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Diabetes Mellitus is a metabolic disorder characterized primarily by chronic hyperglycemia. As the population ages, the prevalence of type 2 diabetes mellitus (T2DM) among the elderly is increasing, leading to a higher incidence of chronic complications, disability, and mortality associated with diabetes. This paper reviews the factors influencing chronic complications in elderly patients with T2DM and examines health behavior assessment tools for this demographic. The objective is to provide a reference for developing and implementing intervention plans and measures to address chronic complications in elderly T2DM patients, ultimately aiming to reduce the occurrence and progression of these complications.

Background Hepatic macrophages play a vital role in the defense mechanisms and maintaining the internal environment stability of body, and are also major cellular components involved in liver injury and repair. Macrophages derived from hematopoietic stem cells exhibit distinct gene regulation patterns compared to resident macrophages in the liver. More than 90% of primary liver cancer occurs on the basis of cirrhosis, and the dynamic changes of macrophages in the progression of cirrhosis to hepatocellular carcinoma are worth exploring. Objective To analyze the transcriptomic differences of hepatic macrophages originating from diverse sources, analyze the dynamic pattern of macrophage changes in liver cirrhosis and liver cancer progression, and explore potential strategies for preventing the progression of liver cancer. Methods In this study, single-cell transcriptomics data of healthy, cirrhotic and hepatocellular carcinoma (HCC) tissues were obtained from the Gene Expression Omnibus (GEO) database. The healthy and liver fibrosis data were obtained from the GSE136103 dataset of the GEO database, which included samples from five healthy liver tissues and five liver cirrhosis tissues. The HCC data were obtained from the GSE149614 dataset of the GEO database, which consisted of 21 samples from ten HCC patients. Utilizing the Seurat package, a clustering analysis was conducted on the transcriptomic data derived from liver fibrosis and HCC samples to identify distinct cell types. Notably, three distinctive clusters of macrophage subtypes were identified within the fibrosis samples, from which the top 200 marker genes were extracted. Metascape online analysis software was applied to functionally analyze each subcluster-specific expressed gene. Subgroup-specific expressed genes in liver fibrosis were extracted, and the function of macrophages in cirrhosis was explored by KEGG functional analysis. The CellChat software package was utilized to analyze intercellular interactions within liver fibrosis and HCC single-cell transcriptome data, differences in macrophage communication between cirrhosis and HCC samples were compared. Additionally, normal, fibrotic and cancerous macrophages were extracted, and batch effect correction was performed using the Harmony package. Subsequently, the Monocle package was employed for pseudo-time analysis to construct the developmental trajectory of macrophages spanning from a healthy state to fibrosis and eventually to the HCC microenvironment. The limma package was utilized to find genes that are continuously up-regulated and down-regulated during the evolution of macrophages from healthy state to cirrhotic state and finally to HCC, and functional enrichment analysis was performed. Results Unsupervised clustering was performed, and a total of three macrophage subclusters (designated as Mac1, Mac2, Mac3) were identified based on the expression patterns of marker genes. Mac1 originates from tissue-resident macrophages (Kupffer cells). Mac2 and Mac3 derived from blood monocytes and their numbers were significantly increased in cirrhotic tissue. Mac1 in cirrhotic tissue showed up-regulation of adaptive immune system-related functions. Mac2 and Mac3 subgroups show down-regulation of phagosome-related functions and antigen presentation functions. There were significant differences in communication between macrophages and other cell types in cirrhotic tissue and HCC tissue. Certain intercellular communication occurs only in cirrhotic macrophages, including cell communication of signaling pathways such as IFN-Ⅱ and CD40. After batch effect correction, pseudo-time series analysis was performed on macrophages from healthy liver, liver cirrhosis and HCC, the results suggest that there is a specific temporal relationship between the three groups of macrophages. This study identified 81 genes that were continuously down-regulated during the process, however, no genes were identified that were continuously up-regulated during the evolution of healthy-cirrhotic-HCC macrophage. Functional analysis suggested that the continuously down-regulated genes are functionally enriched for immune responses to bacteria. Conclusion Cirrhotic macrophages can be divided into three subgroups, of which Mac1 derived from liver-resident Kupffer cells and Mac2 and Mac3 derive from blood monocytes. Many immune-related cell communications in liver cirrhosis, such as IFN-Ⅱ and CD40 pathways, disappear in HCC. There is a continuous down-regulation of immune responses to bacteria in the evolution of healthy -cirrhotic-HCC macrophages, which may exacerbate the destructive effect of portal hypertension-induced gut microbiota displacement. For patients with liver cirrhosis, early treatment of portal hypertension-induced intestinal leakage (leaky gut) may be an important treatment strategy.

By using activated sludge from the anaerobic tank of a coking plant, a highly efficient phenol-degrading bacterial (PDB) community was successfully obtained through separation and domestication, and its effect on microbial degradation of phenol was investigated. The results showed that the degradation effect of PDB community on phenol was affected by the substrate concentration. The degradation rate reached 100% when the phenol concentration was lower than 700 mg/L. However, when the concentration of phenol increased to 1 300 mg/L, the degradation rate decreased to 41.6%. When the concentration of phenol exceeded 1 700 mg/L, the PDB community could basically unable to grow. High-throughput sequencing revealed that the species richness within the PDB community was high. Proteobacteria, Firmicutes, and Bacteroidota were dominant phyla, while Serratia sp., Bacillus sp., and Muribaculaceae sp. were core dominant genera of PDB. Metabolites analyzed by gas chromatography-mass spectrometry (GC-MS) revealed that the pathway for phenol degradation by PDB community was through ortho-cleavage, resulting in acidic intermediate products that subsequently decomposed into chain-like small molecular substances.

The thermal radiation of gaseous combustion products in an aero-engine combustion chamber has obvious non-ash-body characteristics. In this paper, based on the OpenFOAM platform, a numerical study on multi-field coupled turbulence-combustion-radiation radiative heat transfer was carried out for a certain type of aero-engine combustion chamber using the mean weighted sum of grey gas (WSGG) gas radiation model, which can accurately describe the non-ash characteristics of the gas. The results show that the radiation heat transfer characteristics are influenced by several factors such as equivalent ratio, combustion pressure and wall emissivity, among which the equivalent ratio is dominant. Increasing the equivalence ratio from 0.11 to 0.4 resulted in a 189% increase in average incident radiation at the combustion chamber wall and a 233% increase in average incident radiation at the exit. Increasing the equivalent ratio, combustion pressure or wall emissivity will increase the average radiation intensity in the combustion chamber and the inhomogeneity of radiation distribution at the exit of the combustion chamber. The radial radiation distribution factor (RRDF) also increases with the increase of equivalent ratio, combustion pressure and wall emissivity.

In 2024, the Chinese Meridian Project (CMP) completed its construction, deploying 282 instruments across 31 stations. This achievement not only provides a robust foundation but also serves as a reference template for the International Meridian Circle Program (IMCP). The IMCP aims to integrate and establish a comprehensive network of ground-based monitoring stations designed to track the propagation of space weather events from the Sun to Earth. Additionally, it monitors various disturbances generated within the Earth system that impact geospace. Over the past two years, significant progress has been made on the IMCP. In particular, the second phase of construction for the China-Brazil Joint Laboratory for Space Weather has been completed, and the North Pole and Southeast Asia networks are under active construction. The 2024 IMCP joint observation campaign was successfully conducted. To facilitate these developments, the scientific program committee of IMCP was established, following the success of 2023 IMCP workshop and the space weather school, which was co-hosted with the Asia-Pacific Space Cooperation Organization (APSCO) and sponsored by Chinese Academy of Sciences (CAS) and Scientific Committee on Solar-Terrestrial Physics (SCOSTEP). Preparations are now underway for the 2024 workshop in collaboration with the National Institute for Space Research (INPE) in Brazil.

Iron-loaded coal gasification fine residue activated carbon (Fe/MAC) catalysts were prepared by the impregnation-calcination method using inexpensive coal gasification fine residue (CGF) as a carrier. The removal of simulated phenol from wastewater was experimentally investigated by activated carbon (Fe/MAC) catalysts with Fe-unloaded CGF activated carbon (MAC) as control. The catalysts were characterized by scanning electron microscopy (SEM), specific surface area and micropore analyzer (BET) and X-ray photoelectron spectroscopy (XPS). The effects of reaction times, reaction temperatures and catalyst dosages on phenol removal rate were systematically studied. The results showed that the iron was successfully loaded onto MAC by impregnation-calcination method, and the optimal conditions for phenol removal in the heterogeneous Fenton system based on Fe/MAC/H2O2 were 0.35 g of Fe/MAC catalyst dosage, 5 h of reaction time and 70 ℃. The results of this study provide a new idea for alleviating the secondary pollution of wastewater and catalyst separation of iron in the traditional Fenton method, expande the high-value application of coal gasification fine residue, and provide a new way for the efficient treatment of phenol wastewater.

ObjectiveTo investigate the pollution status of heavy metal contamination in commercially available grains in Wuxi， and to access the potential risk to humans.MethodsThe grains such as rice， flour and coarse cereals sold in Wuxi were collected， and the contents of Pb、Cd、Hg、As were detected according to the national standard method. The single factor pollution index and Nemero multi-pollution index were used to evaluate the pollution status of heavy metals in grains， and the environmental health risk assessment model recommended by the U.S. Environmental Protection Agency （US EPA） was used to evaluate the health risk caused by heavy metals through diet.ResultsThe single factor Pollution Indexs of rice， flour and coarse cereals were all less than 0.6， which is within the safe range. The carcinogenic risk value of grains is 1.71×10-4， which is within the acceptable risk level recommended by US EPA， and rice is the major exposure media. The non carcinogenic hazard quotient （HQ） of all four metals were all less than 1，which were decreased in the order of As>Cd>Hg>Pb. All the non carcinogenic hazard index （HI） was less than 1 too， which were decreased in the order of rice> flour>coarse cereals.ConclusionThe heavy metal contamination of grains in Wuxi is within the acceptable range， and the health risk assessment showed that carcinogenic risk value of grains was within the acceptable risk level recommended by US EPA. The main carcinogenic and noncarcinogenic risk all come from rice， and the attribution factor is arsenic.

[Objective] The study on the spatial-temporal change characteristics of land use/cover is of great significance for the protection and rational development of land resources in the Yellow River Basin, and can provide an important reference for the implementation of ecological protection and high-quality development strategy in the Yellow River Basin. [Methods] Based on the annual land use/cover data of the Yellow River Basin from 1980 to 2021, the characteristics of land use/cover change in the Yellow River Basin in recent 40 years were investigated by using Mann-Kendall and sliding t-test, geographic information TUPU standard deviation ellipse and gravity shift model. [Results] (1) From 1980 to 2021, forest land, shrub, water area, and construction land increased significantly, while the cultivated land, grassland, and wetland decreased significantly. (2) The periods of land use/cover change in the basin can be divided into 1980—1992, 1993—2001, 2002—2012, and 2013—2021, and the quantitative structure gradually presented by rapid changes, and the changes of construction land, wetland and water area were sensitive. The balance of land-use structure was gradually increased, and the stage changes showed a high correlation with the implementation of major strategic measures, and more consistent with the divided stage. (3) In the middle and upper reaches of the basin, the main changes were from 2012 to 2021 and intermittent changes, and the main changes were from 2001 to 2012 and from 2012 to 2021, and the main changes were from 2001 to 2012 and from 2012 to 2021, and the main changes were from arable land to construction land and grassland. The basin changed frequently in northern Ningxia, eastern inner Mongolia and some areas along the Yellow River system. (4) The centers of gravity of wetland, water and construction land moved westward, showing concentrated distribution to the southwest, east and northwest, respectively. [Conclusion] The overall trend of land use structure becomed better under the guidance of policies. In the future, we should pay more attention to wetlands and areas with frequent land use changes.

Postoperative pain, a common problem in perioperative management, has negative impact on patients' experience and functional recovery. The development of postoperative pain is not only related to direct surgical trauma, but also a series of preventable perioperative risk factors, among which perioperative sleep quality should not be neglected. In recent years, more and more researches have indicated that poor perioperative sleep quality is a risk factor for both acute and chronic postoperative pain, and some researches have even pointed out that sleep quality can directly influence patients' pain threshold. Therefore, this review aims to summarize current research progress on the influence of perioperative sleep quality on postoperative pain, hoping to provide reference for improving the perioperative pain management for patients.

Based on the concept of ecosystem service value, this study proposes a method for estimating the net value of ecosystem services considering cost inputs, and estimates and analyzes the net value of ecosystem services in the Yellow River Estuary wetland from 2019 to 2022 through time series remote sensing monitoring. The results indicate that:(1) Among the 11 types of ecosystem services in the Yellow River Estuary coastal wetland, biodiversity, carbon sequestration, food supply, and wave attenuation and protection are the dominant ecosystem services. The nearshore waters, reed beds, salt-alkali plants, and tidal flats show strong net value capacity. The net value of ecosystem services in 2022 decreased by 4.08×108 yuan compared with that in 2021, mainly due to the disappearance of a large area of salt-alkali plants with strong net value in 2022. (2) Despite the negative net benefits observed in the first year after the construction of typical ecological restoration projects in both 2019 and 2020, the net value of ecosystem services has reached the expected level. (3) Sensitivity analysis demonstrates the significant role of wave attenuation and protection, food supply, carbon sequestration, and biodiversity among the various ecosystem services. The estimation of net value of ecosystem services enables the comparison and analysis of the maximum net value generated by different types of wetlands, providing a scientific basis for the rational planning and management of coastal wetland ecosystems.

Postoperative pain seriously affects the recovery process of patients, resulting in prolonged hospital stay and increased care costs. Appropriate application of patient-controlled analgesia devices can effectively relieve perioperative acute pain. In 1994 patient-controlled analgesia began to be used in Peking Union Medical College Hospital, and the Acute Pain Service Working Group was established in 2004. With the cooperation of anesthesiologists and specialist nurses, the group jointly has implemented the whole process and standardized management based on patient-controlled analgesia, and constantly improved and innovated working methods, laying a solid foundation for the development of postoperative pain management. This paper systematically reviews and summarizes the work from the aspects of clinical focus, nursing management experience, promotion and dissemination of pain treatment concepts, and development of acute pain service model under the new situation, with the hope of providing valuable reference for comprehensively strengthening pain management in the process of diagnosis and treatment, and enhancing patients' satisfaction with perioperative analgesia services.

Objective To establish a health promotion team consisting of nurses, and to carry out health promotion programs to improve the health care knowledge of patients in the outpatient clinic. Methods Health promotion programs including health education lectures, nursing health counseling, handbook of health-related knowledge and self-made videos were carried out, and the quality of the activities was continuously improved according to the evaluation outcomes. Results Participants’ awareness of disease prevention and control had been enhanced after implementation of health promotion programs(P＜0. 05). Conclusion By taking advantage of workplace competence of nurses, nursing health promotion programs are effective to improve the disease prevention and control knowledge and self-protection ability of the population who had visited the outpatient clinic. (目的 通过组建护理健康科普队伍, 传播护理科学健康知识, 提高就诊患者的健康保健知识水平。方法 利用早、午间候诊时间段开展护理健康科普讲座、护理健康咨询、传递健康知识手册及自媒体视频等方式进行健康科普工作, 根据评价结果不断改进活动质量。结果 干预后, 参加护理健康科普讲座的受众者对相应讲座的健康知识掌握度优于干预前, 差异有统计学意义(P＜0. 05)。结论 通过开展护理健康科普, 发挥护士在健康科普工作中的专业优势, 有助于提高受众者的疾病防治知识水平和自我防护能力。)

ObjectiveTo investigate the effect of high-frequency repetitive transcranial magnetic stimulation （rTMS） on postoperative cognitive dysfunction （POCD） in aged mice，and analyze its mechanism.MethodsA total of 24 18 month old C57BL/6J male aged mice were randomly divided into control group，operation group and rTMS group，with 8 mice in each group. The rTMS group received 5 Hz rTMS true stimulation for 10 consecutive days before operation，and underwent experimental laparotomy. The rTMS true stimulation continued for 4 days after operation，once a day，the magnetic stimulation intensity was 0. 84 T，the frequency was 5 Hz，100 pulses per sequence，the interval time was 30 s，and 10 sequences were repeated，a total of 1000 pulses. The control group and the operation group received sham stimulation for 10 days before operation，the control group did not operate，the operation group underwent experimental laparotomy，and the control group and the operation group received sham stimulation for 4 days after operation. On the third day after operation，the locomotor activity of mice was evaluated by open field test （percentage of stay time in the central area，total distance and average speed of movement）; On the fourth day after operation，the cognitive function of mice was evaluated by Y-maze test （the percentage of times to enter the new arm，the percentage of time to stay in the new arm，and the total distance to move the new arm）; After the Y-maze test，the protein expression levels of brain-derived neurotrophic factor （BDNF），tropomyosin related kinase B （TrkB） and nerve growth factor （NGF） in mice hippocampus were detected by immunoblotting.Results（1） Exercise capacity：there was no significant difference in the percentage of time spent in the central area of the open field，total distance and average speed among the three groups （P>0. 05）. （2） Cognitive function：there was no significant difference among the three groups in the percentage of times of exploring new forearm （P>0. 05）; Compared with the control group，the percentage of stay time in the new arm and the total distance of new arm movement in the operation group were significantly reduced，and the differences were statistically significant （PPPP

With the arrival of an aging society, the number of orthopedic geriatric patient who require surgical treatment is also rapidly increasing. Elderly patients have decreased hematopoietic function, and perioperative blood management is difficult and challenging. This article aims to elaborate on the perioperative blood management strategies for orthopedic geriatric patients, including the diagnosis and treatment of preoperative anemia, specific measures to reduce perioperative blood loss, and blood transfusion strategies.

Objective To investigate the effect of palliative care in pain management of terminally ill patients. Methods A total of 50 terminally ill patients with pain were divided into the control group and observation group according to the admission sequence, with 25 cases in each group. Patients in the control group received routine nursing and pain management, and those in the observation group were given palliative care interventions in addition. The degree of pain was evaluated using a Visual Analogue Scale(VAS). Results The VAS score was decreased in both two groups after intervention, and VAS score in the observation group was lower than that in the control group (P＜0. 01). Conclusion Palliative care is effective to reduce the pain of terminally ill patients, and it helps to improve the quality of life in terminal stage. (目的 探讨安宁疗护在临终患者疼痛管理中的应用。方法 选取医院老年科2021年1月—2022年12月收治的50例临终患者为研究对象, 均伴有不同程度的疼痛。根据入院时间将患者分为观察组和对照组, 各25例。对照组采用常规护理和疼痛管理, 观察组在对照组基础上增加安宁疗护, 对比两组患者疼痛视觉模拟量表(VAS)评分。结果 干预后两组VAS量表评分降低, 且观察组VAS量表评分低于对照组, 差异有统计学意义(P＜0. 01)。结论 安宁疗护能有效缓解临终患者疼痛, 有助于改善生存质量。)

Sterilization is an indispensable part of food processing. This paper discusses the theories and applications of four currently widely used non-thermal sterilization technologies， including dense phase carbon dioxide， electron beam irradiation， pulsed light， and sonodynamic sterilization. Compared with traditional sterilization methods， these four technologies offer advantages in color maintenance， preservation of food flavor and nutrients， economy of energy， enhanced safety， and expense savings. Therefore， the development of new technologies will provide a frame of reference for choosing the most suitable sterilization technique to best maintain the quality of each kind of food.

BackgroundThe incidence of delirium in critically ill psychiatric patients is high， and there are many factors affecting delirium occurrence. At present， epidemiological studies on delirium among critically ill patients in psychiatric hospitals are limited.ObjectiveTo explore the risk factors for delirium in critically ill patients in a psychiatric hospital， so as to guide the clinical management of delirium in psychiatric hospitals.MethodsThis retrospective study included 427 critically ill patients who were admitted to Shenzhen Kangning Hospital from January 1， 2019 to May 31， 2021. The delirium situation， gender， age， pre-admission course of illness （duration from the onset of acute mental state changes to in-patient registration at a psychiatric hospital）， history of mental illness， history of cognitive dysfunction， history of using psychoactive substances， history of using sedative and hypnotic drugs， number of combined chronic diseases， number of combined drugs and type of disease were examined as potential risk factors for delirium. Single Logistic regression was used to analyze the potential risk factors for delirium， and the potential risk factors were incorporated into the multi-factor Logistic regression analysis model so as to gradually screen out the risk factors for delirium in critically ill psychiatric patients.ResultsDelirium was present in 33.49% （143/427） of critically ill patients. Multi-factor Logistic regression analysis demonstrated that the presence of delirium was associated with mental and behavioral disorders caused by psychoactive substances （OR=8.949， P

Objective·To establish an in vitro culture model mimicking tumor microenvironment using hydrogel microspheres and fresh primary pancreatic cancer cells.Methods·The morphological distribution of the hydrogel microspheres was recorded, observed and photographed under an inverted fluorescence microscope. The diameter of the microspheres was calculated by Image J, and the particle size distribution map was obtained by statistics. Renal epithelial cells (293T), pancreatic cancer cells (8988), and normal pancreatic epithelial cells (HPNE) were grown in DMEM complete medium, and passaged when the cells were 80%‒90% confluent. 293T cells were cultured in DMEM medium and microsphere extract, and the proliferation curve of 293T cells cultured in the two mediums was detected by CCK-8 method to explore the biocompatibility of hydrogel microspheres. Fresh pancreatic tumor tissue was cut in the ultra-clean workbench, and pancreatic cancer tumor tissue was lysed by hyaluronidase and collagenase Ⅰ, and digested into single cell with interval shaking in a 37 ℃ water bath. The hydrogel microspheres and pancreatic cells were co-cultured in DMEM complete medium for 3 d, 1/2 of the cells were fixed with 4% paraformaldehyde, and stained with phalloidin and DAPI, and the morphology and cell distribution of the microspheres were observed under a common fluorescence microscope. The remaining 1/2 cells were used for suspension and adherent cell counting. The hydrogel microspheres and primary cells were co-cultured in the medium for 7 d, and the cell composition in the established culture model of in vitro pancreatic cancer primary cells based on the hydrogel microspheres was observed by immunofluorescence method. The pancreatic cancer tissue was embedded in paraffin, followed by paraffin tissue sectioning, hematoxylin and eosin staining of the tissue section, and observation of the pancreatic cancer tissue structure with a microscope.Results·The size of the hydrogel microspheres was uniform, and the diameter of the microspheres was about 200 μm. The proliferation curve of 293T cells showed that the hydrogel microspheres had good biocompatibility. The co-culture experiments of hydrogel microspheres and pancreatic cell lines showed that the surface of hydrogel microspheres had strong cell affinity, which could provide support points for pancreatic cells to adhere to the surface of the microspheres and to grow normally. Through co-culture of hydrogel microspheres with fresh pancreatic cancer cells, a 3D culture model of pancreatic cancer cells in vitro was successfully established. Composition of cell types in this model was similar to that in the corresponding primary tumor tissue, which included pancreatic ductal epithelial cells, tumor stem cells, endothelial cells, fibroblasts and other cells.Conclusion·The in vitro 3D culture model of primary pancreatic cancer cells based on hydrogel microspheres has important characteristics of pancreatic cancer tumor microenvironment.

To clarify the conditions and furanone production by Zygsoaccharomyces rouxii, the fermentation conditions by Z. rouxii QOR6 were optimized through single factor and orthogonal experiments. Then the types and relative contents of volatile aroma substances were determined by gas chromatography-mass spectrometry (GC-MS). The results showed that the fermentation temperature of 28 ℃, the initial pH of 4.2, the incubation speed of 180 r/min and the inoculation amount of 20×107 CFU/mL were the optimal conditions, in which the yield of HDMF reached 2.90 mg/L, 1.09 times higher than that of before optimization. There were 41 volatiles components in the fermentation broth, mainly including alcohols, aldehydes, esters, aromatics and hydrocarbons. Among them, alcohols indicated the highest content, accounting for 81.13% of the total volatiles. Other metabolites with higher relative contents were phenethylacetate (3.48%), formaldehyde (0.34%), 2,6-di-tert-butylphenol (8.77%), chloromethoxymethane (0.20%) and 3-ethyl-2,5-methylpyrazine (0.70%). This study might provide technical support for the application of the Z. rouxii in food.

Objective To investigate the inductive effect of human adenovirus type 7 (HAdV-7) infection on ferroptosis in alveolar epithelial cells. Methods Human alveolar epithelial carcinoma cells A549 and human pulmonary type Ⅱ alveolar epithelial cells HPAEpiC were infected with HAdV-7, respectively, and in 24, 48 and 72 h after infection, cell viability was measured using CCK-8 assay, mitochondrial morphology was observed by transmission electron microscopy (TEM), productions of reactive oxygen species (ROS) and malondialdehyde (MDA) were measured with ROS probe and Lipid Peroxidation, respectively, and intracellular Fe2+ level were observed by Fe2+ fluorescence probe. RT-qPCR and Western blotting were applied to determine the expression changes of key genes in ferroptosis. After CD46 humanized mice were infected with HAdV-7 by nose dropping, Perls staining and Western blotting were applied to detect iron deposition and the expression of ferroptosis-related proteins in the lung tissue. After administration of ferroptosis inhibitor, deferoxamine mesylate (DFO), in the mice with HAdV-7 infection, CCK-8 assay and HE staining were performed to observe the alveolar epithelial cell death and lung pathology. Results After HAdV-7 infection, cell viability were reduced in the A549 and HPAEpiC cells, and smaller mitochondria and decreased even disappeared mitochondrial crista were observed in the ferroptotic cells. ROS level (A549 and HPAEpiC cells: 48 h and 72 h, P < 0.01) and MDA level (both A549 and HPAEpiC cells: P < 0.05) were significantly higher in the HAdV-7 group than the control group. The intracellular iron concentration was obviously higher in the infected HPAEpiC cells than the control cells (P < 0.01). RT-qPCR results showed that the mRNA levels of cystine/glutamate antiporter solute carrier family 7 member 11 (SLC7A11, also known as xCT) (P < 0.01), glutathione peroxidase 4 (GPX4) (P < 0.05), ferroportin (SLC40A1) (P < 0.05) and ferritin heavy chain (FTH1) (A549 cells: 72 h, P < 0.01; HPAEpiC cells: 48, 72 h, P < 0.01) were down-regulated after HAdV-7 infection, whereas that of HAMP was up-regulated (A549 cells: 72 h, P < 0.01; HPAEpiC cells: P < 0.05). The protein levels of xCT and GPX4 were also decreased in the infected HPAEpiC cells than the control group. HAdV-7 infection in the CD46 humanized mice resulted in deepened iron stains in the lung tissue, and decreased protein levels of xCT (P < 0.05) and Gpx4 (P < 0.01). Ferroptosis inhibitor DFO could partially prevent HAdV-7-induced cell death (HPAEpiC: P < 0.01), and obviously enhance Fer-1 inhibited cell death (A549 cells: P < 0.05; HPAEpiC cells: P < 0.01). In addition, Fer-1 treatment could effective ameliorate pathological damages in the CD46 humanized mice after HAdV-7 infection. Conclusion HAdV-7 infection increases iron accumulation and lipid peroxides, and thus induces ferroptosis in alveolar epithelial cells, which may be involved in lung injury caused by HAdV-7 infection.

Objective To investigate the effect of predictive nursing strategies on preventing side effects of azacitidine in patients with high-risk myelodysplastic syndrome. Methods Totally 40 patients undergoing subcutaneous injection therapy with azacitidine for high-risk myelodysplastic syndrome were randomly divided into the observation group and control group, with 20 cases in each group. Routing nursing management including posture nursing, oral care, psychological care and nursing of adverse reactions was carried out in the control group. In the observation group, a special nursing team was set up to enhance the predictive nursing interventions such as nutrition support, prevention of infection and hemorrhage, and so on. The side effects of azacitidine therapy were observed analyzed. Results The incidence of adverse reactions in the observation group was significantly lower than that in the control group (10. 00% vs. 45. 00%, P＜0. 05). Conclusion For those patients undergoing subcutaneous injection therapy with azacitidine for high-risk myelodysplastic syndrome, the predictive nursing is potentially effective to reduce the incidence of adverse drug reactions. (目的 探讨预见性护理干预预防较高危骨髓增生异常综合征患者阿扎胞苷治疗所致不良反应的效果。方法 40例较高危骨髓增生异常综合征患者随机分为观察组(n=20)和对照组(n=20)。两组患者均给予阿扎胞苷皮下注射治疗。对照组实施常规护理, 包括体位护理、口腔护理、心理护理和不良反应护理; 观察组实施预见性护理干预, 成立预见性护理小组, 强化营养干预、感染预防、出血预防等护理措施。比较两组不良反应发生情况。结果 观察组不良反应发生率10. 00%(2/20), 低于对照组的45. 00%(9/20), 差异有统计学意义(P＜0. 05)。结论 对较高危骨髓增生异常综合征患者, 应用阿扎胞苷治疗的同时配合预见性护理, 可以有效降低不良反应的发生风险。)

Evapotranspiration is an important link to water and heat exchange between soil and atmosphere， and its changes have an important impact on water resources management and ecological environment.In the context of global warming， the permafrost on the Qinghai-Xizang Plateau is continuously degraded， and the near-surface hydrothermal conditions in the permafrost regions have changed， which further affect the evapotranspiration process.In this paper， four semi-empirical models based on the complementary principle of evapotranspiration are selected to evaluate the ability of different models to simulate the actual evapotranspiration on a daily scale before and after calibrating parameters， and also analyze the sensitivity of the model parameters， which using the meteorological and eddy covariance measurements from January 1， 2010 to December 31， 2011 at the Tanggula comprehensive observation site located in the hinterland of the permafrost region of the Qinghai-Xizang Plateau.The results show that the simulated actual evapotranspiration is very sensitive to the value of parameter α in the models， unreasonable parameter values led to poor simulation ability.Specifically， the use of default parameter values will lead to marked higher simulated values of daily evapotranspiration， the S2017 model has the highest simulation accuracy with RMSE value of 0.44 mm·d-1.After parameters calibration， all models can simulate variation characteristics of daily actual evapotranspiration， RMSE values are between 0.3 and 0.4 mm·d-1， the model with the highest simulation accuracy is the C2016 model， followed by H2018 model， B2015 model and S2017 model， in comparison， evapotranspiration simulated by H2018 model exhibits more stable results， which indicates lower parameter dependency.In addition， accuracy of simulation exists significant difference during various period within the year， and the simulation results of the thawed period are closer to the measured values than those of the frozen period and the daily freeze-thaw cycle period， mean relative deviation values are 6.0%， 82.3%， 43.4%， respectively.The purpose of this paper is to explore the applicability of different complementary evapotranspiration models to the simulation of actual surface evapotranspiration in the permafrost region of the Qinghai-Tibet Plateau， and to some extent， the results can provide reference basis for the estimation of long time series evapotranspiration in this region.

Microcrystalline graphite, also known as amorphous graphite, is named for its small crystal structure. The particle size of the microcrystalline graphite is small, and the flotation foam is easy to physically polymerize with the fine gangue during fine grinding and purification, resulting in unsatisfactory flotation and purification effect. Especially because the higher the fixed carbon content is, the higher the viscosity of the flotation foam is, the heavier the physical entrainment in the foam product, resulting in the current production problems such as long selection process, low product quality, and high production power consumption.In this paper, the variation rule of half-life of flotation froth under different dosage of sodium chloride and 2# oil is compared and studied. It is confirmed that adding sodium chloride can improve the flotation index of microcrystalline graphite, and is verified by actual ore. The research results can provide reference for industrial application and popularization of microcrystalline graphite flotation.

Objective To observe the effect of auricular acupoint sticking and pressing therapy on preventing postoperative nausea and vomiting in patients undergoing thoracolumbar surgery under general anesthesia. Methods To retrospectively analysis the clinical data of 126 patients who underwent posterior thoracolumbar surgery under general anesthesia, including 37 patients who underwent auricular point sticking and pressing 24 hours before surgery (group A), 48 patients who underwent auricular point sticking and pressing after surgery(group B) and 41 patients without auricular acupoint sticking and pressing therapy after operation (group C). Incidence of postoperative nausea and vomiting and PONV scores were recorded at 8 h, 24 h and 48 h after operation. Results Among the three groups of patients, group A had the lowest incidence of postoperative nausea and vomiting, and the lowest PONV score. The incidence of PONV at 48h after operation was 35. 13%, which was significantly lower than that in the group C(P

Based on the tunable diode laser absorption spectroscopy (TDLAS) technology, one-dimensional and multi-dimensional combustion parameter monitoring systems were designed to simultaneously monitor multiple combustion field parameters in the furnace (such as temperature, CO, CO2, and NO concentration, etc.). In the one-dimensional system, the optical switch technology was applied to measure the multiple combustion parameters simultaneously. In the two-dimensional system, the spectrometer technology and algebraic reconstruction technique (ART) were introduced to inversion calculate and measure the two-dimensional combustion parameters in a layer. On this basis, the three-dimensional system was designed. The measurement procedures of three-dimensional combustion parameters were as follows: the combustion region in the furnace was divided into multilayer sections; the two-dimensional combustion parameters were reconstructed based on ART; the three-dimensional combustion parameters were reconstructed using the multilayer combustion parameter data. The combustion filed parameter monitoring system contributes to the fine combustion adjustment in the future.

Objective To evaluate the safety and effectiveness of Perioperative Transfusion Trigger Score (POTTS) in guiding surgical patients blood transfusion intraoperatively and postoperatively. Methods A total of 900 patients(perioperative Hb 60~100 g/L) from December 2017 to March 2021 were collected, including 251 males and 649 females, with ASA grading Ⅰ~Ⅳ, and randomly divided into experimental group and controls. In the experimental group, the allogeneic RBC transfusion trigger(Hb threshold) and transfusion units in anemia patients was determined by POTTS. While those in the controls were decided by physicians according to current guidelines concerning transfusion. The proportion and units of allogeneic RBC transfusion, the incidence of postoperative complications, the mortality of hospitalization and discharge for 4 weeks, Hb value, healing of surgical incision, ICU admission rate and ICU length of stay, length of hospitalization, etc were recorded. Results The proportion of allogeneic RBC transfusion in the experimental group(35.3%)were less than the controls(42.2%)(P

In-situ monitoring of the dynamic contact force between catenary and pantograph is very important for the quality evaluation of pantograph catenary current collection. A monitoring method based on strain response was proposed to invert the value and position of the dynamic contact force between catenary and pantograph. The measurement principle, sensor installation method and calibration experiment of key parameters were described in detail. The monitoring system has been successfully applied to a metro test project with rigid overhead catenary. And the dynamic contact force between pantograph and catenary was monitored and analyzed when the subway was running at the speed of 60 km/h and 80 km/h, respectively. The results indicated that the dynamic contact force measurement method based on strain response has the advantages of high measurement accuracy, good data consistency and flexible sensor installation. It can not only accurately restore the value of dynamic contact force between catenary and pantograph, but also locate the position of the dynamic contact force on the carbon slide of pantograph. This work can effectively evaluate the performance quality of the coupling system of pantograph and catenary.

【Objective】 Organic fertilization and soil conditioner have been found capable of improving soil structure and enzymatic activity. The aim of this paper is to study how organic fertilizer and soil conditioner combine to regulate bioavailable nitrogen (N) and the yield of greenhouse tomato. 【Method】 The experiment was conducted in a solar greenhouse. It comprised five treatments: no fertilization (CK), chemical fertilization only (H), organic fertilization only (Y), 50% chemical N fertilizer and 50% organic (N) fertilizer (HY), 50% chemical N fertilizer + 50% organic N fertilizer + soil conditioner (HYG). In each treatment, we measured the change in mineral N, total N, N in microbial biomass, urease and nitrate reductase activity, and the tomato yield. 【Result】 Compared with CK, all fertilization treatments increased ammonium and nitrate in soil significantly, regardless of the growth stage. At harvest stage, HY increased total soil N and N in microbial biomass by 125.07%~204.22% and 289.20%~398.04% respectively, compared to those in the CK, 53.45%~79.80% and 133.74%~271.32%, respectively, compared with those in H. In all treatments, urease activity in the 0~20 cm soil peaked at the one ear fruit stage, while soil urease activity in the 20~40 cm soil maximized at the harvest stage. HY significantly increased nitrate reductase activity in the soil which peaked at the full fruit stage. Among all fertilization treatments, the activity of urease and nitrate reductase was maximum in Y and the least in CK. Compared with CK, all fertilizations increased tomato yield at significant level, with the yield increase ranging between 11.05% and 38.18%. Compared with H, HYG increased the yield by 38.18%. 【Conclusion】 Combination of 50% chemical N fertilizer and 50% organic N fertilizer reduced the costs but increased soil fertility and tomato yield without increasing N loss. It can then be used as an improved agronomic management for greenhouse tomato production.

This paper constructs an evaluation index system for high-quality development of coal industry from six dimensions: economic benefits, innovative development, intensive development, safe development, green development and efficient development.The high-quality development of China's coal industry from 2000 to 2019 is measured and analyzed in depth by entropy method, TOPSIS method and coupling coordination model.The results show that the high-quality development of the coal industry has achieved significant achievement, but the industrial scale is obviously out of sync with the high-quality development level.The main motivation and "short board" of high-quality development of the coal industry is significantly different in different stages of development.The six dimensions of high-quality development of the coal industry have been improved to varying degrees during the research period, but the speed of green development and high-efficiency development is relatively low.The coupling and coordination degree of the six-dimensional development of the coal industry has steadily increased, but has not yet entered the stage of "high-quality coordinated development"and the level of coordinated development needs to be improved.

We report new geochronological data of the mafic gneiss and leucogneiss from the Windmill Islands, East Antarctica, in order to unravel the tectonothermal events related to the amalgamation of Rodinia. SHRIMP zircon U-Pb dating from the mafic gneiss (Hbl-Cpx-Opx-Bt-Pl-Qtz-Mag-Zrn) yielded early Mesoproterozoic magmatic ages of 1403±28 Ma from igneous cores, and middle Mesoproterozoic metamorphic ages of 1318±34 Ma from overgrown rims. The leucogneiss (Pl-Kfs-Qtz-Bt-Zrn) in the Bailey Peninsula has intrusive ages of 1257±51 Ma from magmatic origin zircon cores, and metamorphic ages of 1197±26 Ma from overgrown rims and/or structureless grains. The intrusive age of mafic gneiss indicates the existence of a ca.1.40 Ga igneous activity in the Windmill Islands. This is likely the earliest igneous record of the Windmill Islands, possibly relating to the final period of igneous activity of the Mawson Continent. The age of high-grade metamorphism of the mafic gneiss from the Bailey Peninsula can be constrained by the metamorphic zircon overgrowth at 1318±34 Ma, suggesting that the Windmill Islands was possibly involved in the Albany-Fraser-Windmill (East Antarctic) orogeny during the 1375~1151 Ma period. This study further supports the tectonic model in which the Windmill Islands and the Albany-Fraser Orogeny are parallel convergence during the Mesoproterozoic Rodinia amalgamation.

Fe-Ti oxide minerals can be locally aggregated or enriched in the quartzofeldspathic gneisses from the Larsemann Hills, East Antarctica. The enrichment is related to anatexis and subsequent high-grade metamorphism, not inherited from the protolith. The partial melting process was responsible for the residues or local enrichment of the inert Fe-Ti-Al elements and the migration of mobile components, and the volatiles were preferentially incorporated in the melt. In the water-deficient system, the local "melt" in anatexis crystallized to form the leucosomes without melt texture or minimum eutectic composition, suggesting the meta-melt feature, not real melt. The coarse leucosome or pegmatite occurred in anataxis as pre-granitic vein or body and had nothing to do with the late stage differentiation of the granitic magma. Together with the presence of the meta-melt, the substantial differentiation of the components resulted in solid residues and corresponding meta-melt phases, with the former enriched in Al, Fe elements and sillimanite, Fe-Ti oxide minerals occurred. On the contrary, the leucosomes with possible short distance migration of components were poor in Fe, Ti compositions, and seldom formed garnet and Fe-Ti oxides. The volatile-unsaturated anatexis was an essentially closed system and the total dehydration effect was not obvious. The presence of some typical minerals in high-grade metamorphism of the quartzofeldspathic gneisses, such as sillimanite, garnet, cordierite and spinel, was derived from component differentiation in partial melting of the rocks. However, the components migration was limited, and the evolving stages of metamorphism and differentiation could be preserved. Voluminous Fe-Ti oxides and sillimanite in the quartzofeldspathic gneisses suggest the possible local or differential uplifting of the area, the heterogeneity of deformation and accompanying components differentiation, corresponding to the reactivation of the earlier structures in the Pan-African event.

The Archean cratonic nuclei in the East Antarctic Shield (Craton) occur mainly in the Napier Mountains, southern Prince Charles Mountains, Rauer Group and Vestfold Hills in the Indian Ocean sector, and are sporadically exposed in the Australian, African and Pacific sectors. These ancient nuclei with diverse earlier crustal histories and later reworking processes are separated by the Paleoproterozoic-Early Paleozoic (Pan-African-aged) orogens. The nuclei in different sectors have a close affinity with the adjacent Gondwana continental blocks. Integrated bedrock and subglacial geological investigations and petrological and chemical studies will ascertain the temporal and spatial distributions, petrogenesis, source regions, tectonic affinities and multiple metamorphic records of the Archean rocks (materials) in East Antarctica.This can help to reveal the major history from nucleation to assembly of the East Antarctica continent, and thus to contribute to a better understanding of the early history of the Earth from an Antarctic perspective.

Rapid digestion-standard addition based on graphite furnace atomic absorption spectrometry(GFAAS) for determining cadmium in grain of four kinds of matrix (brown rice, corn, wheat and rice) is established, by studying on digestion conditions, optimizing the proportion of sample and nitric acid, and using digested sample to prepare standard solution. The results show that the correlation coefficients are 0.995 to 0.999. The detection limit of method is 0.003 mg/kg. The relative error between the test result and the standard value is –3.8% to –1.1%. The standard recoveries of cadmium in low, medium and high gradient are 99.1% to 103.4%, and the RSD values of the standard substances and real samples are 1.2% to 2.7%.Compared with the results of microwave digestion method, the detection values of cadmium have no significant difference (P>0.05). The method have shown advantages in terms of accuracy and precision, and it is a rapid and accurate method to calculate cadmium in large quantities of grain samples.

In order to effectively solve the problem of interlaced overlap in the fundus image lesions, large and small blood vessels and severely affected by light, and to achieve multi-label classification of fundus images, in this paper, a single population frog-leaping optimization convolutional neural network algorithm (SFCNN) is proposed to detect various fundus lesions. The algorithm retains the efficient searching ability of the shuffled frog leaping algorithm (SFLA). It is simplified into a single population frog-leaping algorithm and effectively combined with the traditional convolutional neural networks (CNN). When initializing the network, the initial weight of the network is optimized by the frog-leaping algorithm. In the process of network iteration, the forward propagation loss of convolutional neural network is monitored and the abnormal weight is corrected by using the optimization ability of frog-leaping algorithm. After the network meets the end conditions, the final weight is optimized by frog-leaping, which further optimizes the network weight, so as to realize the detection and classification of complex fundus image with multiple lesions. The experiment of the detection of fundus image lesions shows that compared with CNN algorithm, the accuracy of the proposed algorithm is improved in both single lesion detection and overall detection.

After occurrence of natural disasters,active distribution network (ADN) can promptly restore power supply to some critical loads through tie-line switching and flexible distributed generation (DG),and thus the fault risk is effectively mitigated. A data-driven multi-dimensional intelligent forecast approach for the fault risk levels in ADNs is proposed in this paper. Firstly,a feature selection method based on Chi-square test (χ2) and Pearson correlation coeffects is developed to analyze the strength of fault correlation factors from multiple dimensions and the optimal set of fault features is obtained. Then,an optimal network reconfiguration model is established for the damaged ADNs considering DG integration,and consequently the heterogeneity of the line importance can be taken into account which provides a solid foundation for the classification of fault risks. Furthermore,an intelligent forecast model for ADN fault risk levels is established based on extreme gradient boostig (XGBoost) algorithm. Finally,the numerical tests on IEEE RBTS Bus6 distribution network demonstrate that the proposed approach achieves a predication accuracy 3.17% higher than back propagation (BP) neural network does. The proposed approach has good generalization capability,thus providing an important basis for the fault risk management in ADNs to effectively reduce the fault loss.

Entomological evidence provides entry points and clues for cases detection, in terms of estimation of the postmortem interval （PMI）, and place and cause of death. In recent years, the feasibility of entomological evidence in practice has been proved by theories and cases. It especially plays an important role in the investigation of cases with unnatural death, no monitoring, and highly corrupt cadaver. However, there are still some key issues to be further studied and standardized before the application of entomological evidence to forensic practice, to improve the effect of entomological evidence in forensic investigation and trial. This paper retrospectively reviews key studies of the application of entomological evidence in forensic science, mainly including discussion of forensic entomology inspection standard, identification studies of sarcosaprophagous insect species, collection of sarcosaprophagous insect growth and succession data under different environments and forensic entomotoxicology. With the rapid development of information technology and biotechnology, applying artificial intelligence and whole genome sequencing technology in forensic entomology has become a new research direction, which can improve the application value and range of entomological evidence in forensic science.

Objective To investigate the expression and clinical significance of S100A14 and LOXL2 in papillary thyroid carcinoma tissues. Methods Paraffin blocks from 90 cases of thyroid lesion were collected to make tissue microarray. The expression of S100A14 and LOXL2 in 30 cases of nodular goiter (NG), 30 cases of classic papillary thyroid carcinoma (CPTC) and 30 cases of high invasion papillary thyroid carcinoma were detected by immunohistochemistry. The correlation of S100A14 and LOXL2 expression with the clinicopathological characteristics of papillary thyroid carcinoma patients was investigated. Results S100A4 and LOXL2 were highly expressed in papillary thyroid carcinoma. There was a gradually increasing trend from the expression in NG group, CPTC group to high invasive group. The positive rate of S100A14 in papillary thyroid carcinoma with lymph node metastasis was higher than that without lymph node metastasis (P < 0.05). There was significant difference of S100A14 and LOXL2 expression between CPTC group and high invasion group (P < 0.05). S100A14 expression was positively correlated with LOXL2 expression (r=0.332). Conclusion The high expression of S100A14 and LOXL2 may be related to the malignant biological behavior and invasiveness of papillary thyroid carcinoma, and S100A14 may promote lymph node metastasis.

The instantaneous output power of a two-stage single-phase inverter pulsates at twice the output voltage frequency, generating second harmonic current(SHC) in the front-end dc-dc converter. The optimization of loop control of the front-end dc-dc converter is an effective and costless method for this problem. This study gives equivalent circuit model of the front-end dc-dc converter with single voltage loop control and voltage current dual-loop control first. Then, the propagation characteristic of the SHC is analyzed from the point of virtual impedance. On this basis,this study proposes a synthesis of different control strategies for SHC reduction and points out the limitations of them.In the view of this, this paper presents corresponding optimization methods. Finally, a 1 500-VA prototype model is built and simulated, and the simulation results are presented to verify the effectiveness of the analysis and the optimization.

Objective To investigate the expression of LncRNA LINC00857 in pancreatic cells and the effect of lncRNA LINC00857 down-regulation on proliferation, migration and apoptosis of pancreatic cancer PANC-1 cells and possible mechanism. Methods The lentiviral vector GV112 was constructed and infected PANC-1 cells to obtain experimental group, while the blank plasmid was transfected as a negative control group and the cells without intervention were taken as normal control group. CCK-8 assay, Transwell assay, scratch test, flow cytometry, Western blot were used to detect the effect of LINC00857 down-regulation on cell proliferation, migration, apoptosis, cell cycle and EMT-related proteins. Results LINC00857 expression in pancreatic cancer cells were significantly higher than that in normal pancreatic epithelial cells (P < 0.001). Knockdown of LINC00857 could significantly inhibit the proliferation and migration of PANC-1 cells (P < 0.0001); compared with the negative control group, the apoptosis rates of cells in the experimental groups were significantly increased (P < 0.05), the number of cells in G0/G1 phase increased (P < 0.01), the number of cells in S phase decreased (P < 0.05), and the cells were blocked in the G1 phase, E-cadherin expression was significantly up-regulated (P < 0.05) and N-cadherin and Vimentin expression were significantly down-regulated (N-cadherin: P < 0.01, Vimentin: P < 0.05). Conclusion LINC00857 can promote proliferation and migration of PANC-1 cells and inhibit its apoptosis by regulating G1/S phase transition and EMT signaling pathway.

By testing the parameters of graphite digestion instrument， the effects of graphite digestion method and microwave digestion method on the determination of total arsenic in rice were analyzed and compared.The results showed that the RSD of rice standard sample digested by graphite digestion method was 5.25%～6.86%， and the relative error between the test result and the standard value was 5.66%～7.55%. The RSD of rice standard sample digested by microwave digestion method was 8.67%～12.26%， and the relative error was 17.96%～21.7%. The accuracy and precision of graphite digestion method are higher than that of microwave digestion method.

Guided bone regeneration (GBR) barrier membranes are of great significance for the reconstruction of the health and function of different periodontal tissues. Biocompatibility, spatial maintenance, closure, controllability and biological activation are the main criteria that should be met by these membranes. Artificial barrier membrane biomaterials can be divided into synthetic polymer materials, natural polymer materials and metals. According to their degradation characteristics, these membranes can be divided into two categories, absorbable and nonabsorbable membranes. GBR used for horizontal bone increments can be used to treat various types of bone defects, including the treatment of bone fenestration and bone cracking. The use of a non-absorbable e-PTFE membrane or absorbable collagen membrane can achieve the expected effect. However, for incremental or vertical bone growth at the alveolar crest, the use of this membrane is very challenging and requires good strength to maintain the osteogenic space. This space can be enhanced with e-PTFE or d-PTFE membranes with stable morphology, or absorbable membranes can be covered with titanium plates or meshes to achieve vertical bone increments. Currently, bioactive membranes, digital 3D-printed titanium membranes and piezoelectric active biological membranes are research hotspots. In future research, the biological activation of these membranes will be further improved, which will promote the development of artificial membranes in the next stage.

Objective To investigate the expression of MYEOV in human pancreatic cancer cells and its effect on the proliferation and migration of pancreatic cancer SW1990 cells after knockdown. Methods The lentiviral vector GV248 was constructed and infected SW1990 cells to obtain two experimental groups (SW1990-sh1 and SW1990-sh2), while the blank plasmid was transfected as a negative control group and the untreated cells were taken as blank control group. MYEOV mRNA and protein expression were detected by qPCR and Western blot before and after transfection. The cells proliferation was determined by CCK-8 assay; the cell migration ability was analyzed by scratch test; The mRNA expression of SMADs in the TGF-β/SMAD signaling pathway was detected by qPCR. Results Compared with the pancreatic ductal epithelium cell line HPDE6, the mRNA expression of MYEOV in six pancreatic cancer cells were significantly increased (P < 0.01). However, low expression of MYEOV protein was detected only in PANC-1 and SW1990 cells. The mRNA and protein expression of MYEOV in the experimental groups were significantly downregulated. The proliferation and migration of SW1990 cells were lower than the control group (P < 0.001). MYEOV knockdown could reduce the mRNA expression of SMAD1, SMAD4, SMAD5 and SMAD9 in TGF-β pathway, while the expression of SMAD2, SMAD3 and SMAD7 mRNA were only down-regulated in the SW1990-sh2 group (P < 0.001). Conclusion MYEOV mRNA is overexpressed in pancreatic cancer cells. MYEOV could promote the proliferation and migration of pancreatic cancer cells via TGF-β/SMAD pathway.

Objective To investigate how oxaliplatin regulates the MAPK pathway and inhibits the proliferation of gastric cancer cells. Methods From NCBI, TargetScan, StarBase and miRBase databases were used to perform GO analysis and KEGG pathway enrichment in DAVID analysis, to find related miRNAs and predict their target genes. The proliferation, cell cycle, invasion and protein expression of SGC-7901 cells were analyzed by Real-time PCR, MTT, Hoechst33258, flow cytometry, scratch assay and Western blot, respectively. Results miR-7-5p expression was significantly downregulated in gastric cancer cells. RAF1 and miR-7-5p were co-targeted. The apoptosis of SGC-7901 cells was promoted, the percentage of G1 phase cells and the expression of caspase3 and caspase9 were increased, while the percentage of G2 phase cells and the ratio of Bcl-2/Bax protein were decreased in miR-7-5p mimics and oxaliplatin (OXA) groups(P < 0.05). Conclusion Both overexpression of miR-7-5p and oxaliplatin could promote the apoptosis of gastric cancer SGC-7901 cells, suggesting that oxaliplatin may promote the apoptosis of SGC-7901 cells and reduce the rates of invasion and migration by up-regulating miRNA-7-5p.

With the rapid development of the Internet, each platform often uses metadata to organize and manage system data resources. The system within the platform is closed so that metadata resources are dispersed and stored repeatedly, which increases the difficulty of metadata sharing and reduces the utilization of metadata. In order to solve this problem, this paper proposes a public metadata configuration management platform, which provides unified configuration and management of public metadata, provides configuration and shared metadata services by using Web Service technology, and improves data sharing efficiency by using Redis memory database. At present, a configuration management platform based on meteorological public metadata has been used in the meteorological big data platform, which not only improves the utilization of the platform common metadata, saves storage, but also improves the response speed of the service. This article abstracts the main functions of the configuration management platform, making the system design applicable to other similar metadata-based data sharing platforms.

Objective To investigate the mechanism of lncRNA MEG3 (MEG3) modulating the proliferation and metastasis of pancreatic cancer cells via regulating miR-543/PTEN axis. Methods qRT-PCR was applied to detect the expression of MEG3 in pancreatic cancer tissues and cell lines. The effect of MEG3 overexpression on the proliferation, invasion and epithelial mesenchymal transition (EMT) of PANC-1 cells were determined by CCK-8, Transwell assay and Western blot, respectively. The interaction between MEG3, miR-543 and PTEN were verified by dual-luciferase reporter gene assay. Furthermore, we investigated the effect of MEG3 on proliferation, invasion and EMT of PANC-1 cells through regulating miR-543/PTEN axis. Results MEG3 expression was down-regulated in pancreatic cancer tissues and cell lines. Meanwhile, the overexpression of MEG3 inhibited the proliferation, invasion and EMT of PANC-1 cells in vitro. MEG3 suppressed the proliferation, invasion and EMT of PANC-1 cells via decreasing the inhibitory effect of miR-543 on PTEN in vitro. Conclusion MEG3 inhibits the proliferation, invasion and EMT of PANC-1 cells by regulating miR-543/PTEN axis, which may provide potential molecular targets for early diagnosis and treatment of pancreatic cancer.

Homogenization heat treatment on the as-cast Mg-13Gd-3.5Y-2Zn-0.5Zr magnesium alloy was carried out with the temperature range of 505-525℃ and the time range of 4-24h. Optical microscope(OM), scanning electron microscope(SEM), X-ray diffractometer(XRD) and universal material experiment machine were used to analysize the changes of microstructure evolution and mechanical property before and after homogenization. The results show that the reticular eutectic compounds in initial grain structure are transformed into discontinuous bulk-shaped LPSO (long-period stacking ordered) phases at the grain boundary and the discrete distribution of the square-shaped rich rare-earth phases dissolved. The mechanical properties test results show that the ultimate tensile strength and the elongation of the cast magnesium alloy are 172.9MPa and 1.8%, respectively. The mechanical properties of the alloy are improved after homogenization, the ultimate tensile strength of room temperature is 212.3MPa and the elongation is 3.1% under the homogenization of 515℃/16h. At the same time, the ultimate tensile strength of high temperature at 200℃ reaches the highest 237.2MPa and the elongation is 9.7%. The fracture microstructure of as-cast alloy indicates that the fracture is cleavage brittle fracture dominated by tearing edges and cleavage steps, small and shallow dimples occur in the alloy after homogenization treatment, but is still quasi-cleavage fracture dominated by cleavege steps, and limited plasticity is improved. Meanwhile, LPSO phase can be the crack initiation source.

Objective To explore the association of single nucleotide polymorphism (SNP) at rs6967330 of cadherin-associated family member 3 (CDHR3) gene with acute respiratory tract infection caused by human rhinoviruses (HRVs) in children. Methods From January, 2012 to November, 2014, we collected specimens of nasopharyngeal aspirates from 200 children with acute lower respiratory tract infection caused by HRVs. CDHR3 gene fragment was amplified by PCR from the samples and sequenced for rs6967330 genotyping. The VP4 gene of HRV was amplified with PCR for subsequent sequencing, and real-time fluorescent quantitative PCR was used to estimate the virus load. The correlation between rs6967330 genotypes and the clinical features of the children was analyzed. Results Of the 200 children with acute lower respiratory tract infection caused by HRVs, 30 (15.0%) had AA/AG genotype and 170 (85.0%) had GG genotype of rs6967330. The children with AA/AG genotype were more likely to have wheezing symptoms than those with GG genotype (86.7% vs 57.1%, P=0.002), and were also more susceptible to severe pneumonia (30.0% vs 11.2%, P=0.018); the first-degree relatives of the children with AA/AG genotype were also more likely to have a history of atopic diseases(16.7% vs 5.88%, P=0.039). Logistic regression analysis revealed that rs6967330 A was a risk factor for wheezing in children with HRV infection (OR: 4.585, 95% CI: 1.446~14.541, P=0.010) and also for progression into severe cases (OR: 3.379, 95% CI: 1.009~11.315, P=0.048). No significant differences in HRV subtypes or viral loads were found between the children with different genotypes of rs6967330. Conclusion The SNP of CDHR3 gene at rs6967330 may be associated with severe cases and wheezing after lower respiratory tract infection with HRV in children.

Objective To detect the expression and methylation status of long non-coding RNA ZNF667-AS1 in esophageal squamous cell carcinoma(ESCC) tissues. Methods qPCR and MSP Methods were respectively applied to detect the expression and methylation status of ZNF667-AS1 in esophageal cancer cell lines (Kyse170, Eca109, TE1, TE13), ESCC and corresponding noncancerous tissues before or after DNA methyltransferase inhibitor 5-Aza-dC treatment. Results After treated with 5-Aza-dC, ZNF667-AS1 performed higher expression in the four esophageal cancer cell lines, and the methylation status of ZNF667-AS1 were decreased. The expression of ZNF667-AS1 in ESCC tissues was significantly lower than that in the corresponding normal tissues, and the methylation frequency of ZNF667-AS1 distal and exon1 regions CpG islands were all significantly different(P < 0.05). The methylation frequency of ZNF667-AS1 proximal promoter region CpG island in ESCC tissues was significantly higher than that in the corresponding normal tissues, and closely correlated with pathological differentiation and TNM stages; the methylation frequency in the low ZNF667-AS1 expression group was significantly higher than that in the high ZNF667-AS1 expression group. Conclusion The low expression of ZNF667-AS1 is closely related to the occurrence and development of ESCC, and its proximal promoter region methylation may be one of the mechanisms of ZNF667-AS1 silence.