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Lnc-HUR1 is an HBV-related long non-coding RNA, which can promote the proliferation of hepatoma cells and the occurrence and development of liver cancer. In this study we explored the effect of lnc-HUR1 on the apoptosis of hepatocellular carcinoma cells by taking the approach of immunoblotting, quantitative real time PCR, luciferase reporter assay, chromatin immunoprecipitation (ChIP) and flow cytometry. We found that overexpression of lnc-HUR1 significantly reduced the activity of caspase3/7 and the cleavage of PARP-1, while knocking down of lnc-HUR1 significantly increased the activity of caspase3/7 and promoted the cleavage of PARP-1 in HepG2 cells treated with TGF-β, pentafluorouracil or staurosporine. Consistently, the data from Annexin-V/PI staining showed that overexpression of lnc-HUR1 inhibited apoptosis, while knockdown of lnc-HUR1 promoted apoptosis. Moreover, overexpression of lnc-HUR1 up-regulated the apoptosis inhibitor Bcl-2 and down-regulated the pro-apoptotic factor BAX at both RNA and protein levels. In the CCL4-induced acute liver injury mice model, the expression of Bcl-2 in the liver tissue of lnc-HUR1 transgenic mice was higher than that of the control mice. The data from ChIP assay indicated that lnc-HUR1 reduced the enrichment of p53 on Bcl-2 and BAX promoters. All these results indicated that lnc-HUR1 inhibited the apoptosis by promoting the expression of apoptosis inhibitor Bcl-2 and inhibiting the expression of apoptosis promoting factor BAX. Further studies showed that lnc-HUR1 regulated the transcription of Bcl-2 and BAX in HCT116 cells, but had no effect on the expression of Bcl-2 and BAX in HCT116 p53 -/- cells, indicating that lnc-HUR1 regulates the transcription of Bcl-2 and BAX dependent upon the activity of p53. In conclusion, HBV upregulated lnc-HUR1 can inhibit the apoptosis of hepatoma cells. Lnc-HUR1 inhibits apoptosis by inhibiting the transcriptional activity of p53. These results suggest that lnc-HUR1 plays an important role in the occurrence and development of HBV-related hepatocellular carcinoma.

A two-year field experiment was carried out in a Cu-Zn-Cd-Pb-contaminated field with Sasa auricoma, Indocalamus tessellatus , and Shibataea chinensis Nakai to investigate their growth and accumulation characteristics. Based on changes in heavy metal content in the soil, we compared their phytoremediation potentials for the combined pollution of heavy metals. After two years of planting, S. auricoma showed the best tolerance to soil contaminated by heavy metals, with the number of plants being 63.8 times that at the time of planting. For each bamboo type, heavy metals were most concentrated in the root, with the best enrichment ability of Cd and worst ability of Pb. The bioconcentration factor of Cd in the roots was far greater than 1 for all three species, with 17.68 for S. auricoma and 14.63 for I. tessellatus . The accumulation of heavy metals was mainly concentrated in the roots and rhizomes, and the accumulations of Cu, Zn, and Cd in S. auricoma were 157.14, 363.3, and 7.18 g·hm -2 , respectively, which were higher than those of I. tessellatus and S. chinensis Nakai . Compared with that before planting, the content of heavy metals in the non-rhizosphere soil decreased after two years of planting, among which Cd content decreased the most, from 39.6%-40.4%. In addition, the contents of Cu, Zn, and Cd in rhizosphere soil of S. auricoma and S. chinensis Nakai were significantly lower than those in non-rhizosphere soil ( P <0.05). The results showed that the three species had strong accumulation ability of Cd and could be further studied as Cd-repaired plants. The growth condition and content and accumulation of heavy metals in S. auricoma were excellent among the dwarf bamboos, which showed the highest phytoremediation potential.

Objective: To compare the efficacy and safety of pro-urokinase and reteplase in the treatment of patients with acute ST elevation myocardial infarction (STEMI). Methods: STEMI patients, who received intravenous thrombolytic therapy in Henan STEMI registry between September 2016 and August 2018, were eligible for this study. A total of 5479 patients from 66 hospitals were screened and patients were divided into pro-urokinase group ( n =638) and reteplase group ( n =702) according to thrombolytic drugs. Data including patient demographics, risk factors, medical histories, patient information at admission, in-hospital treatment, time delays, and clinical events were collected. The clinical recanalization rate, in-hospital mortality, in-hospital death or treatment withdrawal, in-hospital main adverse cardiovascular and cerebrovascular events (MACCE, death or treatment withdrawal, congestive heart failure, reinfarction and ischemic stroke) and post-thrombolysis bleeding were compared between the two groups. Bleeding events were evaluated with Bleeding Academic Research Consortium (BARC) criteria. Results: The median age [61.8 (53.2, 69.0) vs. 62.6 (52.1, 69.8), P =0.833] or the proportion of women [23.0% (147/638) vs. 25.1% (176/702), P =0.385] were similar between the pro-urokinase and reteplase groups. Clinical recanalization rates were similar between the pro-urokinase and reteplase groups [82.1% (524/638) vs. 84.9% (596/702), P =0.172], and there was no difference in the median time from onset to thrombolysis [194.5 (135.0,290.0) min vs. 190 (126.0,292.0) min, P =0.431] and the median recanalization time [95 (67.5,120.0) min vs. 95 (71.0,119.0) min, P =0.561] between the two groups. There was no significant difference in in-hospital mortality [5.5% (35/638) vs. 5.1% (36/702), P =0.770], in-hospital all-cause mortality, treatment withdrawal [8.9% (57/638) vs.7.7% (54/702), P =0.410], and in-hospital MACCE [13.0% (83/638) vs. 10.4% (73/702), P =0.137] between pro-urokinase and reteplase groups. However, the incidence of post-thrombolysis bleeding was significantly higher in reteplase group than in pro-urokinase group [7.8% (55/702) vs. 3.8% (24/638), P =0.002]. Further analysis found that the incidence of oral bleeding and the BARC grades 1-2 bleeding were significantly higher in reteplase group than in pro-urokinase group, whereas the incidence of cerebral hemorrhage was similar between the two groups [0.6% (4/638) vs. 0.4% (3/702), P =0.715]. The comparison of efficacy and safety outcomes between the two groups after adjusting for baseline characteristics using general linear mixed models was consistent with those before the adjustment. There was no significant difference in in-hospital mortality, in-hospital death or treatment withdrawal, in-hospital MACCE after adjusting for baseline characteristics and post-thrombolysis bleeding between the two groups. Conclusions: Pro-urokinase and reteplase have similar clinical efficacy in the treatment of STEMI. In terms of safety, the incidence of cerebral hemorrhage is similar, while the incidence of BARC grades 1-2 bleeding and oral bleeding is higher in reteplase group than in pro-urokinase group, which has no impact on in-hospital outcomes.

Pyrus xerophila has strong resistance to drought, cold, salt, and rust. It is one of the main rootstock types of pear in Northwest China, with high production value. However, its habitat is seriously damaged. The prediction of the geographical distribution of P. xerophila under different climate scenarios will provide important scientific basis for rational development and utilization of resources and the protection of diversity. Based on MaxEnt model and ArcGIS spatial analysis, the potential spatial distribution pattern of P. xerophila in the current and future (2050, 2070) was constructed using comprehensive and accurate distribution records and high-resolution environmental data, and the relative importance of environmental factors was evaluated. The results showed that the suitable habitat area of P. xerophila was 3.32×10 5 km 2 , mainly located in eastern Qinghai, southern Gansu, southern Ningxia, central Shaanxi, southern Shanxi and western Henan. Mean UV-B of the month with lowest UV-B and altitude were identified as the critical factors shaping habitat availability for P. xerophila. Overall, with global warming, the potential habitat for P. xerophila might decrease in 2050 and 2070 under different CO 2 climate scenarios. Real-time monitoring of P. xerophila populations should be enhanced.

To study the seasonal pollution characteristics and sources of water-soluble inorganic ions in atmospheric PM 2.5 in Suqian City, 171 samples were collected at three monitoring points, which were in the water vapor channel, from May 2017 to January 2018. The mass concentrations of PM 2.5 and nine water-soluble inorganic ions were analyzed. The results showed that the annual average concentration of water-soluble inorganic ions in PM 2.5 in Suqian City was (44.08±34.61) μg ·m -3 , accounting for 41.8% of PM 2.5 . The concentrations of these species were in the order of ρ (NO 3 - ) > ρ (SO 4 2- ) > ρ (NH 4 + ) > ρ (ρl - ) > ρ (Na + ) > ρ (Ca 2+ ) > ρ (K + ) > ρ (F - ) > ρ (Mg 2+ ); NO 3 - , SO 4 2- , and NH 4 + accounted for 75.6% of the total water-soluble ions. The annual average ratio of ρ (NO 3 - ) to ρ (SO 4 2- ) was 1.53±0.88, indicating that mobile sources contributed more to PM 2.5 pollution. Based on the correlation analysis of NH 4 + and SO 4 2- , NO 3 - may exist in the form of (NH 4 ) 2 SO 4 , NH 4 HSO 4 , or NH 4 NO 3 . According to the principal component analysis, secondary transformation, industrial pollution, biomass burning, and dust were the major sources of water-soluble inorganic ions. PM 2.5 concentrations were positively related to relative humidity in winter. Water vapor transmission is more likely to promote PM 2.5 accumulation in winter.

Objective: To explore the differential protein expressions in papillary thyroid carcinoma (PTC) with or without Hashimoto's thyroiditis (HT). Methods: Tissue microarray was prepared and the protein expression levels of v-Raf murine sarcoma viral oncogene homolog B1 (BRAF), vascular endothelial growth factor (VEGF), cyclinD1, mesothelial cell (MC) , CD56 and Galectin3 in the PTC tissues with or without HT were detected by immunohistochemical staining. Results: The positive expression rates of BRAF protein in the PTC tissues with or without HT groups were 55.4% (36/65) and 63.6% (42/66), respectively, without significant difference ( P =0.336). The positive expression rates of VEGF protein in the PTC tissues with or without HT groups were 25.7% (19/74) and 25.8%(17/66), respectively, without significant difference ( P =0.991). The positive expression rates of cyclin D1 protein in the PTC tissues with or without HT groups were 93.4% (71/76) and 97.6% (80/82), without significant difference ( P =0.206). The positive expression rates of MC protein in the PTC tissues with or without HT groups were 86.1% (62/72) and 83.5%(71/85), without significant difference ( P =0.654). The positive expression rates of Galectin3 protein in the PTC tissues with or without HT groups were 98.7% (76/77) and 97.5% (78/80), without significant difference ( P =0.583). The positive expression rates of CD56 in the PTC tissues and adjacent thyroid follicular epithelial cells were 27.4% (32/117) and 65.0% (76/117), respectively, and the difference was statistically significant ( P =0.001). The positive expression rates of CD56 in PTC tissues with or without HT were 35.5% (24/68) and 16.5% (13/79), respectively, and the difference was statistically significant ( P =0.009). Conclusions: There are no significant differences in the expressions of BRAF, VEGF, CyclinD1, MC and Galectin3 between the PTC tissues with or without HT. However, the significantly differential expression of CD56 between the two group suggests that CD56 may be related to the pathogenesis of PTC with HT. CD56 may be used as a potential molecular marker in PTC diagnosis.

Background: Lung cancer is one of the most dangerous diseases to human health, with high morbidity and mortality. It can be cured by surgery at early stage, therefore, the early detection and early treatment of lung cancer are especially important. Serum tumor markers play an important role in the detection and diagnosis of lung cancer. Galectin-3 is known to be expressed in a variety of malignant tumors. This study was to explore the serum levels of Galectin-3 and its clinical significance in non-small cell lung cancer (NSCLC) patients., Methods: The serum levels of Galectin-3 in peripheral blood were detected by enzyme linked immunosorbent assay (ELISA) in 69 NSCLC patients and 77 cases of healthy control subjects, and compared between the two groups. Then we analyze the correlations between the serum levels of Galectin-3 and the clinical features of lung cancer., Results: The serum levels of Galectin-3 in NSCLC patients were significantly higher than those of healthy control subjects (P<0.01). The serum levels of Galectin-3 with lymph node metastasis were significantly higher than those of patients without lymph node metastasis (P<0.01), and N2 lymph node metastasis had higher levels of serum Galectin-3 than those of N1 lymph node metastasis (P<0.01). Clinical stage III and stage IV patients had higher levels of serum Galectin-3 than those of clinical stage I and clinical stage II (P<0.05)., Conclusions: Our study showed the serum levels of Galectin-3 are highly expressed in NSCLC patients and are significantly related to lymph node metastasis. It may be a potential tumor marker for lung cancer.

Objective: To understand the dietary intake quantity and quality of high school students in Shanghai education., Methods: From 2015 to 2016, Shanghai center for Disease Control and Prevention, divided the school into three levels(urban area, suburban area and rural area) according to the economic level and population composition of the street where the school was located. The probability-proportional-to-size sampling technique was used to select 19 high schools, from which 9 boys and 9 girls from same class were randomly recruited for each grade. A total of 1026 students were recruited. The survey included general situation questionnaire and dietary inquiry. Participants were required to complete the standardized questionnaire form through face-to-face interviews with trained interviewers. Chinese Dietary Guidelines(2016) was used to evaluate dietary quantity while China healthy diet index(CHDI) was used to evaluate dietary quality., Results: The medians of intake of grain, vegetables, fruits, meat/livestock, fish/shrimp, eggs, milk and milk products, bean and bean products, cooking oil, cooking salt were 313. 8, 189. 7, 66. 3, 179. 2, 34. 7, 48. 9, 133. 3, 33. 3, 27. 1 and 6. 0 g/d within high school students, respectively. In both boys and girls, the medians of intake of vegetables, fruits, fish/shrimp, dairy products were lower than reference standard and meat/livestock was higher(P<0. 05). The intake differences among different area's high school students upon grain, vegetables, fruits, poultry/livestock, fish/shrimp, dairy products, beans/nuts, cooking oil, cooking salt were statistically significant(P<0. 05). The CHDI median total score of high school students was 68. 5 in Shanghai. 75. 2% of the CHDI total scores were between 60 and 80. The vegetable score, dark vegetable score, fruit score and total CHDI score in boys were significantly lower than those in girls(P<0. 05). The urban area CHDI score was significantly higher than suburban's and countryside's(P<0. 05)., Conclusion: Both dietary structure and dietary quality are inappropriate and unfit within high school students in Shanghai, the urban area's condition is better than another two. We suggest to increase intake of vegetables, fruits, fish/shrimp, dairy products while decrease poultry/livestock's consumption.

To resovle the problems of in-situ conservation and resource utilization of Pyrus caller-yana, maximum entropy model (MaxEnt) and geographic information system (GIS) were used to predict the global ecological suitable region of P. calleryana in different climate scenarios based on 236 distribution data and 19 ecological factors. The results showed that the ecological suitable regions of P. calleryana were mainly concentrated in North America, Asia and other regions, with a total area of 1.6×10 7 km 2 . In China, the regions with high ecological suitability were Hunan, Hubei, Anhui, Jiangxi, Jiangsu, Zhejiang and Fujian provinces. The main factors affecting the geographical distribution of P. calleryana were mean annual temperature and mean annual precipitation, followed by the seasonality of temperature. The model simulations indicated that P. calleryana would have different suitable habitat areas and marginally suitable habitat areas in different climate scenarios. In terms of the spatial distribution of the potential habitat area, both the distributional range and the center of distribution of suitable and marginally suitable habitat area would shift from east to west. The suitable habitat area in North America and marginally suitable habitat areas in Europe would increase rapidly.

Objective: To investigate the clinicopathological and immunohistochemistrical features of secretory carcinoma of the breast (SCB), as well as its diagnosis and prognosis. Methods: Five cases of SCB were collected and specimens were fully drawn. Microscopic pathology examinations and immunohistochemistry(SP method)were performed. Results: Five cases were all female patients aged from 31 to 54 years old (with an average age of 38 years), and all patients initially presented with the painless breast lump, often near the areola. Macroscopically, all the tumors were relatively circumscribed, with a mean diameter of 2.1 cm(1.2-3.0 cm). Histologically, the tumors revealed different patterns (microcystic, solid, ductal, and so forth) and appeared large amounts of intracellular and extracellular eosinophilic PAS- and AB-PAS- positive secretory materials, as well as the granular eosinophilic cytoplasm. Immunohistochemistrically, the tumor cells were strongly positive for epithelial membrane antigen (EMA) and S-100. Three cases had negative expression for estrogen receptor (ER) and progesterone (PR), and two cases had weakly focal expression. Human epidermal growth factor receptor 2 (HER2) and P53 were negative, while only one case had weakly focal expression. The tumors were positive for CK7, CK5/6, CD10, but they were negative for CEA, smooth muscle actin (SMA) and P63 . The proliferation index of Ki-67 was 5%-8%. There was no patient with metastasis of the tumor in axillary lymph node. Except 1 case lost follow-up, and the rest had no recurrence or distant metastasis. Conclusions: SCB is very rare, with low malignancy, and has better prognosis, but it is usually classified as triple-negative breast cancer because of the immune phenotype. The correct diagnosis of this lesion depends on clinical characteristics, morphology and immunohistochemistry.

Objective: To investigate the effects of Vaspin on lipopolysaccharide (LPS)-induced acute respiratory distress syndrome (ARDS) in mice and explore the possible mechanism., Methods: Forty male C57B/L6 mice were randomized equally into control group, LPS group, Vaspin group and wortmannin group with corresponding treatments. The pathological changes of the lung tissues were evaluated by HE staining, and the severity of pulmonary edema was measured according to the wet/dry ratio (W/D) of the lung tissue. The lung permeability was evaluated by detecting total protein concentrations in the bronchoalveolar lavage fluid (BALF) using bicinchoninic acid (BCA) assay. Myeloperoxidase (MPO) activity in the lung tissue was detected using a MPO assay kit, and the levels of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in the lungs were measured using ELISA. Immunohistochemical staining was performed to detect the expression of vascular cell adhesion molecule-1 (VCAM-1) and Western blotting was used to detect the protein expressions of cleaved caspase-3 and p-Akt in the lung tissues., Results: Compared with the control group, the mice in LPS group displayed typical ARDS pathological changes in the lungs with significantly increased W/D, total protein concentrations in BALF, lung MPO activity, levels of IL-1β and TNF-α, and pulmonary expressions of VCAM-1 and cleaved caspase-3 (P<0.05) but decreased expression of p-Akt (P<0.05). These changes induced by LPS were significantly alleviated by the administration of Vaspin (P<0.05). The protective effects of Vaspin against ARDS were obviously attenuated by the PI3K inhibitor wortmannin (P<0.05)., Conclusion: Vaspin protects against LPS-induced ARDS in mice possibly by inhibiting inflammation and protecting vascular endothelium through upregulation of the PI3K/Akt signal pathway.

Objective: To evaluate the efficacy and safety of intravenous thrombolytic therapy using reteplase in patients with acute ST-segment elevation myocardial infarction (STEMI). Method: A total of 73 hospitals from Henan province took part in this clinical trials during October 2012 to October 2014, 1 226 cases (1 014 male (82.7%), mean age 59.0 (51.0, 66.0) years) with acute STEMI received reteplase as thrombolytic agent.Reperfusion rate was judged according to the clinical symptoms, electrocardiogram, myocardial enzymes and heart rhythm, and the rate of cardiovascular events and bleeding events during hospitalization was also observed.Bleeding events were evaluated with global utilization of streptokinase and tissues plasminogen activator for occluded coronary arteries (GUSTO) criteria.Subgroup analysis was performed to compare the effects of various thrombolysis timing (time from onset to thrombolysis≤6 h or 6-12 h) on reperfusion rate, cardiovascular events and bleeding events rate. Results: The reperfusion rate was 89.3% (1 089/1 219) at 120 minutes after the thrombolysis, average recanalization time was (59.96±26.86) minutes.The reperfusion rate of ≤6 h thrombolysis group was significantly higher than in 6-12 hours group (90.3% (988/1094) vs. 80.8% (101/125), P =0.001), while in-hospital mortality (2.6%(28/1 094) and 0.8% (1/125), P =0.352) and rate of bleeding (5.9%(64/1 094) and 5.6%(7/125), P =0.910) were similar between the two groups. The total in-hospital mortality after thrombolysis was 2.4% (29/1219), which was significantly higher in failed recanalization group than in recanalization group (10.8%(14/130) vs. 1.4%(15/1089), P < 0.001). The total rate of bleeding after thrombolysis was 5.8% (71/1219), there were 3 severe bleeding cases according to GUSTO classification (0.2%), all of them were cerebral hemorrhage, and 2 out of 3 cases died. Conclusions: Reteplase use is related to high recanalization rate and low cardiovascular events and bleeding rate and our results thus show that reteplase is a safe and effective thrombolytic agent for STEMI patients.

Interleukin(IL)-35 is a new member of the interleukin-12 superfamily. Since its first report in 2007, IL-35 rapidly became a research highlight in the field of immunology. Like other IL-12 superfamily members, IL-35 was a heterodimer which was composed of an α chain P35 and a β chain Epstein-Barr virus induced gene 3 (EBI3). Recent research work revealed two distinct roles of IL-35. Firstly, IL-35 is highly expressed in some kinds of inflammatory diseases and autoimmune diseases and plays import roles in the pathogenesis. Secondly, IL-35 is positively expressed in some cancers and plays some roles in the process of tumor progression. Here we demonstrate the structure and the signalling of IL-35. We reviewed the the roles of IL-35 in promoting tumor progression.

Qi arrival is the meridian qi response to acupuncture stimulation. Through analyzing the relevant concepts of qi arrival and summarizing the general understanding of it in clinic and on the basis of the collection of the relevant literature at home and abroad on the determination of qi arrival and its strength, the characteristics are analyzed on the present method and the method for the determination of qi arrival and its strength is discussed in terms of the results in the needling sensation scale. It is believed that the needling sensation and its strength can be used to determine whether the qi is arrived or not and its strength. The components of different types of needling sensation are much better applicable for the analysis on the characteristics and rules on the influence on qi arrival. This method is in compliance not only with the theoretic connotation of qi arrival, but also with the clinical general understanding, which lays the foundation for the analysis on the scale results.

From the time of Neijing (Internal Classic) and Nanjing (Classic of Medical Problems) till the end of Qing dynasty, the medical scholars of each dynasty had taken the recognition of deqi as the subject in the stud- ies. Through the historical analysis, the content of regulating deqi was further understood in relevant ancient liter- ature. By checking the ancient works of acupuncture in each dynasty till the end of Qing dynasty, in reference to the evidences in over 10 works, such as Zhenjiu Dacheng (Great Compendium of Acupuncture and Moxibustion) , Zhenjing Zhinan (Instruction of Acupuncture Canon), Zhenjiu Daquan, and in association with the academic views of acupuncture masters in modern time, it was discovered that the medical scholars after the time of Neijing and Nanjing had enriched the understandings of deqi, such as the connection of cold and heat reaction, radiation to the affected site, reinforcing and reducing purpose in qi regulation. The methods of deqi regulations had, been explored till the end of Qing dynasty since the time of Neijing and Nanjing and the understandings of it were vari- ous among scholars.

The wild Ussurian pear was the most important wild pear resource in northern China, belonging to the most hardiness species. Taking 48 accessions of wild Ussurian pear and 2 accessions of cultivated Ussurian pear as test materials, this paper studied the morphology of leaves, chlorophyll content, and photosynthetic characteristics. We compared the difference between the wild and cultivated Ussurian pear, analyzed the photosynthetic characteristics of wild Ussurian pear, clarified the interrelationships between the indices, and established significant linear equations of photosynthesis and water-related physiological indices. The results showed that the leaf morphological index, chlorophyll content and photosynthetic indices for cultivated Ussurian pear were significantly lower than their average values for wild Ussurian pear. The specific leaf area (SLA), leaf dry matter content ( LDMC) , and intercellular CO2 concentration had low coefficients of variation, and the other 8 indices had variation coefficient indices between 0.12-0.41. So, the level of diversity was high, indicating obvious difference in photosynthetic characteristic of wild pear resources in China. The photosynthetic indices were significantly correlated with chlorophyll composition (Chl a/b) and LDMC. The photosynthetic rate had significant exponential correlation with the intercellular CO2 concentration, transpiration rate, and stomatal conductance. The photosynthetic rate was mainly affected by stomatal limitation. The wild variety 'Jinzhoushanli' was selected as high photosynthetic genetic resource.

Formation of dermal collagen fiber is a complicated and sequential process with the progressive assembly of collagen. Collagen monomers form stepped and orderly protofibrils through longitudinal displacement. Subsequently, protofibrils or protofibrils and collagen are bonded by covalent bonds to form orderly lamellar structure of collagen fibers. Then collagen fibers are tightly wound into coarse collagen fiber bundles by covalent crosslinking. Decorin is a multifunctional small leucine-rich proteoglycan. It can prevent the aggregation of protofibrils by binding to the specific site of collagen with its core protein, and adjusting the spacing between the protofibrils with its glycosaminoglycan chain. Thus, by effecting the formation of collagen fibers with regulation of collagen assembly, decorin may help prevent scar formation and even promote regeneration.

Objective: To survey the association of serum free triiodothyronine (FT3) level with coronary artery calcification and major adverse cardiac events (MACE) in outpatients with suspected coronary artery disease (CAD)., Methods: A total of 577 outpatients with suspected CAD, who underwent dual-source computed tomography and FT3 detection were included, patients were followed up for 8-29 months for the major adverse cardiac events (death, MI, PCI, CABG). These patients were divided into low FT3 ( < 3.5 pmol/L, n = 126) and normal FT3 ( ≥ 3.5 pmol/L, n = 451) group based on the FT3 level, and divided into CACS > 100 (n = 235) and CACS ≤ 100 (n = 342) group based on the coronary artery calcium score (CACS). Related factors to CACS and MACE were analyzed using logistic regression (stepwise) analysis., Results: CACS (146.7 (55.8, 599.1) vs. 34.8 (0, 261.9), P < 0.001) and MACE (7.9% (10/126) vs. 2.0% (9/451) , P = 0.003) were significantly higher in the low FT3 group than in normal FT3 group. Logistic regression analysis demonstrated that the FT3 levels are inversely associated with the CACS (OR = 0.442, 95%CI = 0.317-0.618, P < 0.001). Kaplan-Meier analysis displayed that patients with low FT3 levels had a lower cumulative survival rate than patients with normal FT3 levels (P = 0.005), and patients with CACS > 100 also had a lower cumulative survival rate than patients with CACS ≤ 100(P < 0.001)., Conclusions: FT3 levels are associated with coronary artery calcification scores and the incidence rate of MACE in patients with suspected coronary artery disease. A low FT3 level is considered as an important risk factor of high calcification scores and MACE.

Objective: To investigate the protective effect of rutin against acute lung injury induced by lipopolysaccharide (LPS)., Methods: Thirty C57BL/6 mice were randomly divided into control group, LPS-induced acute lung injury model group and treatment (LPS+Rutin) group. The pathological changes of the lung tissue were observed microscopically on paraffin sections with HE staining, and the lung wet/dry weight ratio was measured. The levels of TNF-α and IL-1β in the bronchoalveolar lavage fluid (BALF) were measured with ELISA, and the expressions of α-ENaC were detected with RT-PCR and Western blotting., Results: Pathological examination of the lung tissue revealed distinct inflammation, congestion and edema in the model group. The mice in the treatment group showed significantly milder lung injuries than those in the model group. Compared with the control group, the model group showed significantly increased lung wet/dry ratio and contents of TNF-α and IL-1β in BALF but lowered expressions of α-ENaC mRNA and protein. Compared with the model group, rutin treatment significantly decreased the lung wet/dry ratio and TNF-α and IL-1β levels in the BALF and increased the expressions of α-ENaC mRNA and protein., Conclusion: Rutin can inhibit the pulmonary inflammation and increase the expression of alveolar epithelial sodium channel protein to alleviate LPS-induced acute lung injury in mice.

Objective: To investigate the changes of peroxisome proliferator- activated receptor-γ coactivator -1α (PGC-1α) mRNA and cytoapoptosis in the rats' masseter muscle which had been influenced by unilateral chewing, and to explore the theoretical foundation of changes in masticatory muscles induced by unilateral chewing., Methods: The animal models were established by extracting the Wistar rats' left maxillary molars. Thirty- six female Wistar rats were randomly divided into four groups of 2, 4, 6 and 8 weeks, nine each. In each group there were six rats with molar extracted and three as control. The Ca²⁺ level was detected by atomic spectrophotometric method. The relative expression of PGC-1α mRNA was detected by real- time fluorescent quantitative PCR. The apoptosis index was detected by Hoechst staining., Results: The Ca²⁺ level in the muscle on the extraction side were significantly higher than that in the controls in the beginning stage of unilateral chewing, and reached the peak at the 4th week [(43.62 ± 2.36) µg/g]. The relative expressions of PGC-1α increased from the beginning and reached the maximum level at the 4th week [extraction side: (1.57 ± 0.10); non-extraction side: (1.92 ± 0.06)], while the relative expressions of PGC-1α in 6 and 8 weeks decreased gradually [extraction side: (1.06 ± 0.08), (1.08 ± 0.07); non- extraction side: (1.09 ± 0.10), (1.11 ± 0.08)]. The changes of apoptosis index on non- extraction side increased continually and peaked at the 6th week [(38.56 ± 1.64)%]., Conclusions: PGC-1α and cytoapoptosis played important roles in different stages of tissue remodeling induced by unilateral chewing.

Objective: To analyze the risk factors for deep vein thrombosis (DVT) of the lower extremity in patients with bone metastases., Methods: Ninety patients with bone metastases were admitted to our hospital From January 2010 to December 2011, and their clinical data were retrospectively analyzed. There were 57 males and 33 females with a mean age of 61 years (range, 27 to 78 years). On admission, all cases were detected by color Doppler ultrasonography for DVT of bilateral lower extremities. Univariate and multivariate analyses were performed to determine the probable risk factors including gender, age, body weight, tumor location, bed confinement and etc., Results: Among the 90 patients, DVT was found in 24 patients on admission and the DVT incidence was 26.7% (24/90). The univariate analysis showed that bed confinement, multiple metastasis, pathological fracture, primary lesion detected, blood group, fibrinogen and hematocrit were significantly related to the incidence of DVT (P < 0.05). The logistic multivariate regression analysis showed that bed confinement, pathological fracture and fibrinogen were independent risk factors for the incidence of DVT., Conclusions: Bed confinement, pathological fracture and fibrinogen are independent risk factors for the incidence of DVT for patients with bone metastases. Patients with bed confinement >3 days, pathological fracture or fibrinogen >4 g/L should be routinely screened for lower extremity DVT on admission. Once identified, the DVT patients should be treated as early as possible.

Objective: This study aimed to investigate the effects of different tooth preparations on the fracture strength and pattern of failure of teeth with severe wedge-shaped defect restored with post and core crowns., Methods: According to whether the teeth above the wedge-shaped defect was removed (represented by B) or not (represented by A), the ferrule next to the wedge-shaped defect was prepared (represented by D) or not (represented by C), the cast post-and-core was chosen (represented by E) or glass-fiber post and resin core was chosen (represented by F). A total of 64 human mandibular premolar teeth were randomly divided into 8 groups: A1-1 (A + C + E), A1-2 (A + C + F), A2-1 (A + D + E), A2-2 (A + D + F), B1-1 (B + C + E), B1-2 (B + C + F), B2-1 (B + D + E), B2-2 (B + D + F), each group 8 teeth. All the teeth were prepared and restored accordingly and then mounted on an electronic pressure universal testing machine. The maximum fracture strength and the patterns of failure were recorded., Results: 1) The fracture strength of Group A1-1 > that of Group B1-1, Group A1-2 > Group B1-2, Group B2-1 > Group B1-1, and Group B2-1 > Group B2-2 with significant differences (P < 0.05). 2) The patterns of repairable fracture in Group A1-2 and B1-2 were both 37.5%, and that of the other groups were 0. Furthermore, the difference was significant, and Group A1-2 and B1-2 were higher than other groups., Conclusion: The maintenance of the overhang above the severe wedge-shaped defect aid in the improvement of the fracture strength of the tooth restored with post and core crown. The ferrule of the wedge-shaped defect is not recommended to be prepared. Furthermore, the glass-fiber post and resin core is favorable for the re-repair of the teeth than the cast post and core.

Objective: To explore the relation between plasma neurotransmitters (Glutamic acid, GAA; γ-aminobutyric acid, GABA; 5-hydroxytryptamine, 5-HT; and noradrenaline, NE) and depression in acute hemorrhagic stroke., Methods: Objectives were screened from consecutive hospitalized patients with acute stroke. Fasting blood samples were taken on the day next to hospital admission, and neurotransmitters were examined by the liquid chromatography-high resolution mass spectrometry (LC-HRMS). The fourth edition of Diagnostic and Statistical Manual of Mental Disorders (DSM-IV) was used to diagnose depression at two weeks after onset of stroke. The modified Ranking Scale (mRS) was followed up at 1 year. Pearson test was used to analyse the correlation between serum concentration of neurotransmitters and the Hamilton Depression scale-17-items (HAMD-17) score. Logistic regression was used to analyse the relation of serum concentration of neurotransmitters and depression and outcome of stroke., Results: One hundred and eighty-one patients were included in this study. GABA significantly decreased [6.1(5.0-8.2) µg/L vs 8.1(6.3-14.7) µg/L, P < 0.05] in patients with depression in hemorrhagic stroke, and there was no significant difference in GAA, 5-HT, or NE. GABA concentration was negatively correlated with HAMD-17 score (r = -0.131, P < 0.05); while concentration of serum GABA rose by 1 µg/L, risk of depression in acute phase of hemorrhagic stroke was reduced by 5.6% (OR 0.944, 95%CI 0.893-0.997). While concentration of serum GAA rose by 1 µg/L, risk of worse outcome at 1 year was raised by 0.1%, although a statistic level was on marginal status (OR 1.001, 95%CI 1.000-1.002)., Conclusions: In patients with depression in the acute phase of hemorrhagic stroke, there was a significant reduction in plasma GABA concentration. GABA may have a protective effect on depression in acute phase of hemorrhagic stroke. Increased concentrations of serum GAA may increase the risk of worse outcomes at 1 year after stroke.

Objective: To investigate whether Notch signaling is activated in hepatic stellate cells (HSCs), and to determine whether manipulation of the Notch signaling pathway can effect the activation of HSCs., Methods: The expression of Notch signaling components in unactivated or TGF-b1-activated HSC-T6 cells was detected by Taqman Probe-based gene expression analysis. Differential expression of Notch3 and Jagged1 was detected by immunofluorescence analysis. Notch3-mediated expression of the myofibroblastic markers, a-SMA and collagen I, was detected in HSC-T6 cells transfected with pcDNA3.1-N3ICD or Notch3 siRNA by Western blotting., Results: Notch signaling components were expressed in both unactivated and activated HSC-T6 cells, but the TGF-b1-treated cells showed significantly higher expression levels of Jagged1 (3.9-fold, F = 2543.482), Notch3 (4.2-fold, F = 287.982), and HES1 (3.2-fold, F = 1719.851). Transfection-mediated over-expression of Notch3 led to significantly increased expression of a-SMA (6.8-fold, t = 13.157) and collagen I (5.5-fold, t = 9.810) (both P less than 0.01). Transient knock-down of Notch3 expression by siRNA decreased expression of the myofibroblastic markers (a-SMA by approximately 90%, t = 19.863 and collagen I by 84%, t = 10.376; both, P less than 0.01). Moreover, knock-down of Notch3 antagonized the TGF-b1-induced expression of a-SMA and collagen I., Conclusion: Notch signaling may participate in liver fibrogenesis by regulating HSC activation. Selective interruption of Notch3 may represent a new anti-fibrotic strategy to treat liver fibrosis.

Weak signal, low instrument signal-to-noise ratio, continuous variation of human physiological environment and the interferences from other components in blood make it difficult to extract the blood glucose information from near infrared spectrum in noninvasive blood glucose measurement. The floating-reference method, which analyses the effect of glucose concentration variation on absorption coefficient and scattering coefficient, gets spectrum at the reference point and the measurement point where the light intensity variations from absorption and scattering are counteractive and biggest respectively. By using the spectrum from reference point as reference, floating-reference method can reduce the interferences from variation of physiological environment and experiment circumstance. In the present paper, the effectiveness of floating-reference method working on improving prediction precision and stability was assessed through application experiments. The comparison was made between models whose data were processed with and without floating-reference method. The results showed that the root mean square error of prediction (RMSEP) decreased by 34.7% maximally. The floating-reference method could reduce the influences of changes of samples' state, instrument noises and drift, and improve the models' prediction precision and stability effectively.

Objective: To observe the effect of the combination of Bushen Xuguan Recipe (BXR) and Ruan-jian Huayu Recipe (RHR) on the matrix metalloproteinases-2 (MMP-2) expression of rats with endometriosis (EMS), and to study the pathogenesis and mechanism of EMS., Methods: The EMS rat model was established. Successfully modeled female SD rats were randomly divided into the combination group (BXR+RHR), the RHR group, and the model group. And a normal control group was set up. After 4 weeks of medicinal treatment, rats were sacrificed in the oestrus cycle. MMP-2 distribution and the staining density in ectopic and eutopic endometrium of the rats were observed under microscope. The MMP-2 expression was determined by immunohistochemical assay (SP method)., Results: The MMP-2 expression in the ectopic endometrial tissues of rats in the model group were significantly higher than that of the normal control group (P<0.01). Compared with the model group, the MMP-2 expression decreased in the combination group and the RHR group (P<0.05). The therapeutic effect of the combination group was most obvious by observing the distribution of MMP-2 and changes of positive staining., Conclusions: The occurrence of EMS was correlated with increased activities of MMP-2. The combination method could inhibit the activities of MMP-2 in the ectopic endometrial tissues of EMS rats more effectively than RHR alone, which might be achieved through improving the endocrine environment, and reducing the "invasion" capability of the ectopic endometrium.

Background and Objective: Primary malignant fibrous histiocytoma (MFH) of the lung is a type of rare sarcoma which showed a lack of detailed guidance about treatment and prognosis. The clinical features, treatment methods and prognosis of primary MFH of the lung were analyzed by this study to reveal some information of this disease., Methods: The clinical data and survival state of 20 patients with primary pulmonary MFH treated in Cancer Institute and Hospital of Tianjin Medical University were collected. SPSS 16.0 software was used for statistical analysis. Kaplan-Meier method was applied to figure out whether gender, size, site and post-operative chemotherapy correlated to prognosis. COX regression was employed for multivariate prognostic analysis to find the unattached prognostic factors. Statistical significance was considered at P<0.05., Results: The clinical manifestations of primary pulmonary MFH mainly included cough, blood-stained sputum, chest pain, fever and chest distress. The one-year and two-year overall survival rates were 55.0% and 25.0%, respectively. Most cases died of local recurrence and distant metastasis. Multivariate COX regression analysis showed tumor size and location type were unattached prognostic factors. The patients with tumors smaller than 5 cm had a median survival of 27 months, while larger than 5 cm group's median survival was 8 months. The median survival of central tumor and the peripheral tumor were 6 months and 23 months respectively., Conclusion: Primary pulmonary MFH was a type of highly malignant sarcoma which had poor prognosis. The main effective treatment was surgery, yet the role of postoperative adjuvant chemotherapy in the whole course of treatment was undefined. Small mass size and peripheral type might be correlated to relatively better prognosis.

Objective: To study the mechanism in masticatory muscle dysfunctional induced by hemimastication., Methods: Ca2+ contents were measured with atomic absorption spectrometry; calcinuerin were measured with colorimetric method; muscle fiber types were measured with adenosine-triphosphate (ATPase) staining., Results: (1) Compared with the controls, Ca2+ contents in experimental group had the higher level except 8 weeks (P < 0.05). (2) The ratio of slow fiber in experimental group increased, higher than the match groups (P < 0.05). (3) With Ca2 contents rise, the activities of calcinuerin present a bell-like shape. (4) The ratio of slow-type fiber was positively correlated to the activities of calcinuerin (r = 0.876, P < 0.05)., Conclusion: The signal way of muscle fiber growth and fiber transformation were activated by high concentration of calcium, then, muscle fiber transferred from fast to slow type. It may play an important role in the mechanism that hemimastication result in masticatory muscles dysfunction.

Objective: To study the effect of energy therapy on Ca2+ concentration and Ca2+ -ATP enzyme activity in rat master muscle after unilateral chew, and to discuss the protective action of the exogenous creatine phosphate on rat masseter muscle after unilateral chew., Methods: The 20 rats were randomly divided into 4 groups, A: Creatine phosphate normal control group; B: Creatine phosphate experimental group; C: Saline normal control group; D: Saline experimental group. The Ca2+ concentration were determined by atomic absorption spectrophotometry, the activity of the Ca2+ -ATP enzyme were determined by super-micro volume Ca2+ -ATP enzyme kit., Results: (1) The Ca2+ concentration of the extraction side of group D which received the saline injection had significant difference compared with the non-extraction side (P = 0.007), the group C (P = 0.009) and the extraction side of group B (P = 0.01); (2) Ca2+ -ATP enzyme activity of group D were higher than its non-extraction side (P = 0.001), group C (P = 0.003) and the extraction side of group B (P = 0.001); (3) The ultrastructural changes of the rat masseter muscle under transmission electron microscope were as follows: The extraction side of group D have more severe pathological manifestations than non-extraction side. Both the extraction side and the non-extraction side of group B had a similar manifestation to the normal control group., Conclusion: Exogenous energy material, creatine phosphate, may have certain degree of protective effect on rat masseter muscles after unilateral chew. And it may become a possible way to improve the injury of the masseter muscle.

Group II introns are both catalytic RNAs (ribozymes) and mobile retroelements that were discovered about 14 years ago. Mobile Group II introns were also found in bacteria recently, and they can either retrohome into cognate alleles that lack the intron or retrotranspose to ectopic sites. We reviewed the main mobility (homing) pathways of bacterial group II introns by describing the relationship between Intron-encoded protein activities and intron mobility. We discussed whether mobility of Cyanobacteria group II introns could occur and possible mechanisms of their mobility. Furthermore, the biological implications of group II introns transferring within a species or among different species are also discussed.

Objective: Poor compliance with evidence-based-medicine guidelines could significantly influence the effect of stroke prevention strategies. The objectives of this survey are to investigate the current status in Beijing of cerebral infarction/TIA (transient ischemic attack) inpatients' adherence to the evidence-based-medicine secondary prevention and behavior modifications both at the discharge and at 90-day follow up., Methods: The survey included cerebral infarction/TIA patients successively admitted from 1, Oct 2006 to 1, May 2007, used ACEI/ARB, Statin, anti-platelet agents therapy, accepted carotid endarterctomy or angioplasty or stent, and modified behaviors to stop smoking and control weight. Data of patients during hospitalization were obtained from the medical documents in the inpatient department of 7 centers. The detailed information included the medicine therapy, lifestyle modifications, patients' social demographic background (age, sex, education and way of payment), major risk factors for stroke, and ischemic event subtypes (TIA or cerebral infarction). Above information of part of patients were collected through telephone interview at 90-day follow-up if the patients were not able to visit the clinic., Results: 708 cerebral infarction/TIA inpatients who didn't have contraindications were included. Among them, the patents who haven't used anti-platelet agents, nor taken statin, nor accepted ACEI/ARB were about 0.4%, 41.8% and 63.6% respectively. Neither 27% of the patients have accepted the instructions on stop smoking. While about 588 patients suitable to drug therapy were followed up at 90 days, but the percentage of these patients who haven't compliance on anti-platelet agents, statin, ACEI/ARB was 26.9%, 52.6% and 59.4% respectively. Only 66.9% of the smoking patients have received the doctor instructions on smoking cessation., Conclusion: Stroke recurrence rate in China is higher than that of average figures in western countries. One of the key reasons of high recurrence of stroke in China is the poor adherence to stroke secondary prevention guidelines. Thus, investigate current status of secondary prevention among hospitals in capital of China and promote strategies to enhance the adherence to the guidelines and fill the gap between the clinical practice and evidence-based medicine in China, which is able to lower the stroke recurrence and save lives are urgently needed. Physician should take great care of the patient's compliances on the evidenced-based-medicine secondary prevents and behavior modifications, and take effective measures to improve the compliances.

Background: The role of Preoperative chemotherapy in patients with resected nonsmall-cell lung cancer (NSCLC) remains unclear, this study is aimed at evaluating the effectiveness of Preoperative chemotherapy in patients with resected non-small-cell lung cancer (NSCLC), by performing a meta-analysis of relevant trials., Methods: Searching the literature from MEDLINE, pubmed, OVID, The Cochrane library, Cancerlit, Clinicaltrials.gov, ASCO, ESMO and CBM database, a meta-analysis was carried out to compare patients with NSCLC receiving chemotherapy before surgery with those undergoing surgery alone. The odds ratio (OR) was estimated to assess the survival advantage of Preoperative chemotherapy., Results: Thirteen eligible randomized controlled trials (RCT) were identified, including 2561 patients, the meta-analysis showed preoperative chemotherapy improved survival with a OR of 0.80 (95% confidence interval, 0.68-0.94; P =0.008) in a subset analysis patients with IIIa stage also benefit from the preoperative chemotherapy. There was no evidence of statistical heterogeneity., Conclusions: This analysis shows a significant benefit of preoperative chemotherapy and is currently the best estimate of the effectiveness of this therapy.

Aim: To prepare the fusion protein of a single chain variable fragment 637 (scFv637) against human acetylcholine receptor (AChR) and human serum albumin (HSA) to increase the stability of scFv637., Methods: HSA gene was amplified by PCR and cloned into vector pHEN2 containing a scFv637 gene to construct recombinant vector pHEN2-scFv637-HSA and then transformed into E. coli HB2151 for expression. The expression of fusion protein in periplasm of E. coli was detected by dot hybridization. Relative molecular mass of fusion protein was checked by SDS-PAGE and Western blot., Results: Agarose gel electrophoresis analysis showed that the size of HSA gene and fusion gene was about 1,770 bp and 7,054 bp, respectively. DNA sequencing proved that the nucleotide sequence of constructed scFv637-HSA gene was correct and it was cloned into the open reading frame (ORF) of pHEN2. scFv637-HSA fusion protein only existed in periplasm of E. coli HB2151 transformed with pHEN2-scFv637-HSA. SDS-PAGE and Western blot analysis indicated that relative molecular mass of fusion protein was about 95,900., Conclusion: The scFv637-HSA fusion protein can be successfully expressed in E. coli HB2151, which provides a sound basis for further research into its function and clinical application.

Objective: To study the role of Ca2+ overload and energy metabolism in mitochondria in masticatory muscle dysfunctional induced by occlusal trauma., Methods: Mitochondrial Ca2+ contents were measured with atomic emission spectrophotometer. Mitochondrial ATP and ADP contents were measured with high performance liquid chromatography., Results: (1) Mitochondrial Ca2+ contents of masseter muscle ipsilateral to metal splint in ten and twenty days' experimental groups and that contralateral to metal splint in twenty days' experimental group increased significantly (P < 0.05). (2) Mitochondrial ATP contents of masseter muscle ipsilateral to metal splint in experimental groups were higher than that in control groups and contralateral to metal splint after twenty days. (3) Mitochondrial Ca2+ contents of masseter muscle ipsilateral to metal splint were significantly negatively correlated to the mitochondrial ATP contents (r = -0.780, P < 0.05)., Conclusion: Ca2+ overload in mitochondria depresses ATP production, which results in energy metabolism disorder in masticatory muscle cells. It may play an important role in the mechanism that occlusal trauma results in masticatory muscle dysfunction.

The species diversity and community stability of gap and non-gap stands in Jinyun Mountain were studied by using species richness index(dGL), Shannon-Weiner index, Pielou evenness index(J), Simpson index(D), ecological dominance(lambda), evenness-dominance-abundance index(Z) and community dominance(C). The results showed that the dGL, H', J and D calculated by individuals numbers in gap are 12.14, 4.62, 0.70, 13.32, respectively. Shrub layer plays a greater role than other layers. The corresponding indices in non-gap stand are 6.32, 3.74, 0.66 and 8.16, respectively, which were lower than those in gap. Species diversity indices of the community were far higher in gap than in non-gap. However, ecological dominance and community dominance in gap were smaller than those in non-gap significantly. It suggested that species diversity of the community increased, and community stability decreased, due to the existence of gap formed by natural or human disturbance.

Objective: To investigate the efficacy of IFN-alpha on severe acute pancreatitis (SAP) of IFN-alpha., Methods: A SAP model was developed in adult male rats by retrograde injection of 5% sodium taurocholate in the pancreatic duct. Serum amylase was measured by the blue-starch method and serum cytokines were determined by ELISA., Results: Compared with to the control group, less pancreatic injury and lower amylase level were observed treat in the rats with IFN-alpha. In contrast, the concentration of IL-10 was higher., Conclusion: IFN-alpha has significant curative effect on SAP.

Objective: To investigate the effects of estrogen decreasing on bone tissues around implants in ovariectomized rats., Method: Forty-two female Wistar rats, aged 32 weeks, were used in this study. First, cylindrical hydroxyapatite-coated implants were planted in the proximal part of tibiae of the rats. Then, the rats in experimental group and control group were respectively performed ovariectomy and false surgery after 56 days, the rates were killed in 28, 84 and 168 days after operation, and tibiae were taken out to prepare undecalcified sections. The section were examined by routine histomorphometric measurement and the dynamic changes of trabecular bone and extent of contact between the implants and bone tissue around them., Results: Twenty-eight days after operation, the density of trabecular bone around the implant in experiment group was found significantly reduced compared with the control group. In the area of cortical bone, no difference was found in the extent of implant-bone contact between two groups, while in the area of spongy bone the extent of contact was different obviously., Conclusion: Ovariectomy in Wistar rats can induce significant osteoporosis around implant, these finding suggest that menopause in women patients may also induce osteoporosis around implanted artificial teeth and affect the prognosis. Therefore, the patients should be carefully observed during the implant treatment course.

OBJECTIVE:To study the effect of N(2)O inhalation and propofol intravenous anaesthesia in tooth extraction. METHODS: 40 dental outpatients with the extraction of the mandibular wisdom tooth,ASAI,were randomly allocated to two groups:N(2)O group with the age of 20-36 years old and propofol group with the age of 21-35 years old.All cases undergoing dental extraction were given ultra-light anaesthesia as an adjunct local anaesthesia. RESULTS: The two groups were gained various levels of sedation,there were no significant difference among BP,HR,RR,SPO(2) before,in,and after the operation.Dental treatment could be carried out in cooperating conditin throughout the whole procedure.There was a certain extent of amnesia postoperation,the incidence of postoperative side effects was low,the recovery time was gained within 30 minutes. CONCLUSION: The analgesic effects of N(2)O group were superior to the propofol group.

Objective: To study the advantages of nitrous oxide sedation in teeth extraction., Methods: 112 patients were randomly allocated to four groups: 1) 60 mmol/L nitrous oxide, 2) 60 mmol/L nitrous oxide with estazolam, 3) 40 mmol/L nitrous oxide, 4) 40 mmol/L nitrous oxide with estazolam. The nitrous oxide was used for conscious sedation as an adjunct to local anaesthesia. 53 patients of control group underwent dental extraction with local anaesthesia only., Results: The postoperative MDAS scores of experimental group decreased and was significantly superior to control group (P < 0.01). The patients of experimental group showed a certain levels of sedation and partial amnesia. 97 of the 112 patients (86.62%) preferred inhalation sedation with nitrous oxide. There was no difference between the anxiolytic effects, sedative effects, amnestic effects of the four groups (P > 0.05). Estazolam as preoperative medieation did not show obvious coeffect with nitrous oxide., Conclusion: Inhalation sedation with nitrous oxide can provide effects of goodquality antianxiery, sadation, amnesia in dental extraction.

Objective: To investigate the effects of oxygen free radical on the enhancement of IL-1 activity in vivo and in vitro on hemorrhagic and resuscitated rat., Methods: 30% of rats total blood volume was withdraw by carotid artery catheter and resuscitated with 2 times of lost blood volume 1 h later., Results: Plasm IL-1 activity and MDA content increased and SOD activity decreased significantly 1-4 hours after resuscitation. There was a marked correlation between IL-1 activity and MDA content as well as SOD activity. Treatment with SOD as resuscitation prevented the postresuscitation increase in plasma IL-1 activity significantly. Hemorrhage and resuscitation also caused significant decrease of SOD activity and elevation of MDA in peritoneal macrophage 2 hours after resuscitation. After preincubation with SOD for 1 hour, the macrophage presented a much lowered capacity to release IL-1., Conclusion: Oxygen free radical may be one of the most important factors that contribute to elevation of IL-1 level after hemorrhage and resucitation.

DNA content was quantitatively determined in 30 cases of salivary gland carcinoma (SGC) by image analysis technique using paraffin-embedded sections stained with Feulgen method. The results showed that the nuclear DNA content was related to the malignancy of SGC, based on the clinical and morphological criteria such as tumor size, mobility, property of growth, mitotic rate, degree of differentiation and the histological type of the tumor. The number of nuclei above 5 C DNA, expressing aneuploidy, seems to be of more prognostic significance. Aneuploidy greater than 10% may be indicative of a poor prognosis for SGC. Thus, it is suggested that the quantitative analysis of DNA content may be able to provide an objective and quantitative reference for evaluating the prognosis of SGC.