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In order to explore the economic and carbon reduction benefits of photovoltaic-storage-charging integrated stations and achieve reasonable configuration of internal components, a multi-objective optimization configuration method for stations that takes into account economic and low-carbon aspects is proposed. Firstly, based on the functions and requirements of each module in the charging station, the sources of carbon emissions generated by station is explored, and a mathematical model for the cost and carbon emissions of each module in the station is established. Then, with the goal of minimizing the annual investment and operating cost of the system and carbon emissions, a multi-objective particle swarm optimization algorithm based on three-black-hole capturing strategy is used to optimize the configuration of various modules of stations in different load scenarios, and the optimal configuration plan for each component module of the station under three scenarios is obtained. The comparative results show that the method proposed in this article can effectively reduce the cost and carbon emissions during planning and operation, and improve the economic and environmental benefits of stations. Finally, the technique for order preference by similarity to ideal solution is used to provide a compromise optimization plan for the optimal scenario, which can provide reference for the current investment and construction of photovoltaic-storage-charging integrated stations.

In this study, we aimed to construct a non-replication mRNA platform and explore the side effects of electroporation-mediated delivery of mRNA on the mice as well as the expression features of the mRNA. With luciferase gene as a marker, in vitro transcription with T7 RNA polymerase was carried out for the synthesis of luciferase-expressed mRNA, followed by enzymatic capping and tailing. The mRNA was delivered in vivo by electroporation via an in vivo gene delivery system, and the expression intensity and duration of luciferase in mice were observed via an in vivo imaging system. The results demonstrated that the mRNA transcripts were successfully expressed both in vitro and in vivo . The electroporation-mediated delivery of mRNA had no obvious side effects on the mice. Luciferase was expressed successfully in all the mRNA-transduced mice, while the expression intensity and duration varied among individuals. Overall, the expression level peaked on the first day after electroporation and rapidly declined on the fourth day. This study is of great importance for the construction of non-replication mRNAs and their application in vaccine or antitumor drug development.