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To investigate the effect of the interaction between protocatechuic acid (PCA) and gelatin on the functional properties and stability of gelatin-PCA complexes. Gelatin-PCA complexes were prepared by non-covalent and covalent binding. Afterward, the interaction between PCA and gelatin was analyzed using UV-Vis spectroscopy, fluorescence spectroscopy, and Fourier transform infrared spectroscopy. Meanwhile, an analysis was also performed on the antioxidant activity, antibacterial activity, and stability of the complexes. The findings showed a higher PCA binding rate for the covalent complexes than the non-covalent complexes. Additionally, the interaction between PCA and gelatin resulted in the fluorescence quenching of gelatin, with a higher level of fluorescence quenching observed for the covalent binding. Moreover, in addition to excellent DPPH and ABTS+ radical scavenging abilities. Compared with GPCA-2，the non-covalent complex GPCA-1 showed more significant (P

Objective To establish reference intervals for serum soluble transferrin receptor (sTfR) and sTfR/log serum ferritin index (sTfR/lgSF) in apparently healthy adults in the Wuhan area, so as to provide reference for clinical diagnosis and treatment of iron deficiency and iron-deficiency anemia. Methods A total of 273 individuals from the Wuhan Aisa General Hospital, including health examination participants and blood donors, were selected to measure sTfR, other iron metabolism indicators and high sensitivity C-reactive protein(hsCRP). The sTfR/lgSF values were calculated and reference intervals for sTfR and sTfR /lgSF were established using the percentile method (P2.5 to P97.5). Spearman correlation analysis was used to evaluate the relationships between sTfR, sTfR/lgSF, and other iron metabolism indicators, as well as hsCRP. Results The sTfR levels (M, mg/L) between males and females (1.01 vs 1.07) were not statistically significant (P>0.05), but the sTfR/lgSF levels if males were significantly lower than those in females (0.45 vs 0.62)(P0.05). The reference interval for STfR was (0.72-1.68) mg/L, the sTfR/lgSF reference interval was (0.31-0.88) for males, and (0.37-1.19) for females. Correlation analysis showed no correlation between sTfR, sTfR/lgSF and hsCRP (r=0.043, P>0.05; r=-0.064, P>0.05), while serum ferritin(SF), serum iron(SI), transferrin saturation(TSAT)were correlated with hsCRP (r=0.128, P0.05), while sTfR/lgSF was significantly correlated with and SF (r=-0.685, P

In order to investigate the effect of ABP (Agaricus bisporus powder) on the physicochemical properties of yogurt, ABP with different mass fractions (0.0%, 1.5%, 3.0%, 4.5%, 6.0% and 7.5%) was added to yogurt while using yogurt as the raw material, and the rheological and textural properties of yogurt were investigated at different ABP additions. The findings demonstrated that with the addition of ABP at 1.5%, the dynamic viscoelasticity, apparent viscosity, consistency, hardness, and cohesiveness of yogurt were decreased compared with the control group, while the dynamic viscoelasticity, apparent viscosity, consistency, hardness and cohesiveness of yogurt increased with a higher addition of ABP at over 1.5%. Moreover, the scanning electron microscopy results suggested that yogurt with ABP addition at 0.0% and 1.5% showed casein fibrillar network structure with more voids, and with the increase of ABP addition, the voids in the fibrous network were gradually filled by ABP particles, thus forming a dense and continuous spatial structure, which ultimately enhanced the hardness, consistency, and stability of the yogurt. According to the comprehensive analysis, the addition of ABP at 1.5%~3.0% will result in better rheological and textural properties of yogurt. In conclusion, these findings could provide theoretical and data support for the research and development of Agaricus bisporus-flavored dairy products.

Objective When urban rail transit trains operate on elevated tracks, crosswinds have a significant impact on the stability and safety of vehicle operation. It is essential to conduct a more in-depth study on the dynamics performance of metro vehicles under crosswind action. Method Three classical crosswind load models (classical constant steady-state wind load model, transient Chinese hat wind load model, and non-steady-state stochastic wind load model) in MATLAB software are employed to simulate dynamic wind fields. In SIMPACK software, a detailed rigid-body dynamics model of a single-car formation train is established. The three wind load models are applied as external excitations to the vehicle. Comparative analysis is conducted on the influence of different wind load models, wind speeds, and vehicle speeds on the operational stability of metro vehicles when operating on straight tracks. From a safety design perspective, the non-steady-state stochastic wind load model is selected to analyze the stability and safety of the vehicle during curve negotiation, exploring the safety domain of vehicle speeds under different wind speeds. Result & Conclusion Under crosswind action, both metro vehicle speed and wind speed significantly influence vehicle operational stability and safety. During train high-speed running, wind speed has a more pronounced impact on the lateral vibration of the vehicle. Among the three crosswind load models, the non-steady-state stochastic wind load model has the most significant impact on vehicle stability. Based on the analysis of vehicle stability and safety, speed limits for vehicles are determined for different route geometries (straight or curve tracks) and wind speeds.

This is an essay in the field of environmental engineering. The experiment adopts the impregnation method, and uses FeCl3 as the modifier to modify the activated carbon to study its desulfurization ability. The effects of modifier concentration, roasting temperature, and reaction temperature on the desulfurization performance of modified activated carbon were experimentally studied. Studies have shown that as the concentration of the modified solution increases, the Fe2O3 attached to the surface of the activated carbon increases, the specific surface area and total pore volume of the modified activated carbon decrease, and the average pore size increases; as the roasting temperature increases, the amount of Fe2O3 attached to the surface of the activated carbon continues. When the roasting temperature exceeds 300 ℃, the pore structure of the activated carbon surface will be sintered, which will reduce the desulfurization performance of the modified activated carbon; as the reaction temperature increases, the adsorption performance of FeCl3/AC-0.15 first increases and then decreases. When the concentration of FeCl3 modified solution is 0.15 mol/L, the calcination temperature is 300 ℃, and the reaction temperature is 60 ℃, the desulfurization efficiency of modified activated carbon is the highest.

Objective To investigate asymptomatic infection of hepatitis B virus(HBV) among hepatitis B vaccinated donors in Shenzhen, and analyze its serological and molecular characteristics. Methods The HBsAg ELISA positive blood samples of blood donors born after 1992 were collected. HBsAg, anti-HBs, HBeAg, anti-HBe and anti-HBc were further detected by Roche electrochemiluminescence immunoassay (ECL). BCP/PC and S regions were amplified by Nested-PCRs, HBV DNA quantification were adopted by qPCR simultaneously, and the sequences were also analyzed. Results A total of 46 632 blood samples of donors(31 612 males and 15 020 females) from December 2020 to January 2022 collected, and 99 samples with HBsAg ELISA positive were screened out. After tested by ECL, Nested-PCRs, and real-time fluorescence PCR, 61 were confirmed HBsAg positive, with the positive rate at 0.13% (61/46 632), including 49 males (0.16%, 49/31 612) and 12 females (0.08%, 12/15 020). The HBsAg positive rate of males was higher than that of females (P

Objective To analyze the results of different methods for reactive samples screened by the enzyme linked immunosorbent assay (ELISA) against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in blood donors. Methods From March to April 2020, a total of 8 632 blood samples in Shenzhen were screened for SARS-CoV-2 total antibodies (TAb, including IgG, IgM, IgA) in plasma using ELISA(PC group), the antibody reactivity samples and their follow up plasma samples (FC group), and samples of disease control group(DC group) from January to April 2020 were detected using the following methods: 1) ELISA method for detecting IgG, IgM, and (or without detection) TAb; 2) pseudovirus neutralizing antibody test(pVNT); 3) western blot (WB) of SARS-CoV-2 antibody. The negative control group(NC group) from February to April 2020 performed ELISA and WB testing. Results Among the 34 total antibody positive samples, 2 were positive for pVNT test, and the total antibody, IgG and WB in the initial screening and tracking testing were positive. Thereafter, it was determined to be confirmed positive. The other 2 cases were positive for pVNT test, while the samples with positive WB results were in the follow-up stage. The TAb, IgG, and pVNT results did not conform to the dynamic evolution of antibodies, and cannot be determined as confirmed positive. Conclusion The infection status of antibody reactivity samples screened by SARS-CoV-2 ELISA can be judged by the logic of pVNT, WB and the dynamic change of antibody.

Objective To establish a magnetic bead enrichment strategy for the detection of human immunodeficiency virus deoxyribonucleic acid (HIV DNA) in peripheral blood, and to verify the improvement of the sensitivity of this method for the detection of HIV DNA in HIV infected patients after early antiretrovital treatment (ART). Methods Peripheral whole blood was collected at 4 timepoints in one ART HIV window period (WP) patient. Peripheral blood mononuclear cells (PBMCs) were isolated on a Ficoll gradient. CD4+ T lymphocytes were enriched from total PBMCs by negative sorting. HIV DNA concentration in magnetic beads enriched group and whole blood group was detected by HIV DNA detection kit. Results CD4+ T cells were isolated by magnetic beads and identified by FCM for purity at (96.4 ± 2.6)%. The viability was (95.9 ± 2.9)%, as demonstrated by trypan blue staining. The person on continued ART treatment in this study had significantly greater reduction in HIV viral load and undetectable HIV plasma RNA at follow up timepoint 4. No HIV DNA was detected in the whole blood group at all 4 timepoints. The quantitative results of HIV DNA in the CD4+ T lymphocyte group of the magnetic bead enrichment group were 73.4, 429.3, 137.1, 449.9 copies/106 CD4+ T cell′s respectively. Conclusion The magnetic bead enrichment method can be more sensitive in detecting the limit low copy HIV DNA in blood samples, and provide early confirmatory data for HIV WP infection and breakthrough infection after ART treatment.

The risk of lung injury induced by microorganism infection or other physical/chemical insults is high due to the nature that the lung is constantly exposed to microbiota through inhalation. The lung was previously thought to be a highly quiescent tissue, which has limited reparative capacity. However, recent studies demonstrate that lung regeneration could be achieved as the tissues of the lung have facultative progenitor cell populations that can be activated to proliferate and differentiate in response to various injuries. There are several types of macrophages within the lung tissue, which play a key role in different phases of lung injury and are closely associated with lung regeneration after injury. Fine-tuning of the differentiation monocytes /macrophages could serve as an effective therapeutic approach to alleviate lung injury and promote lung regeneration.

Exploitation of mineral resources and construction of traffic facilities have led to the destruction of a large number of mountains, resulting in the formation of high and steep rock slopes, which have caused serious geological safety hazards and ecological function degradation. The ecological restoration of high and steep rock slopes is imperative. The key of slope ecological restoration is vegetation reconstruction. At present, there are still many problems such as lack of restoration theory and unclear reconstruction conditions. In order to clarify the relationship between the temperature and humidity fields and the plant growth in the rock slope, this study established a test site near Xiaodongshan in the city of Zhangqiu in Shandong Province. Multi-period monitoring experiments on the temperature and humidity field in the rock slope were carried out, the distribution characteristics of the temperature and humidity field in the rock slope are examined, and their ecological significance is analyzed in detail. The results show that (1) the atmospheric temperature causes the difference of the temperature variation in the monitoring hole in different seasons. In winter, the heat in the unsaturated zone of fractured rock mass is transferred from deep layer to surface layer, but in spring and summer it is opposite, The depth of the variable temperature zone ranges from 0 − 467 cm, and the depth of the constant temperature zone is greater than 467 cm. (2) In winter, the relative humidity in the slope first increases and then decreases with the increasing depth. In spring and summer, the relative humidity in the slope increases gradually with the increasing depth. (3) The water vapor saturated zone appears near the depth of 20 cm in winter, and begins to appear at the depth of 20 − 40 cm in summer and extends deeper. (4) The temperature and humidity in the rock mass are suitable for the growth of plants, and the plants are easier to survive if they are planted near the depth of 20 cm in spring. The results are of has important theoretical significance and application value for guiding the ecological restoration of rock slopes.

Under the background of global carbon neutrality, soil carbon accumulation and transformation become a hot topic in academic circles. In the process of land reclamation and restoration, the change and its mechanism of soil carbon pool in coal mining subsidence area under the influence of groundwater are complicated. The study of carbon dynamics in reclaimed soil is an important basis for a scientific restoration of reclaimed soil ecosystem. In this study, the age chronosequense approach and the location-monitoring method were used to study the change of soil carbon pool after the reclamation of coal mining subsidence area in the area with higher level of underground water. Soil samples from the study areas were collected twice, respectively in 2012 and 2020. The content of soil total carbon (CTC, total organic carbon (CTOC), total inorganic carbon (CTIC) were measured, and soil carbon storage of TC pool (STC), TOC pool (STOC), TIC pool (STIC) and rates of carbon sequestration were calculated. The soil properties (i.e., soil bulk density, compaction, porosity and soil structure, etc.) associated with the change of soil carbon were also analyzed simultaneously. The results show that: ① the STC, STOC and STIC of soil in non-subsidence area (CK) were 268.34, 81.63, 186.70 t/hm2 respectively. Land subsidence caused the decrease of STC and STIC, but with no significant change of STOC. ② A quadratic equation curve was shown in the change of soil carbon density and carbon storage in the restoration process of reclaimed soil, the CTC and CTIC of 0−20 cm layer reaching the maximum in 14−18 years after reclamation, and exceeding CK. The STOC could exceed CK in 13−14 years of reclamation. ③ There was no significant difference in carbon density and carbon pool between layers in 0−40 cm of newly reclaimed soil. In the process of restoration after reclamation, the largest increase of carbon density and carbon storage was found in the surface layer (0−10 cm), and there was no regular variation of soil CTIC in the profile for study. ④ The soil CTOC, STOC and CTC were positively correlated with soil porosity, soil clay, fractal dimension (D) and content of aggregate larger than 3.2 mm, but negatively correlated with bulk density, compactness, pH and aggregate content of 2−0.50 mm. There was great potential for carbon sequestration in the reclaimed soil. Soil moisture affected the carbon fixation and transformation of reclaimed soil by influencing soil aeration, aggregate formation and particle change in the area with higher level of underground water. The cultivation could improve soil structure and ventilation condition, promote the growth of plants and soil microorganisms, increase the source of soil organic carbon, and promote the accumulation of reclaimed soil organic carbon by ploughing, crushing, applying organic fertilizer and water level adjustment.

Kinship testing is widely needed in forensic science practice. This paper reviews the definitions of common concepts, and summarizes the basic principles, advantages and disadvantages, and application scope of kinship analysis methods, including identity by state （IBS） method， likelihood ratio （LR） method， method of moment （MoM）， and identity by descent （IBD） segment method. This paper also discusses the research hotspots of challenging kinship testing, complex kinship testing, forensic genetic genealogy analysis, and non-human biological samples.

ObjectiveTo establish a real-time quantitative polymerase chain reaction （PCR） method for rapid and accurate identification of Cronobacter spp. in food samples and artificially contaminated samples.MethodsPrimers and probes were designed based on the conserved region of gyrB of Cronobacter spp. DNA. The method was verified using a specificity test， absolute sensitivity test， relative sensitivity test， and anti-interference test. Detection sensitivity was determined using artificially contaminated samples.ResultsThe method established in this study could specifically amplify seven kinds of Cronobacter spp.， but not the other Enterobacter species closely related to it and other pathogens common in food， suggesting that this method has good anti-interference ability. The absolute sensitivity was 1-10 pg and the relative sensitivity was 103 CFU/mL using Cronobacter sakazakii. It had good anti-interference ability at the genome and culture level. The sensitivity of artificially contaminated samples could reach 100 CFU/mL after incubation at 36 ℃ for 24 h.ConclusionThe real-time PCR method developed in this study is rapid， specific， sensitive， and stable for the detection of Cronobacter spp. in infant formula food samples and can provide technical reference for the detection of Cronobacter spp. in infant formula food.

Underground personnel behavior recognition is an important measure to ensure safe production in coal mines. The existing research on behavior recognition of underground personnel lacks research and analysis on the perception mechanism, and the feature extraction method is simple. In order to solve the above problems, a behavior recognition method for underground personnel based on fusion networks is proposed. The method mainly includes three parts: data preprocessing, feature construction, and recognition network construction. Data preprocessing: the collected channel status information (CSI) data is processed through CSI quotient models, subcarrier denoising, and discrete wavelet denoising to reduce the impact of environmental noise and equipment noise. Feature construction: the processed data is transformed into images using the Gramian angular summation/difference fields (GASF/GADF) to preserve the spatial and temporal features of the data. Recognition network construction: according to the features of personnel actions, a fusion network composed of a gate recurrent unit (GRU) based encoding and decoding network and a multiscale convolutional neural network (CNN) is proposed. GRU is used to preserve the correlation between pre and post data. The weight allocation strategy of the attention mechanism is used to effectively extract key features to improve the accuracy of behavior recognition. The experimental results show that the average recognition accuracy of this method for eight movements, namely walking, taking off a hat, throwing things, sitting, smoking, waving, running, and sleeping, is 97.37%. The recognition accuracy for sleeping and sitting is the highest, and the most prone to misjudgment are walking and running. Using accuracy, precision, recall, and F1 score as evaluation indicators, it is concluded that the performance of the fusion network is superior to CNN and GRU. The accuracy of personnel behavior recognition is higher than the HAR system, WiWave system and Wi-Sense system. The average recognition accuracy of walking and taking off a hat at normal speed is 95.6%, which is higher than 93.6% for fast motion and 92.7% for slow motion. When the distance between transceiver devices is 2 m and 2.5 m, the recognition accuracy is higher.

Objective In an effort to prevent transfusion-transmitted hepatitis B infection, universal HBsAg screening, HBsAg+ MP nucleic acid test(NAT) for HBV and HBsAg + individual(ID) NAT were analyzed for cost-effectiveness. Methods On the basis of screening data and the documented parameter, the number of window period infections, chronic infections and occult infections was constructed, and cost-benefit analysis was conducted. Results Of 132 208 donations, the yield rate of ID NAT for HBsAg-/DNA+ (0.11%) was significantly higher than HBsAg+ MP NAT(0.058%). Furthermore, the predicted preventing transfusion transmitted HBV cases by ID NAT is 1.25 times as that by MP-6 NAT, so did the benefits. The cost-benefit of the three screening models were 1∶63.6、1∶28.6 and 1∶53.4. Conclusion Universal HBsAg in combination with ID HBV NAT screening was the most effective among all screening strategy. It is necessary to applied HBsAg and ID HBV NAT screening for the safety of blood transfusion.

To analyze the potential causes of peritoneal dialysis-associated refractory peritonitis and summarize related nursing measures. The clinical treatment and nursing process of two cases of peritoneal dialysis-associated refractory peritonitis were retrospectively analyzed. The operating procedure of peritoneal dialysis was not strictly followed in these two patients with slim body type and hypoproteinemia, and treatment was delayed due to insufficient awareness about peritoneal dialysis -associated peritonitis. Anti-infective treatment was administrated for intra-abdominal and peripheral infection, and treatment was switched to hemodialysis as no obvious improvement of bacteriological examination results. The treatment environment, operation procedure, intestinal factor and awareness of disease were main factors influencing the peritoneal dialysis -associated refractory peritonitis, and efforts should be made to enhance the self-management skills of peritoneal dialysis patients. (探讨腹膜透析相关性难治性腹膜炎潜在原因及拔管后相应的护理措施。回顾性分析科室2例难治性腹膜炎患者的临床诊疗及护理经过, 总结疾病临床特征及护理经验。2例患者均体型偏瘦, 且存在低蛋白血症, 日常未严格按操作规程进行, 同时未引起对腹膜透析相关性腹膜炎的重视度, 导致延时就诊。入院后给予腹腔及外周抗感染治疗后10～20 d, 腹腔细菌学检查无明显改善, 给予拔管, 改行血液透析。环境、操作、肠道原因、营养状态、对腹膜炎的认识及重视度是导致难治性腹膜炎发生的原因, 应加强对腹膜透析患者的自我管理。)

Intelligent fluid supply technology in working face is one of the core technologies of intelligent mining. In order to solve the requirements of high data transmission rate, high transmission stability and high data processing ability in the intelligent work face, a centralized-distributed emulsion station control system based on the 100 megabit industrial Ethernet was developed, fault prediction and health management technology and intelligent on-demand liquid supply control technology were studied, which improves intelligent control function, and the development demand of the working media quality assurance technology of the work face hydraulic system was analyzed, and key technologies such as underground integrated reverse osmosis (RO) water treatment technology, high-efficiency multi-stage filtration technology, fully automated emulsion automatic ratio and concentration correction technology and other key technologies were innovated. In order to overcome the key technical problems such as high-strength corrosion-resistant materials, reliability technology of the suction and discharge valves under pure water medium and pure water resistivity assurance technology, the development of intelligent fluid supply system under pure water condition has promoted the development of green mining technology. The core technical problems such as unattended intelligent safety control technology, timely and rapid liquid supply and return relay technology, integrated multi-parameter safety monitoring of long-distance supply pipeline technology with integrated multi parameter monitoring have been overcome, and the problems of remote safe and efficient liquid supply in mines with complex geological conditions have been solved.. This achievement has been popularized and applied in the fully mechanized mining and fully mechanized caving working faces of thin and medium thick coal seams in Shendong Mining Area of National Energy Group, Shanxi Mining Area of China Coal Energy Group, and Yankuang Mining Area of Shandong Energy Group, meeting the demand for liquid supply technology and equipment in the development of intelligent mining at this stage.

Objective To investigated when and how to retraining in peritoneal dialysis(PD) patients and its role in decreasing the incidence of peritonitis according to analysis of the characteristics of peritonitis in the PD center. Methods In a retrospective study, 2 years of data from 2019 before continuous quality improvement(CQI) and 2020 after CQI in our PD center were analyzed. According to analyzing the characteristics of peritonitis before CQI, when and how to retrain PD patients were put forward. The incidence and the characteristics of peritonitis were calculated and analyzed before and after CQI. Results There were 27 episodes of peritonitis in 22 out of total 99 PD patients in 2019. Totally 17 cases with peritonitis occurred for the first time and 11 of them (64. 71%) occurred in their first 6 months after PD initiate. Culture-positive was in 12 episodes (among them, 7 episodes of Coagulase-negative Staphylococcus or Staphylococcus Aureus). The whole year peritonitis rate was 1 episode every 35. 2 months in 2019 before CQI. The first retraining was taken in the third month after PD initiate and redirecting their behavior was made. There were 11 episodes of peritonitis in 10 patients out of 101 patients after CQI in 2020. There were 9 episodes of peritonitis occurred for the first time and 2 of them (22. 22%) occurred in their first 6 months after PD initiate. The rate of culture-positive peritonitis was increased to 63. 64%. In the 7 culture-positive episodes, 3 were Staphylococcus Aureus and 4 were Escherichia coli. The whole year peritonitis rate decreased to 1 episode every 108. 5 months in 2020 after CQI. Conclusion First retraining in the third month after PD initiate and redirecting their behavior to reduce the risk of peritonitis was an effective program in PD center. (目的 分析医院腹膜透析中心腹膜透析相关性腹膜炎发生原因、特点, 探讨再培训的时机、内容及实施效果。方法 选择河北医科大学第三医院腹膜透析中心因终末期肾脏病而行腹膜透析治疗并能规律随访的患者。分析2019年本中心腹膜炎发生率及特点, 根据持续质量改进的原则, 提出再培训的时机、内容、护理流程, 并观察实施效果, 与2020年腹膜透析患者腹膜炎发生率及特点进行比较。结果 2019年99例腹膜透析患者中22例患者发生27例次腹膜炎。全年腹膜炎发生率为1/35. 2个病人月。其中有17例患者首次发生, 11(64. 71%)例发生于腹膜透析置管后的6个月内。12例次培养阳性, 其中凝固酶阴性葡萄球菌及金黄色葡萄球菌感染7例次。将第一次再培训时机设为开始腹膜透析后的第3个月, 主要内容为规范操作。实施再培训后, 2020年本中心101例腹膜透析患者共10例患者发生11例次腹膜炎, 全年腹膜炎发生率为1/108. 5个病人月, 9例首次发生腹膜炎的患者中2(22. 22%)例发生于置管术后6个月内。7(63. 64%)例次培养阳性, 其中金黄色葡萄球菌感染3例次, 大肠杆菌4例次。结论 开展再培训计划, 可使医院腹膜透析中心的腹膜透析相关性腹膜炎的发生率明显减少。)

Powdered infant formula (PIF) is the main source of Cronobacter spp. contamination, among which the addition of raw and auxiliary materials and the processing environment are the most important routes of transmission. In order to effectively prevent and control Cronobacter spp. to ensure the safety of dairy products, eight strains of Cronobacter spp. isolated from PIF and its processing environment were used as the research objects. Their heat resistance, acid and alkali resistance, dryness resistance and permeability resistance were studied, and the differences of environmental resistance of different strains were analyzed. The results showed that Cronobacter spp. had different heat resistance. The strains isolated from the end products had relatively stronger heat resistance. Cronobacter spp. generally had the characteristics of acid resistance but not alkali resistance. Under the acidic conditions at pH1.5, all strains could still survive being treated for 30 s. Cronobacter spp. had strong resistance to desiccation. The bacterial count of all strains decreased from an initial concentration of 108 CFU/mL to 105 CFU/mL over the course of the 7-month test at room temperature with a relative air humidity of 20.7%. The slow decreasing trend indicated that Cronobacter spp. had a strong ability to resist dry environment. Cronobacter spp. had a strong resistance to osmosis. We found that all strains grew slowly when the concentration of NaCl was over 8% and the tolerance of Cronobacter spp. to sorbitol was stronger than that to NaCl under isotonic conditions.

Objective To explore the predictive value of the expression of CD44v6 and EGFR on the efficacy of neoadjuvant chemotherapy (NACT) in stageⅡ-Ⅲ cervical cancer. Methods A total of 53 patients with stageⅡ-Ⅲ cervical cancer diagnosed by pathology were selected. All patients received two cycles of paclitaxel+platinum NACT. The pathological tissue samples of cervical tumors before NACT treatment were collected. The expression of CD44v6 and EGFR were detected by the immunohistochemical SP method, and we analyzed their predictive value of NACT in stageⅡ-Ⅲ cervical cancer. Results Among the 53 patients, 38 were in the NACT effective group (CR+PR), and 15 were in the NACT ineffective group (SD+PD). The expression of CD44v6 in the ineffective group was significantly higher than that in the effective group (P < 0.05). The expression of CD44v6 was significantly different in patients with CR, PR, and SD (P < 0.05). The AUC of CD44v6 to NACT effect on stage Ⅱ-Ⅲ cervical cancer was 0.74 (P < 0.05). The patients in the high expression group of CD44v6 had worse efficacy in NACT than those in the low expression group of CD44v6 (P < 0.05). Pearson test showed that CD44v6 and EGFR expression were correlated (R=0.34, P < 0.05). Conclusion High expression of CD44v6 may reduce the efficacy of NACT in stageⅡ-Ⅲ cervical cancer, suggesting that the expression of CD44v6 has a certain predictive value and clinical significance in the efficacy of paclitaxel+platinum NACT on cervical cancer. Moreover, CD44v6 is positively correlated with EGFR expression.

BackgroundEsophageal cancer is a common gastrointestinal tumor with a high incidence in China. Some studies suggest that intestinal flora is significantly related to the occurrence and development of tumors and other diseases. Traditional 16S rDNA sequencing technology only provides taxonomic resolution at genus level. ObjectiveBased on PacBio single molecule real time (SMRT) sequencing technology to identify characteristic microbial biomarkers associated with esophageal cancer at the species level. MethodsA total of 120 newly diagnosed cases of esophageal cancer were recruited and 60 healthy patients with matched sex and age were recruited as the control group. Fresh stool samples were collected from all subjects. Full-length 16S rDNA sequencing was performed on samples from 4 patients with esophageal cancer and 1:1 matched healthy controls using the third-generation sequencing PacBio SMRT technology, and the structural differences of intestinal flora were analyzed based on the sequencing results. Function prediction was performed by PICRUSt software. Large population samples were validated by screening different gut microbes by linear discriminant analysis and linear discriminant analysis effect size to identify esophageal cancer-associated gut microbes. ResultsBased on sequencing samples, the results of α diversity analysis showed that the Ace, Chao1, Simpson Diversity, and Shannon Wiener indices of the esophageal cancer group were higher than those of the healthy control group (P

Objective To analyze the cost-effectiveness of ELISA grey area strategy through establishing the health economics model. Methods The serological grey area strategy evaluation model was composed of screening strategy subdecision tree, pathogen infection subdecision tree and pathogen detection subdecision tree. The key parameters in the model were obtained from literatures and research data. The cost-effectiveness of setting ELISA grey area strategy was compared by software, and multifactor sensitivity analysis was conducted. Results After setting the ELISA grey area, extra samples(5.86 cases/100 000) with serological false negativity could be detected, including HBV samples at 4.93/100 000, HCV samples at 0.27/100 000, HIV samples at 0/100 000, syphilis samples at 0.66/100 000. To yield an additional seropositive sample out of every 100 000 blood donors, blood center will afford extra 1 million yuan about. Conclusion Through this study, a cost-effectiveness evaluation model of serological detection strategy was established. Although the ELISA grey area setting can yield a small number of seropositive samples, the cost is much higher than the current affordability.

By integrating surface geology, seismic data, resistivity sections, and drilling data, the structural deformation characteristics of the frontier fault of thrust nappes were delineated in detail. The frontier fault of thrust nappes in northwest Scihuan Basin is a buried thrust fault with partial exposure in the Xiangshuichang—Jiangyou area, forming fault propagation folds in the hanging-wall and without presenting large-scale basin-ward displacement along the gypsum-salt layer of the Triassic Jialingjiang Formation to the Triassic Leikoupo Formation. The southwestern portion of the frontier fault of thrust nappes (southwest of Houba) forms fault bend folds with multiple ramps and flats, giving rise to the Zhongba anticline due to hanging-wall slip along the upper flat of the Jialingjiang Formation. In contrast, the northeastern portion of the frontier fault of thrust nappes (northeast of Houba) presents upward steepening geometry, leading to surface exposure of Cambrian in its hanging-wall. With the frontier fault of thrust nappes as the boundary between the Longmenshan Mountain and the Sichuan Basin, the imbricated structural belt in the hanging-wall thrusted strongly in the Indosinian orogeny and was reactivated in the Himalayan orogeny, while the piedmont buried structural belt in the footwall was formed in the Himalayan orogeny. In the footwall of the frontier fault of thrust nappes, the piedmont buried structural belt has good configuration of source rocks, reservoir rocks and cap rocks, presenting good potential to form large gas reservoirs. In comparison, the hanging-wall of the frontier fault of thrust nappes north of Chonghua has poor condition of oil/gas preservation due to the surface exposure of Triassic and deeper strata, while the fault blocks in the hanging-wall from Chonghua to Wudu, with Jurassic cover and thicker gypsum-salt layer of the Jialingjiang formation, has relative better oil/gas preservation conditions and thus potential of oil/gas accumulation. The frontier fault of thrust nappes is not only the boundary between the Longmenshan Mountain and the Sichuan Basin, but also the boundary of the oil/gas accumulation system in northwestern Sichuan Basin.

Objective To establish a routine screening method for unexpected antibodies of blood donors, analyze the results of centralized screening for unexpected antibody of blood donors in the blood center, and compare the cost of centralized and decentralized screening modes. Methods A total of 35 591 blood donors were screened for unexpected antibodies from March 31, 2021 to July 31, 2021, using microcolumn gel method. Unexpected antibody screening reactive samples were further confirmed by the Transfusion Research Institute of Shenzhen Blood Center, and the demographic characteristics were further determined through the analysis of unexpected antibody positive population. The direct cost and indirect cost of centralized and decentralized unexpected antibody screening mode were compared. Results Forty unexpected antibody positive samples were confirmed in Shenzhen, with the positive rate at 0.11%(40/35 591), among which MNS, Rh and Lewis system accounted for 35% (14/40), 32.5% (13/40) and 17.5% (7/40), respectively. Males and females accounted for 45% (18/40) and 55% (22/40), respectively (P0.05). Unexpected antibody screening in a centralized way saved about 1.16 million yuan per year. Conclusion It is necessary to carry out unexpected antibody screening for all blood donors, and centralized screening is more economical than decentralized screening.

Xanthan gum (XG) and soybean protein isolate (SPI) were used as raw materials to prepare XG-SPI mixed gel by ultrasonic pretreatment and glucolactone induction. Rheological tests and scanning electron microscopy were used to study the effects of different concentrations of XG on viscoelasticity, self-healing ability, thermal stability and microstructure of SPI gel. Results showed that SPI gel was a typical viscoelastic material with strong frequency dependence. With the increasing of XG concentration (1%~5%), the elastic modulus and viscous modulus of the mixed gel increased, and the loss tangent decreased, and the gel strength increased. The addition of XG affected the self-healing ability of SPI gel, although the healing time was prolonged, the recovery rate increased. The SPI single gel which was heated (25~100 ℃) occurred a transition of gel-sol state and it structure was extremely damaged. Comparatively, the thermal stability of XG-SPI mix gel increased significantly. The microstructural changes of SPI single gel and XG-SPI mixed gel were not observed under electron microscope. The results of this study could provide important information for developing new soy protein isolate gels with improved quality.

By examining field outcrops, drilling cores and seismic data, it is concluded that the Middle and Late Permian “Emeishan basalts” in Western Sichuan Basin were developed in two large eruption cycles, and the two sets of igneous rocks are in unconformable contact. The lower cycle is dominated by overflow volcanic rocks; while the upper cycle made up of pyroclastic flow volcanic breccia and pyroclastic lava is typical explosive facies accumulation. With high-quality micro-dissolution pores and ultra-fine dissolution pores, the upper cycle is a set of high-quality porous reservoir. Based on strong heterogeneity and great differences of pyroclastic flow subfacies from surrounding rocks in lithology and physical properties, the volcanic facies and volcanic edifices in Western Sichuan were effectively predicted and characterized by using seismic attribute analysis method and instantaneous amplitude and instantaneous frequency coherence analysis. The pyroclastic flow volcanic rocks are widely distributed in the Jianyang area. Centering around wells YT1, TF2 and TF8, the volcanic rocks in Jianyang area had 3 edifice groups and an area of about 500 km2 which is the most favorable area for oil and gas exploration in volcanic rocks.

Objective To assess three severe acute respiratorysyndrome coronavirus 2 (SARS-CoV-2) enzyme linked immunosorbent assays (ELISA) and one pseudotype lentivirus-based neutralization test (ppNAT) in detecting the convalescent plasma antibody levles from COVID-19. Methods 30 COVID-19 convalescent plasma samples were screened for antibodies against SARS-CoV-2 using three kinds of SARS-CoV-2 ELISA reagents and one ppNAT test in Shenzhen. The controls consisted of plasma samples from 32 healthy blood donors in February 2019. The diagnostic efficacy analysis of various SARS-CoV-2 ELISA reagents was performed using real-time fluorescent Polymerase Chain Reaction (RT-PCR). We also analyzed correlation between different immunological reagents and the age, gender, hospitalization, and severity of illness. Results The positive yielding rate of ppNAT and three kinds of IgG ELISA was higher than that of IgM ELISA. The positive yielding rates of three kinds of IgG ELISA were 100%(30/30), 93.33%(28/30), and 96.67%(29/30) respectively, while the yielding rates in control group were all 0. The positive yielding rate of three IgM ELISAs were 93.33%(28/30), 70%(21/30)and 46.67% (14/30). All the cases from negative control group were negative for IgG and IgM. Pearson correlation coefficient was calculated; there was a strong correlation between ELISA reagent 2 IgG and ELISA reagent 3 IgG (r=0.765, P0.05). Conclusion In the convalescent plasma with nucleic acid confirmed covid-19, the yielding rates of different IgM antibodies varied greatly. Antibody levels were influenced by age to some extent.

Objective To evaluate the performance and clinical application of a high-throughput nucleic acid blood screening detection system for SARS-CoV-2, so as to provide basis and technical means for its application in detection of plasma from recovered COVID-19 patients. Methods The SARS-CoV-2 nucleic acid was detected by real-time fluorescence quantitative PCR, and the sensitivity, precision, anti-interference and other parameters were evaluated. Blood donor samples collected during COVID-19 epidemic period were screened using the detection system to evaluate its applicability. Results The detection limits of gene N and ORF 1ab were 3.98 copies/mL and 9.38 copies/mL, respectively. The CV of high and low concentration samples were both less than 5%. Hemoglobin at 500 mg/dL and triglyceride at 3g/dL had little effect on the results. The detection system can effectively prevent carryover, thus avoiding false positive results. The SARS-CoV-2 nucleic acid blood screening was carried out in a total of 39 306 blood samples, and all samples were negative. Conclusion The established method can meet the needs of SARS-CoV-2 nucleic acid screening therefore ensure the safe application of plasma from recovered COVID-19 patients.

This study aimed to accurately identify the “golden flower” fungus, and characterize its effects on the chemical composition of Fu tea. A “golden flower” fungus strain was isolated from Hunan Fu tea and named as ACF-1. The ACF-1 was identified as Aspergillus cristatus using culture characteristics, optical microscopic structure, electron microscopic structure, ITS region sequence alignment analysis, and multigene phylogeny analysis based on β-tubulin, calmodulin, and RNA polymerase Ⅱ gene sequences. ACF-1 fungus was inoculated into sterilized primary dark tea for a loose tea “flowering” experiment, to analyze changes in the main chemical components. Polyphenol, free amino acid, soluble sugar, and flavonoid contents changed substantially in the “flowering” samples compared to non-“flowering”, decreasing by 11.3%, 17.3%, 11.9%, and 33.8%, respectively. The variation of chemical components made a foundation for the quality improvement during the process. These findings provide a more comprehensive understanding of the formation of the sensory characteristics in Fu tea and provide a guidance for the development and utilization of “golden flower” fungus.

Considering the energy and environmental issues faced by human society, hydrogen has become increasingly important, and electrocatalytic water splitting is considered to be an ideal way to solve these energy issues. However, although most electrocatalysts will undergo a structural evolution when in service conditions, our understanding of the service behavior of catalysts is limited. To design highly active catalysts, operando characterization techniques must be used to study their dynamic structural evolution. Today, the development of synchrotron radiation devices has reached an important stage. Synchrotron-radiation-based X-ray characterization, which has high energy, large flux, and excellent collimation compared with the ordinary laboratory X-ray source, can capture the precise structure of catalytic materials. In this review, we present the development status of synchrotron radiation devices and the basic principles of operando X-ray absorption spectroscopy, X-ray diffraction spectroscopy, and X-ray photoelectron spectroscopy based on synchrotron radiation. In addition, we highlight studies related to the dynamic service behavior of water-splitting catalysts under real conditions and list a variety of operando studies of typical water-splitting catalysts, including NiFe hydroxide/(oxy)hydroxides, perovskite oxides, spinel oxides, and noble-metal-based catalysts. The use of operando X-ray techniques deepens our understanding of the catalyst reaction mechanism and provides a basis for identifying the dynamic structure–performance correlation of catalysts. We summarize the problems and challenges of operando X-ray-based techniques in complex electrochemical environments and propose the prospect of an advanced synchrotron radiation facility for operando X-ray characterization. With the development of the next-generation synchrotron radiation facility, adequately using this advanced X-ray light source to study the dynamic structure–activity correlation of catalytic materials throughout their life cycle to achieve the precise design and synthesis of complex pre-catalysts will advance the development of this field by enabling greater refinement and control.

Based on outcrop, drilling, logging, geochemical analysis and seismic data, the karst landform and distribution of Permian volcanic rocks at the end of the sedimentary period of the Maokou Formation in the western Sichuan Basin are examined, and their petroleum geological significance is discussed. Affected by normal faults formed in the early magmatic activities and extension tectonic background in the late sedimentary period of the Maokou Formation, a local karst shallow depression under the background of karst slope came up in the Jianyang area of the western Sichuan Basin, where the residual thickness of the Maokou Formation was thinner. Basic volcanic rocks like pyroclastic rock of eruptive facies, basalt of overflow facies, diabase porphyrite of intrusive facies and sedimentary tuff of volcanic sedimentary facies were formed after karstification. However, under the effects of faulting and karst paleogeomorphology, the volcanic rocks in different areas had different accumulation features. In the Jianyang area, with long eruption time, the volcanic rocks were thick and complex in lithology, and accumulated in the karst depressions. In the Zhongjiang-Santai area located in the karst slope, there’s no fault developed, only thin layers of basalt and sedimentary tuff turned up. The karst landform controls the build-up of thick explosive facies volcanic rocks and also the development of karst reservoirs in the Maokou Formation, and the western Sichuan area has oil and gas exploration potential in volcanic rocks and the Maokou Formation.

ObjectiveTo investigate the application value of narrative medical model in communication with cancer patients with low education level.MethodsRetrospective analysis was performed on 80 cancer patients with low education level who were hospitalized in West China Hospital of Sichuan University from March 2019 to April 2020. They were randomly divided into control group （n=40） and observation group （n=40）. The control group received conventional nursing intervention， while the observation group received narrative medical nursing mode intervention. Both groups received intervention for 6 months. The Self-rating Anxiety Scale （SAS）， Self-rating Depression Scale （SDS）， Morisky Medication Adherence Scale （MMAS-8）， Social Support Rating Scale （SSRS） and self-designed knowledge of the disease and cognition questionnaire were used for evaluation before and after intervention.ResultsAfter intervention， the SAS and SDS scores of observation group were lower than those of control group （t=7.493， 8.314， P

Objective To understand the prevalence, drug resistance, and virulence gene distribution of foodborne Staphylococcus aureus in northwestern Hubei Province. Methods A total of 303 food samples were collected from Xiangyang City, Shiyan City, and Suizhou City of Hubei Province for Staphylococcus aureus screening. Toxic genes and methicillin-resistant Staphylococcus aureus (MRSA) were detected with PCR method. The drug resistance of Staphylococcus aureus was determined with K-B paper diffusion method. Results Staphylococcus aureus strain was determined from 41 samples with the positive rate of 13.53%. Among them, the highest detection rate was from raw meat products (23.91%, 22/92). Among the enterotoxigenic strains, the strain carrying sea was the most common, accounting for 87.80% (36/41). The strains carrying eta and tst accounted for 97.56% (40/41) and 7.32% (3/41), respectively. Strains carrying three or more enterotoxin genes accounted for 17.07% (7/41). 2.44% (1/41) of strains carry eta and tst simultaneously. The drug susceptibility result showed that the penicillin, tetracycline, erythromycin, and doxycycline accounted for 78.05% (32/41), 43.90% (18/41), 31.71% (13/41) and 21.95% (9/41) respectively. The mecA gene test showed that 19.51% (8/41) of the strains were MRSA strains. Conclusion Foodborne Staphylococcus aureus in northwestern Hubei Province had a higher detection rate, toxic gene carring rate, and multiple drug resistance. Related departments need to strengthen food safety monitoring to control the spread of the strain.

Cerebral amyloid angiopathy associated with inflammation (CAA⁃I) is a rare subtype of cerebral amyloid angiopathy (CAA), and is increasingly recognized by neurologists. CAA⁃Iischaracterized by the pathological hallmarks of both CAA and vasculitis. The main clinical manifestations contain cognitive decline, seizures, headaches and so on, which present great clinical heterogeneity. Cranial MRI plays an important role in the diagnosis and evaluation of CAA⁃I, by which white matter hyperintensity (WMH) and multiple cerebral microbleeds (CMBs) could be usually found. CAA⁃I responds well to immunosuppressive therapy. All the characteristics above are different from CAA somehow. This paper aims to review the advances in pathophysiology, pathology, clinical manifestations, laboratory examination and treatment of CAA⁃I. DOI：10.3969/j.issn.1672⁃6731.2020.09.002

The Ni-Cr-P metal powder was prepared by vacuum melting and inert gas atomization method, and then an organic binder was added and stirred at high speed to prepare a Ni14Cr10P paste-like filler metal. The prepared pasty filler metal was used to braze the C/C composites in a vacuum furnace. Then the shear strength of the brazed joint was tested. The interface structure of the brazed joint was analyzed by OM, SEM, EDS and XRD. The results show that the shear strength of the joint obtained at the brazing temperature of 1000℃ and the holding time of 0.5 h reaches 28.6 MPa, and then the strength of the brazed joint is decreased with the increase of the brazing temperature or the holding time. It is found that the contact area between the brazing layer and the surface of the C/C composites is increased by the pasty brazing filler metal, which is favorable for blocking the pores on the surface of the C/C composites. A staggered distribution of Cr carbide phase buffer layer is formed at the interface, so that the interface exhibits a structure with an increased thermal expansion coefficient gradient, which helps to alleviate the thermal mismatch and improve the strength of the brazed joint.

Traditional distributed network architecture constraints the innovation of routing algorithm.Software-defined network (SDN) provides a new solution for the optimization of routing algorithm.Previous researches show that the quality of service (QoS) routing issues are based on heuristic algorithm mostly,but these methods cannot be applied in large networks due to their high computing complexity.However,other algorithms have a lot of problems,which are high complexity or poor QoS performance,such as shortest path algorithm.This paper proposes A new QoS routing algorithm:LC-LD routing algorithm was proposed.LC-LD was based on SDN west-east interface and binds both delay constraint and cost constraint.keeping a good balance between computational complexity and algorithm performance.Finally,the simulation results show that LC-LD can possess both low computational complexity and high QoS routing performance.

To address the high rock strength and low drilling rate issues in deep oil/gas and geothermal exploitation, we performed mechanical property tests on three kinds of rock samples (granite, shale and sandstone) subjected to liquid nitrogen (LN2) cooling and conducted rock-breaking experiments using LN2 jet. Rock-breaking characteristics and mechanisms of LN2 jet, heat transfer features between LN2 and rock and thermal stress evolution in rock were analyzed. A novel high-pressure LN2 jet assisted drilling method was proposed accordingly. The study results show that LN2 thermal shock can significantly reduce uniaxial compression strength and elastic modulus of rock. Rock damage and corresponding mechanical deterioration become more pronounced with increasing rock temperature. The LN2 jet has merits of high rock-breaking efficiency and low threshold rock-breaking pressure. Rock failure under LN2 jet impact is characterized by large volume breakage and the rock-breaking performance becomes more significant with increase of rock temperature. Under the impact of LN2 jet, the damage of granite is the most remarkable among the three rock samples. Thus, this method works better for high temperature granite formations. It has a good application prospect in speeding up drilling rate in deep hot dry rock geothermal reservoirs. Key words: liquid nitrogen jet, cold shock, jet impact, rock-breaking experiment, assisted drilling, drilling rate improvement

A 3D coupled mathematical model of 1 000 MW ultra-supercritical tower boiler was established. Based on the design parameters of the boiler, the model calculated the flow and heat transfer process of the boiler in operation. The model was used to simulate different load conditions of the supercritical boiler. The boiler maximum continuous rating (BMCR) condition, 75% BMCR condition and 50% BMCR condition were selected to explore the heat transfer during peak shaving operation. By analyzing the temperature and heat flux distribution in the furnace and the temperature distribution in the tube and on the water cooled wall, it is found that the temperature and heat flux change of the BMCR, 75% BMCR are in the similar trend while the temperature change of the 50% BMCR is irregular, and the heat transfer deterioration is prone to occur in the spiral water cooled wall region.

Objective To investigate the association of the interaction between brain-derived neurotrophic factor (BDNF) gene and neurotrophic tyrosine kinase receptor type 2 (NTRK2) gene with suicide attempt in adolescents. Methods One single nucleotide polymorphism (SNP) of BDNF gene (rs1491851) and 3 SNPs of NTRK2 (rs1147198, rs11140800, rs1565445) were tested by IMLDRtm multiplex SNP typing in 70 suicide attempters (15~24 years old) and 111 healthy young individuals (control). Analysis on gene-gene interaction was conducted by generalized multifactor dimensionality reduction (GMDR). Results No significant differences were found in genotype frequencies and allele frequencies between the attempters and controls at 4 SNPs (BDNF rs1491851, and NTRK2 rs1147198, rs1140800, rs1565445)(P>0.05). GMDR analysis indicated the interactions between BDNF gene and NTRK2 gene included a two-locus combination (rs1491851, rs1147198), a three-locus combination (rs1491851, rs1147198, rs11140800) and four-locus combination (rs1491851, rs1147198, rs11140800, rs1565445). The four-locus combination was identified as the best model (Bal. Acc. CV test: 0.632 5, CVC consistency: 10/10, permutation test: P=0.032~0.033). Conclusion There are interactions between BDNF gene and NTRK2 gene, which may be associated with suicide attempt in adolescents.

A network selection algorithm based on synergetic for heterogeneous wireless network was proposed. The algorithm considered the network selection as a systematic problem. The optional network was a composite system and each kind of service running on the terminal was a subsystem. The entropy of the composite system was obtained according to the concept of entropy and it can reflected the degree of coordination among the subsystems, which in turn reflected the overall performance of the network. The simulation results show that the QoS satisfaction rate of services based on the proposed algorithm is 95%, while the QoS satisfaction rate of services based on the TOPSIS algorithm is only 25%, and the performance of the former is improved by nearly four times, indicating that the problem of multi-service terminal network selection can be solved by the proposed algorithm.

Objective To investigate the expressions and clinical significance of serum anti - acetylcholine receptor antibodies (AChR-Ab), anti-Titin antibodies (Titin-Ab), and anti-Ryanodine receptor antibodies (RyR-Ab) in patients with myasthenia gravis (MG). Methods Serum AChR-Ab, Titin-Ab and RyR-Ab were detected with enzyme-linked immunosorbent assay (ELISA) in 182 MG patients, 105 patients of other neurological diseases (OND) and 62 normal controls. Results Serum AChR-Ab, Titin-Ab and RyR-Ab were detected positive respectively in 68.13% (124/182), 64.29% (117/182) and 67.03% (122/182) of patients in MG group. The positive rates of those antibodies in MG group were significantly higher than those in OND group (P = 0.000, for all) and control group (P = 0.000, for all). When 3 antibodies coexisted, the sensitivity in the diagnosis of MG was 41.21%, with 99.40% of specificity. There was no significant difference in the positive rate of serum AChR-Ab, Titin-Ab and RyR-Ab between early-onset MG subgroup and late-onset MG subgroup (P > 0.05, for all). The positive rates of AChR-Ab were significantly higher in MG patients with thymoma than in those without thymoma (P = 0.004). There was no significant difference in the positive rate of Titin-Ab and RyR-Ab between MG with thymoma subgroup and MG without thymoma subgroup (P > 0.05, for all). The prevalence of AChR-Ab in generalized MG (GMG) atients (Ⅱa and Ⅱb) was higher than those in ocular MG (OMG) patients (typeⅠ; P = 0.005, 0.012). There was no significant difference in the positive rate of Titin-Ab and RyR-Ab between GMG subgroup and OMG subgroup (P > 0.05, for all). Conclusions Serum AChR-Ab, Titin-Ab and RyR-Ab can be used as ndicators of the diagnosis of MG. Patients with 3 coexisted positive antibodies are highly suspected as MG. Higher AChR-Ab level in serum of OMG patients indicates the possibility of progressing to GMG. DOI: 10.3969/j.issn.1672-6731.2016.10.007

Inflammatory reaction plays a crucial role in the occurence and development of atherosclerosis. Both basic and clinical trials have provided evidence that the expression of inflammatory biomarkers are closely related with the degree of atherosclerosis. Treatment towards inflammatory factors would bring benefit to atherosclerotic patients. This review highlighted the mechanistic rationale and specific therapies targeting traditional and novel inflammatory biomarkers, including C-reactive protein (CRP), interleukin-17 (IL-17), secretory phospholipase A2 (sPLA2), lipoprotein-associated phospholipase A2 (Lp-PLA2), endoglin, chemokine receptor and 5-lipoxygenase (5-LO), so as to review its mechanism of action and treatment prospect. DOI: 10.3969/j.issn.1672-6731.2016.09.004

To overcome the deficiencies of the material balance method, according to strong heterogeneity of tight oil reservoirs, the flow region of fractured horizontal well is divided into high permeable zone and low permeable zone, which are equivalent to radial composite percolation model. Based on parallel plane theory, multiple media of each zone are equivalent as a continuous medium, and with the integral method, the multi-region material balance to calculate the dynamic reserves for the fractured horizontal well of tight oil reservoirs is proposed base on the nonlinear seepage mechanism of tight oil reservoirs, and the corresponding pressure distribution equation and material balance equation for the two zones have been established. In view of the actual production performance, this method considers the pressure mutation and fluid exchange at the interface of two zones. The computational results of an example show that this method can work out the dynamic reserves within a single well control, the dynamic reserves of high permeable zone and low permeable zone, and the recharge rate from the low permeable zone to the high permeable zone in different production time accurately, which provides a basis for selection of well production and appropriate working system, and deployment and adjustment of development well pattern. Key words: tight oil, fractured horizontal well, pressure distribution, dynamic reserves, multi-region, material balance method

In order to study the novel green organic mercury-substituting inhibitors, the ionic liquid surfactant 1-methyl-3-dodecyl imidazole bromide ( Br) is synthesized with N-methyl imidazole and 1-bromodecane as raw materials. The corrosion inhibition of Br for zinc in zinc-manganese batteries is investigated using electrochemical methods and weight loss methods. The results show that corrosion inhibition efficiency increases with the increase of the concentration of Br, and when the concentration is higher than 8×10-3 mol/L, the inhibition efficiency tends to be stable. The polarization curve shows that Br belongs to mixed-type corrosion inhibitor. The thermodynamic parameters show that Br is spontaneously adsorbed on the zinc surface, forming a monomolecular adsorption layer, which fits with Langmuir adsorption isotherm with physical and chemical adsorption mechanism.

Objective To explore the relationship between mean arterial pressure (MAP) on admission and prognosis of patients with acute ischemic stroke. Methods A total of 342 patients on acute stage (< 24 h) of ischemic stroke were divided into 4 groups according to their MAP levels on admission: < 97 mm Hg, 97- mm Hg, 107- mm Hg and ≥ 117 mm Hg. Ninety days after onset, these patients were divided into 2 groups according to modified Rankin Scale (mRS): favorable prognosis (mRS < 3) and unfavorable prognosis (mRS 3-6). Logistic regression analysis was used to evaluate the effect of MAP at admission on the prognosis 90 d after onset. Results Patients with unfavorable prognosis in 4 MAP groups (< 97 mm Hg, 97- mm Hg, 107- mm Hg and ≥ 117 mm Hg) accounted for 34.78% (16/46), 14.29% (14/98), 15.32% (17/111) and 41.38% (36/87), respectively. Patients in 97- mm Hg and 107- mm Hg groups presented better prognosis (P < 0.01, for all). In Logistic regression analysis, by optimal modification of systolic blood pressure (SBP) and diastolic blood pressure (DBP), MAP from 97 mm Hg to 107 mm Hg and from 107 mm Hg to 117 mm Hg were independent protective factors for the prognosis 90 d after onset (P = 0.003, 0.011). Conclusions Too high or too low MAP in acute stage of ischemic stroke indicates an unfavorable progonsis of patients. Therefore, MAP can be used as predictive indicator of prognosis of patients with acute ischemic stroke. DOI: 10.3969/j.issn.1672-6731.2015.05.013

Based on comprehensive analysis of deposition and structure, the horizontal and vertical characteristics of the Weiyuan-Anyue erosion-extension groove have been studied by outcrop, drilling, well logging and seismic data. According to the strata, lithology, structure and regional evolution, Sinian system before the erosion has been reconstructed to show the development and transformation of the erosion-extension groove in the Tongwan movement. The Weiyuan-Anyue tensional corrosion trough is distributed along the Penglai-Anyue-Rongchang in NW direction, with the west area steeper than the east in groove. According to the study of the Weiyuan-Longnüsi seismic profile across the middle area of erosion-extension groove, the formation of the Weiyuan-Anyue tensional corrosion trough is the synthetic result of different geological processes from differential uplift, differential erosion and dissolution in the Dengying Formation caused by the multiple-phase episode grabon or horst movement of the basement fault in Sinian. The formation of the Weiyuan-Anyue erosion-extension groove can be divided into three stages, and the erosion and dissolution can be calculated quantitatively by denudation recovery profile, its maximum can reach 600 m. Key words: Sichuan Basin, Sinian, Weiyuan-Anyue, tensional corrosion trough, formation and evolution

Autoantibodies play an important role in autoimmune diseases of the central nervous system. This article summarizes the autoantibodies involved in multiple sclerosis (MS), neuromyelitis optica (NMO) and autoimmune encephalitis, and their clinical significance. doi: 10.3969/j.issn.1672-6731.2014.09.005

Traditional block compressed sensing (BCS) of images uses the same measurement rate to measure each block,but some blocking artifacts appear in the reconstructed image on accounting of varying spatial characteristics in an image.This problem can be effectively solved by adaptively setting different measurement rate for every block.However,these existing methods require original digital image at the collector,which cannot be realized by using practical compressive imaging (CI) devices.In order to overcome this shortage,an adaptive measurement rate setting method is proposed and it can be easily achieved though hardware equipments.This method uses the CS measurements acquired at the collector to estimate the sample variance of each block directly,and then adaptively sets measurement rate of each block in terms of their sample variances and realize rate control.Experimental results show that proposed method can obtain a better qual-ity of reconstructed image than non-adaptive scheme,but there is a gap between proposed method and the adaptive scheme using the true block sample variance since the sample variance estimated in the measurement domain has some deviations.

Based on the fundamentals and advantages of fuel cell backup powers, the innovation and enhance of the fuel cell backup power over general backup power is technically and economically discussed and analyzed. An outdoor telecommunication cabinet solution with fuel cell backup power is illustrated and further research direction on energy saving and emission reduction from fuel cell power in telecom application is suggested.