Search

Your search keyword '"Liu, Minglong"' showing total 3 results
Start Over Author "Liu, Minglong" Language chinese
3 results on '"Liu, Minglong"'

2. [Preparation of monoclonal antibodies against enterovirus type 71 with an epitope-incorporated adenovirus type 3 vector].

Author

Fan Y, Tian X, Xue C, Liu M, Zhou Z, Li X, Li C, and Zhou R

Subjects
Amino Acid Sequence, Animals, Antibodies, Monoclonal metabolism, Blotting, Western, Capsid Proteins genetics, Cell Line, Tumor, Chlorocebus aethiops, Enterovirus A, Human genetics, Epitopes genetics, Fluorescent Antibody Technique, Genetic Vectors genetics, Humans, Mice, Inbred BALB C, Sequence Homology, Amino Acid, Vero Cells, Adenoviridae genetics, Antibodies, Monoclonal immunology, Enterovirus A, Human immunology, Epitopes immunology
Abstract

Objective To develop the monoclonal antibodies (mAbs) against enterovirus type 71 (EV71). Methods Two neutralization epitopes, SP70 and SP55, from EV71 were cloned into the hexon gene of adenovirus type 3 to generate a recombinant adenovirus type 3 (R1R2A3) presenting SP70 and SP55 antigens. BALB/c mice were immunized with the R1R2A3. The mAbs were developed with hybridoma technology and were analyzed with microneutralizing assay, indirect ELISA, Western blotting and direct immunofluorescence assay (DFA). Results The study obtained four hybridoma cell clones, 2C4, D2C9, I2G2 and I12C3. ELISA showed that the titer of D2C9 against EV71 was 1:8 000 000 and the titers of 2C4, I2G2, and I12C3 all were 1:500 000. ELISA and Western blotting demonstrated that all mAbs could specifically recognize the VP1 of EV71. In addition, D2C9 recognized the SP70 epitope, and 2C4, I12C3 and I2G2 all recognized the SP55 epitope. DFA revealed that all mAbs could react with EV71, but not with Coxsackie virus A16 (CoxA16). Conclusion Four mAbs against EV71 have been developed successfully, and all of them could react with EV71 rather than CoxA16.

Published
2016

3. [Study on effect of fushenkeli on expression of TAK1 in human renal tubular epithelial cells and its possible mechanism].

Author

Liu M, Zeng Y, Lu S, Li J, and Liu T

Subjects
Cell Line, Cell Proliferation drug effects, Collagen Type IV drug effects, Epithelial Cells metabolism, Humans, MAP Kinase Kinase Kinases drug effects, RNA, Messenger drug effects, Transforming Growth Factor beta1 administration & dosage, Collagen Type IV metabolism, Drugs, Chinese Herbal pharmacology, Epithelial Cells drug effects, Kidney Tubules cytology, MAP Kinase Kinase Kinases metabolism, RNA, Messenger metabolism
Abstract

Objective: To investigate the effects of fushenkeli on the expression of TAK1 in the proliferation of the renal tubular epithelial cells induced by TGF-beta1 and its possible mechanism., Method: Human renal tubular epithelial (HK-2) cells were divided into five groups:blank control group, TGF-beta1 group (5 microg x L(-1)), intervention group 1 (5 microg x L(-1) of TGF-beta1 + 100 mg x L(-1) of fushenkeli), intervention group 2 (5 microg x L(-1) of TGF-beta1 + 500 mg x L(-1) of fushenkeli) and intervention group 3 (5 microg x L(-1) of TGF-beta1 + 1 g x L(-1) of fushenkeli). HK-2 proliferation was detected by methyl thiazolyl tetrazolium (MTT) assay. Type IV collagen in the supernatants of the cultured HK-2 was detected by ELISA at 12, 24, 48 hours respectively. The protein and mRNA expressions of TAK1 was measured by Western blot and real-time quantitative PCR., Result: 1) The cell proliferation and the expression of type IV collagen were increased compared with the control group (P<0.05, P<0.01), but they were decreased in intervention group. 2) The expressions of protein and mRNA of TAK1 in TGF-beta1 group were upregulating significantly compared with control group (P<0.01), but they were downregulating in intervention group, especially in intervention group 3., Conclusion: Fushenkeli could inhibits TAK1 expression induced by TGF-beta1 in the proliferation of HK-2 cell.

Published
2011

Catalog

Books, media, physical & digital resources
See catalog results