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In view of the problem of circulating cooling system blow-down water treatment in a power plant，a scheme to reduce the salt content and sulfate concentration in the circulating cooling system blow-down water was proposed by using nano-filtration technology to meet the local environmental protection standards and realize the up-to-standard discharge. Moreover， a technical and economic comparison with the circulating water bypass desalination treatment scheme using reverse osmosis technology were made. The impact of the two schemes on the zero discharge of desulfurization waste water from the power plant were fully considered in the comparison and selection of the schemes. The result showed that the scheme of standard discharge after nano-filtration treatment had more advantages.

Aiming at various genetic algorithms commonly used in composite layer optimization, it is difficult to comprehensively consider complex engineering constraints such as surface 45°, layering ratio, and continuous layer number limit, in this paper, an isogenic sequence double random genetic algorithm is proposed for composite laminates. The introduction of double random algorithms in the exchange and mutation operators ensures the strict equality of gene sequences in the optimization iteration, and based on the SABRE software the optimization method is realized, and the correctness of the algorithm is verified through numerical examples. The results show that the proposed algorithm can fit complex engineering constraints such as balance, symmetry, surface 45°, continuous ply number limit, ply ratio, etc., and has high reliability and engineering applicability.

Objective To observe the clinical characteristics of Parkinson's disease (PD) patients complicated with Pisa syndrome (PS), and to explore the influencing factors for PS occurrence in the PD patients. Methods A nested case-control study was conducted on 222 patients continuously diagnosed with PD but not with lateral spinal flexion admitted in our department from September 2020 to December 2020. After 2 years of follow-up, 26 PD patients complicated with PS were assigned into observation group, and 98 sex- and age-matched PD patients without PS complication were assigned into control group in a ratio of 1 ∶4. Their demographic and clinical data were collected, and the relevant scales and questionnaires were performed, including Hoehn and Yahr (H-Y) Staging Scale, Parkinson's Disease Questionnaire-8 (PDQ-8), Unified Parkinson's Disease Rating Scale (UPDRS), Visual Analogue Scale (VAS), Montreal Cognitive Assessment Scale (MoCA), Hamilton Anxiety Scale (HAMA) and Hamilton Depression Scale (HAMD). Results There were significant differences between the observation group and the control group in the course of the disease, equivalent daily dose of levodopa, drug adjustment, repeated falls within half a year, H-Y stage, UPDRS-Ⅲ score, score of axial symptoms, rigidity score, VAS score, PDQ-8 score, HAMA score, HAMD score and MoCA score (P < 0.05). Multivariate logistic regression analysis showed that the occurrence of PS was closely correlated with score of axial symptoms (OR=1.402, 95%CI=1.050~1.872, P=0.022), rigidity score (OR=1.429, 95%CI: 1.053~1.937, P=0.022) and VAS score (OR=1.772, 95%CI: 1.089~2.883, P=0.021). ROC curve analysis indicated that the AUC value of score of axial symptoms, rigidity score and VAS score was 0.899, 0.835 and 0.827, with a Youden index of 0.681, 0.558 and 0.488, respectively. Conclusion The severities of axial symptoms, rigidity and low back pain in PD patients are closely associated with the occurrence of PS.

Objective To explore the efficacy of wrapping the hepatic artery with detached ligamentum teres hepatis during pancreaticoduodenectomy (PD) on preventing postoperative intra-abdominal hemorrhage. Methods A total of 186 patients who received PD in the First Affiliated Hospital of Xinjiang Medical University from June 2020 to June 2022 were divided into wrapping group (n=86) and non-wrapping group (n=100) according to whether the detached ligamentum teres hepatis was used to wrap the hepatic artery during the operation. The clinical data on perioperative and postoperative complications were collected and retrospectively analyzed in two groups. Results PD was successfully performed and completed in both groups, and there was no significant difference in age, gender, BMI, length of hospital stay, operative time and intra-operative blood loss between two groups (P＞0.05) . The incidences of pancreatic fistula, biliary fistula and abdominal infection were similar in two groups (P＞0.05) , but the incidence of postoperative bleeding in wrapping group was significantly lower than that in non-wrapping group (1.16% vs 9.00%, P＜0.05) . Logistic regression analysis showed that the pancreatic and biliary fistulas of grade B/C were the independent risk factors for postoperative abdominal bleeding after PD (P＜0.05) , and the wrapping of the hepatic artery with the detached hepatic round ligament during operation was an independent protective factor for postoperative abdominal bleeding after PD (P＜0.05) . Conclusion Wrapping the hepatic artery with detached ligamentum teres hepatis during PD surgery may reduce the incidence of postoperative abdominal bleeding and is simple, safe and feasible to perform without additional complications．

Objective: This study aimed to improve the flavor of Cantonese sausage and innovate the product. Methods: The volatile flavor compounds in the Cantonese sausages containing sauce-flavor Baijiu air-dried for 0 day, 2 days and 4 days were detected by GC-MS, and the changes of volatile flavor compounds were analyzed by multivariate statistics. Results: A total of 39 volatile flavor compounds were detected through GC-MS technology, including 18 hydrocarbons, 12 esters, 2 aldehydes, 2 ethers, 1 alcohol, 1 acid, 1 ketone and 2 other compounds. Esters, aldehydes, alcohols and hydrocarbons significantly increased during air-drying, while ketones and ethers showed a trend of first increasing and then decreasing during air-drying. The acids did not change significantly during air-drying. Five different flavor substances were identified through VIP values, which could be served as potential biomarkers for distinguishing sausages with different air-drying times. Six key flavor components were screened through OAV. Ethyl 2-methylbutyrate, ethyl hexanoate, and ethyl butyrate were common key flavor components in three groups of sausages, and their content significantly increased during air-drying. Conclusion: Adding sauce-flavor Baijiu can increase the types and contents of volatile flavor compounds in Cantonese sausage and promote the formation of new flavor compounds.

In order to solve the problem of low connection strength between elastic skin and honeycomb sandwich of composite flexible skin for variant aircraft, a “two-step bonding” connection scheme is proposed. Firstly, the polyurethane fiber cloth and honeycomb are bonded by epoxy resin structural adhesive, then the silicone rubber sheet and the spandex fiber cloth are bonded by the silicone rubber adhesive, and the manufacturing method is given. Finally, “two-step bonding” experimental piece and “direct bonding” experimental piece were prepared respectively, and the bonding strength experiment and comparative analysis were carried out, The results show that the bonding strength of the “two-step bonding” experimental piece is five times that of the “direct bonding” experimental piece.

To enhance the health detection capability of the semi-flexible plate nozzle in a supersonic wind tunnel, a large-deflection deformation reconstruction algorithm for a semi-flexible plate nozzle based on the strain-moment relationship is developed. The multi-jack semi-flexible plate nozzle in the supersonic wind tunnel is studied; its mechanical model of contour assembly is established. The differential equation of flexible plate contour curve is deduced, and the elliptic integral expression of the flexible plate deformation is given. Based on the deformation theory of a rectangular thin plate, the deformation reconstruction algorithm is modified, and its correction effect is simulated and analysed with the finite element method. The maximum deviation after correction is only 10.78% compared with that before correction. The 0.3 m semi-flexible plate nozzle is used as the experimental platform to carry out the experimental study. The experimental results show that under the 6 Mach number, the large-deflection deformation reconstruction results based on strain-moment relationship are in good agreement with the experimental results. The coordinate deviation of the nozzle throat position is about 6.12% compared with the structural deformation, thus verifying that the deformation reconstruction algorithm in this paper is effective and accurate.

Natural language video localization(NLVL),which aims to locate a target moment from a video that semantically corresponds to a text query,is a novel and challenging task.Different from the task of temporal action localization,NLVL is more flexible without restrictions from predefined action categories.Meanwhile,NLVL is more challenging since it requires align semantic information from both visual and textual modalities.Besides,how to obtain the final timestamp from the alignment relationship is also a tough task.This paper first proposes the pipeline of NLVL,and then categorizes them into supervised and weakly-supervised methods according to whether there is supervised information,following by the analysis of the strengths and weaknesses of each kind of method.Subsequently,the dataset,evaluation protocols and the general performance analysis are presented.Finally,the possible perspectives are obtained by summarizing the existing methods.

ObjectiveTo explore the application value of critical view dissection guided by Rouvieres sulcus in laparoscopic cholecystectomy （LC）. MethodsRetrospective analysis was performed on 232 patients received LC in Yueyang Hospital of Integrated Traditional Chinese and Western Medicine from August 2018 to July 2020. Patients underwent critical view dissection guided by Rouvieres sulcus were served as study group（n=141）, and patients underwent traditional anatomical dissection as control group （n=91）. The operation time, intraoperative bleeding, the number of drains, bile duct injury and laparoscopy converted to laparotomy, the time of first postoperative feeding and exhaust, the number of postoperative infections, the time of discharge were compared between two groups. ResultsCompared with the control group, the study group had shorter operation time ［（50.07±5.91）min vs（60.48±13.30）min, t=8.14, P＜0.05］, lower intraoperative bleeding ［（13.42±7.93）mL vs （25.02±21.17）mL, t=5.90, P＜0.05］, lower placement drainage rate, bile duct injury rate, postoperative infection rate, and earlier postoperative feeding, exhaust, and discharge time, with statistically significant differences （P＜0.05）. ConclusionCompared with traditional anatomical dissection method, critical view dissection guided by Rouvieres sulcus in LC can shorten surgical time, reduce intraoperative blood loss, reduce the probability of bile duct injury, and accelerate postoperative recovery, which is worthy of clinical application and promotion.

The multi-channel parallel diode structure is effective in obtaining X-ray with low energy, large area coverage, and high dose rate in laboratory conditions. In this study, the dose uniformity of single- and five-ring structures was studied based on a point source model. The results showed that the radiation field was primarily focused on the projection position of the ring in the plane near the ring; as the distance increased, the radiation was gradually dispersed to other regions. In the case of single ring structure diodes and z < 15 cm, 80% of the area of the dose homogeneously increased and then decreased with the increasing distance, whereas the total dose in the calculated region decreased gradually. Considering the dose uniformity and total dose, the optimal experimental region of single ring diode radiation field was z = 7-9 cm. A smaller inner ring radius will only enhance the dose near the projection point of the ring; however, the uniformity of the dose on the plane will decrease. A larger inner ring radius will also decreased the dose near the projection point of the ring center. When the outer radius of ring was 10 cm, the optimal inner radius was 8-9.5 cm. Compared with the single ring diode, the dose uniformity of the five ring diode can be greatly enhanced, and the dose uniformity of radiation field and effective experimental region can be improved. Moreover, in z = 11-17 cm, the five-ring dose uniformity did not exhibit significant fluctuations.

The methods of consistency evaluation are introduced for the finite element simulation and test of the aircraft structure. First,the strain interpolation method is studied based on the element shape functions,and the transformation method is given for the consistency between simulation and test strain gauges; Second,linearization correction for the test strain gauges was obtained by using the least squares principle. The consistency evaluation methods are investigated based on graph partition and probability respectively. Finally consistency evaluation is carried out for the computation and test of the civil aircraft structure. It is shown that the evaluation results can provide support for the modification of the model.

BACKGROUND The chemical composition of biotite contains important genetic information. The in-situ analyses of biotite with metasomatic texture can reflect the migration of the elements during the metasomatic alteration, and provide mineralogical evidence for the ore-forming mechanism. OBJECTIVES To investigate the migration of elements during trachyte alteration and reveal the Nb mineralization mechanism in altered trachyte. METHODS Biotite phenocryst with reequilibration texture in altered trachyte in the Pingli area of North Dabashan was studied, and the EMPA element mapping and line profile analyses were carried out. RESULTS The results show that the phenocryst was magmatic in the core and the reequilibrated origin on the rim, caused by diffusion-reaction process. Compared with the core, the TiO2 content decreased from an average of 5.78% to 2.22% in the rim, indicating that Ti was leached out into the fluid during the exchange alteration. The CaO content increased from 0.06% to 0.14% on average, and the F content increased from 0.60% to 0.80% on average, suggesting that the alteration fluid is rich in Ca and F. CONCLUSIONS The formation of Nb deposits in trachyte is closely related to the hydrothermal activity, and the components such as Ca and F in the ore-forming fluid play an important role in the migration and enrichment of Nb.

Breast tumor is a kind of woman′s disease with high incidence rate, it is also a kind of disease that can be diagnosed early and treated early. Thus the mortality rate could be reduced. The method of combining grey symbiotic matrix with BP neural network is proposed to improve the recognition rate of breast tumors. Firstly, the infrared breast image was pretreated to highlight the texture of lesions and blood vessels, and the texture features of such gray-scale curve images of the breast were extracted by using the gray co-occurrence matrix. Then, the sample data were trained through BP neural network. The trained BP neural network could effectively identify the lesion area. The experimental results show that the method presented in this paper has good recognition effect on the breast tumor lesion area.

According to the different concentration levels of Doppler spectrum between sea clutter and target, small target in sea clutter background can be detected using Shannon entropy. However, Shannon entropy is merely a special case of Tsallis entropy and cannot reflect the multifractality of sea clutter. In this paper, the relation between Tsallis entropy and the generalized fractal dimension is first presented, and then the Doppler spectrum’s concentrative level and multifractality of sea clutter are combined; finally an algorithm for detecting small target in sea clutter background based on Tsallis entropy of Doppler spectrum rather than of Shannon entropy is proposed. By comparison via IPIX dataset, the detection’s performance of Tsallis entropy is better than that of Shannon entropy and Hurst exponent as per short observations.

Objective To analyze the initial displacement of the upper central incisor and stress distribution of periodontal ligament under different torque values of upper incisors under the action of a four⁃curved auxiliary arch to provide a reliable basis for the safety of clinical application of four⁃curved auxiliary arches. Methods A three⁃dimen⁃ sional finite element model for torque control of upper anterior teeth with a homemade quadrilateral auxiliary arch was established. Four different states were analyzed: molar ligation without extraction space (group A), microimplant ligation without extraction space (group B), molar recovery with extraction space closure (group C) (the adductive traction force was set at 115 g) and microimplant recovery with extraction space closure (group D) (the adductive traction force was set at 115 g). When four types of torque (0.5 N, 1.0 N, 1.5 N, and 2.0 N) were applied. The initial displacement of upper central incisors and the stress distribution of periodontal ligament in 16 groups (A1⁃A4, B1⁃B4, C1⁃C4, D1⁃D4) were ob⁃ served. Results Under different conditions, as the strength of the four⁃curve auxiliary arch increases, the maxillary an⁃ terior teeth has crown labial inclination and a root lingual inclination. The displacement of the incisor tip increases with the increase in the loading force of the torque auxiliary arch, and the displacement of the incisor root apex increases as the force increases. The difference in incisor⁃apex displacement distance in A1⁃A4, B1⁃B4, C1⁃C4, D2 and D4 groups increased as the torque force increases, while the difference between the D3 group and D1 and D2 groups decreased slightly. The stress of the cervical periodontal ligament of the upper central incisor did not exceed the stress of the peri⁃ odontal ligament in the following groups: A1, A2, B1, B2, B3, C1, C2, D1, and D2. The stress of the lip side of the up⁃ per central incisor did exceed the stress of the periodontal ligament in the following groups: A3, A4, B4, C3, C4, D3, and D4. In other words, when using the four⁃curved auxiliary arch as an implant anchorage, the force applied in the ab⁃ sence of extraction space should not exceed 1.5 N, and the force applied in the adduction of extraction space should not exceed 1.0 N. When using the nonimplant anchorage, the force applied in the absence of extraction space and the ad⁃ duction of extraction space should not exceed 1.0 N. In addition, the range of force should not exceed the maximum stress of the periodontal ligament in the cervical region such that the effective and safe torque movement can be achieved. Under other stress conditions, the stress of the labial and cervical periodontal ligament of the upper central in⁃ cisor exceeded the stress value (2.6 × 10 ⁃ 2 MPa). The stress value of periodontal ligament was 2.6 × 10 ⁃ 2 MPa in all groups. Conclusion A four⁃curved auxiliary arch has a significant effect on the upper anterior teeth, and the use of mi⁃ croimplants can better control root movement such that the crown of upper central incisors cannot be excessively lip in⁃ clined.

It is well known that the fuselage and wing mainly consist of thin-skin stiffened panels and the failure load of stiffened panels is hot topic in aircraft engineering. Johnson-Euler equation is used to evaluate the failure loads, thus the effective width computation of skin needs to be determined. Several kinds of methods to calculate the effective width of skin are analyzed to quest for its origin, and their applicability is verified with test results of thin-skin stiffened panel. The results show that, the calculation errors of the effective width calculation methods are less than 10% for the calculation of riveting Z-typed fuselage stiffened panel failure load, and the methods can be helpful to engineers who design thin-skin stiffened panels in compression.

According to the existing Retinex algorithm can not automatically adjust the parameters, an adaptive bilateral filter Retinex algorithm based on parameter estimation was proposed. Firstly, the principal component analysis and the Canny edge detection algorithm are used to estimate the noise and edge intensity. Then the spatial geometric standard deviation parameter and the luminance standard deviation parameter of the bilateral filter are calculated by linear correlation calculation. And the bilateral filter of the parameter estimation is used to decompose the image and the reflected image. Finally, the illumination image and the reflected image are compressed and enhanced in different ways and a new image is synthesized. The experimental results show that the algorithm can not only automatically set the parameters, but also effectively inhibit the halo phenomenon.

The FEM has been widely adopted to solve electromagnetic field problems. The accuracy of FEM is highly effected by the number of system units and the complexity of computational order,leading to low computational efficiency. In order to improve the computational efficiency of FEM,gradient smooth is introduced into numerical calculation of electromagnetic field,then a new method of ES-FEM is proposed. The electromagnetic field of infinite straight grounding slot and Electric Vehicle are simulated with MATLAB. The results shows that smooth gradient operation makes the discrete system closer to the continuous system,increasing the accuracy and the efficiency of FEM.

The advantages and disadvantages of existing extraction method about planar linkage curve characteristic parameter are analyzed and summarized.On the basis of the property that high-frequency component of the Fourier descriptor can well explain the details,and low-frequency component determines the overall shape of the object boundary,the method by using Fourier descriptor to extract characteristic parameters of the planar linkage curve is proposed,and the numerical atlas database of planar linkage curve characteristic parameter is established.The calculation results show that the low-frequency component of Fourier descriptor can well reproduce the original closed curve through the inverse Fourier transform,and the normalized Fourier descriptor has good robustness,the corresponding numerical atlas database has the advantages of high precision,simple calculation,small redundancy.All of these bring a new thinking way for four-bar linkage curve replay.

A novel planar two degree of freedom parallel manipulator is presented,its moving platform of the manipulator has smaller inertia and the output of the manipulator is planar translational motion.Unlike the traditional optimum analysis method,not only the geometric parameters,but also the range of the linear actuators of the manipulator are considered as the kinematics parameters,the design space is obtained by normalizing the actuation and geometric parameter.The dexterity performance index is calculated in the design space,the dexterity atlas of the manipulator is plotted.The dexterity atlas reflects the relationship between the dexterity performance index and the kinematics parameters of the manipulator.The dexterity atlas is very useful for the design of the new manipulator and the new method also provides a simple,comprehensive and effective way for design parallel manipulator that is actuated by linear actuators.

The paper introduced concepts of cloud computing and Internet of Things, analyzed inevitability and combination mode of the cloud computing and the Internet of Things. It proposed architecture of the Internet of Things based on cloud computing and studied security threats of the Internet of Things based on the cloud computing and gave corresponding security solution including how to construct trusted environment for the Internet of Things and how to protect data security in the cloud of the Internet of Things. It pointed out technical difficulties of the security research and research status of the Internet of Things based on the cloud computing.

Background and objective Excision repair cross-complementing 1 (Excision-Repair Cross-Complementing 1, ERCC1), an important member of the DNA repair gene family, plays a key role in nucleotide excision repair and apoptosis of tumor cells. Protein kinase C-α (Protein kinase C, PKCα), an isozyme in protein kinase C family, is an important signaling molecule in signal transduction pathways of tumors, which has been implicated in malignant transformation and proliferation. The aim of this study was to explore the clinical significance of ERCC1 and PKCα in non-small cell lung cancer (NSCLC). Methods The expression of ERCC1 and PKCα were examined by immunohistochemistry (IHC) in the specimens of 51 cases of NSCLC patients tissue and 21 cases of paracancerous tissue. The relationship between detected data and patients′ clinical parameters was analyzed by SPSS 13.0 software. Results The positive expression rate of ERCC1 and PKCα in NSCLC tissues was significantly higher than paracancerous tissues (Ρ

A modified security model based on dynamic trusted degree security model MSMBDTD（modified security model based on dynamic trusted degree） was proposed.Based on the trusted authentication and measurement of trusted computing,MSMBDTD first gave definition of trusted degree rule.Then it analyzed dynamic transformation of trusted degree in the course of system running.And also it modified access privilege of subject,which made access range be in conformance with trusted degree.Finally,after formal description and safety analysis with proof are given,MSMBDTD can support access control based on trusted degree.

Objective: To explore the role of tumor necrosis factor receptor-associated factor 4 (TRAF4) in promoting the abnormal activation of epidermal growth factor receptor (EGFR) and its effect on lung cancer cell proliferation, migration and invasion. Methods: Tumor tissues from patients who underwent lung adenocarcinoma resection at The First Affiliated Hospital of Second Military Medical University, from January 2015 to May 2017 were collected, and the expressions of TRAF4 and Ki-67 in lung cancer tissues were detected by immunohistochemistry, the mRNA levels of Cyclin D and Vimentin were detected by real-time fluorescence quantitative PCR (qRT-PCR). The effect of TRAF4 on the tumor growth ability of lung cancer A549 cells was investigated by the xenograft model, the effect of TRAF4 or EGFR on the tumor proliferation ability was detected by using cell counting kit 8 (CCK8) and BrdU assay, and the migration and invasion abilities of tumor cells were detected by Transwell assay. Different structural domain deletion expression vectors of TRAF4 and EGFR were constructed to transfect cells, and the interaction mode of TRAF4 and EGFR was investigated by immunoprecipitation assay. Results: The expression of TRAF4 in non-small cell lung cancer (NSCLC) tissues was positively correlated with the expressions of Ki-67, cyclin D, and vimentin ( r 2 : 0.438, 0.695, and 0.736, respectively, all P ＜0.01). Immunohistochemical assay of tumor tissues from NSCLC patients showed that tissues with high expression of TRAF4 were also high in Ki-67. Patients with high TRAF4 expression (TRAF4 positivity >30%) had a shorter progression-free survival (PFS) time than that of patients with low TRAF4 expression (TRAF4 positivity ≤30%) (median PFS of 12 and 19 months, respectively; P =0.034). Traf4 -/- cells had a weakened proliferative capacity than traf4 +/+ cells and formed tumors with smaller size ( P ＜0.05). The expression level of Ki-67 in the tumor tissues formed by traf4 -/- cells [(45.6±8.7)%] was lower than that in the tumor tissues formed by traf4 +/+ cells [(62.3±10.3)%, P =0.015], the mRNA levels of cyclin D (1.01±0.15) and vimentin (1.01±0.12) in the traf4 -/- cells were lower than those of the traf4 +/+ cells (3.41±0.32 and 3.12±0.18, respectively, both P ＜0.05).The western blot results showed that, with the elevated intracellular expression level of TRAF4, phosphorylation level of EGFR was significantly increased in both wild-type EGFR and activation mutant EGFR-expression cells. The capacities of proliferation, migration and invasion of A549 cells was weakened after EGFR knockdown (all P ＜0.01). Immunoprecipitation experiments showed that TRAF4 binds to the peptide segment of the near-membrane region of EGFR through the TRAF structural domain, and the mutual binding between EGFR molecules was enhanced under TRAF4 overexpression conditions. Increasing TRAF4 expression promoted EGFR molecular phosphorylation and activation of downstream signaling. Conclusions: TRAF4 expression is elevated in NSCLC tissues and tumor cells, which promotes tumor proliferation, migration and invasion. TRAF4 directly binds to EGFR molecules, enhances its own phosphorylation and activates the downstream signaling pathway by promoting the interaction between EGFR molecules.

Objective: To explore the safety and effectiveness of early stent implantation in patients with acute anterior circulation large artery disease. Methods: Patients were recruited from the RESCUE-RE study (a registration study for Critical Care of Acute Ischemic Stroke After Recanalization). Patients who were diagnosed with acute ischemic stroke within 24 hours of onset and given endovascular treatment after consultation from July 2018 to May 2019 from 18 sub-centers nationwide were retrospectively enrolled. According to whether the stents were placed during the operation, the patients were divided into two groups: stenting group and non-stenting group. The baseline between the two groups was matched by propensity score. The matching variables included age, sex, baseline National Institutes of Health Stroke Scale (NIHSS) score, baseline Glasgow Coma Scale (GCS) score, history of stroke, smoking and onset to hospital time. The primary clinical outcome was 90-day good neurological outcome [defined as modified Rankin score (mRS) 0-2]. Secondary outcomes include 90-day mortality, 24-hour re-occlusion of the responsible artery, and symptomatic intracranial hemorrhage. The differences in clinical endpoints between the two groups were compared. Result: A total of 899 patients with acute anterior circulation artery stenosis or occlusion were included in the study, with a mean age of(66±12)years,and 532(59.18%) were male. There were 193 patients in the stenting group and 706 patients in the non-stenting group. After the baseline data between the two groups were matched by propensity score, 169 patients were enrolled in each of two groups respectively. After matching, the proportion of patients in the stenting group with etiological diagnosis of large atherosclerosis [82.53% (137/166) vs 55.69% (93/167)] and the proportion of patients with previous history of hypertension [63.31% (107/169) vs 47.93% (81/169)] in the stenting group were higher than those in the non-stenting group (both P <0.05). While the proportion of patients in the non-stenting group with cardiogenic embolism [37.73%(63/167) vs 11.45%(19/166)]and the proportion of patients with atrial fibrillation [18.93% (32/169) vs 10.65%(18/169)]was higher(all P <0.05). In the stenting group, the time from onset to recanalization was longer[519 (408, 620)min vs 469 (365, 690)min], and the proportion of general anesthesia [50.89% (86/169) vs 35.50% (60/169)] was higher in the stenting group(both P <0.05). In addition, in the stenting group, the proportion of patients receiving mechanical thrombectomy[67.46% (114/169) vs 88.76% (150/169)] and arterial thrombolysis [2.37% (4/169) vs 18.93% (32/169)] was lower than non-stenting group during the operation, while the proportion of patients receiving balloon dilation [53.85% (91/169) vs 13.61% (23/169)]was higher(both P <0.05). The proportion of patients in stent group receiving antiplatelet drugs before operation was higher [13.46% (21/169) vs 8.70% (14/169)](both P <0.05). In terms of clinical outcome, compared with the non-stenting group, the proportion of patients in the stenting group with good neurological function in 90 days was lower [44.79% (73/169) vs 56.36% (93/169)], and the proportion of death at 90 days was higher[15.98% (27/169) vs 8.88% (15/169)] (both P <0.05). There was no significant difference between the two groups in 24-hour re-occlusion[8.88% (15/169) vs 9.47% (16/169)] and symptomatic intracranial hemorrhage[5.92% (10/169) vs 4.76% (8/169)](both P >0.05). Conclusion: For patients with acute anterior circulation artery disease, early stent therapy may increase the proportion of patients with adverse neurological outcomes.

Children with low immunity and weak resistance are at high risk for influenza, and infection with the influenza virus can be life-threatening in severe cases. This article reported on the investigation and management of a clustered of influenza A cases in a pediatric ward. The clustered influenza A cases were caused by a community-infected influenza A chaperone who was the source of infection, and was most likely spread in close proximity through droplets or aerosols in the same hospital ward. This person caused the influenza A hospital-acquired infections in 2 children (aged 11 months and 12 months, respectively) in the hospital. After taking timely case management and ward management, the prognosis of the three infected patients was good and there was no outbreak of influenza-like illness in the pediatric ward. It is recommended that during the peak of the influenza season, air disinfection should be strengthened and windows should be opened for ventilation in medical institutions, while patients and accompanying staff should be strengthened to wear masks and other publicity, and influenza vaccination should be encouraged, so as to reduce cross-transmission and prevent outbreaks of hospital-acquired infections.

Objective: To explore the expression and the role of chemerin in idiopathic pulmonary fibrosis (IPF). Methods: Quantitative PCR and Western blotting were used to determine the mRNA and protein levels of chemerin in lung tissues from IPF patients and the controls. Clinical serum level of chemerin was analyzed by enzyme-linked immunosorbent assay. The mouse lung fibroblasts isolated and cultured in vitro were divided into the control, TGF-β, TGF-β+chemerin and chemerin groups. Immunofluorescence staining was used to observe the expression of α-smooth muscle actin (α-SMA). C57BL/6 mice were randomly divided into the control, bleomycin, bleomycin+chemerin, and chemerin groups. Masson and immunohistochemical staining were performed to evaluate the severity of pulmonary fibrosis. Expression of epithelial to mesenchymal transition (EMT) markers was detected by quantitative PCR and immunohistochemical staining in the in vitro and in vivo models of pulmonary fibrosis, respectively. Results: Compared with the control group, the expression of chemerin was downregulated in both the lung tissue and the serum of IPF patients. Immunofluorescence showed that treatment of fibroblasts with TGF-β alone resulted in a robust expression of α-SMA, whereas treatment with TGF-β and chemerin together exhibited the similar expression levels of α-SMA as the control group. Masson staining indicated that the bleomycin-induced pulmonary fibrosis model was constructed successfully, while treatment of chemerin partially alleviated the damage of lung tissue. Immunohistochemical staining showed that the expression of chemerin in the lung tissue was significantly decreased in the bleomycin group. Quantitative PCR and immunohistochemistry showed that chemerin attenuated EMT induced by TGF-β and bleomycin both in vitro and in vivo . Conclusions: The expression of chemerin was reduced in patients with IPF. Chemerin may play a protective role in the development of IPF by regulating EMT, providing a new idea for the clinical treatment of IPF.

Objective: To study the expression of tyrosine kinase receptor 2 (Tie2) in oral squamous cell carcinoma (OSCC) and its effect on cell proliferation and migration and the epithelial-mesenchymal transition (EMT) process., Methods: Immunohistochemistry (IHC) tests were conducted to examine the expression of Tie2 in OSCC tissues and normal oral mucosa tissues. Western blot was performed to examine the expression of Tie2 in dysplastic oral keratinocyte (DOK) cell line and OSCC cell lines, and the cell line with high Tie2 expression was selected as the experimental cell line. The Tie 2-silenced lentiviral vector was successfully transfected onto the experimental cell line for subsequent experiments. Cell proliferation and cloning abilities were examined with CCK-8 and clone formation assays. Cell migration ability was examined with scratch and Transwell assays. The remodeling ability of cytoskeletal F-actin and the expressions of E-cadherin and N-cadherin were examined with confocal laser scanning microscope. Western blot was performed to examine the expression of EMT-related signature proteins, including E-cadherin, N-cadherin, and vimentin, and the expression of the protein kinase B (AKT) and extracellular signal-regulated kinase (ERK)., Results: IHC results showed that the Tie2-positive rate of the OSCC group (74.5%) was significantly higher than that of the control group (19.4%) ( P <0.0001). The expression of Tie2 was higher in HSC-4 and SCC-9 cell lines compared to that in DOK cells. The lentiviral shRNA-162 group showed the best silencing effect, which was used as the experimental group and applied in subsequent experiments. Compared with those of the control group, the proliferation, cloning and migration capacities of the cells of the experimental group were significantly reduced. Furthermore, the green fluorescence intensity of the cytoskeleton F-actin was reduced, the number of filamentous pseudopods at the leading edge of the cells decreased and their length was shortened, and the expression of E-cadherin was significantly increased, while the expression of N-cadherin and vimentin was significantly reduced in the experimental group in comparison with those of the control group. The expression of p-AKT and p-ERK proteins decreased, while AKT and ERK protein expression increased., Conclusion: Tie2 was highly expressed in most OSCC cells. Silencing Tie2 can inhibit the proliferation, cloning, and migration ability of OSCC cells, inhibit F-actin remodeling, and alter the expression of its EMT-related signature proteins by regulating AKT and ERK signaling pathway, which suggests that Tie2 may be involved in the growth, metastasis and EMT process of OSCC., (Copyright© by Editorial Board of Journal of Sichuan University (Medical Sciences).)

Objective: To analyze the efficacy of using baseline calcitonin (bCtn) for auxiliary diagnosis of medullary thyroid cancer (MTC) in the hypercalcitoninemic population with thyroid nodules and to explore the relationship between preoperative levels of bCtn and carcinoembryonic antigen (CEA) and MTC staging., Methods: The clinical, pathological, imaging, and lab test data of 58 MTC patients and 84 non-MTC patients were retrospectively reviewed in the study. The patients were hospitalized at West China Hosptal, Sichuan University between 2011 and 2020. Receiver operating characteristic (ROC) curves were constructed to calculate the MTC diagnostic efficacy of bCtn and CEA. The differences in the preoperative bCtn and CEA levels of MTC patients with different primary tumor sites and regional lymph node involvement were compared., Results: The bCtn cutoff values were 31.54 pg/mL for men and 22.60 pg/mL for women for diagnosing MTC in the hypercalcitoninemic population with thyroid nodules. There were statistical differences in preoperative bCtn levels ( H =16.166, P =0.001) and in preoperative CEA levels ( H =9.447, P =0.024) in MTC patients of different T stages. There were statistical differences in preoperative bCtn levels ( H =7.919, P =0.019) and in preoperative CEA levels ( H =7.934, P =0.019) in MTC patients of different N stages., Conclusion: The best bCtn cutoff values for the diagnosis of MTC in the hypercalcitoninemic population with thyroid nodules and are 31.54 pg/mL for men and 22.60 pg/mL for women., (Copyright© by Editorial Board of Journal of Sichuan University (Medical Sciences).)

Objectives: To explore the effect and mechanism of Chinese medicine Bushen Huatan formula in treatment of polycystic ovary syndrome (PCOS)., Methods: Twenty-four SPF female C57BL/6J mice were randomly divided into 3 groups with 8 animals in each group. Control group was given drinking water ad libitum ; PCOS was induced by giving letrozole gavage and high-fat diet in model group and treatment group; treatment group received Bushen Huatan formula suspension for 35 d. The sex hormone levels of mice were detected by enzyme-linked immunosorbent assay. Ovary morphology was observed under light microscope after hematoxylin and eosin staining. The feces in the colon of mice were collected, and the gut microbiota was detected by 16S rRNA sequencing. The short chain fatty acids were detected by gas chromatography-mas spectrometry. The expression of peroxisome proliferator activated receptor (PPARγ) was detected by immunohistochemistry. The mRNA expression of mucin-2, occludin-1, tight junction protein zonula occludens 1 ( ZO - 1 ) and PPARγ in intestinal epithelium were detected by realtime RT-PCR. The expression of inducible nitric oxide synthase (iNOS) and PPARγ was detected by Western blotting., Results: Compared with the control group, the body weight, serum levels of follicle stimulating hormone, luteinizing hormone and testosterone in the model group were increased, and serum levels of estradiol were decreased (all P <0.01); the ovarian structure under light microscope was consistent with the characteristics of PCOS. Compared with the model group, the serum levels of sex hormone and ovarian structure in treatment group were improved. The overall structure of gut microbiota in PCOS model mice changed. Compared with control group, there were significantly reduced abundance of Firmicutes , and increased abundance of Verrucomicrobia , Proteobacteria and Actinobacteria inthe model group at phylum level (all P <0.05); there were significantly reduced abundance of Lactobacillus , and increased abundance of Akkermansia , Lachnoclostridium , Lactococcus and Eubacterium&#95;coprostanoligenes at genus level (all P <0.05). The disordered condition of gut microbiota was significantly improved in treatment group. Compared with control group, the contents of acetic acid, propionic acid and butyric acid in feces of model group were significantly decreased (all P <0.05); while the contents of propionic acid and butyric acid in treatment group were significantly increased compared with model control group (both P <0.05). Compared with control group, the mRNA expression of ZO - 1 and protein expression of iNOS in model group were significantly increased, and the protein expression of PPARγ and the mRNA expressions of mucin - 2 and occludin - 1 were significantly decreased (all P <0.05). Compared with model group, the mRNA expression of ZO - 1 and protein expression of iNOS in treatment group were decreased, and the protein expression of PPARγ and the mRNA expressions of mucin-2 and occludin-1 were increased., Conclusions: PCOS induced by letrozole high-fat diet induces microflora imbalance in mice. Chinese medicine Bushen Huatan formula may increase the level of short chain fatty acid by regulating gut microbiota, thereby activating the intestinal PPARγ pathway and improving intestinal barrier function to act as a cure for PCOS.

Objective To investigate the regulatory effect of wingless gene 7a (Wnt7a) on Bacille Calmette Guerin (BCG) induced autophagy in alveolar epithelial cells. Methods The alveolar epithelial cells of TC-1 mice were treated with interfering Wnt7a lentivirus and BCG alone or together in four groups, namely, small interfering RNA control (si-NC) group, si-NC combined with BCG group, Wnt7a small interfering RNA (si-Wnt7a) group, and si-Wnt7a combined with BCG group. The expressions of Wnt7a, microtubule associated protein 1 light chain 3 (LC3), P62, and autophagy related gene 5 (ATG5) were detected by Western blot analysis, and the distribution of LC3 was detected by immunofluorescence cytochemical staining. Results Compared with those in the si-NC group, Wnt7a, ATG5 and LC3 expressions increased and green fluorescent spots of LC3 significantly increased in the BCG infected TC-1 cells; the expressions of Wnt7a, LC3, P62, and ATG5 and green fluorescent spots of LC3 significantly decreased in the si-Wnt7a combined with BCG group comparing with those in the si-NC combined with BCG group. Conclusion Knocking down Wnt7a inhibits BCG induced autophagy in mouse alveolar epithelial cells.

Objective To investigate the regulation of Wnt5a/receptor tyrosine kinase-like orphan receptor 2 (ROR2) signaling pathway on macrophage autophagy induced by Bacille Calmette Guerin (BCG) infection. Methods RAW264.7 cells were infected with BCG at 0, 2, 6, 12 and 24 hours, and the expressions of Wnt5a, ROR2 and autophagy-related protein microtubule-associated protein 1 light chain 3II (LC3II ) were detected by Western blot analysis. After RAW264.7 cells were treated with ROR2 small interfering RNA and BCG infection respectively or together, the protein expressions of autophagy-related genes 5 (ATG5), P62, beclin-1, ATG7 and LC3II in RAW264.7 cells were tested by Western blot analysis. Autophagy flux was detected by mRFP-GFP-LC3 double-label adenovirus assay. Results Compared with the control group, Wnt5a, ROR2 and LC3II had the highest expression in RAW264.7 cells 6 hours after BCG infection. Compared with the non-infected control group, the expressions of autophagy-related proteins ATG5, P62, beclin-1, ATG7 and LC3II showed an increase, along with increased number of autophagosomes and autophagolysosomes in RAW264.7 cells infected with BCG. Compared with BCG infected group, the expressions of the above proteins observed a decrease, and the number of autophagosomes and autophagolysosomes both descended in the co-treatment group with knockdown ROR2 and BCG infection. Conclusion Knockdown of ROR2 can inhibit autophagy in macrophages induced by BCG infection.

In recent years, researchers, pharmaceutical companies, and political makers gradually using more real-world data (RWD) to produce real-world evidence (RWE) for policy-making. A research team of Harvard University launched the RCT DUPLICATE project in 2018, aiming to replicate 30 randomized controlled trials using the medical claims database in order to explore methods for quantifying the efficacy-effectiveness gap and explain its potential sources, to enhance the credibility of the RWE. This paper reviews the background of RCT DUPLICATE Initiative, highlights the research purposes, research design and implementation process of the RCT DUPLICATE Initiative, to help domestic scholars better understand the scope and application value of RWE.

With the promotion and application of big medical data, non-interventional real-world evidence (RWE) has been used by regulators to assess the effectiveness of medical products. This paper briefly introduces the latest progress and research results of the RCT DUPLICATE Initiative launched by the research team of Harvard University in 2018 and summarizes relevant research experience based on the characteristics of China's medical service to provide inspiration and reference for domestic scholars to conduct related RWE research in the future.

Anaplastic lymphoma kinase (ALK) fusion gene is an important tumor driver gene of non-small cell lung cancer, accounting for about 5% of patients with non-small cell lung cancer, of which 97% are patients with lung adenocarcinoma. Since the first discovery of echinoderm microtubule associated protein-like 4 (EML4)-ALK fusion in patients with lung adenocarcinoma in 2007, a variety of ALK fusion partners have been detected. CLIP1-ALK fusion gene was detected by next generation sequencing (NGS) in this patient with advanced lung squamous cell carcinoma, and Alectinib and Ensartinib were taken orally on May 5, 2021. Aletinib was effective for this patient but the patients died on September 30, 2021. This is a report of lung squamous cell carcinoma patients with CLIP1-ALK fusion gene treated with ALK inhibitors.
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Objective: To investigate the clinicopathological features and HER2 expression of metaplastic squamous cell carcinoma (MSCC) of the breast. Methods: A total of 47 MSCC cases diagnosed in the Wuhan Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China from January 2010 to December 2021 were reviewed. The clinical information (including the follow-up data of HER2 positive patients) and pathological features were collected and analyzed. Results: All of the patients were female. Among the 47 cases, 25 were pure squamous cell carcinoma (PSCC) and 22 were mixed metaplastic carcinoma with squamous cell component (MMSC). The median age of the patients was 54 years (range, 29-84 years). The maximum diameter of the mass ranged from 0.8 to 10.0 cm, with a mean value of 3.3 cm, 85.7% (24/28) of the cases were smaller than 5 cm, and only 4 cases were larger than or equal to 5 cm. 89.5% of the MMSC presented with a solid mass. Cystic changes were more commonly found in the PSCC group (50%, P <0.05) than the MMSC group. 36.7% (11/30) of the patients had lymph node metastasis at the time of diagnosis. The squamous cell carcinoma component in all cases showed diffuse or patchy expression of p63, p40 and CK5/6. 55.3% (26/47) of the cases showed triple-negative phenotype. Among the 7 HER2-positive patients, 6 were MMSC group, which had a significantly higher rate of HER2-positivity than that in the PSCC group (1 case). In 1 MMSC case, immunohistochemistry showed HER2 2+in the invasive ductal carcinoma component and HER2 negativity (0) in the squamous cell carcinoma component, but HER2 FISH was negative in invasive ductal carcinoma and positive in squamous cell carcinoma component. Six HER2-positive MSCC patients received anti-HER2-targeted therapy, including two patients who received neoadjuvant chemotherapy combined with anti-HER2-targeted therapy before surgery. One patient achieved pathological complete remission, while the other achieved partial remission (the residual tumors were squamous cell carcinoma components). After 9-26 months of follow-up, four patients had no disease progression, two patients developed pulmonary metastases, and one patient showed local recurrence. Conclusions: MSCC is a group of heterogeneous diseases. PSCC and MMSC may be two different entities. Most of the MSCC are triple-negative and HER2 positivity is more commonly seen in MMSC with invasive ductal carcinoma component. Some HER2-positive MSCC patients can achieve complete remission or long-term progression-free survival after receiving anti-HER2 targeted therapy, but the squamous cell carcinoma component may be less sensitive to targeted therapy than the invasive ductal carcinoma component.

Objectives: To investigate the correlation between stress hyperglycemia ratio (SHR) and outcomes in patients with acute ischemic stroke treated with endovascular treatment. Methods: In a multicenter registration study for RESCUE-RE (a registration study for critical care of acute ischemic stroke after recanalization), eligible patients with large vessel occlusion stroke within 24 hours after onset who received endovascular treatment between July 2018 and May 2019 were enrolled. SHR was calculated as the fasting glucose concentration divided by the estimated average glucose concentration and then categorized into four groups according to the quartiles (group Q 1 , group Q 2 , group Q 3 and group Q 4 ). The primary outcome was poor neurological outcomeat day 90 fromstroke onset [defined as modified Rankin scale (mRS) of 3-6]. Secondary outcomes included early neurological deterioration (END), death within 3 months after stroke onset, and symptomatic intracranial hemorrhage.Multivariable logistic and Cox regression modelswere used to assess the correlation between quartiles of SHR and prognosis in patients with endovascular treatment. Results: A total of 592 patients were enrolled in the study, with a mean age of (63±12) years, and 68.07% were male.The median National Institute of Health stroke scale(NIHSS) score on admission was15(11, 20), and the median SHR was 1.23 (1.07, 1.47), with SHR<1.07 in group Q 1 , 1.07≤SHR<1.23 in group Q 2 , 1.23≤SHR<1.47 in group Q 3 and SHR≥1.47 in group Q 4 , respectively. The rate of complete recanalization was lower in group Q 4 than that of group Q 1 (70.27% vs 83.67%, P =0.026). After fully adjusted for potential covariates, the risk of poor neurological outcome at day 90 from stroke onset in group Q 4 was 2.38 folds that of group Q 1 (adjusted OR = 2.38, 95% CI : 1.57-3.57, P =0.003). The risk of death within 3 months of patients in group Q 4 was 1.80 times that of the patients in group Q 1 , but the difference was not statistically significant(adjusted HR =1.80, 95% CI : 0.90-3.62, P =0.098). Conclusion: Higher SHR was correlated with poor neurological outcome at 3 months in large artery occlusion related acute ischemic stroke patients receiving endovascular therapy.

Objective: To develop a suitable scale for assessing the public health safety literacy in residents in China. Methods: The initial scale of Chinese public health safety literacy was developed through theoretical conceptualization, item pooling, field verifying and item inclusion and exclusion. Then the initial scale was converted into an electronic questionnaire. A total of 2 809 residents from 4 provinces were randomly selected for field testing. Classical test theory (CTT) and item response theory (IRT) were used for item reduction. SPSS 23.0 was used for exploratory factor analysis (EFA) and unidimensional testing. Package R 4.1.1 ltm and mirt were used for the analysis of the psychometric properties of items and generate the ICC, IIC and TIF. Results: The initial scale had 30 items (B1-B30), and the test took 9.8 s to complete one item averagely. According to the CTT, B2 was deleted due to coefficient of total correlation (CITC) <0.3 and the item-dimension correlation coefficient (IDCC) <0.4. B23 was deleted due to CITC<0.3, IDCC<0.4 and difficulty index ( W ) <0.2. B30 was deleted due to CITC<0.3 and W <0.2. The total Cronbach's α of the scale was 0.923 after deletion. EFA indicated that 14 items should be deleted due to lower factor loadings <0.7. EFA was conducted for remaining 13 items and 2 common factors were extracted, the factor loadings of all items were >0.7, the accumulated variance contribution of the 2 common factors was 63.361%, and the total Cronbach's α was 0.891, showing unidimensionality, IRT was used to test the remaining items. B14 and B20 were deleted due to discrimination coefficient ( a ) <0.3, difficulty threshold coefficient ( b ) ∉[-3,3], the small amount of information and the flat, crowded, non-monotonic ICC, and IIC. Finally, the Cronbach's α of the 11-itemed scale was 0.936 with TLI=0.97, CFI=0.99, and RMSEA = 0.03. Conclusion: The final scale has good reliability, validity, discrimination, difficulty level and feasibility, and can be applied for the rapid assessment of public health safety literacy in China.

Objectives: This study aimed to investigate the effect of transfecting SOX2-shRNA vector lentivirus to SACC cell lines on the biological behavior of salivary adenoid cystic carcinoma (SACC)-LM and SACC-83., Methods: Three types of SOX2-shRNA lentiviral vectors (817, 818, and 819) were constructed and transfected successfully. The shRNA with the best inhibitory effect was screened out and transfected into SACC cells. Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) and Western blot were used to detect the expressions of Survivin, E-cadherin, and N-cadherin of SACC-LM and SACC-83. CCK-8 and flow cytometry were used to detect SACC-LM and SACC-83 proliferation and apoptosis. Cell scratch test and Transwell method were used to detect the migration and invasion capabilities of SACC-LM and SACC-83., Results: SOX2-shRNA-819 had the best interference effect among the three lentiviruses. After transfecting SOX2-shRNA-819 into SACC-LM and SACC-83, the expressions of SOX2, Survivin, and N-cadherin were significantly reduced, and that of E-cadherin was significantly increased ( P <0.05). The cell proliferation ability decreased, and the number of apoptotic cells increased ( P <0.05). The cell migration and invasion ability decreased ( P <0.05)., Conclusions: shRNA interference technology reduced SOX2 expression while downregulating Survivin expression. These two expressions may be related. Low SOX2 expression inhibits the proliferation, migration, and invasion of SACC cells and promotes the apoptosis of SACC cells. SOX2 may be involved in the epithelial⁃mesenchymal transition process. This study provided a relevant theoretical basis for targeting SOX2 gene therapy in SACC.

Objective: To characterize a novel HLA allele, A*24:191, its DNA sequence, MHC modeling structure, and the possible influence of the amino-acid residue variations on the molecule., Methods: The HLA sequence was determined by Luminex PCR-SSO and PCR-SBT. Its MHC molecular structure and the possible effects of the amino-acid residue variations were modeled and analyzed with Phyre2, RCSB PDB and HistoCheck software., Results: The PCR-SBT revealed the novel A*24:191 differs from A*24:02 in exon 2 at position 256, 265, 270 with G>C, G>C, A>T. The MHC molecular structure prediction showed that, compared with A*24:02, the 62nd residue of A*24:191 changed from the acidic E to a neutral Q, both with the side chain extending outside the α helix pointing forward the groove, (Risler's score, R=2), the 65th changed from the smaller neutral G extending inside the helix to a basic R with a long-chain extending upward outside the helix (R=52), and the 66th changed from the basic K to a neutral N both with a long side chain extending inside the groove (R=31). The above residues are located on the α helix of the α 1 domain which constituting the side wall of the peptide-binding groove. The DSS Score=3.85. From the surface image of the molecule, it can be clearly seen that the variations of the properties, sizes and configurations of the residues caused significant changes in the shape of the surface structure of the α helix., Conclusion: It suggested that the residue variations are likely to change the peptide binding properties as well as the TCR and antibody binding characteristics of the molecule.

Objective: To study the non/hypo-response to hepatitis B vaccination in HIV-infected patients, identify the influencing factors and provide evidence for the development of hepatitis B prevention and control strategies and measures for special population. Methods: On the basis of the randomized controlled trial of 20 µg hepatitis B vaccine immunization at 0-1-6 month, 0-1-2-6 month and 60 µg hepatitis B vaccine immunization at 0-1-2-6 month, the HIV-infected patients who completed one-month follow-up after the full course vaccination were selected as study subjects. Quantification of antibody to hepatitis B surface antigen (anti-HBs) in serum samples was performed by using chemiluminescent microparticle immunoassay (CMIA) and demographic characteristics, disease history, HIV infection and treatment status of the study subjects were collected. Statistical analysis was conducted by χ 2 test, t test, unconditional logistic regression and interaction analyses. Results: The non/hypo-response rates to hepatitis B vaccination were 34.65% (35/101), 24.49% (24/98) and 10.99% (10/91) in 20 µg group at 0-1-6 month or 0-1-2-6 month and 60 µg group at 0-1-2-6 month ( P <0.001), respectively. Logistic regression analysis showed that after controlling for confounding factors, the risk for non/hypo-response was 0.22 times higher in HIV-infected patients receiving 60 µg hepatitis B vaccine at 0-1-2-6 month than in patients receiving 20 µg hepatitis B vaccine at 0-1-6 month (95% CI : 0.10-0.50), the risk for non/hypo-response was higher in men than in women ( OR =3.65, 95% CI : 1.88-7.07), and the risk for non/hypo-response was 2.64 times higher in those without hepatitis B vaccination history than in those with hepatitis B vaccination history (95% CI : 1.10-6.32). Moreover, there were multiplicative interactions between immunization schedule and gender ( OR =2.49, 95% CI : 1.24-5.00). Conclusion: The non/hypo-response rate to hepatitis B vaccination was significantly lower in HIV-infected patients receiving 60 µg hepatitis B vaccine at 0-1-2-6 month than in those receiving 20 µg hepatitis B vaccine at 0-1-6 month and 0-1-2-6 month. Gender, vaccination schedule and history of hepatitis B vaccination were the influencing factors of the non/hypo-response to hepatitis B vaccination. There was a multiplicative interaction between vaccination schedule and gender, and men receiving 20 µg hepatitis B vaccines had a higher risk for non/hypo-response to hepatitis B vaccination.

Objective: To investigate and monitor the occupational hazards in the Teaching and Research Laboratory (hereinafter referred to as the place) of a university, so as to provide basis for the occupational health work in the university. Methods: November 2014, 46 places in a university were selected by stratified random sampling, and the occupational health risk factors were investigated. Results: Indoor temperature, humidity, sulfur dioxide, nitrogen dioxide, carbon monoxide and carbon dioxide were detected in 21 sites, xylene and hydrofluoric acid were detected in 6 sites, and colony count was detected in 18 sites, the power frequency electric field intensity was measured in 23 places, and the x-ray radiation dose was measured in 4 places. Noise was measured at 21 sites, with 7 sites exceeding the standards accounting for 33.3% (7/21) ; 21 sites were detected for illumination and 10 sites for nonconformity accounting for 47.6% (10/21) ; 10 sites for Microwave Radiation and 3 sites exceeding the standards accounting for 30% (3/10) ; and 25 sites were detected for outdoor air volume and air velocity, the percentage of unqualified was 72% (18/25) in 18 sites, among which the wind velocity was statistically significant in teaching, research and experimental sites ( P =0.010) . Conclusion: The occupational hazards in the teaching and research places of a university should be paid attention to, and the engineering protection and personal protection should be strengthened in the experiment.

Objective: To explore the differences of risk stratification of very high-risk or extreme high-risk atherosclerotic cardiovascular diseases (ASCVD) and the attainment rates of low-density lipoprotein cholesterol (LDL-C) management targets evaluated by three different criteria, and the causal attributions of these differences. Methods: Patients with ASCVD were consecutively enrolled from January 1 to December 31 in 2019, and were evaluated for very high-risk or extreme high-risk and LDL-C goal attainment rates with 2018 American guideline on the management of blood cholesterol (2018AG), 2019 China Cholesterol Education Program (CCEP) Expert Advice for the management of dyslipidemias (2019EA) and 2020 Chinese expert consensus on lipid management of very high-risk ASCVD patients(2020EC), respectively. The causal attributions of the differences in attainment rates were analyzed as well. Results: A total of 1 864 ASCVD patients were included in this study. According to 2018AG, 2019EA and 2020EC, the proportions of the patients with very high-risk or extreme high-risk were 59.4%, 90.7%, and 65.6%, respectively. The absolute LDL-C target attainment rates were 37.2%, 15.7%, and 13.7%, respectively, the differences between each two rates were statistically significant (all P <0.001). As to the differences in attainment rates between 2020EC and 2018AG, 61.5% were due to the different LDL-C goal attainment values and 38.5% were caused by the different risk stratifications, while for the differences between 2020EC and 2019EA attainment rates, different LDL-C goal attainment values were responsible for 13.2%, and different risk stratifications were responsible for 86.8% of the differences. Conclusions: There are significant differences in the proportions and LDL-C attainment rates among the three different criteria for very high-risk or extreme high-risk ASCVD. 2020EC showed a moderate proportion of patients with extreme high-risk, and had the lowest LDL-C attainment rate. The differences between 2020EC and 2018AG are mainly due to the LDL-C target values, and the differences between 2020EC and 2019EA are mainly caused by the risk stratifications.