between populations.【Conclusion】The genetic diversity level of C. sinensis in different geographical populations was low, while the genetic differentiation degree of Luzhou population in Sichuan was high, and its genetic relationship was distant with other populations. [ABSTRACT FROM AUTHOR]

Aim To explore the association between insomnia and myocardial infarction (MI) using bidirectional two-sample Mendelian randomization (MR). Methods The summary statistics of MI were obtained from genome-wide association studies (GWAS), and single nucleotide polymorphisms (SNP) associated with insomnia phenotype were selected as instrumental variables. Inverse variance weighting (IVW) was used to evaluate the causal relationship between insomnia and MI. Results MR analysis showed that there was positive relationship between insomnia and MI (IVW: OR=1. 015, 95%CI 1. 004 ~1. 027, P =0. 007). There was no horizontal pleiotropy and heterogeneity in instrumental variables. Sensitivity analysis suggested that MR analysis results were stable. Reverse MR analysis showed no causal relationship between MI and increased risk of insomnia (IVW: OR=0. 725,95% CI 0. 466 ~ 1. 126, P = 0. 152), and the statistical results were stable after the removal of outliers. Conclusion Insomnia is a risk factor for MI and there was positive relationship between insomnia and MI. However, there was no causal relationship between MI and an increased risk of insomnia. [ABSTRACT FROM AUTHOR]

Objective To investigate the causal relationship of the intake of fiber-rich foods, grains, fruits and vegetables with frailty through two-sample Mendelian randomization analysis. Methods We obtained genome-wide association study (GWAS) data from the IEU database, including single nucleotide polymorphism (SNP) data from the European population without sample overlap. The selected instrumental variables satisfied three key assumptions, with grain, fruit, and vegetable intake considered as exposure factors and frailty as the outcome factor. The inverse variance weighted (IVW) method was used as the primary analysis method, complemented by MR-Egger, weighted median, simple mode, and weighted mode methods for result validation and accuracy assessment. Additionally, various sensitivity analysis methods were employed to detect heterogeneity and horizontal pleiotropy. Results A total of 31 SNPs for grain intake, 46 SNPs for fruit intake, and 13 SNPs for vegetable intake were extracted as instrumental variables for analysis. The IVW method indicated that grain intake (OR=0.756, 95%CI: 0.679-0.841, P<0.001) and fruit intake (OR=0.881, 95%CI: 0.777-0.999, P=0.048) were protective factors against the risk of frailty, while vegetable intake (OR=1.201, 95%CI: 0.960-1.501, P=0.109) showed no causal relationship with frailty risk. Sensitivity analyses confirmed the robustness of these results. Conclusion There is a negative causal relationship between grain and fruit intake and the risk of frailty, whereas vegetable intake does not have a causal relationship with frailty risk. [ABSTRACT FROM AUTHOR]

Objective To investigate whether there is a causal relationship between gut microbiota and autism in children using the Mendelian randomization (MR) analysis method. Methods Genetic data from 18 340 individuals collected by the MiBioGen consortium across 24 cohorts were utilized. Reliable instrumental variables were constructed by selecting genetic variations associated with 33 microbial taxa. Using results from a genome-wide association study (GWAS) on susceptibility genes for autism in children conducted in Europe, we comprehensively assessed the causal link between gut microbiota and autism using inverse variance weighting (IVW), MR-Egger regression, and weighted median estimation (WME). Results Among the three selected single nucleotide polymorphisms (SNPs), the abundance of Bacteroidetes was significantly associated with autism (OR=0.508, 95%CI: 0.267-0.966, P=0.039). The IVW results indicated that for every one standard deviation increase in Bacteroidetes abundance, the average risk of developing autism decreased by 49.2%. Conclusion This study suggests a negative causal effect of Bacteroidetes abundance on the risk of developing autism in children. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Contemporary Pediatrics is the property of Xiangya Medical Periodical Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Osteosarcoma, which primarily affects children and adolescents, is a highly malignant bone tumor with high rates of disability and mortality. Therefore, the exploration of biological markers related to its occurrence, development, and prognosis is crucial. Genome-wide association studies have revealed the vital role of genetic polymorphisms in the pathogenesis of osteosarcoma. This study aims to provide new insights into reliable biomarkers of osteosarcoma through the analysis of the functional single-nucleotide polymorphisms of tumor-related genes.

: [Objective) The study aimed to analyze the single nucleotide polymorphisms (SNPs) of LEP and LEPR genes and their correlation with body condition score (BCS) in Texel x Kazak hybrid (Teha) ewes. (Method) The missense mutations in exon 18 of LEP and LEPR were screened by PCR sequencing technology, and the SNPs were genotyped by KASP typing method, and the association between the genotyping results and their BCS was analyzed. (Result) (i) The results showed that one SNP site, rs1093355763, was found in the LEP gene, and three SNPs sites, rs428867159, rs412929474 and g. 2770 G > A, were found in the LEPR gene. All of them belong to exon missense mutations, rs428867159 and rs412929474 belonged to moderate polymorphism (0.25 A be-longed to low polymorphism (PIC≤0.25). All of them were in Hard-Weinberg equilibrium (P<0.05). (ii) In the genotype effect of rs1093355763, the body weight of CA genotype was significantly higher than that of CC genotype at the middle stage of lactation (P<0.05, the samw as below). Among the genotype effects of rs428867159, the BCS of TT genotype was significantly higher than that of CC genotype and CT genotype at lambing stage. In the genotype effect of g. 2770G > A locus, the BCS of GA genotype was significantly higher than that of GG genotype at 120 days of pregnancy, and the BCS of GG genotype was significantly higher than that of GA genotype at weaning stage. The body weight of GA genotype was significantly higher than that of GG genotype at 120 days of pregnancy. (Conclusion) The rs1093355763, rs428867159 and g. 2770 G > A loci in LEP and LEPR genes can be used as molecular markers for BCS and body weight of Teha ewes in sheep breeding. [ABSTRACT FROM AUTHOR]

Objective To investigate the causal relationship between body mass index (BMI) and lumbar disc herniation, to provide recommendations for clinical prevention and treatment. Methods Summary data from the Genome-wide Association Study (GWAS) in the UK Biobank of the IEU Open GW AS project (htt ps://gwas.mrcieu.ac.uk/) were used for analysis. Single nucleotide polymorphisms (SNPs) closely related to BMI were selected as instrumental variables, and lumbar disc herniation was the outcome variable. The sample data were extracted from European populations, with the BMI group consisting of 454 884 individuals and 9 851 867 SNPs, and the lumbar disc herniation group consisting of 462 933 individuals and 9 851 867 SNPs. The main analysis methods included inverse -variance weighted (IVW), MR -Egger regression, and weighted median estimator (WME), with the simple mode and weighted mode as auxiliary analyses. Mendelian randomization (MR) analysis was conducted to explore the causal relationship between BMI and lumbar disc herniation. Sensitivity analysis was performed using the leave -one -out method, and the MR -Egger regre ssion intercept test, CochranJ s Q test, funnel plot observation for heterogeneity, symmetry, and scatter plot observation for directional consistency were conducted. The odds ratio (OR) and 95% confidence interval (CI) were used to evaluate the causal relationship between BMI and lumbar disc herniation. Results A total of 419 SNPs associated with BMI (P<5xl0-8,产<0.001) were included as instiumental variables. The analysis using MR-Egger (O2-=1.002, 95%CI: 0.997-1.007), WME (0-=1,003, 95%CI: 1.000--1.006): IVW (O/-=1.004, 95%CI: 1.002-1.006), simple mode (OR=1.006: 95%CI: 0.998-1.014), and weighted mode (0-=1.004, 95%CI: 0.999-1.009) indicated that an increase in BMI was positively associated with lumbar disc herniation. The P values for WME and IVW were 0.030 and 335X10-6, respectively, with a significance level of a =0.05. The P values for MR-Egger, simple mode, and weighted mode were all >0.05, indicating no statistical significance. The consistent direction of effect among the five analysis methods suggested tliat an increase in BMI was associated with a higher risk of lumbar disc herniation. The intercept for MR- Egger regression was 4.2x10 巳 the standard error was 4.6x10 巴 and the P value was 0.364, indicating the absence of pleiotropy in the instrumental variables. A forest plot generated using the leave-one-out method showed no obvious bias, and the funnel plot exhibited overall symmetry. Conclusion There is a causal relationship between BMI and lumbar disc herniation, and an increase in BMI may be an important factor in triggering lumbar disc herniation. [ABSTRACT FROM AUTHOR]

Objective To explore the causal relationship between exercise, sedentary behavior, and the risk of sarcopenia, and to gain a deeper understanding of the pathogenesis of this disease, to provide guidance for prevention, treatment, and rehabilitation. Methods Mendelian randomization (MR) analysis was conducted using data from genome-wide association studies. Single nucleotide polymorphisms that were significantly associated with the exposure and with the outcome were selected, and multiple MR methods were employed, including inverse variance weighting, weighted median, MR-Egger, weighted mode, simple mode, and MR-PRESSO. Heterogeneity, robustness, and consistency of the results were assessed through CochranJ s Q statistic, MR -Egger regression, and leave -one -out sensitivity analysis. Results The inverse variance weighting method showed that low-intensity exercise had a positive causal relationship with the risk of sarcopenia (OR=1.76, 95%CI: 1.09-2.85, P=0.02; OR二0.3& 95%CIi 0.14-1.00, P<0.05; O2-=2.13, 95%CI: 1.75-2.5& P<0.001), while sedentary behavior had a negative causal relationship with the risk of sarcopenia (OR二0.74, 95%CI: 0.64-0.85, P<0,001; OR二 1.51, 95%CI: 1.24-1.83, P<0.001; OR=0.73, 95%CI: 0.70-0,77, P<0,001). Sensitivity analysis showed no evidence of reverse causality, pleiotropy, or heterogeneity. Conclusion The results of this study confirm a reverse causal relationship between low-intensity exercise and sarcopenia, while sedentary behavior has a positive causal relationship with sarcopenia, which provides genetic evidence to support the treatment of sarcopenia. [ABSTRACT FROM AUTHOR]

Objective To investigate the causal relationship between PM25 exposure and chronic kidney disease (CKD) as well as related indices including eGFR and urinary albumin using a two-sample Mendelian (MR) approach. Methods MR analysis was conducted using large-scale publicly available genome-wide association study (GWAS) summary data. The inverse variance weighted (IVW) method served as the primary approach, accompanied by MR-Egger, weighted median, simple model, and weighted model methods. MR-PRESSO, Cochran Q test, MR-Egger, and leave-one-out methods were employed to test the robustness of results. Results Neither IVW nor the four other methods found a causal between PM25 and CKD (Oi-二0.915, 95%CI: 0370 to 2.26& P=0.849), eGFR (OR=0.997: 95%CI: 0.988 to 1.006, P=0.542), or urinary albumin (02- =0.972, 95% CI: 0.164 to 5.760, P=0,975). Sensitivity analyses did not reveal outliers, pleiotropy, or the influence of single nucleotide polymorphisms (SNPs) on the overall effect estimate. Conclusion This study does not support a causal relationship between PM25 exposure and CKD or its related indices including eGFR and urinary albumin. [ABSTRACT FROM AUTHOR]

Osteosarcoma, which primarily affects children and adolescents, is a highly malignant bone tumor with high rates of disability and mortality. Therefore, the exploration of biological markers related to its occurrence, development, and prognosis is crucial. Genome-wide association studies have revealed the vital role of genetic polymorphisms in the pathogenesis of osteosarcoma. This study aims to provide new insights into reliable biomarkers of osteosarcoma through the analysis of the functional single-nucleotide polymorphisms of tumor-related genes. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Contemporary Pediatrics is the property of Xiangya Medical Periodical Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To study the expression levels of long non-coding RNA (lncRNA) H19 and insulin-like growth factor 2 (IGF2) genes in breast cancer tissue, and to analyze their imprinting status. Methods: Real-time fluorescence quantitative PCR (RT-qPCR) method was used to detect the expression levels of H19 and IGF2 mRNA in breast cancer tissue and adjacent tissue, and the differences in the expressions of H19 and IGF2 mRNA in breast cancer tissue and adjacent tissue were analyzed; single nucleotide polymorphism (SNP) was used to distinguish the allele expression status (homozygous or heterozygous). For heterozygous IGF2 (Apa Ⅰ site) or H19 (Alu Ⅰ site) in genomic DNA, imprinting analysis was used to detect the imprinting status of H19 and IGF2 in breast cancer tissue, that were maintenance of imprinting (MOI) or loss of imprinting (LOI); the relationship between the expressions of H19 and IGF2 and molecular subtypes in breast cancer tissue were also analyzed. Results: The RT-qPCR results showed that the expression levels of H19 and IGF2 mRNA in breast cancer tissue were higher than those in adjacent tissue (P<0. 01). There was a positive correlation between the expression levels of H19 mRNA and IGF2 mRNA (r=0. 567, P<0. 01). Compared with adjacent tissue, the expression levels of H19 mRNA in cancer tissue of the breast cancer patients with various molecular subtypes were increased (P<0. 05 or P<0. 01). LOI was observed in both H19 and IGF2 in breast cancer tissue, and the incidence of IGF2 LOI was 36. 7%, which was higher than that of H19 LOI(4. 3%). The RT-qPCR results showed that the expression level of IGF2 mRNA in breast cancer tissue in IGF2 LOI group was significantly higher than that in IGF2 MOI group (P<0. 01). Conclusion: The expression levels of H19 and IGF2 mRNA in breast cancer tissue are significantly higher than those in adjacent tissue. The incidence of IGF2 LOI is higher than that of H19 LOI, and IGF2 LOI may be one of the key factors in the pathogenesis of breast cancer. [ABSTRACT FROM AUTHOR]

Copyright of Acta Agriculturae Zhejiangensis is the property of Acta Agriculturae Zhejiangensis Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Acta Agriculturae Zhejiangensis is the property of Acta Agriculturae Zhejiangensis Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Forensic Science & Technology is the property of Institute of Forensic Science, Ministry of Public Security and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Sichuan University (Medical Science Edition) is the property of Editorial Board of Journal of Sichuan University (Medical Sciences) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To evaluate the potential causal relationship between blood metabolites and the risk of heart failure by two-sample Mendelian randomization. Methods Based on a genome-wide association study containing 486 blood metabolites, single nucleotide polymorphisms (SNPs) independently associated with blood metabolites was selected as instrumental variables. The heart failure data were derived from the Genome-Wide Association Study of the Molecular Epidemiology Research Consortium for Therapeutic Targets in Heart Failure. The potential causal relationship between them was analyzed by inverse variance weighting method, MR-Egger regression method, and weighted median method, and the sensitivity analysis was performed using MR-Egger regression intercept, Cochran Q test, MR-PRESSO, and leave-one-out method. Results In total 7 516 SNP associated with blood metabolites were selected as instrumental variables. The results of inverse variance weighting analysis showed that pantothenic acid was associated with heart failure (OR=1.29, 95% CI: 1.03-1.63). Sensitivity analysis results indicated that the results were robust. Conclusion This study suggests that there is a positive causal relationship between pantothenic acid and the risk of heart failure, which may provide new insights into the pathogenesis and drug targets of heart failure. [ABSTRACT FROM AUTHOR]

Objective To evaluate the causal relationship between tea drinking and cholelithiasis by two-way Mendelian randomization. Methods The instrumental variables for tea drinking were determined from 447 485 participants in the UK Biobank genome-wide association study (GWAS). A total of 32 single nucleotide polymorphisms (SNPs) associated with tea drinking were used for two-sample Mendelian randomized analysis to evaluate the causal relationship between tea drinking and cholelithiasis. Cholelithiasis data were obtained from GWAS data of 35 712 cholelithiasis patients and 273 442 controls publicly available from the Finnegan Alliance. In this study, inverse variance weighted (IVW) was used as the main method to estimate causality, and sensitivity analysis was carried out to ensure the robustness of the results. Finally, reverse Mendelian randomization analysis was used to verify whether there was a reverse correlation. Results There was a causal re-lationship between tea drinking and cholelithiasis. In the IVW method, each additional standard deviation (2.85 cups/day) of tea consumption reduced the risk of cholelithiasis by 26.1% (OR =0.739, 95% CI: 0.536-0.990). Similar results were obtained in weighted mode (OR=0.652, 95% CI: 0.458-0.928), but there was no evidence to prove reverse correlation (IVW: P > 0.05). Conclusion The genetic evidence provided by two-way Mendelian randomized analysis shows that the increase of tea drinking reduces the risk of cholelithiasis, but there is no evidence of reverse association. [ABSTRACT FROM AUTHOR]

In order to identify effective genetic markers for transporter-associated with antigen processing 1 (TAP1), α (1, 2) fucosyltransferase 1 (FUT1), natural resistance-associated macrophage protein 1 (NRAMP1), mucin 4 (MUC4) and mucin 13 (MUC13) genes in Shanghai local pig breeds, including Shanghai White pig, Fengjing pig and Shawutou pig, and provide a reference for the characterization of Shanghai local pig breeds, polymerase chain reaction restriction fragment length polymorphism (PCR-RFLR) and sequence sequencing were applied to analyze the polymorphisms of the above genes, combined with some immune-related factors to explore the effects on immunity of the 3 Shanghai local pig breeds. The results showed that TAP1 and MUC4 genes had anti-diarrheal genotype GG in all 3 pig breeds, while anti-diarrheal genotype GG of MUC13 gene was detected only in Fengjing pig and Shawutou pig, and anti-diarrheal genotype AA of FUT1 and NRAMP1 genes were not detected in any of the 3 pig breeds. TAP1 gene was in moderate polymorphism in Shanghai White pig, Fengjing pig and Shawutou pig, the MUC4 gene of Shanghai White pig was in low polymorphism, the MUC4 gene of Fengjing pig and Shawutou pig was in moderate polymorphism, and the MUC13 gene of Shanghai White pig was in moderate polymorphism, among which the TAP1 gene of Shanghai White pig and Shawutou pig did not satisfy Hardy-Weinberg equilibrium, and the MUC4 gene of Fengjing pig did not satisfy Hardy-Weinberg equilibrium. The interleukin 12 (IL-12) level of MUC13 gene type AA was significantly higher than that of type AG in Shanghai White pig, the tumor necrosis factor α (TNF-α) index of TAP1 gene type AA was significantly higher than that of type GG and AG in Fengjing pig, and the IL-12 index of TAP1 gene type AG was significantly higher than that of type GG in Shawutou pig. Above results had some guiding significance for anti-diarrheal breeding and molecular selection of Shanghai White pig, Fengjing pig and Shawutou pig, and also laid the foundation for future anti-diarrheal breeding work of each local pig breeds in Shanghai. [ABSTRACT FROM AUTHOR]

Lung cancer is one of the malignant solid tumor types with the highest morbidity and mortality globally. In recent years, the survival of lung cancer has improved, but its prognosis and five-year survival are still poor. Risk factors of lung cancer include smoking, environmental pollution, occupational factors and chronic obstructive pulmonary diseases. Nicotinic acetylcholine receptors (nAChRs) have various forms and are widely involved in physiological, pathological and pharmacological processes of the body. Nicotine, one of the main components in tobacco, can be mediated by nAChRs after entering the human body. It is found that different subtypes of nAChRs have single nucleotide polymorphisms (SNPs), which can affect their functions. Genome-wide association study (GWAS), as an epidemiological research strategy, can better mine SNPs sites that are closely related to cancer occurrence, and then make it possible to find targets and drugs closely related to cancer occurrence and development. Based on the relevant research results at home and abroad, this paper systematically expounds the global prevalence of lung cancer and the relationship between nAChRs related gene SNPs and their function, and lung cancer, in order to provide a reference basis for accurate prevention and treatment of lung cancer.

To establish a genotyping technique for single nucleotide polymorphism(SNP) in highly homologous segments, the homology of 200 and 400 bp segments of SNP was evaluated by running local Blast, and the highly homologous segments SNP were screened out after evaluation. The first round of multiple long-range polymerase chain reaction (PCR) was used to capture long fragments of 9 highly homologous segments of SNP in 329 samples. The purified products of the first round of PCR were used as templates for amplicon library construction and sequencing. The results show that 2 928 SNP loci are detected in 329 samples, and the success rate of sequencing is as high as 98.885 6% . According to the Hardy-Weinberg(HWE) principle, the gene frequencies of SNP loci in 9 high homologous segments were calculated(p values were greater than 0.05). Compared with the gene frequencies obtained from thousands of genomes database in national center for biotechnology information (NCBI), it was found that the single base gene frequencies of the two segments were consistent(deviance<0.15). It is shown that the use of multi-long PCR targeted capture technology combined with second-generation sequencing, provided an accurate, rapid and large sample detection solution for highly homologous segments [ABSTRACT FROM AUTHOR]

[Objective] This study explored the relationship between GHR gene polymorphism and growth traits of Qinghai plateau yaks. [Method] The GHR gene was amplified by PCR in a test population f 440 healthy yaks at the ages of 30-36 months under same grazing conditions, before being sequenced and analyzed for SNPs using DNASTAR 7.1 software. The association between different genotypes and heir combined genotypes with growth and development indicators (body mass, body height, body slope ength, chest circumference and tube circumference) was then analyzed. [Result] Three SNPs of g. 732091C>G, g. 732195A>G and g. 732373G>A existed on the GHR gene of Qinghai plateau yaks, where two genotypes (AA and AG ) presented on g. 732195A>G and three genotypes presented on g. 732091OGCCC, CG and GG) and g. 732373G > A (GA, AA and GG). The χ² test showed that g. 732091C>G, g. 732195A>G and g. 732373G>A were in Hardy-Weinberg extreme imbalance, g. 732195A>G was low polymorphic (PIC<0.25), g. 732091OG and g. 732373G>A were moderately polymorphic (0.25G, g. 732195A>G and g. 732373G>A could significantly or highly significantly affect body weight and chest circumference in lateau yaks, and the dominant genotype was GG for g. 732091 OG and g. 732373G> A and A A for g. 732195A>G. The combined genotype GG-AA-GG individuals showed the best growth and development, specially in body slope length. [Conclusion] There were three SNPs in the GHR gene of Qinghai plateau yaks. Because of the association with some growth traits, it could be used as candidate genes for molecular marker-assisted selection in yaks. [ABSTRACT FROM AUTHOR]

Objective To establish a set of single nucleotide polymorphisms (SNP) detection protocol for inbred rats based on multiplex PCR-ligase detection reaction (LDR).Methods A total of 40 rats SNP sites were selected on chromosomes 1-20 and X of rats among 5 inbred strains of rats, and the 40 SNP sites were randomly divided into four groups. A genetic detection protocol for 4 groups of SNP in inbred rats based on multiplex PCR-LDR technology was constructed. 9 commonly used rat strains from two other domestic rat suppliers were detected by this protocol. Finally, the feasibility of this protocol was verified by comparing the amplification effects of different DNA polymerases by a third-party laboratory.Results When using the constructed SNP detection protocol for inbred rats to test 5 rat strains, all sites in each sample obtained good amplification results. The 9 commonly used rat strains from two other rat suppliers in china were also well amplified by this SNP detection protocol, and 40 SNPs were homozygous in each Inbred strain. The results of detection of the same rat DNA samples with three different DNA polymerases showed that the Multiplex PCR Kit, AmpliTaq Gold 360 DNA polymerase and Platinum II Taq hot start DNA polymerase had electrophoretic peaks of amplification products at all SNP sites in groups 1 to 3, and Platinum II Taq hot start DNA polymerase had one less electrophoretic peak of the amplification products at the SNP sites in group 4. In addition, inter-laboratory comparisons showed consistent results for the same amplification system.Conclusion Based on multiplex PCR-LDR technology, this study successfully established a SNP detection protocol for rats covering all autosomes and X chromosomes with the excellent stability and repeatability.

Objective This article explores the relationship between congenital tooth agenesis and related gene mutations, providing a reference for early diagnosis of the disease. Methods Clinical and radiographic examinations of a rare case of congenital tooth agenesis were conducted to evaluate the abnormal morphology and quantity of the teeth, as well as the overall health of the patient. Bidirectional sequencing of the PAX9 and MSX1 genes and whole-exome sequencing were conducted to identify potential genetic abnormalities. Sanger sequencing of the newly discovered mutation site was performed on the proband's son. Subsequently, the impacts of the mutations were evaluated through computational tools and a cell-based gene transfection assay. Results This is a rare case of tooth agenesis characterized by a congenitally missing first molar, a second molar with one single root and a supernumerary second premolar in the right mandibular dentition. The c.717 C>C/T in PAX9 is synonymous. The c.119C>G in MSX1 is a missense mutation predicted to be “benign” by Polyphen. Through whole-exome sequencing, we found a novel mutation, c.637-7 C>A in intron 3 of the WNT6 gene, which is predicted by MAXENT to influence the splicing of mRNA. Both the proband and his son carry this mutation. A cell-based gene transfection assay demonstrated that it did not alter the mRNA splicing of WNT6. Conclusion The interaction between single nucleotide polymorphisms may contribute to congenital tooth agenesis.

Objective To analyze the etiology of 22q13 deletion syndrome. Methods Genetic analysis was performed for a baby with 22q13 deletion syndrome using G-banded chromosome karyotype analysis and single nucleotide polymorphisms array (SNP-array) techniques. Chromosomal karyotype analysis of peripheral blood of parents was also conducted to identify the origin of chromosomal abnormality. Literature review was performed using the keywords of “t (13； 22) ” in PubMed, CNKI, Wanfang Data, Chongqing VIP databases. Cases of parental genetic t (13； 22) unbalanced translocation were analyzed. Results The mother’s karyotype was 46, XX, while the father’s karyotype was 46, XY, t (13； 22) (q31； q13.3) . The results of SNP-array analysis showed 35.80 Mb fragment duplication in 13q31.1q34 and 1.10 Mb fragment deletion in 22q13.3 in the premature baby. The karyotype of the premature baby was 46, XY, der (22) t (13； 22) (q31； q13.3) pat. According to literature review, three patients presented with mental retardation, epilepsy, facial deformity and atrial septal defect, etc. One of them died 11 d after birth. Conclusions The child carries a paternally inherited der (22) t (13； 22) (q31； q13.3) translocation. Specifically, 35.80 Mb fragment duplication is observed in 13q31.1q34, which is abnormal and pathogenic. 1.10 Mb fragment deletion is noted in 22q13.3, which includes 22q13 deletion syndrome (PMS) . [ABSTRACT FROM AUTHOR]

【Objective】This study aims to investigate SNP markers associated with leaf-type related traits in order to provide a reference for molecular marker-assisted breeding of tea plants. 【Method】In this study, simplified genome sequencing (GBS) was conducted on 91 natural tea populations collected in Sichuan. A genome-wide association analysis (GWAS) based on the GLM (Q) model was performed using 863, 468 SNP markers obtained from sequencing. Eight leaf-type related traits, including leaf length (YC), leaf width (YK), leaf length-width ratio (CKB), leaf area (YS), leaf color (YSZ), leaf tip (LD), leaf vein logarithm (YM), and leaf margin (YY), were investigated.【Result】A total of 38 significant SNP markers were detected at a p-value of ＜0.01, and there were 1 SNP marker associated with YC, 24 SNP markers associated with CKB, 6 SNP markers associated with YSZ, 2 SNP markers associated with YD, 1 SNP marker associated with YM and 4 markers associated with YY. The 38 significant SNP markers could explaine the association traits between 31.37% and 60.70%. Further candidate gene mining within the 50 kbp region upstream and downstream of the significant markers yielded. Additionally, 8 candidate genes were further identified, CSA011953 (Sec11a), CSA027293 (CAD3), CSA020561 (GAE2), CSA008530 (AIM1), CSA008531 (MFP), CSA008532 (AIM1), CSA008763 (CYP72A154), and CSA029592 (RABG3F). Through Go and Ko annotation, CSA027293 (CAD3) and CSA008763 (CYP72A154) could be closely associated with leaf length-width ratio (CKB) and leaf color (YSZ). 【Conclusion】The 38 identification of SNP markers and two candidate genes associated with tea leaf shape traits provide valuable genetic resources and a reference basis for the precise localization of genes regulating tea tree leaf shape and for molecular marker-assisted breeding. [ABSTRACT FROM AUTHOR]

【Objective】By analyzing the genetic diversity of 20 strains of Pleurotus eryngii and the characters of mycelium and fruiting bodies, it provides a certain basis for the protection and utilization of new molecular markers in P. eryngii resources, and also provides a theoretical basis for the accurate location of major trait genes, cross breeding, and phenotype prediction.【Method】The genome of 20 strains of P. eryngii collected in different regions of China was resequenced, the SNP loci obtained from the analysis were used as markers for genetic diversity analysis, and the growth rate of their mycelium on PDA dishes, yield, fruiting body length, cap diameter, stipe length, stipe diameter, fruiting body hardness, fruiting body fracturability, fruiting body chewiness and fruiting body gumminess were measured, the principal component analysis was carried out for 10 traits, and the results of each trait and genetic diversity were analyzed together to evaluate the correlation between genetic diversity and traits.【Result】When the genetic distance was greater than 0.08, 20 strains of P. eryngii can be divided into 6 groups. The characters of strains in each group were different from those in other groups, and the characters of strains in the group were relatively close. Group V contains 13 strains, and the characters were the most prominent; Followed by group II contains 3 strains, the rest of the several groups contained only 1 strain, there were differences in the traits between different groups, and there were very small differences within a group. 【Conclusion】The strains of P. eryngii from different regions in China have certain differences in genetic diversity, mycelium growth rate, fruiting body characters and texture, among which the strains of Pleurotus eryngii with excellent and balanced characters are clustered in group V, indicating that there is a strong correlation between SNP markers and fruiting body characters in Pleurotus eryngii. In breeding practice, SNP markers can be used to predict the characters of strains, and strains with long genetic distance and outstanding individual characters can be used as parents for cross breeding, which can greatly reduce breeding cycle and improve breeding efficiency and precision. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To establish a set of single nucleotide polymorphisms (SNP) detection protocol for inbred rats based on multiplex PCR-ligase detection reaction (LDR). Methods A total of 40 rats SNP sites were selected on chromosomes 1-20 and X of rats among 5 inbred strains of rats, and the 40 SNP sites were randomly divided into four groups. A genetic detection protocol for 4 groups of SNP in inbred rats based on multiplex PCR-LDR technology was constructed. 9 commonly used rat strains from two other domestic rat suppliers were detected by this protocol. Finally, the feasibility of this protocol was verified by comparing the amplification effects of different DNA polymerases by a third-party laboratory. Results When using the constructed SNP detection protocol for inbred rats to test 5 rat strains, all sites in each sample obtained good amplification results. The 9 commonly used rat strains from two other rat suppliers in china were also well amplified by this SNP detection protocol, and 40 SNPs were homozygous in each Inbred strain. The results of detection of the same rat DNA samples with three different DNA polymerases showed that the Multiplex PCR Kit, AmpliTaq Gold 360 DNA polymerase and Platinum II Taq hot start DNA polymerase had electrophoretic peaks of amplification products at all SNP sites in groups 1 to 3, and Platinum II Taq hot start DNA polymerase had one less electrophoretic peak of the amplification products at the SNP sites in group 4. In addition, inter-laboratory comparisons showed consistent results for the same amplification system. Conclusion Based on multiplex PCR-LDR technology, this study successfully established a SNP detection protocol for rats covering all autosomes and X chromosomes with the excellent stability and repeatability. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Contemporary Pediatrics is the property of Xiangya Medical Periodical Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To explore the causal relationship between gout and colorectal cancer using bidirectional two-sample Mendelian randomization (MR) analysis. Methods The data of gout 仇=462 933) and colorectal cancer (n=377 673) were extracted from the published genome -wide association studies (GWAS). By setting the association intensity threshold and eliminating linkage disequilibrium, the single nucleotide polymorphisms (SNPs) were screened out as instrumental variables (IVs), Inverse variance weighting (IVW) was used as the main analysis method, and weighted median, MR-Egger, simple model, and weighted model as supplementary analysis methods to evaluate causal effects. The heterogeneity was analyzed by Cochran Q test of IVW and MR-Egger. MR-Egger intercept and MR-PRESSO analysis were used to test the pleiotropy. Leave-one-out analysis was conducted to evaluate tlie stability of results. Results The IVW result of MR analysis of gout and colorectal cancer was (OR=0.948, 95%CI: 0.899-0.999), indicating a causal relationship between gout and reduced risk of colorectal cancer. No association (IVW, OR=0.996, 95%CI: 0.906-1.094) was found in the reverse MR analysis. The results of positive analysis suggested that there was some heterogeneity, but we adopted random effects to analyze, so we minimized the impact of heterogeneity. The results of leave -one method and pleiotropy test showed stability. Conclusion Our study confirms that gout reduces the risk of colorectal cancer, and there is no association in reverse direction, which needs to be verified by more clinical studies and animal trials as well as a larger sample database. [ABSTRACT FROM AUTHOR]

Objective To explore the causal relationship between body mass index (BMI) and anxiety disorder based on bidirectional Mendelian randomization using single nucleotide polymorphism (SNP) as instrumental variable (IV). Methods BMI was used as the exposure, and anxiety the outcome variable. The data were collected from the genome-wide association study (GWAS) data set. The GWAS data of BMI included 694 649 European descendants, and the anxiety GWAS data included 407 288 European descendants. A total of 524 SNPs highly related to BMI were selected as I Vs, with P < 5 X 10書 the linkage disequilibrium coefficient set to 0.001, and the width of the linkage disequilibrium region of 10 000 kb. Inverse variance weighting method (IVW), weighted median method (WME), MR-Egger regression, and weighted model were used for two sample Mendelian randomization analysis. Heterogeneity test, pleiotropy test, and leave-one-out test were performed to analyze the sensitivity. OR and 9 5% CI were used to demonstrate the causal relationship between BMI and anxiety disorder. Finally, reverse Mendelian randomization analysis was used to verify the robustness of the results. Results The causal effects based on the four methods showed the same direction. The results all suggested that there was a positive causal correlation between BMI and anxiety, but there was no reverse causal correlation. The OR (95 % CT) was 1.008 (1.003-1.013), P=0.003, for IVW method, and 1.008 (0.994-1.021), 1.008 (1.000-1.014), and 1.008 (0.996-1.019) for MR-Egger regression, WME, and weighted model, respectively. The results of heterogeneity test were >0,05, so we paid attention to the results of random effect [VW, The intercept of MR-Egger regression was close to 0 (Egger-intercept=3.53081 X 10-6, P=0.97), which indicated no pleiotropy for the selected SNPs. Leave-one-out test verified the robustness of MR analysis results. Conclusion BMI can increase the risk of anxiety disorder. [ABSTRACT FROM AUTHOR]

Meiotic recombination affects genome evolution in several manners such as gene conversion and base substitutions. The neighboring bases have a strong impact on the mutational bias, but it is unclear how the u neighbor-dependent effect" varies with recombination rate. In this study, we proposed a method of quantifying the dependence of mutations on immediately adjacent bases on the basis of conditional mutual information (CMI), and investigated how recombination rate affects the neighbor-dependent mutational bias using the human SNP data derived from the1000 Genomes Project. The results showed that at the genome-wide level， the dependence of mutations at SNP loci on immediately adjacent bases (i. e. average conditional mutual information) increased with recombination rate. Specifically, mutational bias increased with recombination rate when SNPs were flanked by A/G, C/G or C/T bases, but recombination rate inhibited mutational bias when SNPs were flanked by A/A or T/T bases. In addition, the higher the recombination rate is, the stronger the mutational bias in exons and intergenic regions. But the mutational bias in introns is inhibited by high recombination rates. [ABSTRACT FROM AUTHOR]

Chlorophyll is an essential pigment for plant photosynthesis. Drought tolerance affects chlorophyll synthesis, reduces photosynthetic efficiency, and decreases soybean yield. In order to explore the genetic loci of chlorophyll content in soybean under drought stress, drought resistance index (DRI) were calculated by chlorophyll contents under drought and well-watered conditions. Moreover, based on the DRI and re-sequenced genotypes of the soybean natural population (199 accessions) at 20x sequencing depth, the genome-wide association study (GWAS) was performed. The results showed that 237 single nucleotide polymorphisms (SNPs) in 18 loci on the chromosomes 1, 2, 3, 7, 10, 16, 17 and 18 were identified to associate with DRI. Moreover, 181 SNPs (76.37% of the total) were located on chromosome 16, of which 9 SNPs were located in the gene introns, 17 in exons, 21 in upstream or downstream, and 1 in 3' UTR. Based on these, 57 candidate genes flanking the associated SNPs were found, and according to the gene annotation, SNP mutation, and RNA expression, 3 candidate genes, Glyma.16G063600 (F-box protein), Glyma. 10G007000 (AP2-EREBP), and Glyma. 17G143900 (AP2/ERF) were screened out which might be responsible for the drought tolerance in soybean. Above results provided important theoretical foundation for drought tolerance genetic improvement in soybean. [ABSTRACT FROM AUTHOR]

The genetic diversity of species is a key factor in their adaptability and survival capability. Habitat fragmentation is recognized as one of the key factors causing biodiversity loss, and it has significant impacts on the genetic diversity of plant populations. Hydrocera triflora is an endangered species. Its genetic diversity is unclear and endangered mechanism has not been reported. In this study, 34 samples of H. triflora from seven populations were used to obtain single-nucleotide polymorphisms (SNP) by Restriction-site Associated DNA sequencing (RAD-seq). We discussed the endangered mechanisms of H. triflora by analyzing population genetic diversity and structure. Additionally, we combined these results with historical population dynamics analysis and predictions of potential species distribution in different climate scenarios. The results were as follows: (1) H. triflora had low genetic diversity (Ho=0.156 9, He=0.165 4, π=0.186 5) and high genetic differentiation coefficient; AMOVA analysis showed that genetic variation mainly occurred within populations. (2) The Mantel test indicated that there was a significant positive correlation between environmental distance and genetic distance, as well as geographical distance, with respective P-values of 0.041 2 and 0.008 2. (3) The effective population size of H. triflora had been continuously declining since the mid-Holocene. (4) The total potential distribution area of H. triflora change slightly in the future compared to that in the modern climate. However, in scenarios of high CO2 emissions, the high suitable area decreased significantly and changed into low suitable area, especially in the Malay Islands where the suitable habitat was almost to extinction. The results indicate that habitat fragmentation caused a sustained decrease in the genetic diversity and effective population size of H. triflora. Therefore, the low self-renewal capacity, as well as detrimental environmental conditions such as human disturbance and urbanization, are the primary factors contributing to its endangered state. It is recommended to strengthen the in-situ protection of H. triflora, employing techniques such as artificial pollination to enhance gene flow among populations and thereby increase genetic diversity, and at the same time, we should focus on protecting wetlands from destruction. [ABSTRACT FROM AUTHOR]

Sugars are always transported to the other organs via sugar transporters to play their important functions in plants. SWEET (sugars will eventually be exported transporters) is a kind of sugar transporters which plays important function in plant growth and development as well as in resistance or tolerance to diverse biotic and abiotic stresses. To analyze the expressions of SWEET genes during pod and seed developments and under different stress conditions in soybean, the SWEET genes were firstly analyzed based on the newly published cultivated and wild soybean genomes in the present study. Subsequently, the gene expressions were evaluated during pod and seed developments, as well as in resistance to soybean mosaic virus (SMV) and tolerance to low phosphorus. The results showed that 48 SWEET genes were identified in the cultivated soybean genome (Wm82a4v1), which encoded protein with length 174~354 amino acids and located on 15 chromosomes. Meanwhile, 51 SWEET genes were identified in the wild soybean genome (W05), which encoded length of 84~392 amino acids and located on 16 chromosomes. The phylogenetic tree of SWEET genes in cultivated and wild soybeans showed that they were clustered into 3 sub-groups. Moreover, 16 SWEET genes were expressed in soybean pods with the expressions of Glyma. 06G122200, Glyma. 14G159900 and Glyma. 14G160100 etc. increased during the pod development process. 12 SWEET genes were expressed in soybean seeds, and the expressions of Glyma.08G025100, Glyma.13G041300 and Glyma.14G120300 were increased during the seed development, suggesting their important roles for soybean seed development. The SWEET genes showed different expressions after the inoculation of SMV, and the expressions of Glyma.08G009900 and Glyma.13G264400 were induced in leaves of resistant variety, while had no change in the sensitive variety, implying that they might involve in SMV resistance. The expressions of Glyma.04G198400, Glyma.14G160100 and Glyma.15G211800 were induced in soybean roots after low phosphorus treatment, indicating their important functions in the tolerance to low phosphorus stress. In addition, the single nucleotide polymorphism(SNP)analysis of SWEET genes showed that 43 genes contained 220 nonsynonymous SNPs with 103 located in the conserved domains of encoding proteins. Thus, above results provided candidate genes for molecular breeding of soybean pod and seed yield and diverse stress resistances. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Contemporary Pediatrics is the property of Xiangya Medical Periodical Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

【Objective】The Paper aims to explor the relationship between single nucleotide polymorphisms (SNP) of 5'-flanking and Intron 1 in growth hormone (GH) gene and the comb traits of Ningdu yellow chicken, and find molecular markers for the comb traits selection of local chicken breeds.【Method】The comb trait data at seven different week age of 499 Ningdu yellow chickens were recorded including comb height, comb length, comb thickness, wattle length, wattle thickness and number of comb teeth. These traits were associated with SNPs in the 5'- flanking and Intron 1 regions of GH gene using statistical analysis system (SAS). 【Result】A total of 13 SNPs were significantly associated with comb height trait (P < 0.05), including C1272225T, G1272611A, C1271211T, C1270902T, A1270769G, G1270640C, T1270578C, G1270503C, T1270451C, C1270032T. A1270769G was significantly correlated with the comb height traits at seven different week age. G1272611A was significantly associated with the comb height traits at five different week age. A total of 10 SNPs were significantly associated with comb length trait (P < 0.05), including C1272225T, G1272611A, C1271211T, C1270902T, A1270769G, G1270640C, T1270578C, G1270503C, T1270451C, C1270032T. C1271211T was significantly correlated with the comb length at six different week age. G1272611A was significantly correlated with the comb length at five different week age. The locus G1272611A was significantly associated with both comb length and height trait at five consecutive weeks, which had two genotypes in this population, GG and GA, comb height or length of GG genotype individuals were significantly higher than those of GA genotype.【Conclusion】G1272611A could be used as a candidate molecular marker for selection of Ningdu yellow chicken comb traits. [ABSTRACT FROM AUTHOR]

Objective: To investigate the relationship between pregnancy-associated protein A (PAPP-A) and peroxisome proliferator-activated receptor-γ (PPAR-γ) gene polymorphisms and preeclampsia (PE) susceptibility and pregnancy outcomes. Methods: 125 PE patients (PE group) who were admitted to Shijiazhuang Maternal and Child Health Hospital from July 2018 to June 2021 and 125 healthy pregnant women (control group) who were were examined during the same period were selected. The clinical outcomes in the two groups were statistically analyzed and compared. According to the different pregnancy outcomes of PE patients, they were divided into poor group and good group. The single nucleotide polymorphism (SNP) sites of PAPP-A and PPAR-γ genes in peripheral blood deoxyribonucleic acid (DNA) samples of all subjects were detected, and the SNP genotype and allele frequency in the PE group and control group, good group and poor group were compared, and their relationship with PE and adverse pregnancy outcomes of PE were analyzed. Results: The genotype distribution of PPAR-γgene rs10865710 and rs4684847 locus and PAPP-A gene rs7020782 locus were significantly different between the PE group and control group. The frequencies of PPAR- γ gene rs10865710 allele G, rs4684847 allele T and PAPP-A gene rs7020782 allele C in the PE group were higher than those in the control group (P<0.05). Binary Logistic regression analysis showed that PPAR-γ gene rs10865710 GG genotype (OR=2.641) and G allele (OR=1.641), PPAR-γ gene rs4684847 CT genotype (OR=3.084) and T allele (OR=2.985), PAPP-A gene rs7020782 locus CC genotype (OR=2.104) and C allele (OR=1.875) were risk factors for PE occurrence (P<0.05). The incidence of total adverse pregnancy outcomes in the PE group was 33.60%, which was significantly higher than 5.60% in the control group (P<0.05). The genotype distribution of rs10865710 and rs4684847 locus of PPAR-γ gene and rs7020782 of PAPP-A gene locus in the poor group and good group were statistically different, the frequency of PPAR- γ gene rs10865710 allele G, rs4684847 allele T and PAPP-A gene rs7020782 allele C in the poor group were higher than those in the good group (P<0.05). Binary Logistic regression analysis showed that PPAR-γ gene rs10865710 GG genotype (OR=2.446) and G allele (OR=1. 503), PPAR-γ gene rs4684847 CT genotype (OR=2.225) and T allele (OR=2.013), PAPP-A gene rs7020782 locus CC genotype (OR=2. 005) and C allele (OR=1.950) were risk factors for adverse pregnancy outcomes (P<0.05). Conclusion: The rs10865710 and rs4684847 locus of PPAR-γ gene and rs7020782 locus of PAPP-A gene may be associated with PE susceptibility and adverse pregnancy outcomes of PE. Detection of SNP locus of these two genes may be helpful to evaluate the risk of PE and adverse pregnancy outcomes. [ABSTRACT FROM AUTHOR]

[Objective] The analysis on grain filling characteristics and functional genes of early maturity wheat variety Chuanmai 1247 can provide reference for breeding new early maturity and high yield wheat varieties in Sichuan Basin in the future. [Method] Using early, middle and late maturity high yield wheat varieties widely planted in Sichuan Basin as materials, the Logistic equation was used to describe grain filling processes, and a series of grain filling parameters derived from it. The relationship between grain filling parameters and grain weight was analyzed by correlation analysis, and the functional genes of Chuanmai 1247 were detected by Wheat 50K SNP array. [Result] The whole growth duration of Chuanmai 1247 was 4-5 days earlier than that of middle maturity varieties widely planted in Sichuan, and 7 days earlier than that of the late maturity varieties. Compared with other varieties, Chuanmai 1247 had earlier heading date, shorter grain filling duration and higher average grain filling rate. Judging from the three stages of grain filling, the grain filling duration of the rapid and slowly increase periods of Chuanmai 1247 were shorter, while the grain filling rate of the gradual and slowly increase periods were higher. According to the changing trend of grain filling rate of wheat varieties with different maturity, early maturity varieties could keep fast grain filling rate, and the peak value of grain filling process was higher. The results of functional genotyping of Chuanmai 1247 by Wheat 50K SNP array showed that Chuanmai 1247 contained photoperiod sensitivity gene Ppd-D1a, vernalization genes vrn-A1 and Vrn-D1a, and genes TaSus2-2A, TaGASR-7A, TaGW2-6B, TaSus1-7B, TAGS1a and TaGS-D1a which positively controlled thousand kernel weight. The pyramiding effects of the above genes might be an important reason for excellent performance in early maturity and high yield traits of Chuanmai 1247. [Conclusion] Chuanmai 1247 had a high grain filling rate, which could make up for the adverse effects of shortening grain filling duration, and also agglomerated the excellent functional gene resources related to early maturity and high yield. Therefore, Chuanmai 1247 had high utilization value in current production and early maturity wheat breeding. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Acta Agriculturae Zhejiangensis is the property of Acta Agriculturae Zhejiangensis Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

To investigate the correlation between the transient receptor potential cation channel subfamily M member 3 (TRPM3) gene and duck egg laying trait, in this study, the TRPM3 gene of 90 Jingding ducks were sequenced by Illumina highthroughput sequencing to analyze the genotype frequencies of 6 SNP loci in TRPM3 and association of egg laying number and egg weight among different genotype ducks. The correlation between the mutation sites after forming haplotypes and the difference of egg laying number and egg weight was analyzed. The expression difference of TRPM3 mRNA in duck ovaries and oviducts with high and low egg laying number was detected by fluorescence quantitative PCR. The results showed that the mutation of SNPs of Z-36500134 T > C, Z-36503668 A > C, Z-36534782 G >A, Z-36684262C> T, Z-36710928 A > G, and Z-367324876 G > A of TRPM3 gene significantly reduced egg laying number and increased egg weight. There were four haplotypes of TRPM3 gene. The TAGCAG haplotype was the dominant haplotype, which was extremely positively correlated with egg laying number and extremely negatively correlated with egg weight. There was no significant difference in the expression of TRPM3 in the oviducts of the groups with high egg laying number and low egg laying number, and the expression of TRPM3 mRNA in the ovary in low egg laying number group was significantly lower than that in high egg laying number group. The results suggested that TRPM3 may play an important role in the egg laying performance of ducks, and affect the egg laying performance by affecting the ovarian function. [ABSTRACT FROM AUTHOR]