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Objective: To analyze the relationship between serum ferritin(SF) level and high sensitive C reactive protein( hs-CRP) in men and the risk of gout., Methods: We chosed 600 male patients diagnosed with gout as gout group, 600 male patients with hyperuricemia were diagnosed as hyperuricemia group, and randomly selected 600 cases of the same period of male health examination as the control group. The detection information of physical examination and related indicators of three groups were collected, such as height, weight, serum ferritin, high sensitive C reactive protein, uric acid( UA), fasting blood glucose( FPG), triglyceride(TG), total cholesterol( TC) and so on., Results: Serum ferritin( SF) higher than that of hyperuricemia group 114. 45 μg/L( P<0. 05)and the control group 76. 02 μg/L( P<0. 05), while the level of hs-CRP in gout patients up to 0. 3 mg/dL, was significantly higher than that 0. 13 mg/dL in hyperuricemia group and 0. 09 mg/dL in control group( all P<0. 05). After adjusting for BMI, TG, TC, FPG and UA five confounding factors, SF was positively correlated with hs-CRP levels in the hyperuricemia group and the gout group, while there was no association between SF and hs-CRP levels in the control group. The multivariate logistic regression analysis showed that SF( ≥ 69. 01 μg/L) had significantly increased risk of HUA, after adjusting for BMI, TG, TC, FPG and UA five confounding factors, the high level of SF( ≥155. 78μg/L) had significantly increased risk of gout, with OR of 2. 678( 95% CI 1. 484-4. 833), and higher levels of hs-CRP( > 0. 9 mg/dL) was also a risk factor of gout, with OR of 3. 104( 95% CI 1. 727-5. 580). However, SF and hs-CRP were not risk factors of hyperuricemia., Conclusion: Serum ferritin level and high sensitive C reactive protein levels are significantly elevated in patients with gout. It is revealed that hs-CRP, SF may be involved in the pathogenesis of gout patients.

Objective: To discuss the biomechanical stability of locked reconstruction plate for internal fixation of transverse and posterior wall acetabular fracture so as to provide a reliable basis for clinical application., Methods: The models of transverse and posterior wall acetabular fracture were established in 16 anti-corrosion acetabular specimens from 8 adult cadavers, which were randomly divided into experimental group and control group ( n =8). Fracture was fixed with 10-hole posterior column locked reconstruction plate in the experimental group, and with 10-hole posterior column reconstruction plate combined with anterior column lag screw and posterior wall screws in the control group. Biomechanical testing machine was used for loading of 5/6 donor body mass the specimen in a speed of 15 N/s; the loading time was calculated and vertical loading.The longitudinal and quadrilateral body displacements of fracture were recorded to compare the biomechanical stability was performed., Results: The quadrilateral body displacement of the experimental group[(1.99±0.32) mm] was greater than that of the control group[(1.75±0.22) mm], but there was no significant difference ( t =-1.735, P =0.105). The longitudinal displacement[(1.56±049) mm] and the displacement of the posterior wall fracture block[(0.86±0.33) mm] in the experimental group were lower than those of the control group[(1.64±0.51) and (1.01±0.35) mm], showing no significant difference between 2 groups ( t =0.293, P =0.772; t =1.516, P =0.154)., Conclusions: For transverse and posterior wall acetabular fracture, application of locked reconstruction plate can provide sufficient biomechanical stability, reduce the risk of screw placement to acetabular joints.

Objective: To analyze the effect of vitamin E( Vit E) and magnesium( Mg) supplementation on stability of erythrocyte and antioxidant capacity in obese rats., Methods: Seventy Wistar rats were randomly divided into five groups. Each group was supplied defferent doses of Vit E and Mg. All rats were sacrificed after 67 weeks. Erythrocyte osmotic fragility, fluidity of erythrocyte membrane, erythrocytic hemolyses, superoxide dismutase( SOD), malondialdehyde( MDA) and glutathione peroxidase( GSH-Px) were detected. Results of each group were analyzed by One-Way ANOVA andanalysis of variance of factorial design., Results: The values of fluorescence polarization( P) and microviscosity( η) of obese model were( 0. 096 ± 0. 024) and( 0. 543 ±0. 179), respectively, which were significantly higher than other groups( P < 0. 05), it could indicate the fluidity of erythrocyte membrane of obese model was lower than other goups. The erythrocytic hemolyses of obese model was( 33. 57 ± 7. 50) %. Compared with other groups, it was increased by 11. 18%, 10%, 11. 4% and 18. 13%, respectively. The level of SOD of obese model was( 97. 54 ± 24. 81) U/mL which was lower than other groups( P < 0. 05). The level of MDA of obese model was( 17. 18 ± 12. 73) nmol/mL which was higher than other groups( P < 0. 05). Compared with normal controls and obese model, the level of GSH-Px of the group which was adding vitamin E was increased by859. 26 U, 955. 42 U, respectively., Conclusion: Both of Vit E and Mg could improve fluidity of erythrocyte membrane and erythrocytic hemolyses of obese rats. Combined use of Vit E and Mg could effectively improve fluidity of erythrocyte membrane. Vit E or Mg can improve the antioxidant capacity in obese rats.

Objective: To investigate the effects of iron overload on the liver tissue and function of maternal rats' offspring., Methods: Forty pregnant rats were randomly divided into four groups, high-dose group, middle-dose group, low-dose group (120, 60 and 30 mg/kg BW) and control group. Iron detran was administered by intraperitoneal injection every other day and the entire trial lasted for 6 weeks. After 6 weeks, the iron serum levels, GOT and GPT of rats' offspring were determined. Histological changes of the liver injury were measured. The expressions of Bcl-2 and Bax in liver were measured by immunohistochemistry method. In addition, MDA, SOD and GSH-Px in liver were assessed by spectrophotometry., Results: After 6 weeks, the level of serum iron in control group was significantly lower than in middle-dose and high-dose group (P < 0.05), and the result of level of liver iron was similar with that. According to the HE staining, it showed that liver cell gradually was damaged with the increasing of accumulation of hepatic iron. The expression of Bcl-2/Bax was significantly higher in control group than in middle-dose and high-dose groups (P < 0.05). The levels of serum GPT and GOT in high-dose group were significantly higher in control group (P < 0.05). The levels of Liver MDA in middle-dose and high-dose groups were both significantly higher than in control group (P < 0.05). Moreover, the levels of liver GSH-Px and SOD in high-dose group were significantly lower than in control group (P < 0.05)., Conclusion: Iron overload in maternal rats may result in excessive iron deposition and oxidative damage of liver tissue and cell function in maternal rats' offspring.

Objective: The purpose of this study was to analysis on the affection of key protein expression by vitamin D3, and to investigate the mechanism of vitamin D3 on contributing to diabetes., Method: Good status U937 cells were cultured by high glucose medium which contained 45 mmol/L D-Glucose, and treated by 1, 25-(OH)(2)-D3. The control groups were U937 cells cultured by high glucose. The whole cell proteins were extracted and separated by 2-dimentional gel electrophoresis (2-DE), differential expression proteins were identified by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS)., Result: There were 30 protein spots whose expression was significantly different between vitamin D-treated and untreated high glucose loaded cells. Six protein spots were identified by MALDI-TOF-MS, three of which, prohibitin, heat shock 70 kD protein 8, and catalase were protection of cell stress proteins, one of which, electron transport flavoprotein, was respiratory chain related proteins, two of which, fumarylacetoacetate hydrolase and triosephosphate isomerase, were cell metabolism related proteins., Conclusion: The differentially expressed proteins of high glucose load human U937 cell line stimulated by vitamin D3 were found. These differential proteins were mainly related to oxidative stress and energy metabolism. These data suggested that 1, 25-(OH)(2)-D3 might regulate cell high glucose load by reduction oxidative stress injury and affection energy metabolism.

Objective: To understand the nutritional level of lactating mothers and infant by detecting the content of the vitamin B1, vitamin B2, vitamin B6, nicotinic of milk of urban and rural areas and to provide the basis for improving vitamin nutritional status of lactating women and their babies., Methods: Totally 92 pairs of breastfeeding healthy mothers and their children in Shandong Province were selected. 34 pairs were in a urban area and 58 pairs were in a rural area. Collect the milk of selected lactating mothers and the urine of the lactating mothers and their children. Detect the content of vitamin B1, vitamin B2, vitamin B6, nicotinic of milk of lactating mothers and the content of vitamin B1, vitamin B2, nicotinic of urine of lactating mothers and their children., Results: The content of vitamin B2, vitamin B6 and niacin of milk of urban lactating mothers were significantly higher than that of rural lactating mothers. The results of detection showed the vitamin B2 of milk of urban lactating mothers was 149.77 microg/100 g, which was significantly higher than that of rural women in 85.09 microg/100 g (P < 0.05). Vitamin B6 and niacin contents were 15.29 microg/100 g, 40.83 microg/100 g, which were also higher than that in rural lactating milk (6.69 microg/100 g and 24.48 microg/100 g) (All values P < 0.05). However, vitamin B1 of milk of urban and rural lactating mothers were 5.54 microg/100 g and 4.80 microg/100 g respectively, which had no significant difference. Urine analysis showed vitamin B2 and niacin of urban mothers and children were significantly higher than that in rural area (P < 0.05). But the level of vitamin B1 of rural children was higher than that of urban children (P < 0.05). There was no significant difference in the vitamin B1 between urban and rural mothers. The insufficient percentages of vitamin B1, vitamin B2. niacin in urban mothers was 23.5%, 32.3% and 17.6%, and that in rural mothers were 29.3%, 82.8% and 53.4%. The deficiency percentage of vitamin B1, vitamin B2, niacin in urban children were 2.9%, 2.9% and 11.8%, and that in rural children were 5.1%, 51.8% and 25.8%., Conclusion: The insufficient percentage of vitamin B1 in urban and rural mothers was high and the content of vitamin B1 of milk was low. While the insufficient percentage of vitamin B2, niacin of rural lactating mothers and children were higher than that of urban mothers and children.

Objective: To investigate vitamin A (VA), vitamin E (VE) and beta-carotene nutritional status and antioxidase level among tuberculosis patients., Methods: Totally 70 tuberculosis patients were randomly selected as the experiment group from Tancheng Tuberculosis Control in 2010. And 70 matched normal persons were selected as the control group. Two groups of people were relative nutrition index test which include body mass index (BMI), hemoglobin (Hb), triglyceride (TG), total cholesterol ( TC) , high-density lipoprotein (HDL), superoxide dismutase (SOD), catalase (CAT), VA, VE and beta-carotene., Results: BMI of the experiment group was 19.13, which was obviously lower than those of the control group which was 21.95 (P<0.05), but Hb of the experiment group was 128.36 g/L which was lower than those of the control group, not with significant statistic differences. For blood lipid level, TG, TC, HDL of the experiment group were 1.54, 4.47 and 1.21 mmol/L respectively, compared with corresponding indexes of the control group which were 1.63, 5.20, 1.30 mmol/L respectively, were all dramatic decline (P<0.05). Antioxidase level contrast between two groups showed that SOD and CAT of the experiment group were 78.20 and 5.24 U/ml respectively, and corresponding indexes of the control group were 83.27 and 9.99 U/ml respectively, so the former was significantly lower than the later (P<0.05); compared with the control group on vitamin level, VA, VE of the experiment group were 0.256 and 1.148 microg/ml respectively which were all lower than the control group which were 0.385 and 1.182 microg/ml (P<0.05), but beta-carotene of the experiment group was 0.048 microg/ml which was slightly lower than 0.051 microg/ml of the control group, not with significant statistic differences., Conclusion: Nutritional status among the tuberculosis patients is quite poor, especially antioxidase and VA, VE and beta-carotene level are significantly lower than normal people.

Objective: To investigate the effect and mechanism of EGCG on the proliferation and invasion of human hepatioma HepG2 cell., Methods: Hepatioma HepG2 cell was treated with EGCG at different concentrations. The effect of EGCG on HepG2 proliferation was examined by CCK-8. The apoptosis of HepG2 treated with EGCG was observed by fluorescence microscopy and FCM via Annexin V-FITC/PI staining. The invasion of HepG2 was detected by Transwell assay. The expression of MMP-2 and VEGF was analyzed by ELISA., Results: HepG2 proliferation was inhibited after treated with EGCG. The IC25 of 24 h and 48 h was 58.19 and 54.19 mg/L. The IC50 of 24 h and 48 h was 133.90 and 78.97 mg/L. The apoptosis of HepG2 was induced significantly after treated with 60 and 135 mg/L EGCG, and the number of cells crossing Matrigel membrane was (28.33+/-7.66) and 0 (P<0.05), and the inhibitory rate of invasion was 69.47% and 100%. The expression of MMP-2 and VEGF decreased significantly., Conclusion: EGCG suppressed the proliferation and invasion of HepG2 with the possible mechanism of inducing apoptosis and down-regulating the expression of MMP-2 and VEGF in HepG2.

Objective: To study the nutritional status and differences in vitamin B1, vitamin B2, and niacin of the urban/rural infants in Shandong Province, and to provide scientific basis for infants nutrition interventions., Methods: 106 urban infants and 290 rural infants were selected from a city in Shandong Province. Forty milliliter urinary was collected from each one, which was adjusted to pH 4-5 with concentrated hydrochloric acid immediately. The concentration of thiamine, riboflavin and niacin in the urine was detected by fluorescence method., Results: The insufficient percentages of vitamin B, vitamin B2 and niacin in urban infants were 1.9%, 8.0% and 9.1%, and that in rural infants were 4.5%, 56.7% and 27.1%. The median concentrations of vitamin B1 in urban and rural infants were 495.00 and 420.56 microg/g respectively, in which the 12-month and 24-month groups in urban were higher than that in rural (P<0.05). The medians of vitamin B2 content in urban and rural infants were 303.07 and 70.88 microg/g, and the content of vitamin B2 in urban infants was higher than that in rural infants in each group (P<0.05). The median concentrations of niacin content in urban and rural infants were 6.31 and 4.22 microg/g, and the niacin content of 6month-, 12 month-, 18 month- and 24 month- groups in urban infants were higher than that in rural infants (P<0.05)., Conclusion: There were significant differences in vitamin B1, B2 and niacin content of infants between urban and rural areas, and the nutriture of urban infants was better than the rural infants. More improvement measures should be given to infants in rural areas for the high proportion of vitamin B, and niacin deficiency.

Objective: Serum 25-hydroxyvitamin D is very important in evaluating the true vitamin D status of humans. This study is to develop a liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay for the quantitative determination of 25-(OH)D3 in human serum., Methods: Protein in serum sample (400 microl) was precipitated by methanol and the supernatant was extracted with n-hexane. 25-(OH) D3 was detected by using multiple reaction monitoring (MRM) at atmospheric pressure chemical ionization (APCI) in the positive ion mode. Column: Waters ACQUITY UPLC BEH C18 (50 mm x 2.1 mm, 1.7 microm). Mobile phase: 100% methanol. Flow rate: 0.2 ml/min. The results of serum 25-hydroxyvitamin D detected by LC-MS/MS assay were compared with radioimmunoassay (RIA, Diasorin)., Results: The limit of quantitation of LC-MS/MS assay was 1.0 ng/ml for 25-(OH) D3 with a linearity in the range of 6-120 ng/ml (r = 0.999). The recovery was 99.5% (range: 97.1% - 103.4%), and RSD was 4.7%. The average value of 25-(OH) D3 of 53 serum samples was 23.69 ng/ml and 20.13 ng/ml for LC-MS/MS and RIA, respectively (P = 0.083). The correlation of the two methods was: RIA = LC-MS/MS-3.57, r = 0.883., Conclusion: LC Tandem MS assay is accurate, convenient and precise at physiologically relevant 25-(OH) D3 concentrations, and the results are comparable well with RIA. This new method can be used for the measurement of 25-(OH)D3 in human serum.

Objective: To study the effect of antioxidant vitamin E (VE), vitamin C (VC) combined with different doses of beta-carotene (beta-C) supplementation on erythrocyte functions in the elderly person., Methods: 300 elderly person at the age from 60 to 75years old were randomized into 5 groups: from group 1 to group 4 were supplemented with (VC 300 + VE 200) mg/d and different doses of beta-C (16.7, 8.4, 5.6 and 0.0 mg/d, respectively), and control group 5 were supplemented with VE 5 mg/d. The trial lasted for 16 weeks. H2O2-caused hemolytic of erythrocytes was measured spectrometricalyl. ATPase activities in erythrocyte membrane were detected by colorimetric analysis. The fluidity of erythrocyte membrane was evaluated by fluorescence polarization (rho) and microviscosity (eta). All the data was analyzed with SPSS12.0., Results: H2O2-caused hemolysis of erythrocyte was decreased significantly from group 1 to group 4 after the trial compared with before the trial (P < 0.01), they also had significant difference in compared with group 5 after the trial (P < 0.01). ATPase activities in erythrocyte membrane were more higher in group 1 than those of group 5 after the trial( P < 0.05). Moreover, rho and eta values were lower from 1 to 4 groups after the trial than itself before the trial (P < 0.01). CONCLUSION; Antioxidant VE, VC combined with different doses of beta-C supplementation could improve the function of erythrocyte in the elderly subjects.

Objective: To estabish a high performance liquid chromatographic (HPLC) method for the determination of dehydroaetic acid in food determinated., Methods: Separation was carried out on a column of C18 (51 microm, 250 x 4.60mm) at the temperature of 25 degrees C with methanol/0.02mol/L ammonium acetate (5:95, V/V) as mobile phase, and elution flow rate was 1.0ml/min. The detection wavelength was 306nm and the injection volume was 10 microl, quantitation was used by external standard method., Results: The results showed a good linear relationship between the peak area and the concentration (r = 0.9997), the recoveries for Dehydroaetic acid products were 96.5% - 99.5%, and the relative standard deviation was 1.09% - 5.85% for different kinds of food., Conclusion: The results showed that the method may be simple, rapid, accurate and applicable for the determination of dehydroaetic acid in food.

Objective: To investigating the effect of vitamin A (VA)at different dosage of vitamin A on anti-oxidative activity and the fluidity of erythrocyte membrane in rats and safe dosage., Methods: Wistar rats selected for VA supplement trial were randomly divided into four groups. The first group was no VA supplement as a control, and the second, third, forth groups were daily supplemented with VA 11.43, 42.86 and 142.86 microg RE/kg respectively. The trial lasted 8 weeks. Vitamin A was dissolved in soybean oil and daily given to animals by gavage. The blood samples were collected at the end of the trial. The level of plasma VA was analyzed by fluorescent spectrometry. SOD, GSH and MDA in plasma were analyzed. The blood erythrocyte membrane fluidity was detected by fluorescence polarization method., Results: The results showed that plasma retinol levels increased with increase of vitamin A. After 8 week supplement, SOD activities in the second and the third groups were lower than those of the first and the fourth group (P < 0.01), which was the highest in the four groups (P < 0.01). The level of plasma MDA in the third was the lowest (P 0.01). GSH-Px activity was higher in the third group than other three groups (P < 0.01). Moreover, P (fluorescence polarization) was lower in 42.86 microg RE/kg group than other three groups, and P significantly increased in the fourth group (P < 0.01), which indicates that the erythrocyte membrane fluidity increased significantly after 42.86 microg RE/kg vitamin A supplement., Conclusion: The supplement of 42.86 microg RE/kg VA could improve anti-oxidative activity and enhance erythrocyte membrane fluidity. Overtake vitamin A could produce a poisoning effect and a stronger oxidative damage.