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Head and neck primary soft tissue sarcoma is a rare adult connective tissue malignant tumor derived from mesenchymal tissue, which can occur in the paranasal sinuses, throat or neck space.The clinical manifestations are local spread masses in the head and neck or difficulty breathing, swallowing, etc al. MRI and enhanced CT examination are the most commonly used to diagnose such diseases. Pathological diagnosis requires immunohistochemistry combined with FISH to detect MDM2 and CDK4. In this report，two cases of primary soft tissue sarcoma were reported,one is parotid high-differentiated liposarcoma and the other is laryngeal dedifferentiated leiomyosarcoma, introducing the characteristics diagnosis and treatment, and reviewing the relevant literature., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Editorial Department of Journal of Clinical Otorhinolaryngology Head and Neck Surgery.)

Objective: To understand the current state and problem of screening and management of chronic kidney disease (CKD) in the community, and to explore the improving strategies., Methods: We established a community-CKD integrated data science platform based on medical information from 79 community health centers, in Xicheng District, Beijing. Patients who referred to 79 community health centers from 21 June 2015 to 20 November 2021 were retrospectively included in this study using the CKD data platform. The monitoring of the indicator of kidney injury, risk factor control, medicine use and device configuration in community were assessed in the study., Results: In the study, 70.6% of the population were identified with high risk of CKD in the total 374 498 individuals who referred to the community health centers. Hypertension (62.3%), coronary heart disease (43.3%) and diabetes (30.4%) were the most common risk factors in high-risk CKD population. Only 17.2% of the patients with high risk of CKD were screened for kidney injury including at least one serum creatine (Scr) or albuminuria test, among which 10 992 (24.2%) individuals were defined as CKD. 22.7% (11 338/49 908) of the total patients with kidney screening in community were defined as CKD, of whom, 42.6% and 46.1% were identified by estimated glomerular filtration rate (eGFR) < 60 mL/(min·1.73 m 2 ) and abnormalities of urinary proteins, respectively. The overall CKD detection rate in the community was 5.2% (19 299/374 498), and the miss-diagnosis rate of CKD was 38.1%. Of the 79 community health centers, 13 (16.5%) were equipped with ACR testing device, and eGFR was reported directly in 66 (83.5%) centers. Altogether 60.3% and 99.7% of the community CKD patients achieved glucose control and blood pressure control, respectively, and 59.3% of the CKD patients who had proteinuria was treated with renin-angiotensin-aldosterone system (RAAS) inhibitors., Conclusion: High-risk CKD population account for a substantial proportion of patients who refer to the community. Early screening, prevention and management of CKD in the community are of great importance to improve the prognosis and decrease the burden of CKD. It's essential to establish a screening and monitoring system, strengthen standardized management and clinician training for improving the ability of CKD management in the community.

Laryngeal schwannoma is a rare clinical benign neurogenic tumor that originated in the larynx. Large laryngeal schwannoma can cause foreign body sensation in the pharynx and swallowing obstruction. Clinical symptoms includehoarseness and dyspnea. The enhanced CT scan illustrates that the localized mass is isolated, with clear boundary with the surrounding tissues in the larynx. A 68-year-old woman presented with a swallowing discorder,endoscopy demonstrated a smooth mass of the left aryepiglottic fold.The patient then underwent endoscopy surgey supported by the low temperature plasma instead of an open incision., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Editorial Department of Journal of Clinical Otorhinolaryngology Head and Neck Surgery.)

As a major factor in determining performances of the electrochemical super capacitor, electrode materials have received wide attentions recently. Bacteria, with their advantages of low cost, abundance, environmental protection and easy to get from nature, have become the promising biomaterials. The composite electrode materials based on bacteria or their related products, have the advantages of large specific surface area, good cycle stability and high capacitance, and become the research focus in the field of super capacitor electrode materials. Herein, the characteristics and related technology of different bacteria on the preparation of electrode materials for super capacitor were summarized. This review systematically elaborates the latest research progress of bacterial composite electrode materials. Moreover, the prospect of super capacitors has been discussed.

Objective: To detect the expression level of ICOS on Th9 cells in mice infected with Schistosoma japonicum , and investigate the relation between ICOS signaling and Th9 cell polarization., Methods: Twenty-five mice with S. japonicum infection were used as models. IL-9 + cells in CD4 + T cells and ICOS + cells in Th9 cells of the mice were detected by flow cytometry 0, 4, 7, 9 weeks and 12 weeks after the infection., Results: Compared with that 0 week after the infection, the proportion of Th9 cells in CD4 + T cells of the mice significantly increased 4, 7, 9, 12 weeks after the infection (all P < 0.05), and the proportion of ICOS + cells in Th9 cells also markedly improved ( P < 0.05)., Conclusions: In S. japonicum infection, the ICOS signaling may have a regulatory effect on Th9 cell polarization.

Objective: To summarize the clinical manifestations, pathological features, diagnosis, and treatment of abdominal solitary fibrous tumor (SFT). Methods: The data of clinical manifestations, pathological features, diagnosis, and treatment of 18 patients with abdominal SFT admitted to Department of General Surgery, Peking Union Medical College Hospital between January 2000 and December 2016 were retrospectively reviewed. Results: Abdominal mass was first presented in 14 patients. The blood routine, liver, kidney, and coagulation function were normal in all the patients. Tumor markers were abnormal in 4 cases. Most SFT showed hypoechoic mass with regular shape under ultrasound. CT findings differed among the cases. All the 18 patients underwent surgical treatment, and the diagnosis was proved by the postoperative histopathology. SFT differed in general morphology. The positive rate of Ki-67, smooth muscle actin (SMA), CD34, and S-100 was high under immunohistochemistry. Conclusions: Early diagnosis of abdominal SFT is difficult, leading to a low preoperative diagnosis rate. Complete removal of the tumor is the only reliable method of radical treatment.

Objective: To study the relationship between the radiotherapy resistance and autophagy. To provide a theoretiacal basis for drugs that regulate autophagy to improve radiotherapy sensitivity., Method: Flow cytometry (FCM) was performed to analyze the distribution of the cell cycle of CNE2 and CNE2/DDP cells under the action of X radiation. The expression of autopagy-specific gene Beclin1 and microtubule-associated protein light chain 3β (MAPLC3β) in CNE2 and CNE2/DDP cells was determined by real time PCR and Immumofluorescence staining., Result: CNE2/DDP and their parental CNE2 cells produced the G2-M phase arrest under the action of X radiation. With the radiation dose increasing,The cells which in the G2-M phase were more and more (P<0. 05). The G2-M phase arrest in CNE2/DDP cells was more obvious than in CNE2 cells (P<0. 05). The expression of Beclin1 and MAPLC3β in CNE2 and CNE2/DDP cells increased under the action of X radiation. What's more, the raise was more and more obvious with the increase of the irradiation dose(P<0. 05). The expression levels of Beclin1 and MAPLC3β in CNE2/DDP was lower than that in CNE2 cells (P<0. 05)., Conclusion: Autophagic cell death may be the one manner of death in nasopharyngeal carcinoma CNE2 and CNE2/DDP cells under the action of X radiation. The radiation resistance of CNE2/DDP cells may be related to the low expression of autophagy-related genes.

Objective: Using methodology of molecular genetics to explore the origin, phylogen, and gene flow of Mycobacterium tuberculosis (MTB) Beijing lineage in the five provinces from northern China, including Heilongjiang, Jilin, Liaoning, Neimenggu and Ningxia., Methods: 234 MTB Beijing lineage strains were genotyped by 24 Variable Number Tandem Repeat (VNTR), and the h (the allelic diversity) value of each VNTR locus was calculated. On individual level of phylogeny, it was constructed Neighbor-Joining (N-J) tree and minimum spanning tree (MST). Phylogenetic tree was built at the population level, and the most recent common ancestor (TMRCA) was estimated through Bayesian model. Molecular variance (AMOVA) was used to understand the gene flow among strains discovered from the five provinces., Results: Allelic diversities of the 24 VNTR loci were low (h: 0.000 - 0.744). 234 strains of MTB Beijing lineage were dispersed in individual branch of the N-J tree, with 62.0% (145) of them grouped to the same "colonial complexes" in MST. At the population level, the evolution relationship of 234 strains appeared the closest to Beijing lineage, which was from MIRU-VNTRplus database, and the bootstrap was 100. The TMRCA was 5308 (95%CI: 4263 - 6470) years. Differences of pairwise Fst values acquired by AMOVA between Jilin and Heilongjiang, Liaoning, Neimenggu and Ningxia, were not statistically significant (P > 0.05)., Conclusion: The genetic similarity of Beijing lineage MTB from the five provinces of northern China was high. The phylogeny branches had no characteristic dispersal in each province. It was speculated that these strains showed an evolution from a clone of MTB Beijing lineage (about 5000 years ago). The gene flow was taking place between neighboring zones.

Objective: To choose the best retraction agent for the clinic by evaluating cytotoxic effects of six chemical retraction agents on human gingival fibroblasts (HGF) in vitro., Methods: HGF were treated with six chemical retraction agents which included 20% Al2(SO4)3, 5% Al2(SO4)3, 15.5% Fe2(SO4)3, 13.3% Fe2(SO4)3, 0.1% HCl-epinephrine and 0.01% HCl-epinephrine. The damage of direct contact and proliferation inhibit were observed by methyl thiazolyl tetrazolium (MTT) colorimetric assay, the ultra structure was observed under transmission electron microscope (TEM)., Results: All of chemical retraction agents caused cell damage and proliferation inhibit. The ability of cytotoxic effect increased in an order of 0.01% HCl-epinephrine<0.1% HCl-epinephrine<5% Al2(SO4)3<20% Al2(SO4)3

A sensitive radioimmunoassay for human plasma arginine vasopressin was established. 125I-sAVP (1369mCi/mg) was first produced by the Iodogen method and could be used for up to one month in -25 degrees C storage. The sensitivity was 0.15 pg/tube. The sensitivity of the assay was 0.60 pg/ml, with a range of 0.6-11.0 pg/ml. The acetone-ethanol petroleum method was used to extract vasopressin from plasma samples, with a recovery rate of 75.6%. The intra- and inter-assay coefficients of variation were 9.8% and 16.8% respectively. Plasma vasopressin extraction was proved to be indispensible to the assay by virtue of its ability to minimize plasma protein interference. The physiological fluctuation of human plasma vasopressin with and without water intake was shown to be significant between normal adults and patients with neurogenic diabetes insipidus.