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Copyright of Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao is the property of Chinese Journal of Preventive Veterinary Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objective] This study aimed to prepare rabbit polyclonal antibody against Toll-like receptor 5 (TLR5) of Bactrian camel and identify its activity. [Method] The TLR5 gene sequence of Bactrian camel was targeted, the coding region was determined, and the amino acid site 1 -499 was selected as the main resistance site by software analysis. The corresponding nucleotide sequence was selected for gene synthesis and connected with pET-28a (+) vector to construct the recombinant plasmid pET28a-TLR5. The recombinant plasmid pET28a-TLR5 was identified by Bam H I and Xho I double digestion. PET28a-TI.R5 was transfected into E. coli prokaryotic expression system, and the expression was induced by IPTG. The concentration of IPTG (0.1, 0.2, 0.4, 0.6, 0.8, 1.0 mol/L) and induction time (2, 3, 4, 5, 6, 7, 8, 9 h) were then optimized. Expressed protein was purified by affinity chromatography and immunized with New Zealand white rabbits to prepare polyclonal antibodies. The titer of polyclonal antibodies was detected by ELISA, and the specificity of polyclonal antibodies was verified and evaluated by Western blot detection and immunohistochemistry. [Result] Recombinant plasmid pET28a-TLR5 was successfully constructed. The recombinant protein (about 57 ku) was obtained after induction by 1PTG and expressed in the form of inclusion bodies. The best induction concentration of 1PTG and induction time were 1 mol/L and 6 h, respectively. The titer of TLR5 polyclonal antibody was 1 : 128 000. The Western blot showed that TLR5 polyclonal antibody could specifically recognize the target protein, and immunohistochemical results showed that TLR5 polyclonal antibody could well recognize TLR5 positive cells in mesenteric lymph of adult Bactrian camel. [Conclusion] The rabbit anti-Bactrian camel TLR5 polyclonal antibody was successfully prepared, which can be used for the detection of TLR5. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao is the property of Chinese Journal of Preventive Veterinary Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao is the property of Chinese Journal of Preventive Veterinary Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To investigate the efficacy and safety of disintegrin DTY29 on inhibiting the proliferation of rabbit lens epithelial cells (LECs) in vitro and in vivo. Methods (l) In vitro: 36-well plates were divided into control group (a) and experimental groups (b, c and d), nine holes each group. The human LECs were cultured and scratched, and then culture media, 0.08 µmol/L, 0.4 µmol/L, and 2 µmol/L DTY29 were added into 4 groups, respectively. Scratch widths were photographed and measured at 0 h, 6h, 12h and 24 h. (2) In vivo: 36 male New Zealand white rabbits were randomly divided into control group (A), experimental groups (B, C and D), nine rabbits each group. Amounts of corneal endothelial cells were calculated before operative. Phacoemulsification was performed in all right eyes of the rabbits, and then 0.2 mL of Ringer's, 10 µ mol/L, 20 µmol/L and 40 µmol/L DTY29 solution was injected into the anterior chamber of groups A, B, C and D, respectively. Cornea, anterior chamber, pupil, posterior capsular opacification (PCO), and fundus were observed postoperatively. Results At6h, 12 h and 24 h of cells scratch test in vitro, the scratch widths of group a reduced significantly (P < 0.05) compared with groups b, c and d. There were no significant differences in corneal endothelial cells loss among groups A, B and C (P > 0.05), while a significant reduction was found in group D compared with groups A,B and C (< 0.05). PCO was observed in both groups A and B after 1-3 months postoperatively, but there was no significant difference in incidence (> 0.05). PCO hadn't occurred in groups C and D. Retinal detachment,choroidal detachment and other serious complications were not observed. Conclusion Disintegrin DTY29 could inhibit lens epithelial cells proliferation in vitro or in vivo,and there was a positive correlation between inhibiting effect and concentration. In vivo, corneal endothelial cells can be destroyed by DTY29 with a high concentration, and no side effects on intraocular tissues have been observed, which demonstrated the safety at a low concentration. The experiment results suggested that the optimum concentration ranged from 10 µmol/L to 20 µmol/L,and further investigation should be done to prove its safety and optimum concentration. [ABSTRACT FROM AUTHOR]

Objective To observe the expression of connexins of atrial myocardium that increases with age and investigate the therapeutic effects of captopril on age-related changes. Methods Healthy rabbits were divided into three groups with 8 in each group: adult (4-6 months), old (30-34 months) and old+captopril. After 8 weeks of intervention, eletrophysiological markers P wave average duration (Pa) and P wave dispersion (Pd) were recorded in each group. Cardiac angiotensin (Ang)Ⅱ levels were assessed by a commercial ELISA kit. The expressions ofCx43 and Cx40, the two atrial connexins, were analyzed at the mRNA and protein levels by real-time PCR and Western blot in the left atrial appendages from rabbits of both groups. Results ① The ECG result of the old rabbits manifested longer Pa and Pd than those of the adult ones (both P<0.01). ② AngⅡ accumulation was higher in the old group than in the young group (P<0.01). The gene and protein levels of Cx40 and Cx43 expressions decreased in the atrium of old rabbits compared with the adults (P<0.01). ③ Compared with those in the old group, the Pd and Pa in the captopril group were all significantly shorter (P<0.05). Captopril increased the mRNA and protein expressions of Cx40 and Cx43 in the old rabbits (<0.05). Conclusion Aging markedly prolongates Pa and Pd of atria, which predicts the development of AF. The age-related decrease of gene and protein expressions of Cx40 and Cx43 may be the molecular mechanism of AF due to aging. Captopril's reversing the age-associated changes may contribute to preventing AF. [ABSTRACT FROM AUTHOR]

Objective To investigate the effect of piogiltazone on the expression of adiponectin in aortic atherosclerosis (AS) plaque area in rabbits and explore the anti-AS mechanism of piogiltazone. Methods Totally 36 New Zealand rabbits were randomly divided into 3 groups: control group, high-cholesterol diet group, and high-cholesterol diet + pioglitazone group. All animals were fed for 8 weeks and then sacrificed. The aortic atherosclerotic lesion area, intimal thickness and intimal/media thickness ratio were measured by light microscopy. By Masson staining, rabbit aortic AS pathological features were observed; the expression of adiponectin of aortic AS plaque area in rabbits was detected by immunohistochemistry. Results In the high-cholesterol diet + pioglitazone group, aortic AS plaque area ratio, intimal thickness, and intima/media thickness ratio were all significantly higher than those in the control group but smaller than those in the high-cholesterol diet group (all P<0.05). The protein expression of adiponectin was higher than that in the high-cholesterol diet group but lower than that in the control group (P<0.05). Conclusion Pioglitazone has an anti-AS effect and can up-regulate the protein expression of adiponectin in AS plague. [ABSTRACT FROM AUTHOR]

PURPOSE: To identify the vascular endothelial growth factor receptors (VEGFRs) expression in the rabbit synoviums with temporomandibular joint (TMJ) internal derangement (ID). METHODS: Real-time PCR was used to detect VEGF receptor expression in synovium, after establishing the rabbit TMJ ID model for 1, 2, 3, or 4 weeks. VEGFRs mRNA expression was normalized by comparing VEGFRs mRNA with internal control GAPDH. SPSS13.0 software package was used for comparison of the differences between the two groups. RESULTS: In the first and fourth week, significant difference was observed in VEGFR-2 mRNA expression between the experimental group and control group; however, no statistical difference was found in VEGFR-1 and VEGFR-3 mRNA expression between the experimental group and control group. In the second and third week, there were no statistical differences in VEGFR-1, VEGFR-2, and VEGFR-3 mRNA expression between the experimental group and control group. CONCLUSION: VEGFR-2 may play an important role in TMJ ID and adhesion formation. Specific VEGFR-2 inhibitors may be used to prevent adhesion formation in TMJ ID. Supported by Research Fund of Science and Technology Commission of Shanghai Municipality (Grant No. 08DZ2271100), Grant of Shanghai Leading Academic Discipline Project (Grant No.S30206), Grant of Shanghai Municipal Bureau of Health (Grant No.2008160), Grant from Shanghai "Phosphor" Science Foundation (Grant No.04QMH1415), Grant from Ph.D. Creative Foundation of Shanghai Jiao Tong University School of Medicine (Grant No.BXJ0926), and Research Fund of Medicine and Engineering of Shanghai Jiao Tong University (Grant No.YG2009MS42). [ABSTRACT FROM AUTHOR]