8 results on '"Luo, Duan"'
Search Results
2. [A study on the antifibrosis effects of pentoxifylline in treating schistosomiasis japonica mice with liver fibrosis].
- Author
-
Xiong LJ, Li SL, and Luo DD
- Subjects
- Animals, Female, Mice, Mice, Inbred Strains, Liver Cirrhosis, Experimental drug therapy, Liver Cirrhosis, Experimental parasitology, Pentoxifylline therapeutic use, Schistosomiasis japonica drug therapy
- Published
- 2008
3. [Study on the effect of heluoshugan capsule on liver fibrosis induced by Schistosoma japonicum infection in mice].
- Author
-
Tong QX, Wu YY, Xu B, and Luo DD
- Subjects
- Animals, Capsules, Collagen Type I metabolism, Collagen Type III metabolism, Drugs, Chinese Herbal therapeutic use, Focal Adhesion Kinase 1 metabolism, Immunohistochemistry, Liver drug effects, Liver metabolism, Liver pathology, Liver Cirrhosis etiology, Materia Medica therapeutic use, Medicine, Chinese Traditional, Mice, Mice, Inbred Strains, Random Allocation, Schistosoma japonicum growth & development, Schistosomiasis japonica complications, Schistosomiasis japonica parasitology, Vascular Endothelial Growth Factor A metabolism, Drugs, Chinese Herbal pharmacology, Liver Cirrhosis drug therapy, Materia Medica pharmacology, Schistosoma japonicum drug effects, Schistosomiasis japonica drug therapy
- Abstract
Objective: To investigate the effect of Chinese traditional medicine heluoshugan capsule on liver fibrosis induced by Schistosoma japonicum infection in mice., Methods: Liver fibrosis in mice was established by Schistosoma japonicum infection in 6 weeks. Suspension of heluoshugan prepared with normal saline was given orally to the mice, 2 capsules for 20 mice daily for 8 weeks. The level of vascular endothelial growth factor (VEGF), focal adhesion kinase (FAK) and type I, III collagen in liver tissue were detected by immuno-histochemistry., Results: The results showed that heluoshugan improved the pathological change of the liver tissue, decreased the level of type I, III collagen, especially type III collagen (P < 0.01). The level of VEGF and FAK expression was inhibited after the administration of heluoshugan, though the level usually increased in liver fibrosis due to the infection., Conclusions: The result suggests that heluoshugan capsule might have therapeutic effect on liver fibrosis induced by the infection of Schistosoma japonicum in mice, by inhibiting the activation of hepatic stellate cells and the pathological change of liver blood vessel.
- Published
- 2006
4. [Expression of hepatic Bcl-2 and Bax proteins in schistosome-infected mice and the role of pentoxifylline].
- Author
-
Wei P, Luo DD, Xiong LJ, and Zeng LL
- Subjects
- Animals, Dose-Response Relationship, Drug, Female, Immunohistochemistry, Liver pathology, Mice, Pentoxifylline administration & dosage, Phosphodiesterase Inhibitors administration & dosage, Random Allocation, Schistosomiasis japonica metabolism, Schistosomiasis japonica pathology, Liver metabolism, Pentoxifylline pharmacology, Phosphodiesterase Inhibitors pharmacology, Proto-Oncogene Proteins c-bcl-2 metabolism, Schistosomiasis japonica drug therapy, bcl-2-Associated X Protein metabolism
- Abstract
Objective: To study the expression of hepatic Bcl-2 and Bax proteins in mice infected with Schistosoma japonicum and the role of pentoxifylline (PTX) in the expression., Methods: Forty mice were randomly divided into 4 groups: one normal control group,mice in the other three groups were all infected each with 25 cercariae, the infected control group was fed for 10 weeks after infection, and 2 weeks after infection, the high dose PTX group was given PTX 360 mg/(kg x d) for 8 weeks and the low dose PTX group was given PTX 180 mg/(kg x d)also for 8 weeks. At the end of 10 weeks all the mice were killed. Bcl-2 and Bax proteins expression was detected by immunohistochemisty., Results: Compared with the normal control group, the expression of Bcl-2 and Bax was significantly higher in the infected control group (P < 0.05). Bcl-2 was significantly higher in high (dose PTX group than in the infected control group and in low dose PTX group (P < 0.05). However there was no significant difference in the expression of Bax among the groups (P > 0.05)., Conclusion: PTX treatment can significantly increase the expression of Bcl-2 in liver tissue of schistosome-infected mice in a dose-dependent manner, and may play a role against liver inflammation and schistosomiasis-related liver fibrosis.
- Published
- 2005
5. [In vitro study on Hep G2 cell infected by hepatitis C virus].
- Author
-
Chen RL, He YW, Gao Y, Li SL, Yang XM, and Luo DD
- Subjects
- Cell Line, Tumor, Hepacivirus genetics, Humans, RNA, Viral genetics, RNA, Viral isolation & purification, Reverse Transcriptase Polymerase Chain Reaction, Hepacivirus growth & development, Virus Replication
- Abstract
Objective: To establish hepatitis C virus (HCV) infected cell model which is similar to the infection in vivo and can support HCV to replicate for a long time., Methods: After infected with HCV-positive serum, Hep G2 cells were cultured for 60 days. Nested RT-PCR was used to detect plus and minus HCV RNA in cultured cells and supernatants., Results: Plus HCV RNA was detected intermittently in Hep G2 cells during 2-30 days, minus HCV RNA was detected during 3-30 days after infection, the detection rate was similar to plus HCV RNA. Plus and minus HCV RNA can be still intermittently detected during 31-60 days after infection. However, the detection rate gradually declined. Plus HCV RNA was also found intermittently positive in the supernatant, and the detection rate was consistent to that in cells. Minus HCV RNA was not detected in the supernatant., Conclusion: Hep G2 cells were susceptible to HCV, and could support HCV to replicate for a relatively long time. Hep G2 is an ideal HCV infection cell model.
- Published
- 2005
6. [Experimental study on enzyme dot assay for detection of hemorrhagic fever with renal syndrome antigen].
- Author
-
Zhang JA, Luo DD, Zeng LL, and Li SL
- Subjects
- Animals, Antibodies, Viral immunology, Early Diagnosis, Female, Fluorescent Antibody Technique, Indirect, Hemorrhagic Fever with Renal Syndrome immunology, Humans, Immunoglobulin G immunology, Male, Rabbits, Rats, Sensitivity and Specificity, Antigens, Viral analysis, Hantaan virus immunology, Hemorrhagic Fever with Renal Syndrome diagnosis, Immunoblotting methods
- Abstract
Objective: To establish a new and efficient method(IEDA) for detection of hemorrhagic fever with renal syndrome virus (HFRSV) antigen., Methods: An immune enzyme dot assay (IEDA) with mixture of three sorts anti-HFRSV-IgG, which was obtained from rabbit vaccinated with EHFV R22, Chen and Hubei strain was employed to detect HFRSV antigen in serum and urine from epidemic hemorrhagic fever (EHF) patients, and compared with indirect immune fluorescence assay (I-IFA), 76 serum samples and 40 urine samples were detected in this study., Results: The results showed that the total positive rate of HFRSV antigen detected by IEDA was 73.68% in serum and 65.00% in urine, while that detected by I-IFA was 75.00% and 70.00%, respectively. The positive rate in primary phase (within 5 days) of HFRSV antigen detected by IEDA was 94.34% in serum and 83.33% in urine, while that detected by I-IFA was 64.42% and 55.56%, respectively, there was significant difference in both serum and urine detections. Correlation study showed a high correlation in the result of IEDA and I-IFA., Conclusion: The results of this study suggested that the IEDA, as compared with I-IFA, was a more specific, sensitive, rapid and simple method with higher positive rate in primary phase. IEDA could be widely used for early diagnosis of HFRS in hospital at grassroots level.
- Published
- 2004
7. [Expression of IL-2 and TNF-alpha in the liver and the effect of injection of these cytokines on liver fibrosis in mice infected with Schistosoma japonicum].
- Author
-
Zeng LL, Luo DD, Li SL, Liu W, and He YW
- Subjects
- Animals, Immunohistochemistry, Interleukin-2 pharmacology, Liver drug effects, Liver Cirrhosis, Experimental parasitology, Male, Mice, Schistosomiasis japonica complications, Tumor Necrosis Factor-alpha pharmacology, Interleukin-2 metabolism, Liver metabolism, Liver Cirrhosis, Experimental prevention & control, Schistosomiasis japonica metabolism, Tumor Necrosis Factor-alpha metabolism
- Abstract
Objective: To detect the expression of IL-2 and TNF-alpha in the liver at different period postinfection of Schistosoma japonicum and their effect on liver fibrosis after supplementary injection of these cytokines., Methods: Mice were infected with schistosome cercariae and divided into 3 groups. Two groups were injected (i.p.) every other day with IL-2 and TNF-alpha respectively for consecutive 4 wk. The third group and an uninfected group of normal mice were regarded as control. The ABC immunohistochemistry and pathologic image multimedia quantification system were applied to detect activity of IL-2 and TNF-alpha., Results: The level of IL-2 and TNF-alpha in the liver in infected but untreated group slowly decreased (from 8, 11, 14 to 18 wk). The supplementary injection of the cytokines at 6 wk postinfection in the two groups increased the cytokines significantly, the level of IL-2 or TNF-alpha was higher at 1-8 wk after the last injection than that of both infected and uninfected control groups (P < 0.01). The granulomatous inflammation and fibrosis in the livers of the two groups were slighter than that of the control., Conclusion: At the 6th wk postinfection with egg deposition, exogenous supplementation with TNF-alpha or IL-2 induces enhanced expression of the two kinds of cytokines, corresponding to a diminished degree of the liver granulomatous inflammation and fibrosis.
- Published
- 2003
8. [Effect of pentoxifylline on the expression of hepatic TGF-beta 1, type I and type III collagen in mice with liver fibrosis due to Schistosoma japonicum infection].
- Author
-
Xiong LJ, Luo DD, Zeng LL, and Li SL
- Subjects
- Animals, Dose-Response Relationship, Drug, Female, Liver metabolism, Liver Cirrhosis, Experimental parasitology, Mice, Pentoxifylline administration & dosage, Phosphodiesterase Inhibitors administration & dosage, Schistosomiasis japonica complications, Transforming Growth Factor beta1, Collagen Type I metabolism, Collagen Type III metabolism, Liver Cirrhosis, Experimental metabolism, Pentoxifylline pharmacology, Phosphodiesterase Inhibitors pharmacology, Schistosomiasis japonica metabolism, Transforming Growth Factor beta metabolism
- Abstract
Objective: To study the effect of pentoxifylline (PTX) on the content of hepatic TGF-beta 1, type I and type III collagen in schistosome-infected mice with liver fibrosis., Methods: Forty mice with schistosomiasis were divided into four groups: one group as control without any treatment, other three were treated with praziquantel 500 mg/(kg.d) for 2 d, high dose PTX 360 mg/(kg.d) for 8 wk, and low dose PTX 180 mg/(kg.d) for 8 wk respectively. Immunohistochemical technique and multimedia color pathographic analysis system were applied to observe the content of hepatic TGF-beta 1, type I and type III collagen in mice infected with S. japonicum before and after treatment., Results: The effect of PTX on the content of hepatic TGF-beta 1, type I and type III collagen in mice was related to the dosage of PTX. High dose PTX treatment significantly reduced the content of TGF-beta 1, type I and type III collagen compared to the control (P < 0.01), whereas no difference was found between the group of low dose PTX treatment and control (P > 0.05)., Conclusion: High dose PTX treatment could reduce the content of hepatic TGF-beta 1, type I and type III collagen significantly in schistosome-infected mice with liver fibrosis.
- Published
- 2002
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.