1. [Purification and properties of isoflavone-glucosidase].
- Author
-
Xie MJ, Song M, Zou CX, Xu CH, Lu MC, and Jin FX
- Subjects
- Glucosidases metabolism, Hydrogen-Ion Concentration, Temperature, Absidia enzymology, Glucosidases isolation & purification, Isoflavones metabolism
- Abstract
A high activity isoflavone-glucosidase, which hydrolysis glycosides, was obtainde using liquid fermentation from Absidia sp. R strain. The isoflavone-glucosidase was purified 11 folds with yielding rate of 10.9% after ammonium sulfate precipitation and DEAE-Cellocuse (DE-52) ion exchange chromatography. SDS-PAGE results showed that the molecular weight is 53kD. And the optimum temperature, the optimum pH, Km and pI of the enzyme are 50 deegrees C, 5.0, 1.3 x 10(-2) mol/L and 3.2, respectively. The isoflavone-glucosidase is also rather stable under 60 degrees C and in pH range from 5.0 to 7.0. The enzyme can be activated by Co2+ and Ca2+, and be inhibited by Ag+ and Cu2+.
- Published
- 2006