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The yield of soybean is positively correlated with the duration of reproductive growth, which delays the aging of leaves after flowering, enhances their physiological performance, and supports growing plants with heavier grains. Leaf yellowing is one of the distinctive features of plant aging. Studies on leaf yellowing at the late stage of soybean drum grain have rarely been reported. The early yellowing feature of soybean late tympanic leaves was controlled by a single recessive nuclear gene, according to the genetic analysis of the hybrid of the early yellowing mutant ly and wild-type ofc in this study. Using Molecular Marker to Map-Based Cloning, a 2.23 Mb preliminary localization interval on chromosome 19 was obtained. The interval was shortened to 1.75 Mb and contained 219 genes. When this interval was combined with RNA-Seq analysis, 12 candidate genes were detected, including 4 SNP variant genes and 8 differentially expressed genes. The findings of this study provides the framework for the cloning of genes that cause aging and yellowing during the filling later period in soybean. [ABSTRACT FROM AUTHOR]

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Copyright of Bulletin of Botanical Research is the property of Bulletin of Botanical Research Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Acta Agronomica Sinica is the property of Crop Science Society of China and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Agricultural Science & Technology (1008-0864) is the property of Journal of Agricultural Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

【Objective】 Arabidopsis thaliana was a tool for studying the function of plant genes. It was often difficult for new researchers to figure out thousands of molecular markers when mapping genes for positional cloning. 【Method】In this study, 24 genetic markers distributed on five chromosomes of Arabidopsis thaliana were screened out by using 44 molecular markers screened previously. 【Result】These 24 markers were easy to amplify in PCR detection, the experimental condition was relatively consistent, and the electrophoresis bands divisions were good, and it was an ideal molecular marker for map cloning. 【Conclusion】Using these markers, fast cloning system of the Arabidopsis was established, which greatly improved the efficiency of initial positioning, and achieved rapid initial localization of chromosome of Arabidopsis salt sensitive mutants S28, had proved that the method was efficient and practical. [ABSTRACT FROM AUTHOR]

Copyright of China Sciencepaper is the property of China Sciencepaper and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objectives] The heading date in rice is an important agronomic trait that determines the regional adaptability of plants to specific natural environments, growing seasons and yield, which is controlled by polygenes and complicated genetic basis. Vast studies in the past few decades have uncovered a number of flowering-time loci. However, many genes that controlled heading date of rice still should be identified and the regulated pathway awaits in-depth investigations. [Methods] A rice genetically stable heading delay mutant was selected from ‘Nanjing 35’ radiated by 60Co-y radiation. Genetic analysis on the population from the self-cross of F, hybrid of the wild type X the mutant and based on more than 10 000 individual of F2 population from the mutant and the cultivar ‘93-11’. [Results] The mutant displayed heading later than the wild type about 50 days in the normal long-day conditions in Nanjing, even in artificial short-day conditions, also later about 23 days. We found that the heading delay mutant trait was controlled by a single recessive nuclear gene, tentatively designated as OsSET34. OsSET34 was limited in the interval marker W21 and W24 on the long arm of chromosome 9 with the physical range of 45 kb. And, 5 and 1 open reading frames (ORF) in this interval were predicted from the data in Gramene and RAP-DB website database, respectively. According to the genome sequences analysis of the wild type and the mutant, there was a deletion of 3 bases in the 8th exon of ORF1 (SET domain contain protein) in mutant, w-hic-h resulted in the 288th amino acid changing from histidine to asparagine and the next arginine missing, and then led to the change of the function of this gene. [Conclusions] The photoperiodic genes were regulated by OsSET34 gene (SDG724) through histone methylation in heading time regulation of rice. [ABSTRACT FROM AUTHOR]