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Objective To investigate the significance of transcription factor LIM domain only 3 (LMO3) expression level in the prognosis of patients with endometrial carcinoma (EC). Methods The Cancer Genome Atlas (TCGA), GeneMANIA and STRING databases were used to analyze the relationship between LMO3 expression in EC tissues and the prognosis of patients, as well as the genes interacting with LMO3, and the correlation of LMO3 with TP53 mutation. The paraffin sections and clinical information of 51 patients with EC who underwent surgery in our hospital from March 2011 to December 2014 were collected. Immunohistochemical staining was subsequently performed to determine the expression of LMO3 in EC tissues and its association with the clinicopathological features of patients. SPSS was used to plot the survival curve, and COX multivariate regression analysis was also adopted. In addition, LMO3 overexpression was conducted in EC cells to verify its effect on tumor cell migration. Results According to the analysis of TCGA database, the overall survival and disease-free survival of patients with high LMO3 expression were significantly shorter than those of low LMO3 expression(P < 0.05), and the expression level of LMO3 in patients with TP53 mutation was remarkably higher than that in TP53 wild-type patients. GeneMANIA and STRING analyses showed that the main genes interacting with LMO3 included NHLH2 and LDB2. Moreover, immunohistochemical staining also indicated that patients with higher LMO3 expression level had shorter disease-free survival (P=0.005), and multivariate COX analysis suggested LMO3 as a prognostic factor for EC. Finally, overexpression of LMO3 enhanced the migration of EC cells. Conclusion EC patients with high LMO3 level have a poor prognosis, which may be related to the migration of EC cells promoted by LMO3 overexpression.

Objective: This study aimed to investigate the protective effect and mechanism of solid beverage with vine tea combined with and cassia on alcoholic liver injury in rats. Methods: SD rats were given 43%vol aging by gavage to establish model of alcoholic liver injury. The model was fed with low, medium and high doses of solid beverage. The appropriate ratio of solid beverages was detected by cell test, the physiological and biochemical indicators of rats were detected by using kits, and the expression of related differential genes was studied by transcriptomic analysis to assessing the effect of vine tea extract combined with cassia extract solid beverage on alcoholic liver injury in rats and revealing the mechanism of action. Results: Compared with the model group, the activities of ALT, AST and LDH in the solid beverage group were significantly decreased; the levels of IL-6 and TNF-α was significantly reduced; the activities of GSH and SOD in the liver were significantly increased, while the contents of MDA and TG were significantly decreased; finally the oxidative stress and inflammatory response in rat liver were significantly improved. Compared to the model group, the inflammatory cell infiltration and vacuolar degeneration of liver cells in the liver pathological sections of the solid beverage group were significantly reduced; transcriptomic analysis speculated that the regulation of genes such as spermidine synthase, Sirt7, RT1-A2, RT1-CE1 and other pathways and amino acid metabolism are related to vine tea extract combined with cassia extract solid beverage to alleviate alcoholic liver injury. Conclusion: Vine tea extract combined with cassia extract solid beverage can alleviate alcohol-induced liver injury by improving liver function, reducing liver inflammation and oxidative stress.

DOI: 10.3969/j.issn.1672-6731.2019.03.013

Tensile test was conducted on 2.25Cr-1Mo steel specimens that were electrochemically hydrogen charged and acoustic emission signals were collected in the real-time stretching process. The results show that tensile strength of 2.25Cr-1Mo steel with hydrogen charging is 536.30MPa, decreased by approximately 57MPa and reduction of area is 43.62%, decreased by 7%, compared with the specimens without hydrogen charging. Hydrogen embrittlement-induced regions known as "fisheyes" and quasi-cleavage morphology are observed on the tensile fracture surface. The AE signals activity in the elastic stage of hydrogen charged 2.25Cr-1Mo steel is enhanced, but the acoustic emission signals activity in the yield stage is decreased. The cumulative absolute energy of AE signals of hydrogen charged specimens during deformation is almost one order magnitude lower than that without hydrogen charged specimens. The AE signals amplitude generated by the hydrogen charged specimens is about 0.33mV lower than that of the specimens without hydrogen charging during tensile deformation, and that the bandwidth of signal is reduced by 0.06MHz. Though the analysis of AE signals, it is found that tensile deformation microscopic mechanism of hydrogen charged specimens is that dislocation emission and motion is enhanced but dislocation cross-slip is inhibited by hydrogen.

Energy efficiency analyses of automatic repeat request （ARQ） and forward error correction （FEC） techniques were presented. Chase combining hybrid ARQ （HARQ） was proposed to achieve adaptive error control in wireless sensor networks. Moreover, Chase combining HARQ was compared with ARQ and FEC in terms of energy efficiency based on different communication distances and packet lengths. The simulation result shows that Chase combining HARQ is the optimal scheme on the whole.

Aiming at the problems of unstable strain sensing performance, small tensile range and low sensitivity of the existing flexible strain sensor, the conductive silver nanowires (AgNWs) are synthesized and combined with the elastic fabric to prepare the flexible strain sensor. The conductive network is formed under low filling amount to improve the conductivity of elastic fabric at the initial strain. At the same time, the interface layer formed by water-based resin has the effect of bonding agent in the strain process of elastic fabric, effectively improves the sensitivity and stability of the strain sensor. The micro morphology of long silver nanowires and fiexible strain sensor are observed, and the tensile sensing performance of flexible strain sensor is investigated. The results show that the prepared flexible strain sensor has a strain range of 0-60% and a maximum sensitivity of 107, which can be used for the detection of human tiny motion. It still has stable strain sensing performance in static stretching and hundreds of dynamic repeated stretching tests. [ABSTRACT FROM AUTHOR]

To understand the short-term response of soil CH4 flux to different precipitation events in a winter wheat field on the Loess Plateau, we conducted precipitation simulation experiments during winter wheat jointing stage and summer fallow period to measure the soil CH4 flux in 0 to 72 h after precipitation simulation of 1 to 32 mm. Results showed that CH4 flux during 0 to 72 h fluctuated at 1 to 8 mm of precipitation application and peaked at 16 and 32 mm. Cumulative CH4 flux after 72 h of precipitation (CH4-C) increased linearly with the precipitation amount (P) (wheat jointing stage: CH4-C=2.45P-6.09, R2=0.92, P<0.01; summer fallow: CH4-C=2.43P-4.73, R2=0.91, P<0.01). Statistical analysis showed that CH4 flux was also correlated with soil water content and microbial biomass carbon but not with soil temperature. In the long run, small precipitation events (1-8 mm) could enhance the intensity of soil CH4 sink and such promoting effect would be weakened with further increase in precipitation amount. However, large precipitation events (≥16 mm) could change the soil function from CH4 sink to source by stimulating the activity of soil methanogens during short-term periods. [ABSTRACT FROM AUTHOR]

In order to reduce influence of underground environment on the positioning system,this paper presented a fingerprint positioning algorithm based on SVM classification. The algorithm consists of fingerprint database, underground tunnel fingerprint data acquisition and underground location matching. The SVM classification method was adopted to establish the fingerprint database, the singular value was removed to eliminate the effects of dynamic fingerprints, the best match position was found by matching the real-time sampling signal to the fingerprint database. This paper collected 50 samples by randomly fingerprint data as position information, muti-terminal user position information was measured and five user terminals measurement data were taken for analysis. The results of location experiments showed that the positioning error of proposed algorithm was less than 1.5 m, which was much better than traditional positioning algorithm based on RSSI. [ABSTRACT FROM AUTHOR]

In order to calculate the influence of high-order vibration modes for pushover analysis of tall buildings, based on modal pushover analysis (MPA) method, an equivalent elastic mode is established, which is applied to the transformation of freedom system from multiple degrees to single degree, and shear force form SRSS method is used as load pattern, thus the elastic-plastic displacement can be fast calculated by applying ductility demand spectra, inverse-SRSS distribution and SRSS combination, by which a simplified pushover analysis with influence of high-order vibration modes is put forward. Good agreement is achieved between the structural elastic-plastic displacement obtained by the proposed method and that obtained by MPA method. Therefore, for relatively regular structures, the elastic-plastic displacements under the maximum earthquake action may be quickly obtained by the proposed method in this paper. [ABSTRACT FROM AUTHOR]

AbstractObjective: To explore the distribution characteristics and clinical significance of peripheral blood monocyte subgroups in patients with diffuse large Bcell lymphoma(DLBCL)., Methods: The percentage of peripheral blood monocyte subsets of 82 DLBCL patients (including 32 newly diagnosis, 29 remission and 21 relapse) and 30 healthy controls were detected by flow cytometry, and the correlation with the clinical characteristics and its diagnostic value of DLBCL were analyzed., Results: The proportion of intermediate monocytes in patients with newly diagnosed DLBCL group was higher than that in healthy controls (t=5.888, P<0.01). The proportion in relapsed group was higher than those in newly diagnosed DLBCL group（t=2.106，P=0.04） and remission group （t=6.882， P＜0.01）, and the proportion of intermediate monocytes in newly diagnosed DLBCL group was higher than that in Remission group (t=3.969, P<0.01). With the increase of International Prognostic Index (IPI) score, the percentage of intermediate monocytes in patients with DLBCL increased (r=0.37). Furthermore, when the proportion of intermediate monocytes was 10.91% as the cutoff value, the sensitivity and specificity of the whole sample were 90.60% and 9100%, respectively., Conclusion: The disease progression is related to the increased intermediate monocytes, which can be used as a potential diagnostic index for DLBCL.

The agricultural non-point source pollution (ANPSP) load in Guangdong province is very large and has a serious impact on the regional ecological environment. Inventory analysis was used to assess and analyze the temporal and spatial variation characteristics of the ANPSP load of Guangdong province during 1999-2019, and the sources of ANPSP were discussed, as well as the relationship between them. The results showed that, during the past 20 years, the total ANPSP pollution loads of Guangdong province decreased by 6.08%, and the pollution loads of chemical oxygen demand (COD), total nitrogen (TN), and total phosphorus (TP) increased by -11.88%, 4.99%, and 26.17%, respectively. The input intensity of chemical fertilizers and pesticides increased by 112.19% and 60.38%, respectively. The Pearl River Delta had the highest ANPSP loads in Guangdong province, followed by those in northern, western, and eastern Guangdong. Livestock and poultry breeding were the main sources of COD, the total percent fertilizers and livestock and poultry breeding were the main sources of TN, and livestock and poultry breeding and aquaculture were the main sources of TP. In addition, the contribution of pollutants discharged from aquaculture showed an obvious increasing trend. There were certain differences in the pollution sources in different regions. In western Guangdong, northern Guangdong, and eastern Guangdong, livestock and poultry breeding were the main sources of COD and TP, and fertilizer was the main source of TN; by contrast in the Pearl River Delta, aquaculture had become the main source of TN and TP pollution loads. The correlation results showed that the decline in the total ANPSP in Guangdong province was mainly due to the increase in high urbanization rate and the decrease in the proportion of rural population. In general, there were stage changes in the time and differences in spatial characteristics and sources of ANPSP in Guangdong province. A combination of comprehensive treatment and targeted pollution treatment should be adopted, and fertilizer reduction measures and pollution treatment in the aquaculture should be strengthened in an all-around way, focusing on strengthening the treatment of pollution from aquaculture in the Pearl River Delta region and the treatment of rural life pollution in northern Guangdong.

To realize the comprehensive utilization of resources of moutai-flavor vinasse and improve the nitrogen efficiency of yellow soil, a field culture experiment was carried out to study the effects of short-term application of vinasse biochar on nitrogen availability and bacterial community structure diversity in yellow soil of Guizhou by setting 5 biochar dosages of 0% (MB 0 ), 0.5% (MB 0.5 ), 1.0% (MB 1.0 ), 2.0% (MB 2.0 ), and 4.0% (MB 4.0 ). The results showed that the total nitrogen(TN) and nitrate nitrogen(NN) content in the soil increased by 35.79%-365.26% and 122.96%-171.80%, the microbial biomass nitrogen (MBN) content decreased by 34.10%-59.95%, and the AN/TN, NN/TN, and MBN/TN exhibited a decreasing trend with an increase in the amount of biochar applied. The application of vinasse biochar significantly reduced the number of OTU and community richness and diversity of soil bacteria; the influence degree increased with an increase in the application amount of vinasse biochar. In comparison with the MB 0 treatment, the application of biochar significantly changed the soil bacterial community structure. The relative abundance of Bacteroidetes increased by 1.76-2.11 times with an increase in the biochar application. However, the relative abundance of Acidobacteria, Chloroflexi, Gemmatimonadetes, Planctomycetes, Armatimonadetes, Thaumarchaeota, and Nitrospirae decreased to different degrees, with the most significant decrease in the MB 4.0 treatment. The application of vinasse biochar increased the relative abundance of certain soil functional bacteria, such as Streptomyces and Pusillimonas , and simultaneously also decreased the relative abundance of the dominant bacteria, such as Lysobacter and Gemmatimonas . In addition, the redundancy analysis (RDA) showed that the MBN/TN, NN, and MBN were the main cause of soil bacterial community structure change in nitrogen environment factor. The MBN/TN and MBN exhibited a significantly positive correlation with Thaumarchaeota and Nitrospira , which indicated that the short-term application of vinasse biochar can significantly reduce the abundance of ammonia-oxidizing archaea and nitrifying bacteria, inhibit the ammonia-oxidizing effect and nitrification rate of soil, and improve the availability of soil nitrogen. In summary, the short-term application of vinasse biochar can improve nitrogen nutrients, change the structure and diversity of soil bacterial community, and effectively control the risk of soil nitrogen leaching by inhibiting ammonia oxidation and nitrification of soil, to improve the availability of soil nitrogen.

Purpose: To explore the differentially expressed genes between human mandible- and ilium- derived mesenchymal cells., Methods: Differentially expressed genes between the human mandible- and ilium- derived mesenchymal cells were obtained from the GEO database based on the analysis of GEO2R software, then the relationship among these genes were analysed using DAVID and Gene MANIA on-line database., Results: Two hundred and ninety three differentially expressed genes between the human mandible and iliac derived mesenchymal cells were obtained, and most of them were homeobox genes that related to bone development. These genes could also be enriched into different subgroups based on the analysis of DAVID database, such as "skeletal system development", "forebrain development", "limb development" and "WNT signaling pathway". After the analysis from Gene MANIA database, the molecular networks of MSX1, MSX2, HAND2, SIX1, PITX2, OSR2, PAX3 and PRRX1 were built to show that these eight genes exhibited interactions, including co-expression, co-localization, genetic interactions., Conclusions: There are different gene regulating mechanisms between the human mandible- and ilium- derived mesenchymal cells. It implies that genetic modification may be applied to get iliac derived mesenchymal cells to be more suitable for tissue-engineered repair of jaw.

Based on measurement on winter wheat soil respiration (SR), environmental and biotic factors from 2013 to 2014 in semi-humid and prone-to-drought area of Weihe Plain, this paper analyzed the characteristics of diurnal and seasonal variation of SR and influences of temperature (T), soil water content (W), gross primary productivity (GPP) and leaf area index (LAI). The results showed that the SR diurnal variation appeared as a single-peak curve ranging between 1.5 and 6.94 μmol CO 2 ·m -2 ·s -1 , with the peak presented during 12:00-14:00. Temperature was the driving factor for the SR diurnal variation, and land surface temperature (T s ) could explain 80.9% of the temporal variation for SR. The corresponding relationship between SR and temperature showed a clockwise nearly elliptic curve. From seedling stage to winter, the SR rate of wheat showed a descending trend, and stayed at a relatively low level until regreening stage. Then the rate of SR increased rapidly and reached peak in heading stage and filling stage, but there was a descending trend in mature stage. The SR values in growth period were between 0.65 and 4.85 μmol CO 2 ·m -2 ·s -1 . T, W, GPP and LAI were positively related to SR (P<0.01). Soil temperature and soil water content were the dominant factors affecting the SR seasonal variation, and they could explain 82.6% of its variation by a compound model SR=$e^{(a+bT&#95;{5 cm}+cW&#95;{10 cm}+dW&#95;{10 cm}^2)}$, which was much better than monovariant model (below 65.7%). Based on the compound model results, the average rate of wheat SR during growth period in 2013-2014 was 1.67 μmol CO 2 ·m -2 ·s -1 .

Objective: To observe the effect of electroacupuncture (EA) stimulation of "Zusanli" (ST 36), "Liangmen" (ST 21) and "Sanyinjiao" (SP 6) on gastrointestinal motility and expression of ghrelin and growth hormone secretagogue receptor (GHSR) mRNA in diabetic gastroparesis (diabetic gastroparesis, DGP) rats, so as to explore its mechanism underlying improvement of diabetic gastroparesis., Methods: Sixty SD rats were randomly and equally divided into normal control, model, EA acupoint, EA non-acupoint, medication (metoclopramide) groups (n = 12 rat/group). The DGP model was established by intraperitoneal injection of streptozotocin (STZ, 55 mg/kg, 2%) and raised by high-sugar and high-fat fodder irregularly for 8 weeks. EA was applied to "Zusanli" (ST 36), "Liangmen" (ST 21) and "Sanyinjiao" (SP 6), and non-acupoints (about 5 mm lateral to the 3 acupoints, respectively). Those rats of the medication group were given with metoclopramide (1 mL/100 g). The blood sugar level was measured by glucometer, and the urine sugar level measured by Tes-Tape [urine glucose test paper]. The intestinal propulsion rate (IPR) was identified as ratio of the phenolsulfonphthalein promoting distance and the total length of the small intestine, and the gastric emptying rate (GER) was assessed by gavage of a liquid test meal (1.5 mL) containing a nonabsorbable marker (0. 5 mg/mL of phenol red in 5% glucose solution) and identification after being sacrificed 72 h after modeling. The expression levels of ghrelin mRNA and GHSR mRNA of gastric antrum tissue were examined by Quantitative real-time PCR., Results: In comparison with the normal control group, the blood sugar and urine sugar levels of the model group was significantly increased in the model group (P < 0.01), while the GER and IPR and the expression level of ghrelin mRNA and GHSR mRNA in the gastric antrum were significantly decreased in the model group (P < 0.01, P < 0.05). Following EA stimulation of acupoint, the blood sugar and urine sugar levels were obviously down-regulated (P < 0.05), both GER and IPR, and ghrelin mRNA and GHSR mRNA expression levels in the EA-acupoint group were significantly up-regulated (P < 0.05, P < 0.01). No significant changes were found in both blood sugar and urine sugar levels, in both GER and IPR, and ghrelin mRNA and GHSR mRNA expression levels of both EA non-acupoint and medication groups after the intervention (P > 0.05). The GER and IPR were significantly higher in the EA acupoint group than in the EA non-acupoint group (P < 0.05, P < 0.01)., Conclusion: EA stimulation of ST 36, ST 21 and SP 6 can promote the gastrointestinal motility of DGP rats, which may be closely associated with its effects in up-regulating the expression level of ghrelin mRNA and GHSR mRNA in gastric antrum.

To understand the short-term response of soil CH4 flux to different precipitation events in a winter wheat field on the Loess Plateau, we conducted precipitation simulation experiments during winter wheat jointing stage and summer fallow period to measure the soil CH4 flux in 0 to 72 h after precipitation simulation of 1 to 32 mm. Results showed that CH4 flux during 0 to 72 h fluctuated at 1 to 8 mm of precipitation application and peaked at 16 and 32 mm. Cumulative CH4 flux after 72 h of precipitation (CH4-C) increased linearly with the precipitation amount (P) (wheat jointing stage: CH-C=2.45P-6.09, R2=0.92, P<0.01; summer fallow: CH-C=2.43P-4.73, R2 = 0.91, P<0.01). Statistical analysis showed that CH4 flux was also correlated with soil water content and microbial biomass carbon but not with soil temperature. In the long run, small precipitation events (1-8 mm) could enhance the intensity of soil CH4 sink and such promoting effect would be weakened with further increase in precipitation amount. However, large precipitation events (≥16 mm) could change the soil function from CH4 sink to source by stimulating the activity of soil methanogens during short-term periods.

This study was aimed to explore the pathogenesis of type III familial hemophagocytic lymphohistiocytosis (FHL3) via susceptibility gene UNC13D involving in homologous recombination repair (HRR) of DNA double-strand break (DSB). By means of DNA homologous recombination repair, the change of homologous recombination repair rate of normal control cells and DR-U2OS cells after down-regulation of UNC13D was detected; the UNC13D gene related function was explored. The results showed that DR-U2OS cells displayed a significant reduction in homologous recombination repair of DNA DSB after siRNA knockdown of UNC13D, compared to its normal control cell counterparts (P < 0.05), suggesting that UNC13D was involved in DNA double-stranded breakage repair. It is concluded that UNC13D gene mutation may be involved in the pathogenesis of FHL3 via its dual effects of both the cytotoxic granule exocytosis and decrease of homologous recombination repair rate after the DNA double-strand break, therefore, providing a new theoretical basis to reveal the pathogenesis of FHL3.

Objective: To investigate the underlying tumor susceptibility mechanisms and reasons for the high risk of cancer in Fanconi anemia (FA)., Methods: Gene Set Enrichment Analysis (GSEA) was performed to compare gene expression profiles between 21 FA patients' bone marrow (BM) mononuclear cell (BMNC) and 11 normal controls in cancer related gene sets from NCBI GEO database, then core enriched genes were identified by further investigation. Through enrichment analyzing biological processes of gene ontology sets and structural genomic gene sets between FA expression profiles and control, more details related with its tumor susceptibility had been revealed., Results: Compared with normal control, gene expression in FA group had significant been enriched in resistance to Bcl-2 inhibitor gene set, fibroblast growth factors signalling pathways, insulin and insulin-like growth factors (IGF) signalling pathways induced cancer genesis gene sets. The high level of D4S234E, SST, FGFs, IGFs, FGFRs and IGFBP expression provided an initiate environment for tumorgenesis and drug resistance. There were significant differences in biogenesis extracellular molecules and cytomembrane structure organizations between FA and control. Genes with promoter regions around transcription start sites containing either motif RRCAGGTGNCV or CCTNTMAGA were enriched and those former genes match annotation for tumorgenic transcription factor 3 (TCF3)., Conclusions: The high tumor susceptibility of FA patients may be closely related with the dramatic changes in cancer related growth factors and hormones environment. This study provides new insights into tumor susceptibility mechanism in FA patients.

Objective: To evaluate the effect of gemcitabine in enhancing the radiosensitivity of hepatoma cell line HepG2 and explore its mechanisms., Methods: Clonogenic survival assay is employed to calculate the ratios of L-Q model radiation biology parameters and radiosensitization after different doses of irradiation. Flow cytometry was used to detect the changes in HepG2 cell cycle and apoptosis rate after gemcitabine treatment and radiation exposure., Results: The survival fraction at 2 Gy of HepG2 cells treated with gemcitabine was significantly lower, and the value of alpha was significantly higher than those of untreated cells. GEM treatment increased the percentage of radiation-induced G0/G1 phase cells and cell apoptosis., Conclusion: Gemcitabine can significantly enhance the radiosensitivity of HepG2 cells by enhancing radiation-induced cell cycle arrest in G0/G1 phase and cell apoptosis.

Objective: To evaluate the effect of COX-2 silencing on the radiosensitivity of a nasopharyngeal carcinoma (NPC) cell line C666-1., Methods: Anti-COX-2 C666-1 cell line with COX-2 gene silencing mediated by shRNAmir lentiviral vector and the control cell line Anti-GL-2 C666-1 were exposed to various radiation doses. The clonogenic survival assay and curve fitting was used to calculate the radiobiological parameters and the sensitization enhancement ratio after the radiation. Cell cycle changes were assessed after the exposure by flow cytometric analysis. In a BALB/c nude mouse model, the growth curve of the xenografts was generated and the tumor growth inhibition rate was calculated., Results: Compared with the control cells, Anti-COX-2 C666-1 cells showed obviously lowered values of SF2, D0 and Dq but significantly increased α/β with a sensitivity enhancement ratio of 1.4014. COX-2 gene silencing increased the inhibition rate of the tumor xenografts after the radiation, and caused also decreased percentage of G2/M arrest resulting from the exposure., Conclusion: Stable COX-2 silencing in NPC cells can improve the effect of radiotherapy both in vitro and in vivo. By changing the radiobiological parameters, genetically based COX-2 inhibitor may be a potentially promising radiosensitizer of NPC.

Objective: To investigate radiation-induced cell cycle changes of human breast cancer stem cells enriched by suspension culture., Methods: The tumorigenicity of human breast cancer stem cell line MCF-7 cultured in serum-free media was confirmed in NOD/SCID mice, and the radiosensitivity of the cells was tested by clone formation assay following radiation exposure. Flow cytometry was performed to evaluate radiation-induced cell cycle changes, and the protein expression of pCDC25C (ser216) was measured by Western blotting., Results: After the exposure to 2 Gy radiation, the survived fraction of the cells in suspension culture and those in adherent culture was 0.856 ∓ 0.061 and 0.783 ∓ 0.097, respectively, and the cells in suspension culture showed an obviously greater capacity of tumorigenicity in NOD/SCID mice. The radiation exposure resulted in an obvious increase in the proportion of G2 phase cells from (22.03 ∓ 2.12)% to (45.83 ∓ 2.25)% and significantly increased the expression of pCDC25C (ser216)., Conclusion: Radiation- induced G2 phase arrest may contribute to the resistance of the breast cancer stem cells to radiotherapy.

The authors for the first time fabricated OLEDs employing novel phthalocynines: 2(3)-(p-tert-butylphenoxy) copper phthalocyanine(1), 2(3),16(17)-di(p-tert-butyl-phenoxy) copper phthalocyanine(2) and 2(3), 9(10), 16(17)-tri (p-tert-butylphenoxy) copper phthalocyanine(3) as light emitting layer, and their electroluminescence character was studied. The final structures of three-layer OLEDs based on copper 2(3)-(p-tert-butylphenoxy) copper phthalocyanine (1) and 2(3), 9(10), 16(17)-tri (p-tert-butylphenoxy) copper phthalocyanine(3) were ITO/NPB(40 nm)/Pc(30 nm)/AlQ(43.5 nm)/LiF (0.5 nm)/Al(120 nm). The structure of three-layer OLED based on 2(3), 9(10), 16(17)-tri (p-tert-butylphenoxy) copper phthalocyanine (3) was ITO/NPB(30 nm)/Pc(30 nm) /BCP(20 nm)/A1Q(30 nm)/LiF (0. 5 nm)/Al(120 nm). Room-temperature electroluminescence was observed at about 869 nmand 1 062 nm for 2(3)-(p-tert-butylphenoxy) copper phthalocyanine(1); room-temperature electroluminescence of 2(3),16(17) -di(p-tert-butyl-phenoxy) copper phthalocyanine(2) was found at about 1050 nm and 1110 nm; and room-temperature electroluminescence of 2(3), 9(10), 16( 17)-tri (p-tert-butylphenoxy) copper phthalocyanine(3) was studied at about 1095 and 1204 nm. The emission wavelengths and the half bandwidths were quite different for the phthalocyanine, which may be due to the differences in the number of substituted and the molecular aggregations in vacuum sublimed films. The difference in Stokes shift relaxation was also induced by the molecular aggregations.

Objective: To compare therapeutic effects of acupoint-injection combined with electroacupuncture and oral administration of medicine on chronic pharyngitis., Methods: The treatment group of 71 cases were treated by injection of fufangdanshen injectio into Tiantu (CV 22) combined with electroacupuncture at Renying (ST 9), and the control group of 51 cases were treated by oral administration of acetylspiramycin and qingyan tablet., Results: The cured rate and the total effective rate were 60.5% and 90.1% in the treatment group, and 21.6% and 68.6% in the control group, with significantly differences between the two groups (P < 0.01)., Conclusion: Therapeutic effect of acupoint-injection combined with electroacupuncture on chronic pharyngitis is superior to that of oral administration of medicine.