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Objective To investigate the serum expression characteristics of miR-27b-3p and miR-342-3p in type 2 diabetic nephropathy (DN) patients and the diagnostic value for DN. Methods A total of 157 patients with DN were selected and divided into the normal albuminuria group (NA group, n=49), the microalbuminuria group (MA group, n=41) and the macroalbuminuria group (DN group, n=67) according to the ratio of albumin to creatinine (UACR) of urine specimens. Clinical data and the expression of serum miR-27b-3p and miR-342-3p, renal function and urinary fibrosis indicators were detected by real-time quantitative polymerase chain reaction. Blood urea nitrogen (BUN) and blood creatinine (Scr), were detected by automatic biochemical analyzer. Urine type Ⅳ collagen (Ⅳ-C), laminin (LN) and type Ⅲ procollagen peptide (PCⅢ) were detected by double-antibody sandwich enzyme-linked immunosorbent assay. The factors influencing the pathogenesis of DN and the diagnostic value of miR-27b-3p and miR-342-3p for DN were analyzed. Results Compared with the NA group, the levels of HbA1c, UACR, urinary Ⅳ-C, urinary LN and urinary PCⅢ were increased, while the expresson levels of miR-27b-3p and miR-342-3p were decreased in the DN group and the MA group (P＜0.05). Levels of BUN and Scr were increased in the DN group (P＜0.05), and eGFR level decreased (P＜0.05). The higher level of urinary PCⅢ was a risk factor for DN, while the higher levels of miR-27b-3p, miR-342-3p and eGFR were protective factors of DN (P＜0.05). The expressions levels of miR-27b-3p and miR-342-3p were positively correlated with eGFR (r = 0.647, 0.661, P＜0.05) and negatively correlated with urinary PCⅢ (r=-0.739, P＜0.05). The area under the curve for diagnosing DN by combining eGFR, urinary PCⅢ, miR-27b-3p and miR-342-3p was higher than that of eGFR, urinary PCⅢ, miR-27b-3p, miR-342-3p alone (Z=5.631, 5.794, 6.715, 4.577, P＜0.05). Conclusion The decreased expressions of serum miR-27b-3p and miR-342-3p are associated with decreased renal function and DN occurrence in patients with type 2 diabetes, which can be used as biological indicators for the auxiliary diagnosis of DN. [ABSTRACT FROM AUTHOR]

Objective: To explore the effects of microRNA-342-3p/Mg 2+ Mn 2+ -dependent protein phosphatase 1E (miR-342-3p/PPM1E) on the proliferation, migration, and invasion of clear cell renal cell carcinoma (ccRCC) cells., Methods: The gene chips GSE12105, GSE23085, GSE66271, and GSE66270 were searched, and the relationship between miR-342-3p, PPM1E, and the clinical malignant phenotypes of ccRCC was analyzed. ACHN and 769-P cells were transfected with miR-342-3p inhibitor. The effects of miR-342-3p on cell proliferation, migration, and invasion were examined. ACHN cell line with stable and high expression of miR-342-3p was constructed, and the tumorigenicity of the cell line in BALB/c nude mice was observed. The targeted relationship between miR-342-3p and PPM1E was verified by dual-luciferase reporter gene assay. The cells were transfected with miR-342-3p mimic and pcDNA- PPM1E plasmids to observe whether PPM1E could reverse the effects of miR-342-3p overexpression on the proliferation, migration, and invasion of the cells., Results: The expression of miR-342-3p was upregulated in ccRCC, and there were significant differences among patients with tumors of different T stages and G stages and those with different prognoses ( P <0.05). The overall survival in the miR-342-3p high-expression group was significantly shorter than that in the low-expression group ( P <0.05). Compared with those in the miR-NC group, the miR-342-3p level was significantly downregulated in the inhibitor group, and the cell proliferation ability and the numbers of migrating and invading cells were also significantly decreased ( P <0.05). Compared with the miR-NC group, miR-342-3p group had significantly increased volume and mass of tumor tissues and miR-342-3p level, but significantly decreased level of PPM1E mRNA ( P <0.05). The expression of PPM1E was downregulated in ccRCC, and there were significant differences among patients with tumors of different M stages, N stages, and G stages, and different recurrence statuses ( P <0.05). The miR-342-3p could inhibit the expression of PPM1E in a targeted way. Compared with the miR-NC group, the miR-342-3p group had significantly increased cell proliferation ability and increased numbers of migrating and invading cells ( P <0.05). However, PPM1E could reverse the promotion effect of miR-342-3p mimic on ccRCC cells ( P <0.05)., Conclusion: The miR-342-3p can inhibit PPM1E expression in a targeted way, and thus promotes the proliferation, migration, and invasion of ccRCC cells., Competing Interests: 利益冲突　所有作者均声明不存在利益冲突, (© 2024《四川大学学报（医学版）》编辑部 版权所有Copyright ©2024 Editorial Office of Journal of Sichuan University (Medical Sciences).)

Objective: To investigate the expression level of miR-342-3p in peripheral blood mononuclear cells (PBMCs) of patients with rheumatoid arthritis (RA) and its effect on inflammatory response and migration of synovial fibroblasts in RA (RA-FLS)., Methods: PBMCs were collected from 30 healthy individuals and 50 RA patients for detecting the expression of miR-342-3p, and its correlation with the clinical indicators RF, ESR, anti-CCP, hs-CRP, C3, DAS-28, SAS, and SDS was analyzed. In RA-FLS cultures, the effect of transfection of miR-342-3p mimics and inhibitor on TNF- α -induced inflammatory response of the cells was evaluated by detecting the expressions of IL-1β, IL-6, IL-10, and TNF- α using ELISA. CCK8 assay and Transwell assay were used for detecting the changes in cell viability and migration ability of the synovial cells., Results: In RA patients, the expression level of miR-342-3p was significantly lowered in the PBMCs ( P < 0.05) with an area under the ROC curve of 97.53% and showed inverse correlations with RF ( r =-0.321), ESR( r =-0.284), anti-CCP ( r =-0.355), hs-CRP ( r =-0.320), C3 ( r =-0.294), DAS-28 ( r =-0.395), SAS ( r =-0.366), and SDS ( r =-0.397) (all P < 0.05); a low expression of miR-342-3p was strongly associated with elevated levels of anti-CCP, DAS-28, SDS, and SAS (all with a rule support greater than 85%, confidence greater than 88%, and lift greater than 1). In cultured RA-FLS, TNF- α stimulation significantly increased the cell viability ( P < 0.05), upregulated the expressions of IL-1β, IL-6, and TNF- α , and lowered the expression of IL-10 ( P < 0.05). These changes were significantly suppressed by transfection of the cells with miR-342-3p mimics ( P < 0.05) but enhanced by transfection with miR-342-3p inhibitor ( P < 0.05)., Conclusion: The expression of miR-342-3p is decreased in the PBMCs of RA patients. A lowered expression of miR-342-3p contributes to the pathogenesis of RA by promoting inflammatory responses and migration of RA-FLS.

The aim of this paper was to investigate the effect of flavonoids of Sophorae Fructus on the proliferation, migration and invasion of hepatocellular carcinoma cells and analyze the regulatory mechanism of LncRNA FBXL19-AS1/miR-342-3 p pathway. MTT assay and plate cloning assay were used to detect the effect of flavonoids of Sophorae Fructus at different concentrations(1, 5, 10 mg·mL~(-1)) on the proliferation of liver cancer Huh7 cells. The effect of flavonoids of Sophorae Fructus on the migration and invasion of Huh7 cells was examined by Transwell chamber assay. qRT-PCR was used to detect the effect of flavonoids of Sophorae Fructus on the expression levels of FBXL19-AS1 and miR-342-3 p in Huh7 cells. The dual luciferase reporter assay was used to detect whether FBXL19-AS1 targeted at miR-342-3 p. The effect on the inhibition of FBXL19-AS1 expression or FBXL19-AS1 overexpression and then the proliferation, migration and invasion of Huh7 cells were examined by the above methods. Gelatin zymography was used to detect the activities of MMP-2 and MMP-9. The expression levels of cyclinD1, p21, MMP-2 and MMP-9 proteins were detected by Western blot. Flavonoids of Sophorae Fructus significantly inhibited the proliferation, migration and invasion of Huh7 cells(P&lt;0.05), promoted the expression of p21 protein(P&lt;0.05), and inhibited the expressions of cyclinD1, MMP-2 and MMP-9(P&lt;0.05) in a dose-dependent manner, and could reduce the activity of MMP-2 and MMP-9(P&lt;0.05). The expression level of FBXL19-AS1 was significantly decreased in Huh7 cells treated with flavonoids of Sophorae Fructus(P&lt;0.05), whereas the expression level of miR-342-3 p was significantly increased(P&lt;0.05). The dual luciferase reporter assay confirmed that FBXL19-AS1 targeted at the inhibition of miR-342-3 p expression. After inhibiting the expression of FBXL19-AS1, the inhibition rate of cell proliferation was significantly increased(P&lt;0.05), the number of cell clone formation was significantly reduced(P&lt;0.05), the number of migrated cells and the number of invasive cells were significantly decreased(P&lt;0.05), and the expression levels of cyclinD1, MMP-2 and MMP-9 were significantly decreased(P&lt;0.05), the activities of MMP-2 and MMP-9 were significantly reduced(P&lt;0.05), while the expression level of p21 protein was significantly increased(P&lt;0.05). The overexpression of FBXL19-AS1 reversed the inhibitory effect of flavonoids of Sophorae Fructus on the proliferation, migration and invasion of Huh7 cells. Flavonoids of Sophorae Fructus could inhibite the proliferation, migration and invasion of hepatoma cells by regulating LncRNA FBXL19-AS1/miR-342-3 p pathway.