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Objective To compare three kinds of platelet antibody detection methods used to identify alloantibodies in patients with platelet transfusion refractory(PTR). Methods The 83 samples from PTR patients were analyzed base on three different methods, including solid phase ELISA, Luminex, and capture. The sensitivity, reproducibility, and consistency of different kits were evaluated. Results A total of 71 (62 positive and 9 negative) out of 83 samples showed consistent results by three methods. The consistency between Luminex and solid phase ELISA was 95.2% (Kapp value=0.829, P

Objective To investigate the value of anti-human leukocyte antigen (HLA)antibody level detected by Luminex testing in predicting clinical prognosis of renal transplantation recipients. Methods A total of 1 105 patients scheduled to undergo renal transplantation (354 successfully undergoing renal transplantation)in the 181st Hospital of People's Liberation Army from June 2013 to November 2015 were selected. The serum samples were collected from 1 923 cases before and after renal transplantation. The positive rate and fluorescent intensity of anti-HLA antibody were detected by Luminex testing before and after renal transplantation. The renal function of recipients was also evaluated after renal transplantation. Results Prior to renal transplantation, 51.0%(546/1 071)of serum samples were positive for anti-HLA antibody, including 26.0%(279/1 071)positive for anti-HLA I antibody, 24.9%(267/1 071)positive for anti-HLA I antibody and 11.4% (122/1 071 )positive for both anti-HLA I and anti-HLA II antibodies. Among 354 patients undergoing renal transplantation, 59 (17%)were positive for anti-HLA antibody after renal transplantation, including 25 (4 newly positive after surgery)positive for anti-HLA I antibody, 15 (1 newly positive after surgery)positive for anti-HLAⅡantibody and 19 (4 newly positive after surgery)positive for both anti-HLA I and anti-HLA II antibodies. During subsequent follow-up, 13 patients positive for anti-HLAⅠantibody, 5 positive for anti-HLA i antibody and 1 1 positive for both anti-HLA I and anti-HLA II antibodies developed transplant kidney dysfunction. All patients newly positive for anti-HLA antibody after renal transplantation presented with transplant kidney dysfunction. Conclusions Luminex testing can perform dynamic detection of the positive rate of anti-HLA antibody, which is important in predicting clinical prognosis of recipients after renal transplantation. [ABSTRACT FROM AUTHOR]

【目的】采用液相芯片技术同时定量测定人血清中癌胚抗原(CEA)、甲胎蛋白(AFP)和乙肝表面抗原(HBsAg),并对该方法进行评价。【方法】制备抗体交联微球及生物素标记抗体,用双抗体夹心法对临床血清标本进行测定。【结果】同时检测CEA、AFP和HBsAg时的线性范围分别为0.032～200ng/mL、0.022～55U/mL、0.26～1000ng/mL,最低检测限分别为8.90pg/mL、0.013U/mL、0.24ng/mL,批内变异系数(CV)