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Objective: To initiate an assessment tool for radiation-induced skin ulcer and reach a standardized assessment for clinical use, so as to contribute to the determination of optimal therapy depending on the assessment. Methods: A questionnaire was designed by reviewing the literatures, referring to the logic of assessment tools of wound diseases including diabetic foot ulcer, pressure injury and lower extremity vascular ulcer, and discussing within the research group. Sixteen experts in wound healing were engaged in the consultation over two rounds of Delphi method. The expert concordance coefficient and expert positive coefficient as well as expert authority coefficient was analyzed by checking the feedback. The reliability of experts' consultation, as well as the coordination and scientificity with assessment tool were analyzed statistically. Then the assessment tool for radiation-induced skin ulcer was established. Results: Thirteen of 16 experts responded over the two rounds consultation. The established assessment tool for radiationinduced skin ulcer contains 2 first-level indicators, 10 second-level indicators and 35 third-level indicators. Within the two rounds of consultation, the positive coefficient were 87.50% and 100% respectively. The expert authority coefficient was 0.937. The Kendall's coefficient of concordance for two rounds consultation were 0.231 and 0.147 respectively (χ2=131.977 and χ2=87.849, P<0.05). Conclusion · Experts showed high enthusiasm toward this research, and had reliable authority. The expert evaluation opinions were well coordinated, thus the results were credible. The established assessment tool for radiation-induced skin ulcer was verified to be scientific and reliable. The tool is easy to be used in clinic. By use of this assessment tool, the therapeutic plan of radiation-induced skin ulcer could be made in a more standardized way [ABSTRACT FROM AUTHOR]

Cancer-associated fibroblasts （CAF） are a key component of the tumor microenvironment, which can secrete a variety of cytokines, chemokines and growth factors, directly and indirectly support cancer cells, also alter the immune cellular environment by inhibiting the activity of immune effector cells and recruiting immunosuppressive cells, thereby allowing cancer cells to evade immune surveillance. CAF has been proven to be associated with the development, progression, and poor prognosis of solid tumors. However, the role of CAF in hematological malignancies is still unclear. This article reviews the research progress of CAF in hematological malignancies.

Objective: To establish a method for qualitative determination of dichloromethane (DCM) in blood by gas chromatography-mass spectrometry (GC-MS) and quantitative determination of DCM in blood by headspace gas chromatography (HS-GC), and to provide reliable support for forensic examination and analysis of poisoning or deaths caused by DCM., Methods: 0.5 mL blood sample was collected, added into headspace vial with chloroform as the internal standard, and processed by heating at 65 °C and evacuation treatment. The intermediate gas in the headspace vial was analyzed by GC-MS for qualitative validation of the method and by HS-GC for quantitative validation of the method. The method was then applied in forensic case analysis., Results: Qualitative validation of the examination method by GC-MS found that the chromatographic peak and mass spectral characteristic ions were specific in samples added with DCM, and that no interference was observed in the blank negative samples. The limit of detection (LOD) was 5 μg/mL. Quantitative method validation by HS-GC found that the chromatographic peak of DCM was well separated from those of eight other volatile compounds, with the resolution>1.5 in all cases; the lower limit of quantification (LOQ) was 20 μg/mL and good linearity was shown within the range of 20 and 1000 μg/mL, R >0.999; the intra-day test precision and inter-day test precision were good (relative standard deviation, or RSD <15% for both) and test accuracy was high (relative error, or δ <15%). With the method established in the study, DCM was detected successfully in the blood of two fatal cases caused by DCM poisoning, with the blood concentration being 470 μg/mL and 915 μg/mL, respectively., Conclusion: This method is shown to be a rapid, stable and accurate approach to the qualitative and quantitative forensic and toxicological analysis of DCM in blood in DCM poisoning cases or deaths caused by DCM., (Copyright© by Editorial Board of Journal of Sichuan University (Medical Sciences).)

Purpose: To measure the density of the cortex in the zygomatic alveolar ridge region of adults by 3D reconstruction, and to analyze the differences of cortical bone density between different vertical facial types in the zygomatic alveolar ridge in different vertical facial types., Methods: Spiral CT scanning data of 48 patients (24 males and 24 females) were chosen for 3D reconstruction and divided into 3 groups(low-angle group, average-angle group and high-angle group) according to the size of the mandibular plane angle (FH-MP), 18 points at which the six planes of the zygomatic alveolar ridge on the left of the upper jaw intersected at 13, 15 and 17 mm above the baseline(the horizontal line of mesial buccal tip of the first maxillary molar) were measured. SPSS 17.0 software package was used for statistical analysis., Results: There was no significant difference between genders, there was significant difference among different vertical bone facial types (P<0.05）, among which the low-angle group [(1331.65±185.70) HU] was the highest and the high-angle group [(1245.62±207.63) HU] was the lowest. There was significant difference in BMD between different sites in different vertical facial types and the sites with the highest BMD in each group were located at 17 mm above on the occlusion plane. Cross-sectional evaluation showed that the high-angle group was located at the level of the proximal and middle buccal root of the first molar, and the mean-angle group was located between the proximal and distal buccal root, and the low-angle group was located at the level of the distal buccal root of the first molar., Conclusions: There was significant difference in density and distribution of bone cortex in zygomatic alveolar ridge between different vertical facial types in adults, which provides a reference basis for adults to select appropriate site of micro-implant anchorage and to ensure the stability of anchorage in orthodontic treatment.

Considering the serious pollution of heavy metal-chromium (Cr) in soil, there is an urgent need for effective selection of Cr-tolerant plant species. In order to gain fundamental insights into the tolerance and accumulation capabilities of Lolium perenne L. and Pharibitis purpurea (L.) Voigt under Cr stress, a pot experiment was conducted to investigate their growth, physiology and accumulation characteristics under Cr(Ⅲ) and Cr(Ⅵ) stress. The results showed the growth parameters could intuitively reflect the toxicity levels of Cr for plants. For instance, a low-level Cr(Ⅲ) (<250 mg·kg -1 ) in soil was good for plant growth as indicated by the significant elevation of plant height, root length and biomass in L. perenne ( P <0.05). However, Cr(Ⅵ) at all concentrations (≥25 mg·kg -1 ) in the soil inhibited the growth of both plant species, and the root length was particularly sensitive to the toxicity of Cr. The physiological parameters of plant represented both the toxicity of Cr and the tolerance of plants under Cr stress. A decrease of root activity and an increase of malonaldehyde content were observed under Cr stress, which indicated the physiological metabolism of plants was disturbed. In the presence of both Cr species, the proline content increased, which served as an indicator for both high Cr toxicity and increase of osmotic balance in plants. A rise in SOD and POD activity reflected the defense ability of plants against oxidative stress caused by Cr. In addition, the Cr-accumulation related parameters were the major standards for tolerant species selection. The Cr(Ⅵ) accumulation capacities of both plant species were greater than their Cr(Ⅲ) accumulation capacities. The maximum accumulation amounts of L. perenne and P. purpurea reached 957.4 mg·kg -1 and 743.3 mg·kg -1 in roots and 394.7 mg·kg -1 and 340.4 mg·kg -1 in shoots, respectively. In comparison with P. purpurea , L. perenne displayed a stronger Cr accumulation capacity in roots with a maximum bioaccumulation factor of 15.55. However, the transport ability of P. purpurea was superior to L. perenne . All of the parameters demonstrated that both L. perenne and P. purpurea could be used as alternative plants for phytoremediation of Cr-contaminated soil.

L9 (3(4)) orthogonal experiment was used to design the extraction technology of compound Clematidis Radix spray. Weight coefficients of active ingredients and dry extract rate were solved by information entropy. Support vector machine (SVM) was established and the model parameters were optimized through the genetic algorithm. Grid search algorithm was used for optimization of extraction technology of Clematidis Radix spray. The optimal extraction technology was to extract Clematidis Radix spray in water with 6 times the weight of herbal medicine for 3 times, with 2 h once. Bias of value between real and predicted by SVM was 1.23%. SVM was compared with traditional intuitive analysis of orthogonal design. It indicates that the new method used to optimize the extraction parameters of compound Clematidis Radix spray is more accurate and reliable.

Combined with the research in an organic farm in the past 10 years, differences of soil aggregates composition, distribution and organic carbon fractions between organic and conventional cultivation were studied by simultaneous sampling analysis. The results showed that the percentages of aggregates (> 1 mm, 1-0.5 mm, 0.5-0.25 mm and < 0.25 mm) in the conventional cultivation were 23.75%, 15.15%, 19.98% and 38.09%, while those in organic cultivation were 9.73%, 18.41%, 24.46% and 43.90%, respectively. The percentage of < 0.25 mm micro-aggregates was significantly higher in organic cultivation than that in conventional cultivation. Organic cultivation increased soil organic carbon (average of 17.95 g x kg(-1)) and total nitrogen contents (average of 1.51 g x kg(-1)). Among the same aggregates in organic cultivation, the average content of heavy organic carbon fraction was significantly higher than that in conventional cultivation. This fraction accumulated in < 0. 25 mm micro-aggregates, which were main storage sites of stable organic carbon. In organic cultivation, the content of labile organic carbon in > 1 mm macro-aggregates was significantly higher than that in conventional cultivation, while no significant difference was found among the other aggregates, indicating that the labile organic carbon was enriched in > 1 mm macro-aggregates. Organic cultivation increased the amounts of organic carbon and its fractions, reduced tillage damage to aggregates, and enhanced the stability of organic carbon. Organic cultivation was therefore beneficial for soil carbon sequestration. The findings of this research may provide theoretical basis for further acceleration of the organic agriculture development.

A four-year field experiment was performed from March 2010 to February 2014 in order to investigate the contribution of different respiratory components to soil respiration and the temperature sensitivity of different respiratory components. Four blocks were arranged in field, and there were trenched and un-trenched plots in each block. Trenching, which can exclude roots, was performed around the trenched plots. A portable soil CO2 fluxes system ( Li-8100) was used to measure soil respiration rates. Soil temperature and soil moisture were simultaneously observed when measuring soil respiration rates. The results showed that the heterotrophic respiration rate in the trenched plots and the soil respiration rate in the un-trenched plots had the same seasonal pattern. Soil respiration rate in the un-trenched plots was significantly (P < 0.001) higher than that in the trenched plots. Mean soil respiration rates in untrenched plots and mean heterotrophic respiration rate in trenched plots were (2.59 ± 0.48 ) and (1.74 ± 0.28) μmol x (M2 x s)(-1), respectively. There was no significant (P > 0.05) difference in the mean soil respiration rate or mean heterotrophic respiration rate between measurement years. The relationship between heterotrophic respiration and soil respiration could be fitted with a proportion function. Heterotrophic and autotrophic respiration contributed 65.9% and 34.1% to the soil respiration, respectively. The main contributor to soil respiration was heterotrophic respiration. The relationship between the ratio of heterotrophic respiration to soil respiration and measurement date could be fitted with a linear function. An exponential function could be used to fit the relationship between heterotrophic respiration and soil temperature, and between autotrophic respiration and soil temperature. The temperature sensitivity coefficient (Q10) for heterotrophic respiration was lower than that for autotrophic respiration.

Purpose: To measure the changes of bonding strength of dentin adhesive agent at different storage temperatures., Methods: One hundred extracted bicuspids were randomly divided into 10 sets, 10 teeth in each set. Each set was further divided randomly into Group A and Group B, each with 5 teeth. During a 10-week test period, Groups A and B were applied with adhesives labeled A and B. Group A was refrigerated under 4 degrees centigrade and B was stored at room-temperature from the second week on. The first set was stored at the same situation. The shear force of samples were tested by universal material tester at the speed of 1 mm/min. The results were evaluated using one-way ANOVA and LSD t test using SPSS 18.0 software package., Results: According to one-way ANOVA, the shear bond strength of the 2 groups differed significantly (P<0.05). Comparing the sets by LSD t test, the results showed that during the first 5 weeks, there was no significant difference between Group A and B. However, there were measurable differences in performance after 6 weeks., Conclusions: With time goes on, the bonding strength of both refrigerated and room-temperature adhesives tend to diminish. After 6 weeks, the bonding performance of the 2 groups begins to differ significantly, and the refrigerated adhesives are superior to room-temperature adhesives.