1. [Triterpenoids from Inonotus obliquus protect mice against oxidative damage induced by CCl4].
- Author
-
Zhao FQ, Yan L, Cui XH, Lin S, Wang C, Zhang H, Kang XY, and Ji BS
- Subjects
- Alanine Transaminase blood, Animals, Aspartate Aminotransferases blood, Carbon Tetrachloride, Catalase blood, Catalase metabolism, Female, Glutathione blood, Glutathione metabolism, Glutathione Peroxidase blood, Glutathione Peroxidase metabolism, Interleukin-6 blood, Lanosterol analogs & derivatives, Lanosterol isolation & purification, Lanosterol pharmacology, Liver metabolism, Male, Malondialdehyde blood, Malondialdehyde metabolism, Mice, Protective Agents isolation & purification, Random Allocation, Superoxide Dismutase blood, Superoxide Dismutase metabolism, Triterpenes isolation & purification, Oxidative Stress drug effects, Polyporaceae chemistry, Protective Agents pharmacology, Triterpenes pharmacology
- Abstract
To investigate the effects of lanosterol (1), inotodiol (2) and trametenolic acid (3) from Inonotus obliquus against oxidative damage induced by CCl4 in mice, 1, 2 and 3 (20, 10 and 5 mg x kg(-1)) were respectively administered to mice, once a day for 3 days. Then the mice were induced to oxidative damage by CCl4 on the third day 30 min after the administration. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-PX) and the content of malondialdehyde (MDA) and reductive glutathione (GSH) in serum and liver homogenate were determined. And the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and interleukin-6 (IL-6) concentration in serum were detected. The results showed that treatment with compound 1, 2 and 3 could significantly increase the activities of SOD, CAT and GSH-PX in serum and liver homogenate. Furthermore, the content of GSH in serum and liver homogenate increased and MDA content decreased markedly. In addition, compound 1, 2 and 3 could significantly inhibit the activities of ALT and AST in serum, and decrease the IL-6 concentration in serum remarkably. So, compound 1, 2 and 3 can protect mice against oxidative stress injury induced by CCl4. Furthermore, compound 1, 2 and 3 can protect cells from damage through inhibition on ALT, AST and the expression of IL-6.
- Published
- 2012