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Background As an objective index to evaluate chronic stress, allostatic load (AL) is associated with adverse pregnancy outcomes. At present, there are few longitudinal studies to analyze the influencing factors of AL among pregnant women. Objective To investigate the status of AL and its influencing factors in pregnant women at different trimesters. Methods This study was designed as a prospective study. Questionnaire survey, physical examination and laboratory examination were administered to 152 women in the first (≤14 weeks), second (23-27 weeks) and third (30-34 weeks) trimesters of pregnancy collected in the obstetrics outpatient clinics of the 901 Hospital, Joint Logistic Support Force of the Chinese People's Liberation Army, Jin'an Maternal and Child Health Care Hospital from November 2021 to November 2022 by using convenience sampling method. The Edinburgh Postpartum Depression Scale (EPDS) was used to assess the depression during pregnancy. Referring to previous studies, the relevant assessment indexes of metabolic system, cardiovascular system, and immune system were used to calculate the total score of AL, and AL≥3 score was used as a criterion for determining high AL level at different trimesters of pregnancy. Multivariate Logistic regression analysis was used to explore the influencing factors of AL in the first, second and third trimesters. Results Among 152 pregnant women, the mean total AL score was (2.06±1.68), (2.07±1.84) and (2.07±1.68) in the first, second and third trimesters; 52 (34.2%), 54 (35.5%) and 50 (32.9%) women were in high level of AL (total score of AL≥3) in the first, second and third trimesters. The results of multivariate Logistic regression analysis showed that occupations {business service employees〔OR=0.229, 95%CI (0.062, 0.845), P=0.027〕, office staff〔OR=0.164, 95%CI (0.051, 0.528), P=0.002〕, professional and technical personnel〔OR=0.278, 95%CI (0.099, 0.784), P=0.015〕}, unemployment〔OR=5.516, 95%CI (1.044, 29.144), P=0.044〕and depression〔OR=6.241, 95%CI (1.403, 27.757), P=0.016〕were the influencing factors of AL in the first trimester. Age〔OR=1.098, 95%CI (1.002, 1.202), P=0.045〕and AL in the first trimester〔OR=9.965, 95%CI (4.402, 22.561), P

This study aimed at the epitope mapping and allergencity reduction of the wheat non-gluten allergen α-amylase inhibitor. Its linear epitopes were predicted by using bioinformatic prediction tools DNAstar, IMED, and IEDB. Six candidate epitopes were obtained by comprehensive analysis, and four linear epitopes were verified by using indirect competition enzyme-linked immunosorbent assay (ic-ELISA). The allergenicity of the allergen was reduced by interaction with polyphenols, A non-covalent complex of α-amylase inhibitor and epigallocatechin gallate (EGCG) was prepared and characterized by fluorescence spectroscopy, circular dichroism (CD) spectroscopy and ultraviolet (UV) spectroscopy, and the efficiency of allergenicity reduction was investigated by ic-ELISA. Results showed that the complex with a molar ratio of α-amylase inhibitor to EGCG of 1:30 had the lowest IgE-binding capacity. The binding capacity was reduced by 22.7%. Molecular docking simulations by using Autodock software further demonstrated that the allergenicity reduction was due to the interactions of the linear epitopes of α-amylase inhibitor and their nearby residues with EGCG. This study can provide a reference for the epitope mapping of wheat non-gluten allergens and the preparation of polyphenols to reduce their allergenicity.