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Objective To screen a key molecule, leucine-rich glioma inactivated protein 4 (LGI4), which participates in endothelial cell-mediated osteosarcoma metastasis, and to further analyze its function and molecular mechanism in osteosarcoma metastasis. Methods A co-culture model of human umbilical vein endothelial cells (HUVECs)-osteosarcoma cells was established, and the genes with significant fold change were screened by whole transcriptome sequencing in combination with relevant literature. After, the candidate target gene, LGI4 was verified with real-time quantitative PCR (RT-qPCR) and Western blotting, the expression of LGI4 at mRNA and protein levels was also determined. Osteosarcoma cell line 143B and MTF LGI4 were transfected with the overexpression plasmid of LGI4 to construct the cells with stable overexpression of LGI4. Then the experiments included 5 groups of osteosarcoma cells, including negative control group (normally cultured), co-culture group (co-cultured with HUVECs), co-culture medium group (treated with the HUVECs medium), vector control group (transfected with control plasmid) and LGI4 group (overexpression of LGI4). Transwell assay and wound healing test were used to detect the migration and invasion abilities of osteosarcoma cells. A tumor metastasis model in nude mice was used to verify the effect of LGI4 overexpressed osteosarcoma cells on distant metastasis in vivo. Bioinformatics analysis was applied to analyze the potential downstream target signaling pathways of LGI4, and recovery experiments were performed with related inhibitors to clarify the role of target signaling pathways in the regulation of osteosarcoma metastasis by LGI4. Results The expression levels of LGI4 were markedly decreased in 143B and MTF cells after co-culture with HUVECs (P < 0.05). Compared with the negative control group, the cell migration and invasion abilities of the co-culture group and co-medium group were significantly enhanced, whereas the cell migration and invasion rates of the LGI4 overexpression group were significantly lower than that of the vector control group (P < 0.05). The nude mice metastasis model revealed that the metastasis ability of osteosarcoma cells was suppressed with LGI4 overexpression. Further analysis revealed that NOTCH4 signaling pathway was the downstream target signaling pathway of LGI4, and functional recovery experiments confirmed that LGI4 resulted in osteosarcoma metastasis by inhibiting NOTCH4 signaling pathway (P < 0.05). Conclusion Endothelial cells activate NOTCH4 signaling pathway and thus promote tumor metastasis by inhibiting the expression of LGI4 in osteosarcoma cells.

Objective: To determine the roles of axoplasmic transportation during regeneration after facial nerve injury., Method: Facial nucleus injection and isotopic-label tracer with liquid scintillation counting techniques were used to investigate the changes of radioactivity after guinea pig facial nerve compressed., Result: Axoplasmic transportation was observed again at 3 d after facial nerve injury, and the function recovered until 14 d. At 21 d after injury, it showed that transportation curve of radiolabeled protein was similar with that of the normal. There was particular curve in slow axoplasmic transportation that included two wave: W1 and W2. The axons regenerated at a rate of (2.84 +/- 0.12) mm/d in fast transportation while that of slow transportation is (3.16 +/- 0.08) mm/d., Conclusion: It suggests that axoplasmic transport act as a important role during the course of facial nerve regeneration.