1. Study on the Physicochemical Properties of Polysaccharide from Polygonatum sibiricum and Its Protective Effect on D-Galactose-Induced Oxidative Damage in Mice
- Author
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Wang LIU, Jinbo BAI, Wangjuan ZHANG, Chunyang LIU, Muzi LI, Jinhe ZHANG, Guobing XU, and Songzi XIE
- Subjects
polygonatum sibiricum ,polysaccharide ,isolation and purification ,d-galactose ,oxidative damage ,Food processing and manufacture ,TP368-456 - Abstract
In this study, a homogeneous polysaccharide fraction was extracted and purified from the rhizome of Polygonatum sibiricum, its physicochemical properties and protective effect against oxidative damage were analyzed, and its potential protective mechanism was initially explored. The homogeneous polysaccharide (PSP) was obtained by water extraction, alcohol precipitation, and separation on DEAE-52 cellulose and Sephadex G-100 columns. The phenol sulfate method, m-hydroxybiphenyl method, high performance gel permeation chromatography and Fourier infrared spectroscopy were used to analyze the physicochemical properties of PSP. The in vivo antioxidant effects of PSP were investigated in a mouse model of D-galactose induced oxidative damage. The results showed that the carbohydrate content of PSP was 94.42%±14.73% and its molecular weight was 5566.41 Da. PSP was also found to contain β-type furan or pyran fructose, as well as longer branched chains with more branches. Compared with the model group, the levels of SOD, GSH-Px and T-AOC significantly increased (P
- Published
- 2023
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