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The superhydrophobic coating can effectively prevent the intrusion, adhesion and condensation of water-containing corrosive media on the surface. However, the poor mechanical and chemical stability of traditional superhydrophobic coatings significantly limits their application in complex and harsh environments. In this study, a superhydrophobic PDMS-EP@SiO2-GO coating was formed on the surface of Q345 steel sheets by using a composite substrate of graphene oxide(GO) and nano silicon dioxide(SiO2) with polydimethylsiloxane(PDMS) and epoxy resin(EP), combined with spraying technology. The superhydrophobic, self-cleaning, anti-fouling, mechanical wear resistance, chemical corrosion resistance and anti-corrosion properties of the coating were studied. Results showed that the water contact angle of PDMS-EP@SiO2-GO was 166.57°, and the sliding angle was less than 3°. Due to the strengthening effect of GO on the coating substrate, the coating maintained a contact angle of more than 155° for liquids with water temperatures ranging from 20 ℃ to 100 ℃ and a contact angle of more than 160° for acidic/alkaline solutions. The sliding angle was less than 4° under the above conditions. After sandpaper friction(total friction length of 40 m) and 30 cycles of tape-peeling tests, the coating still exhibited good superhydrophobic ability, with the contact angle remaining above 152°. In addition, PDMS-EP@SiO2-GO, with excellent water resistance, demonstrated good anti-fouling and self-cleaning properties. In terms of corrosion protection, the lowest frequency impedance of PDMS-EP@SiO2-GO increased by an order of magnitude, and the charge transfer resistance increased by two orders of magnitude, showing excellent corrosion resistance. PDMS-EP@SiO2-GO addressed the problem of weak mechanical and chemical stability in traditional superhydrophobic coatings and enabled the persistent protection of the metal substrate. The as-prepared superhydrophobic coating, with good practicability, contributes to the safe and reliable service of mechanical equipment throughout its life cycle.

This study investigated the effect of freezing ball milling treatment on the structure, properties and digestibility of waxy rice starch, prepared by alkaline extraction. The effects of treatment time on the particle size distribution, microstructure, crystalline structure, short-range ordered structure, pasting properties, thermodynamic properties and digestibility of waxy rice starch were examined. The results showed that after freezing ball milling treatment, the integrity of starch granules was damaged with cracks appearing on the surface, the particle size tended to increase, and the crystalline structure and short-range ordered structure were severely damaged. The water absorption index increased from 2.15 to 7.31 g/g, and the cold water solubility and swelling power increased significantly from 2.97% and 3.25% to 46.33% and 15.50%, respectively in the 80 min ball milling group compared with untreated samples (P < 0.05). The enthalpy change (ΔH) decreased from 9.33 to 1.79 J/g, and the degree of gelatinization significantly increased (P < 0.05). The birefringence of the starch gradually disappeared with increasing treatment time. The rapidly digestible starch (RDS) content of waxy rice starch increased significantly (P < 0.05), while the contents of resistant starch (RS) and slowly digestible starch (SDS) decreased significantly (P < 0.05). This study provides basic data and references for the production of modified starch products with novel structures and properties.

To investigate the physicochemical properties of edible dock, promote its processing and develop the related products, the safety, advantages as a new food raw material, nutritional components, health efficacy and application status of the edible dock were reviewed, and the prospects for the development of edible dock products were also presented.

A kind of clearance elimination and vibration reduction structure of cylindrical gear slot holes is proposed. The vibration acceleration of slotted and backlash gears with different sizes is simulated using the dynamics software Adams. The vibration acceleration spectrum and power spectrum are obtained by Fourier transform and correlation power spectrum estimation method. A three-factor and three-level orthogonal test table is designed. Taking the amplitude of angular acceleration of meshing frequency as the evaluation index of orthogonal tests, the relatively optimal parameter design scheme is obtained. The effectiveness of the proposed damping structure design is verified by vibration tests. The slot structure can reduce the gear vibration acceleration by about 20%-33%, and the test results are similar to the simulation results. Under the constraint of slot relaxation, the negative backlash has an obvious inhibitory effect on the vibration.

This research aimed to explore the effect of sturgeon head soup, made by high-pressure liquefaction after cooking, in promoting milk secretion in rats with postpartum hypogalactia. Lactating Wistar rats were randomly assigned into blank, bromocriptine-induced hypogalactia, positive control (whey + seabass power), and experimental (sturgeon head soup) groups (n = 6). After 14 days of oral administration, the changes in body mass and milk secretion were investigated. Mammary gland morphology, related hormone levels and protein expression were measured by hematoxylin eosin (HE) staining, immunohistochemistry, enzyme linked immunosorbent assay (ELISA) and real-time fluorescence quantitative polymerase chain reaction (qPCR). The results showed that for the experimental group, an increase in the secretion of proteins that showed a brownish yellow in immunohistochemical analysis was observed in the mammary glandular ducts and acini along with a decrease in the number of adipose connective tissue between the mammary glandular lobules compared with the model group. Milk productivity, mammary gland to body mass ratio, and percent body mass gain of the whole litter of suckling rats were significantly increased in the experimental group (P < 0.05). The levels of prolactin (PRL), prolactin receptor (PRLR), β-casein (β-CN), α-lactalbumin (α-LA), lactose synthase (LS) and fatty acid synthase (FAS) were significantly elevated in the maternal serum and mammary tissue. The mRNA expression of the genes regulating the synthesis of β-CN and FAS in the mammary gland was significantly up-regulated compared with the model group. These results suggest that sturgeon head soup can promote milk secretion in rats with postpartum hypogalactia, and the underlying mechanism may be related to increasing the contents of PRL and PRLR and promoting the expression of β-CN, α-LA and FAS.

As a class of typical metal-organic framework materials(MOFs), actinide-based MOFs feature their unique actinide-ligand bonds. However, how these coordination interactions participate in and affect the lattice assembly process of organic ligands remains less studied. In this work, the coordination assembly of carbazole tricarboxylic acid organic ligands and uranyl ions was taken as a showcase study, and the regulation of uranyl coordination on the assembly process of carbazole tricarboxylic acid in lattice by controlling the synthesis conditions was studied in detail. Single-crystal analyses show that with the introduction of uranyl metal nodes, the assembly of carbazole tricarboxylic acid has realized a gradual evolution from a hydrogen bond-organic framework structure to a metal-organic network. In this regulation process, the connection of hydrogen bonds between different carbazole tricarboxylic acids is gradually replaced by uranyl coordination. The two-dimensional network structure also changes from sextuple intercatenated non-planar network through planar supramolecular network to final coordination network. The related transformation process is mainly manifested in gradual participation of the carboxyl groups in the coordination of uranyl center, which has also been confirmed by infrared spectroscopy. This work reveals the molecular mechanism of the synthesis of inorganic-organic porous materials through uranyl-ligand coordination and the detailed process of material structure evolution.

Leaching of uranium, thorium and rare earth from the monazite selective solution residue were studied by conventional leaching--two-stage countercurrent leaching was studied. The effects of various factors on the leaching was investigated. The results show that the leaching rates of uranium, thorium and rare earth are 98.66 %, 95.37 % and 64.23%, respectively under suitable conditions using conventional leaching method. Using the two-stage countercurrent leaching method, the leaching rates of uranium, thorium and rare earth can be increased by 0.5%~1.0%, the residual acid of the leaching solution can be reduced by more than 50%, and the solid-liquid separation performance of the slurry can be improved to a certain extent. [ABSTRACT FROM AUTHOR]

[Objective] The Late Devonian Frasnian-Famennian (F-F) transition is a critical time interval in geological history. This period saw major simultaneous marine ecological system changes that led to one of the Big Five mass extinctions in the Phanerozoic. The F-F event (also referred to as the Kellwasser event) was characterized by severe losses of low-latitude shallow-water benthic faunas, notably reef-dwelling coral and stromatoporoids. High-latitude, deep-sea and terrestrial faunas were the least affected. Various separate or combined hypotheses have been proposed as the causes of this mass extinction (sea-level change, marine anoxia, climate change, volcanic/hydrothermal activities, and bolide impact) . Of these, climate change and marine anoxia have been the most intensively researched and discussed hypotheses based on the Web of Science in recent years. However, some controversies remain, and the interactions of certain factors are still unclear. [Methods] This study systematically reviews available reports on the paleoclimatic and paleo-oceanic changes during the F-F transition and discusses the anoxic model during this critical period, based on related case studies in southern China. [Results and Conclusions] Conodont oxygen and strontium isotopes, as well as the carbon isotope records for carbonates, collectively suggest a cold climate during the F-F transition interval, with several rapid warming-cooling fluctuations. Conodont oxygen isotopes suggest that the sea-sur face temperature (SST) dropped by 5 ° C-8 ° C. Strontium isotopes also imply that the temperature fluctuations were due to frequent, short-duration volcanism. Palynological data and carbonate platform exposure/karstification are also suggestive of coeval climate cooling. Additionally, marine anoxia has been extensively hypothesized as a possible killing mechanism in the F-F mass extinction, based initially on the presence of bituminous limestones (or black shales), named the lower and upper Kellwasser horizons. Studies of pyrite framboids, biomarker compounds, trace elements, isotopes of nitrogen, sulfur and uranium, and iron speciation have variously suggested the existence of the Kellwasser anoxic events. However, these generally occurred in geographically specific environments notably in the pericontinental basins/sub-basins proximal to source hinterlands. Moreover, the range and degree of anoxia in the F-F transition also reportedly differed between study sections around the world. With regard to the anoxic model, studies have suggested that the Kellwasser anoxic events were caused by the increased nutrient input related to enhanced continental weathering. Marine anoxic studies from three F-F sections comprising different depositional facies in southern China also support this "top down" anoxic model. Clearly, the F-F biotic crisis was not caused by any single factor. Frequent short-term volcanic activity may have enhanced continental weathering and associated greenhouse gas emission, leading to frequent warming-cooling climate fluctuations and also increased oceanic nutrient input. In the latter case, eutrophication and anoxia would have occurred in shallow water, and the mutual interaction of the various environmental factors may have exerted biological pressure in the low-latitude shallow sea, and eventually led to the F-F biotic crisis. [ABSTRACT FROM AUTHOR]

Groundwater reservoir is one of the effective measures for mine groundwater treatment, but mine water has a weakening effect on the mechanical performance of reservoir dam, which threatens the safety and stability of groundwater reservoir.Therefore, it is necessary to carry out the study on the deterioration of rock mechanical properties under the action of mine water.Based on the groundwater reservoir of Lingxin Coal Mine, the deterioration of mechanical properties of gritstone under the effect of mine water immersion was study through laboratory.The test results show that the mass change of the immersed test piece is related to the specific surface area, the interstitial and the rock mineral composition.Under small water pressure, the permeability of gritstone is exponentially attenuated.As the water pressure increases further, the permeability begins to increase as the particles blocking the pores are washed, and the greater the water pressure, the higher the rate of increase of permeability.With the increase of immersion time, the tensile strength of rock has a rapid decrease, then slows down and linearly deteriorates;the uniaxial compressive strength first decreases rapidly, then increases slightly, then decreases.The deterioration of the mechanical properties of gritstone is caused by the infiltration of mine water into the pores of the rock, the ensuing hydration expansion and the dissolution of some minerals in the rock.The increase in uniaxial compressive strength is related to the pore water pressure caused by pore blockage.

To understand the effect of the tunnel section shape on the crack propagation law of surrounding rock in adjacent tunnel under blast loading, detailed research is carried out by model test and extended finite element(XFEM). The experimental study shows that under the blast loading, when the original crack grows through the original crack tip zone of the adjacent tunnel surrounding rock, the circumferential tensile zone resulting from propagating stress wave and the reflection stretch zone under the influence of the adjacent free surface of the tunnel, the growth directions have a great difference. The section shape of the tunnel mainly affects the extension direction of the original crack at the later stages. When the section shape of tunnel is different, the direction of the tensile stress of the free-face reflection tensile wave is also different. Since the direction of the reflection tensile stress is generally perpendicular to the free surface, the original cracks in the surrounding rock of the straight wall archway tunnel and the circular tunnel grow along tangential to the curved free surface, and the approximate “layer crack” phenomenon appears. However, the original crack propagation direction of the surrounding rock of the rectangular tunnel is parallel to the direction of the tensile stress of the vertical plane reflection wave, and the crack propagation is not affected, and it is still penetrated into the free surface along the approximate horizontal direction.

The purpose of this study was to study and analyze the composition and content of routine nutrients, amino acids, fatty acids, and mineral elements in muscle of three cultured populations of Cipangopaludina cathayensis, so as to provide a reference for the development and utilization of Cipangopaludina cathayensis germplasm resources. A total of 180 Cipangopaludina cathayensis with an average weight of (32.12±0.17) g were randomly divided into three groups with 60 Cipangopaludina cathayensis in each group. After shelling, the muscles were taken to test their nutritional components. The results showed that the crude protein, total amino acids, essential amino acids, and delicious amino acids in muscle of Luchuan County population were significantly higher than those of other populations (P<0.05). The amino acid score, chemical score, and essential amino acid index of Liujiang population were the highest. The contentrations of unsaturated fatty acids and polyunsaturated fatty acids in muscle of Luchuan County population were significantly higher than those of other populations, and the atherosclerosis index (IA) and thrombosis index (IT) were lower than those of other populations (P<0.05). The ratio of sigma-n-3/sigma-n-6PUFA of the three populations was in line with the recommended value of FAO/WHO for human diet (0.1~0.2). The contentrations of P and Ca in muscle of Rongshui County population were significantly higher than those of other populations (P<0.05). The study indicates that the muscle protein content of the three cultured populations is higher, the fat content is lower, and the delicious amino acids and unsaturated fatty acids are higher. The nutritional value of the muscle of Luchuan County population are better than those of other populations. [ABSTRACT FROM AUTHOR]

Advances in multi-electron transfer materials and architectured electrodes are two important strategies for innovations in electrochemicalenergy storage. The attainment of both by interfacial electrodeposition of freestanding PPy-PEDOT copolymer films was reported. The composition of the copolymer was verified by FTIR and XPS spectra.The dispersion and microstructure of PPy and PEDOT were studied by EDX mapping and SEM. The capacitive performances of the copolymer films were studied by electrochemical measurements. The results show the copolymer film is composed of PPy and PEDOT with homogeneous distribution in a certain proportion.The SEM images show the film exhibits heterogeneous microstructure and has an open porous 3D network microstructure. Electrochemical characterization shows that the copolymer film is an excellent supercapacitor electrode material with high specific capacitance, good power capability and cycle performance. The multi-electron transfer nature of the copolymer, the copolymerization synergistic effects and the unique microstructure are responsible for the improved charge-discharge performances.

A novel LED driver is proposed and analyzed in this paper. The main circuit topology is based on a quadratic buck and a buck-boost. They share a switch through cascading, simplifying the topology and control strategy, reducing the cost of the control circuit. Using two-stage cascade structure, the input current dead-time problem of the original quadratic buck topology is eliminate. It improves the power factor and the input current THD. At the same time, the duty cycle of the switch operates in a more reasonable area. Finally, the correctness of the theoretical analysis is verified by experiments.

The method of dynamic caustics is adopted to investigate the mechanism of original crack existing in the surrounding rock of adjacent tunnel under the action of blasting load. Conclusions are drawn as follows. Under the action of blasting load, dynamic fraction toughness of pre-crack is not related to the dip angle of pre-crack and the free surface of tunnel. However, the existence of free surface makes pre-crack initiation in advance. In general, the propagation path of pre-crack facing the blasting side of adjacent tunnel under the action of blasting load is related to the propagation path of principal crack from the blast-hole, the free surface of tunnel as well as its section form. Initial propagation path presents approximate vertical upward because of the action of tension wave reflected by the free surface of tunnel. The middle and later periods of pre-crack propagation, the propagation path of principal crack and the stress distribution characteristics of free surface have great effect on the propagation path of pre-crack. The stress distribution characteristics of surrounding rock facing the blasting side are analyzed through the LS-DYNA. From the base angle of side wall to vault facing the blasting side of tunnel, effective stress presents the variation rule of decreasing-increasing-decreasing, of which the maximum points lie to the base angle and the location between sprung arch and vault, the minimum point lie to the side wall. As a result, the pre-crack propagation path is partial to the arch parts and arrest at certain location between the sprung arch and vault.

The air-gap thickness is crucial to the operation of magnetic couplers,and it is of great significance for realizing speed adjustment of couplers through changing air-gap thickness. In order to investigate the relationship between air-gap thickness and adjustable-speed performance of axial-flux solid asynchronous magnetic couplers,a model for axial-flux magnetic couplers is established by combining equivalent magnetic circuit approach and Kirchhoff? s law of magnetic circuit. The magnetic circuit parameters of couplers are solved by utilizing the method of analytical calculation on the above model,then,the torque expression is obtained by integrating three-dimensional correction coefficient and torque formula. Moreover,by synthesizing the torque expression under constant torque load condition,the relationship between air-gap thickness and slip is derived,which is beneficial for acquiring the adjustable-speed relationship of axial-flux solid asynchronous magnetic couplers. The Matlab is used to solve the discretization of torque expression and adjustable-speed relationship,besides,the finite element simulations and experiments are also employed in the contrast and analyses,the accuracy of theoretical calculation is verified and the theoretical foundation for realizing precise speed adjustment of couplers is provided.

The occurrence of coal-bearing strata in China is characterized by the variety of coal-bearing basins，the remarkable spatial and temporal differences of late tectonic deformations.The main controlling factors，which determine the tectonic framework of coalfields，include geodynamic environment，tectonic evolution，deep structures，tectonic stress，and lithologic combination features of coal measures.The continent of China has experienced multi-stage tectonic movements since the late Paleozoic，and due to the spatio-temporal heterogeneity of continental tectonic evolution and the complexity of basement properties，the coalfield structures present complex and ordered patterns.The coalfield tectonic framework of China can be divided into two major coalfield regions，three coal deformation zones，and five coal occurrence structural areas.Taking coal occurrence structural sub-areas as basic units，the tectonic characteristics of five coal occurrence structural areas in China were summarized in this paper.

The advanced treatment of beryllium in wastewater by lime primary precipitation--silica gel adsorption/flocculation sediment--time secondary precipitation method process was studied. The removal effects of silica gel adsorption method and flocculation sedimentation method on trace beryllium in time primary sedimentation titrate were investigated, respectively. The results show that the natural settlement rate and filtration rate of the first time precipitation are fast. The removal of trace beryllium in primary filtrate by flocculation sedimentation method is better than that by silica gel adsorption method. When the dosage of polyaluminum chloride and polyferic sulfate Is 20 g/t and 40 g/t, respectively, the beryllium mass concentration in the threat of time secondary precipitation can reach the discharge standard, and the dosage of polyaluminum chloride sedimentation flocculants is less, and the sedimentation performance is better. The removal rates of uranium, beryllium and fluorine in the wastewater after advanced treatment by time primary precipitation--polyaluminum chloride population sediment--time secondary precipitation method are 99. 99%, 99. 99% and 97. 35%, respectively, which can reach the effluence standard. [ABSTRACT FROM AUTHOR]

Objective: To investigate the expression level of hsa&lowbar;circ&lowbar;0140180 in esophageal squamous cell carcinoma (ESCC) cells, as well as its effect on malignant biological behavior of ESCC cells and the possible molecular mechanisms. Methods: Six pairs of ESCC tissues and corresponding paracarcinoma tissues resected at the Department of Thoracic Surgery, Central Hospital of Nanchong City between November 2018 and March 2019 were collected for whole-transcriptome sequencing, and hsa&lowbar;circ&lowbar;0140180 at a low expression in ESCC tissues was screened out. The TE-1 and KYSE30 cell lines overexpressing hsa&lowbar;circ&lowbar;0140180 were established. qPCR was used to detect the expression of hsa&lowbar;circ&lowbar;0140180 in human normal esophageal epithelial cells and ESCC cells, as well as the expression of miR-1287-5p in TE-1 and KYSE30 cells overexpressing hsa&lowbar;circ&lowbar;0140180. CCK-8 and FCM were used to detect the effect of hsa&lowbar;circ&lowbar;0140180 overexpression on the proliferation and cell cycle of TE-1 and KYSE30 cells. Scratch assay and Transwell assay were used to assess the effects of hsa&lowbar;circ&lowbar;0140180 overexpression on migration and invasion of TE-1 and KYSE30 cells. Dual-luciferase reporter assay was used to confirm the targeting relationship between hsa&lowbar;circ&lowbar;0140180 and miR-1287-5p. WB assay was used to detect the expression of EMT-related proteins and the phosphorylation level of the PI3K-Akt pathway in TE-1 and KYSE30 cells after hsa&lowbar;circ&lowbar;0140180 overexpression. Results: Transcriptome sequencing and qPCR results showed that hsa&lowbar;circ&lowbar;0140180 was lowly expressed in ESCC tissues and cells (P<0.05 or P<0.01) and confirmed its closed-loop molecular signature and localization in the cytoplasm. Overexpression of hsa&lowbar;circ&lowbar;0140180 could significantly inhibit the migration and invasion of ESCC cells (all P<0.05) but had no significant effect on the proliferation and cell cycle. The results of dual-luciferase reporter gene assay demonstrated that hsa&lowbar;circ&lowbar;0140180 targets miR-1287-5p and negatively regulates its expression (P<0.001). Overexpression of hsa&lowbar;circ&lowbar;0140180 could significantly increase the expression of E-cadherin and decrease the expression of Snail (both P<0.05) and the phosphorylation level of the PI3K-Akt pathway (P<0.01, P<0.001) in TE-1 and KYSE30 cells. Conclusion: hsa&lowbar;circ&lowbar;0140180 is lowly expressed in ESCC cells and tissues; it reduces the phosphorylation levels of PI3K-Akt pathway and suppresses EMT process possibly through regulation of miR-1287-5p expression, thus affecting the migration and invasion abilities of ESCC cells. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effects of basonuclin 1 (BNC1) on the proliferation, migration, invasion, cell cycle and apoptosis of esophageal squamous cell carcinoma (ESCC) cells and its mechanism. Methods: TThe mRNA expression level of BNC1 in ESCC cells and normal esophageal epithelial cells was detected by qPCR. The protein expression level of BNC1 in cancer and para-cancerous tissues of 10 ESCC patients was detected by immunohistochemical staining. The expression of BNC1 in KYSE-150 and KYSE-30 cells was down-regulated by siRNA, and the effects of BNC1 on cell proliferation, migration, invasion, cell cycle and apoptosis were investigated using CCK-8, scratch healing, Transwell and flow cytometry assays, respectively. The downstream target genes of BNC1 were identified by ChIP-seq assay and GEPIA online website date analysis combined with knockdown transcriptome sequencing data after BNC1 knockdown. qPCR was used to verify the expression of target genes after BNC1 knockdown, and dual luciferase reporter gene assay was used to confirm the regulatory effect of BNC1 on the target genes. Results:The mRNA and protein levels of BNC1 were higher in ESCC tissues than in para-cancerous tissues (all P<0.01). Knockdown of BNC1 significantly inhibited the proliferation, migration and invasion of KYSE-150 and KYSE-30 cells (P<0.01 or P<0.01), arrested the cells in G1 phase and promoted the cell apoptosis (all P<0.01). The results of ChIP-seq assay and online website GEPIA date analysis combined with at transcriptome sequencing data indicated after BNC1 knockdown that G protein pathway repressor 1 (GPS1) may be a oncogenic target gene that positively regulated by BNC1. The results of qPCR and dual luciferase reporter gene assay showed that BNC1 had a regulatory effect on GPS1 (P<0.01). Conclusion: BNC1 is highly expressed in ESCC tissues and cells. Interfering with BNC1 significantly inhibits the proliferation, migration and invasion of ESCC cells, arrests cells at G1 phase and promotes cell apoptosis possibly by targeting GPS1 to regulate the malignant biological behaviors of ESCC cells. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effects of 1,25(OH)2D3 (1,25-dihydroxy vitamin D3) on the proliferation, migration and cell cycle of esophageal squamous cell carcinoma (ESCC) cells and its mechanism. Methods: Human ESCC cells (TE-1, KYSE30, TE-11 and KYSE510) were treated with different concentrations of 1,25(OH)2D3 and the proliferation was detected by CCK-8. TE-11 and KYSE30 cells were treated with 1,25(OH)2D3 at concentrations of 0, 0.1, 0.15, and 0.2 μmol/L, respectively. Scratch healing test was used to detect cell migration ability, Flow cytometry was used to detect cell cycle distribution, and WB was used to detect the protein expressions of cyclin D1, P27, ERK and p-ERK. Results: 1,25(OH)2D3 significantly inhibited the proliferation of TE-11, KYSE30 cells in a concentration- and time-dependent manner (P<0.05 or P<0.01). As compared with control group, TE-11 and KYSE30 cells that treated with 0.1 and 0.2 μmol/L 1,25(OH)2D3 for 48 h exhibited significantly inhibited migration ability (P<0.05 or P<0.01), increased cell population at G0/G1 phase (P<0.05 or P<0.01), decreased protein expressions of cyclin D1 and p-ERK (P<0.05 or P<0.01), and increased expression of P27 protein (P<0.05 or P<0.01); however, there was no significant difference in total ERK level. Conclusion: 1,25(OH)2D3 can significantly inhibit the proliferation, migration and block the cell cycle of ESCC cells, which may be through the regulation of ERK signal pathway. [ABSTRACT FROM AUTHOR]

In order to enhance the creativity of design of the appearance and package of facial soap products and make the design personalized and irreplaceable, we have discussed this issue based on the visual creativity of graphic elements. Through the analysis of graphic elements, it can be seen that the graphic elements belong to the unique feature description in the packaging design of soap products; in modern packaging design, the graphic creativity used in the appearance packaging of soaps should follow four principles: accuracy, individuation, artistry and limitation; based on the visual creativity of graphic elements, the appearance packaging design of soap products can be realized from the following aspects: graphic isomorphism, positive and negative graphics, variant shadows, text graphics, text deformation, text combination, integration of traditional art and reasonable color use. [ABSTRACT FROM AUTHOR]

BACKGROUND: Zinc ion is a necessary metal trace element in human body, which plays an important role in inhibiting osteoclast activity and promoting osteogenic differentiation. However, the effect of zinc ion at different concentrations on osteogenic differentiation of rabbit bone marrow mesenchymal stem cells has been rarely studied. OBJECTIVE: To evaluate the effects of zinc ion at different concentrations on the proliferation and osteogenic differentiation of rabbit bone marrow mesenchymal stem cells for exploring the appropriate zinc ion concentration. METHODS: Bone marrow mesenchymal stem cells were extracted from the long bone marrow of rabbits and passaged. The cells were divided into six groups, including 10-4, 10-5, 10-6, 10-7 and 10-8 mol/L zinc ion groups, and blank control group. The cell proliferation activity was measured by Cell Counting Kit-8 and the growth curve was drawn. Alkaline Phosphatase Assay kit was used to evaluate the osteogenic differentiation ability of the cells, and the growth activity of the cells was shown by Live-Dead Cell Staining Kit. The mineralization ability of the cells was observed by alizarin red S staining. The expression of osteogenic genes was detected by fluorescence quantitative polymerase chain reaction. RESULTS AND CONCLUSION: The number of cells in each group showed an increasing trend with the prolongation of time except for the 10-4 mol/L zinc ion group. After 5 days of culture, the number of cells was highest in the 10-7 mol/L zinc ion group, but lowest in the 10-4 mol/L zinc ion group. Alkaline phosphatase activity was highest when zinc ion concentration was 10-7 mol/L, but lowest at the 10-4 mol/L zinc ion after cell culture for 14 days. The method of Live-Dead Cell Staining revealed the number of viable cells was highest in the 10-7 mol/L zinc ion group. All cells were almost dead in the 10-4 mol/L zinc ion group. Calcium nodule formation was visible, and the expression level of osteogenic genes was highest in the 10-7 mol/L zinc ion group after 14 days of cell culture. These findings suggest that zinc ion can effectively promote the proliferation and osteogenic differentiation of rabbit bone marrow mesenchymal stem cells in a certain concentration range. The promoting effects of zinc ion on proliferation, osteogenic induction and mineralization are strongest. Therefore, the addition of a suitable concentration of zinc ions in the medium is beneficial for the osteogenic differentiation of rabbit bone marrow mesenchymal stem cells. [ABSTRACT FROM AUTHOR]

Based on the remote sensing image in 1996, 2002 and 2010, with the help of RS and GIS technology, and using the Probit regression model, this paper analyzed the characteristics of land use change in Nanjing City from 1996 to 2010, and the driving factors of land use change. The results showed that the cultivated land and woodland decreased, constructive land, garden plot and grassland continued to increase, and the comprehensive land use change rate was rising since 1996. The results of regression model for cultivated land and woodland change indicated that the change of cultivated land in the years 1996-2002 was mainly affected by the factors of "distance to the nearest rural settlement" and "farmers' population density". However, in the years 2002-2010 it was mainly affected by the factors of "change of per area GDP", "distance to the rural settlement" and "distance to the nearest road". The change of woodland in the years 1996-2002 was mainly affected by the factors of "the elevation" and "distance to the rural settlement". However, in the years 2002-2010 it was mainly affected by the factors of "change of per area GDP", "population density" and "distance to the nearest road". By comparison, the early driving factors of land use change were mainly natural factors, but in recent years, they were mainly social, economic and demographic factors. [ABSTRACT FROM AUTHOR]

For exploring the influence law of explosive stress wave on invisible cracks in back-blast side of tunnel, the test was carried out by adopting the explosion loading transmission type dynamic caustics system. The experimental results show that, under explosion load, back-blast side existing crack defects in the surrounding rock of adjacent tunnel becomes a main disturbance, and perturbation size is connected with distance between the crack and tunnel and crack length. When crack length is 5 mm and the distance between the crack and tunnel respectively is 2,3,4 and 5 mm, the curves of dynamic stress intensity factor in the process of crack propagation present similar laws, namely after the crack run through the roadway, rapid increases to the first peak, and then ranges up and down at a period of time and finally reduce to zero. The difference is that the duration of the oscillation change in the peak fluctuation and the maximum value gradually decreases, the maximum value of are 1.651, 1.572, 1.419 and 1.346 MN/m3/2, which determine that finally extended displacement of invisible crack far-end corresponds for 25. 197, 16. 378, 10. 236 and 5. 984 mm, which renders as the regular of diminishing. The existence of the distance between the crack and tunnel not only has certain delay effect on the propagation of the crack, but also weakened stress wave energy reaching to crack far-end, and the delay and weakened effects gradually are enhanced with the increase of distance between the crack and tunnel. [ABSTRACT FROM AUTHOR]

Building high voltage overhead transmission lines (HVOTL)may incur huge external social cost. This study attempts to estimate such external cost using the Taipower Company's Nantou-Changlin HVOTL as a case study. This HVOTL likely affects the values of farmland. Data were collected from a field survey de- signed from the contingent valuation method (CVM). An econometric model using farmland price as the dependent variable was specified and estimated by two-stage least squares (2SLS) and Heckman two-step model (Heckit). The econometric results show that building this HVOTL would reduce the value of farmland within 600 meters from this line with 23.6 km on both sides. The closer to HVOTL, the lower is the farmland price. The total lost farmland value amounts to 17.1 billion NT dollars. This amount is more than six times the construction cost of 2.7 billion. These research results should be very useful to the Taipower Company and government agencies in assessing the external social cost of the construction of HVOTLs in Taiwan. [ABSTRACT FROM AUTHOR]

The interpolator of constant feedrate is widely used in today's NURBS interpolator, which would cause big chord error and feedrate fluctuation. To solve this problem, this paper proposed two NURBS interpolator methods: the interpolator of controlling chord error and the interpolator for constant material removal rate. The former can confine the chord error in a lower range, which can improve the machining accuracy; the latter can confine the chord error in a much lower range. More important, it can make the material removal rate constant, which can improve the machining quality and increase the service life of the cutter. [ABSTRACT FROM AUTHOR]

Objective: To investigate the changes of nasal ventilation before and after septoplasty by using NOSE scoring scale and nasal function examination, and to explore the correlation between subjective nasal obstruction and nasal function examination and its clinical application value. Methods: A total of 129 cases of nasal septum deviation from December 2021 to April 2023 in our hospital were selected for study. All patients underwent septoplasty. Nasal obstruction symptom evaluation（NOSE） was performed in all patients before surgery and 3 months after surgery. nasal minimal cross-sectional area（MCA） and nasal cavity volume（NCV） were recorded by nasal acoustic reflex, nasal resistance meter and nasal respiration apparatus, nasal resistance（NR）, distance between the nostril to minimum cross-sectional area,（the distance between the nostril to minimum cross-sectional area, MD）, nasal inspiratory volume（IV）, nasal expiratory volume（EV）, the nasal partitioning ratio, NPR includes objective indicators such as inspiratory volume difference ratio（NPRi） and expiratory volume difference ratio（NPRe）. Paired test was used to compare and analyze the changes of various indicators before and after surgery, and the difference（ P <0.05） was statistically significant, and Pearson correlation linear analysis was used to analyze the correlation between subjective and objective indicators. Results: There were statistically significant differences in NOSE score, NCV, NR, MD, EV, IV, NPRe and NPRi of 129 patients before and after surgery（ P <0.05）, while there was no statistically significant difference between MCA before and after surgery（ P >0.05）. Preoperative NOSE score was correlated with NR, NCV, EV, IV, NPRe and NPRi（ P <0.05）, but not with MD and MCA（ P >0.05）. There was correlation between NOSE score and NR, MCA, NCV, EV, IV, NPRe and NPRi（ P <0.05）, but no correlation between nose score and MD（ P >0.05）. Conclusion: The subjective NOSE scale combined with nasal function test has certain clinical reference value in evaluating the surgical effect of patients with deviated nasal septum., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Editorial Department of Journal of Clinical Otorhinolaryngology Head and Neck Surgery.)

Objectives: To explore the value of sympathetic skin response (SSR) in the early diagnosis and prognostic evaluation of Guillain-Barre syndrome (GBS) in children., Methods: A retrospective analysis was conducted on the clinical data of 25 children with GBS who were diagnosed from October 2018 to November 2022, and 30 children who were diagnosed with Tourette's syndrome during the same period were selected as the control group. The characteristics of SSR were compared between the two groups, and the association of SSR with autonomic dysfunction (AD), disease severity, and prognosis was analyzed., Results: The GBS group had a significantly higher abnormal rate of SSR than the control group during the acute phase ( P <0.001). SSR combined with early nerve conduction (within 2 weeks after onset) had a sensitivity of 84%, a specificity of 100%, and an accuracy of 93% in the diagnosis of GBS. There were no significant differences in the proportion of AD cases, as well as the Hughes scores during the disease peak, between the abnormal and normal SSR groups ( P >0.05). All 7 children with poor short-term prognosis (at 1 month after onset) had abnormal SSR., Conclusions: SSR can be used for the early diagnosis of GBS and the monitoring of treatment response in children.

Methanol has become an attractive substrate for the biomanufacturing industry due to its abundant supply and low cost. The biotransformation of methanol to value-added chemicals using microbial cell factories has the advantages of green process, mild conditions and diversified products. These advantages may expand the product chain based on methanol and alleviate the current problem of biomanufacturing, which is competing with people for food. Elucidating the pathways involving methanol oxidation, formaldehyde assimilation and dissimilation in different natural methylotrophs is essential for subsequent genetic engineering modification, and is more conducive to the construction of novel non-natural methylotrophs. This review discusses the current status of research on methanol metabolic pathways in methylotrophs, and presents recent advances and challenges in natural and synthetic methylotrophs and their applications in methanol bioconversion.

Ligustrum lucidum is a woody perennial plant of genus Ligustrum in family Oleaceae. Its dried fruit has high medicinal value. In this study, the authors evaluated the variability and species identification efficiency of three specific DAN barcodes(rbcL-accD, ycf1a, ycf1b) and four general DAN barcodes(matK, rbcL, trnH-psbA, ITS2) for a rapid and accurate molecular identification of Ligustrum species. The results revealed that matK, rbcL, trnH-psbA, ITS2 and ycf1a were inefficient for identifying the Ligustrum species, and a large number of insertions and deletions were observed in rbcL-accD sequence, which was thus unsuitable for development as specific barcode. The ycf1b-2 barcode had DNA barcoding gap and high success rate of PCR amplification and DNA sequencing, which was the most suitable DNA barcode for L. lucidum identification and achieved an accurate result. In addition, to optimize the DNA extraction experiment, the authors extracted and analyzed the DNA of the exocarp, mesocarp, endocarp and seed of L. lucidum fruit. It was found that seed was the most effective part for DNA extraction, where DNAs of high concentration and quality were obtained, meeting the needs of species identification. In this study, the experimental method for DNA extraction of L. lucidum was optimized, and the seed was determined as the optimal part for DNA extraction and ycf1b-2 was the specific DNA barcode for L. lucidum identification. This study laid a foundation for the market regulation of L. lucidum.

Objective: To explore the expression level of exosome derived miR-181b-5p in different disease stages of children with acute lymphoblastic leukemia and its relationship with clinical characteristics., Methods: Bone marrow plasma samples of 86 children with ALL were collected. Exosomes were extracted by exosome extraction kit, and RNA in exosomes was extracted by TRIzol method. The levels of miR-181b-5p in the blood plasma exosomes of the patients in the newly diagnosed group, relapse group, remission group and control group were detected by qRT- PCR. The difference of miR-181b-5p expression level in each group was compared and analyzed, and the relationship between miR-181b-5p expression level and clinical characteristics was analyzed., Results: The expression level of exosomal miR-181b-5p in the newly diagnosed group and the relapsed group was significantly lower than that in the remission group and the control group ( P < 0.05). The expression level of exosomal miR-181b-5p in T-ALL children was higher than that in B-ALL children ( P <0.05). The expression level of plasma exosomal miR-181b-5p in male children was higher than that in female children ( P <0.01)., Conclusion: Exosome derived miR-181b-5p changes dynamically in the course of ALL children, and can be used as a marker miRNA to monitor disease status. Exosomes can transmit information in the tumor microenvironment and serve as a potential carrier for biomolecular targeted therapy.

A standard chronology (STD) was established with Pinus tabuliformis samples collected from Nanwutai at the northern slopes of the Qinling Mountains. The correlations between radial growth of P. tabuliformis and climatic factors were explored. The results showed that radial growth was positively correlated with moisture factors in previous September and current May. The radial growth was positively correlated with temperature factors in pre-vious November and negatively with that in previous October and current May. There was significant lag effect of climatic factors on the radial growth. The radial growth of P. tabuliformis was well correlated with the PDSI drought severity index, with positive correlations being identified from September to December of the previous year and in May of the current year. The regression model was able to simulate the relationship between STD and PDSI. The formations of extremely wide and narrow tree-ring were mainly a result of drought condition. Combined with climatic factors, PDSI could better reflect the radial growth characteristics of P. tabuliformis .

The simultaneous quantitative investigation of aerosol ground observation data and particle concentration data is important for a better understanding of the vertical distribution characteristics of air pollution and formulating reasonable air pollution control measures in Chang'an, Xi'an. CE-318 measurements from October 2018 to April 2021 were systematically analyzed to reveal the seasonal and yearly variations in atmospheric aerosols in Chang'an. Then, the relationship between AOD and particle concentration in different seasons and different pollution degrees was explored. The obtained results were as follows:① the seasonal variation in AOD in Chang'an was determined, whereby autumn (1.02)>winter (1.00)>summer (0.63)>spring (0.47). Distinct monthly and inter-annual differences in AOD were observed, showing that the annual average of AOD in 2019 was higher than that in 2020. ② Obvious seasonal and monthly differences in aerosol main control modes were observed; the dominant mode of aerosols gradually changed from coarse mode to fine mode from spring to winter. The seasonal variation in the main control mode of aerosols in 2019 was similar to that in the whole observation period. The seasonal aerosol Angstrom wavelength index (Angstrom) was evenly distributed in 2020, and aerosol particles existed in coarse mode form. Generally speaking, the aerosol type in Chang'an was mostly mixed aerosol throughout the whole observation period. ③ Significant seasonal variations in the relationship between AOD and Angstrom was shown, in which the air pollution in spring was dominated by coarse mode aerosol particles. The local pollution in summer was caused by coarse and fine mode aerosol particles, and the fine mode particles were dominant when the pollution was obvious. The characteristic distribution of Angstrom in autumn and winter was similar; in case of local pollution, the coarse mode aerosol particles were dominant, and in case of obvious pollution, the fine mode aerosol particles were dominant. ④ The monthly concentration variation trend of PM 2.5 and PM 10 in Chang'an consistent with the maximum and minimum values appeared in January and summer, respectively. Seasonal variation in the concentration of PM 2.5 and PM 10 was apparent, with the highest values in winter. ⑤ A positive correlation between AOD and particulate matter concentration in different seasons and pollution levels was obtained during the observation periods, but the correlation coefficient differed. The specific characteristics were as follows:the correlation between AOD and PM 2.5 concentration was greater than that with PM 10 concentration; the correlation between AOD and particle concentration in autumn and winter was greater than that in spring and summer; and the correlation between AOD and particulate matter concentration was greater in polluted weather. Particle concentration was the most important determinant of AOD change, followed by relative humidity, which was the meteorological factor with the highest interpretation rate of AOD change in Chang'an. This indicates that a higher correlation between AOD and particle concentration would benefit from a reasonable humidity correction of AOD.

Objective: To explore effect of lentivirus-mediated complement C3 silencing vector on the osteogenic ability of human B lymphocyte Raji-osteoblast cell line MG63 co-culture system and its mechanism., Methods: The lentiviral complement C3 silencing vector was constructed and transfected into human B lymphocyte Raji to establish in vitro Raji-osteoblast cell line MG63 co-culture system. The cells were divided into blank control group (without special treatment), complement C3 silencing group (lentiviral complement C3 silencing vector transfection co-culture system) and model group(lentiviral vector transfection co-culture system). The expression of complement C3 in each group was detected by reverse transcription-polymerase chain reaction(RT-PCR) and Western-Blot at 24 h after culture, proliferation of MG63 cells was detected by CCK-8 at 0, 3, 6, 12, 24, 48 and 72 h, apoptosis of MG63 cells in each group was detected by flow cytometry at 24 h after culture, and alkaline phosphatase(AKP) activity of MG63 cells in each group was detected by AKP detection kit, and osteoprotegerin (OPG ) protein expression of MG63 cells in each group was detected by Western-Blot method., Results: RT-PCR results showed that the expression level of C3 in complement C3 silencing group was lower than that in blank control group and model group, Western-Blot results showed that expression of C3 in complement C3 silencing group was lower than that in blank control group and model group, CCK-8 results showed that there was no difference in proliferation ability of MG63 among complement C3 silencing group and blank control group and model group at 3 and 6 h after culture;at 12 h after culture, proliferation ability of MG63 cells with C3 silencing was higher than that of blank control group and model group;at 24, 48 and 72 h after culture, proliferation ability of MG63 cell line with complement C3 silencing group were higher than that of blank control group and model group. Flow cytometry resluts showed that apoptosis of proliferation ability of MG63 cell line with complement C3 silencing group was lower than that of blank control group and model group;AKP detection kit suggested that AKP activity in complement C3 silencing group was higher than that in blank control group and model group, Western-Blot results showed that expression level of OPG protein in complement C3 silencing groupwas higher than that in blank control groupand model group., Conclusion: Silencing of complement C3 could enhance bone formation ability of osteoblast MG63, and it takes time to accumulate this ability. Complement C3 may affect osteogenesis by altering OPG / RANKL / RANK axis.

Objective: To investigate the clinical features, distribution of pathogenic bacteria, and drug resistance of bloodstream infection in children with acute leukemia., Methods: Clinical data of 93 blood culture-positive children with acute leukemia from January 2015 to December 2019 in Department of Pediatrics, The Second Hospital of Anhui Medical University were analyzed retrospectively., Results: In these 93 cases, 78 cases were in the period of neutrophil deficiency. There were 54 Gram-negative bacteria (G - ) (58.1%) found through blood culture, and the top 4 strains were Escherichia coli (15.1%), Klebsiella pneumoniae (13.9%), Pseudomonas aeruginosa (6.5%), and Enterobacter cloacae (6.5%). There were 39 Gram-positive bacteria (G + ) (41.9%) detected, and the top 4 strains were Staphylococcus epidermidis (10.8%), Streptococcus pneumoniae (6.5%), Staphylococcus hemolyticus (5.4%), and Staphylococcus human (5.4%). Among 74 strains of pathogenic bacteria from acute lymphoblastic leukemia (ALL) children, there were 29 strains of G + bacteria (39.2%) and 45 strains of G - bacteria (60.8%). While in 19 strains from acute myeloblastic leukemia (AML) patients, G - bacteria accounted for 47.4% and G + bacteria accounted for 52.6%. In 15 ALL children without neutropenia, G + bacteria made up the majority of the strains (66.7%). In the 93 strains of pathogenic bacteria, 13 (13.9%) strains were multidrug-resistant. Among them, extended-spectrum β-lactamases accounted for 42.9%, carbapenemase-resistant enzyme Klebsiella pneumoniae 15.4%, and carbapenemase-resistant enzyme Enterobacter cloacae strains 33.3%, which were detected from G - bacteria. While, 13.3% of methicillin-resistant coagulase-negative Staphylococci accounted for 13.3% detected from G + bacteria, but linezolid, vancomycin, teicoplanin Staphylococcus and Enterococcus resistant were not found. The average procalcitonin (PCT) value of G - bacteria infection was (11.02±20.282) ng/ml, while in G + infection it was (1.81±4.911) ng/ml, the difference was statistically significant (P<0.05). The mean value of C-reactive protein (CRP) in G - infection was (76.33±69.946) mg/L, and that in G + infection was (38.34±57.951) mg/L. The prognosis of active treatment was good, and only one case died of septic shock complicated with disseminated intravascular coagulation (DIC) and gastrointestinal bleeding caused by carbapenemase-resistant enzyme enterobacteriaceae., Conclusion: G - is the major bacteria in acute leukemia children with bloodstream infection, but the distribution of ALL and AML strains is different. G - bacteria dominates in ALL, while G + bacteria and G - bacteria are equally distributed in AML. Non-agranulocytosis accompanied by bloodstream infections is dominant by G + bacteria. The mean value of PCT and CRP are significantly higher in G - bacteria infection than in G + bacteria.

A 3D printing based wrist orthosis device was developed. After collecting the contour information of the carpal and metacarpophalangeal joints of the patients with a 3D scanner, the wrist orthotics were designed to meet the individual needs of the patients according to the relevant requirements of biomechanics. Choose TPU (thermoplastic polyurethanes) materials for preparation of 3D printing. It can functionally assist the smart brace after stroke patients with hemiplegia early rehabilitation training, the use of orthoses carry MPU6050 inertial sensor, magnetometer, time module device such as a sensor and monitor its movements and record the training time, ensure safe efficient rehabilitation training, help patients return to a normal life as soon as possible.

Objective: To explore the influencing factors in children with chronicity immune thrombocytopenia (ITP), and to provide basis for judging the prognosis and treatment in children with ITP., Methods: The clinical data of children with ITP admitted to The Second Affiliated Hospital of Anhui Medical University in the past 5 years were retrospectively analyzed and followed up for more than 1 year. According to the inclusion criteria, the eligible cases (328 cases in total) were selected and collected through medical record system retrieval, outpatient clinic and telephone follow-up. Independent influencing factors affecting the prognosis of children with ITP were obtained through single-factor and multi-factor logistic analysis, and their predictive value for the prognosis of ITP in children were evaluated., Results: Of 328 children with ITP, 208 were newly diagnosed with ITP (64%), 54 were persistent ITP (16%), 66 were chronic ITP (20%), and the remission rate within 1 year was 79.9%. The results of univariate analysis showed that, age, pre-morbidity history of infection and vaccination, antinuclear antibodies, initial absolute lymphocyte count（ALC） and treatment options were related to the prognosis of the children (P<0.05). Multivariate analysis showed that the history of infection and vaccination before onset, initial treatment options, and ALC at the time of initial diagnosis were independent factors affecting the prognosis of children with ITP (P<0.05). The time for platelet recovery to 100×10 9 /L in the initial treatment group combined with intravenous immunoglobulin (IVIG) was shorter than that in the single corticosteroids group (P<0.01). The receivers operating characteristic (ROC) was drawn with the development of chronic disease (course >12 months) as state variable and ALC or ALC combined with preceding infection or vaccination history as test variable. The results showed that when the absolute value of lymphocytes was 3.80×10 9 /L, the area under the curve was the largest (0.787), the sensitivity was 80.6%, and the specificity was 65.53% (P<0.01), the combined results showed that the maximum area under the curve was 0.859, the sensitivity was 77.61%, and the specificity was 78.41%., Conclusion: The initial treatment plan combined with IVIG can reduce the occurrence of chronicity in children with ITP, and its efficacy is better than that of the single corticosteroids group (the platelet recovery time is shorter); history of preceding infection or vaccination, ALC at the time of initial diagnosis are independent factors affecting the prognosis of children with ITP, and the combination of the two shows a better predictive value for the prognosis.

Objective: To study the risk factors and infection characteristics of nosocomial infection in children with acute lymphoblastic leukemia (ALL) and analyze the relationship between different nutritional status and nosocomial infection, early treatment response., Method: The clinical data of 133 children with ALL treated with CCCG-ALL-2015 from June 2016 to June 2019 (chemotherapy stage, risk level, MRD), infection during hospitalization (course of infection, laboratory indicators, sites of infection, outcome) and nutritional status (sex, age, height/ length, weight) were enrolled. The Chi 2 test and Logistic regression analysis were used for statistical analysis., Results: The rate of nosocomial infection was 19.9% in 133 children with ALL, in which 3 were infection-related death. Sex, immunophenotype and risk showed no significantly affect on the occurrence of nosocomial infection (P＞0.05), but neutrophil count, hemoglobin level, platelet count, chemotherapy stage, length of stay in hospital and nutritional status showed affect on the occurrence of nosocomial infection (P＜0.05). Logistic multivariate regression analysis showed that chemotherapy stage, length of hospital stay, neutrophils and nutritional status were the independent risk factors, in which the respiratory tract infection was the most common. Gram-positive bacteria, Gram-negative bacteria and fungi accounted for 44.1%, 52.9% and 2.9% respectively. The negative rate of MRD in day 19 and day 46 between different nutritional status groups showed statistically significant (P＜0.05)., Conclusion: Neutrophil count, chemotherapy stage, length of stay in hospital and nutritional status are independent risk factors for nosocomial infection. Among of them, nutritional status negatively correlated with nosocomial infection, and the poorer nutritional status, the higher risk of nosocomial infection. Malnutrition, overweight and obesity can affect the early treatment response of ALL children. The level of nutrition at first diagnosis can be used as a bad factor to evaluate the early treatment response of ALL children.

Objective: To explore the related factors of fever of unknown causes before operation of intertrochanteric fracture of femur in the elderly., Methods: From August 2015 to August 2018, 156 cases of intertrochanteric fracture of femur were treated by intramedullary nail fixation. According to the preoperative measurement, whether there was fever was divided into fever group and non fever group. There were 80 cases in fever group, 26 males and 54 females, aged 60 to 93 (75.063±13.082) years; 76 cases in non fever group, 39 males and 37 females, aged 60 to 96 (74.763±13.692) years. All patients' sex, age, basic diseases, WBC, NE%, Hb, CRP, D dimer and ALB were observed for single factor analysisand multi factor analysis., Results: Single factor analysis showed that gender, Hb, CRP had influence on preoperative fever, but other indexes had no influence; multi factor analysis showed that gender and CRP might have influence on fever. The probability of fever increased by 1.2%( P =0.050) for every increase of CRP, and there was no significant difference between female and male in the probability of fever ( P =0.061)., Conclusion: CRP is an independent risk factor of preoperative fever in the elderly patients with intertrochanteric fracture of femur. The abnormality of CRP indicates that patients are more likely to have preoperative fever. Perioperative management should pay attention to the monitoring of CRP and early intervention.

We used tree rings of Pinus tabuliformis sampled in the Muwang National Forest Park to establish a standardized chronology (STD) and calculated the correlation coefficients between the standardized chronology and climatic factors of Zhen'an meteorological station. With linear regression analysis, we reconstructed the March-April mean maximum temperature of Zhen'an over 165 years from 1853 to 2017. The highest correlation coefficient was observed between the standardized chronology and the March-April mean maximum temperature (r=0.596, n=60, P＜0.01). The variance interpretation of the March-April mean maximum temperature reconstruction function was 33.2%, and the reconstruction function and results were credible and reliable. Warm years occurred 25 times and cold years occurred 29 times in the reconstruction sequence. The warm years were more accompanied by flood events, while the cold years were accompanied by more drought events. Temperature fluctuated obviously in the reconstruction sequence, with two cold periods (1902-1917 and 1953-2000) and four warm periods (1868-1892, 1917-1937, 1941-1953 and 2001-2012). The obvious periodic variations of 2-7, 8-15, 18-28, 75-96, and 100-125 years were found in the reconstruction sequence, in which the quasi-113, 88 and 22 years were the first, second and third main periods, respectively. These variations might potentially be the fingerprints of some climate change forces such as solar activity, monsoon and EI Niño-Southern Oscillation (ENSO) activity.

Objective: To evaluate the efficacy and safety of 0.9-ms 1064-nm Nd:YAG laser alone or combined with itraconazole for treatment of toenail onychomycosis., Methods: A total of 37 patients with onychomycosis (178 toenails) were randomly assigned to groups A and B, and each group was further divided into different subgroups according to the Scoring Clinical Index of Onychomycosis (SCIO) and Onychomycosis Severity Index (OSI) scoring. All the patients were treated with 0.9-ms Nd:YAG laser once a week for 8 times. The patients in group A were treated with laser alone, and those in group B were treated with laser combined with itraconazole. The clinical effect, clinical scores, appearance of the toenails and adverse reactions in the two groups were analyzed, and the patients' satisfaction rate was also investigated., Results: At the 12th months of follow-up, the clinical response rate and mycological cure rate in group A were 31.33% and 30.00%, respectively, similar to the rates in group B (35.79% and 41.18%, respectively) (P>0.05). After the treatments, the SCIO and OSI scores showed no significant changes in group A (P>0.05) but both increased significantly in group B (P<0.05). The response rates did not differ significantly among the subgroups with SCIO<12 or with OSI<16 (P>0.05), but showed significant differences among the subgroups with SCIO≥12 or with OSI≥16 (P<0.05). Of the total of 178 toenails, 33.71%, 74.72% and 70.79% toenails showed improvements in terms of clear nail growth, shape and color, respectively. The overall patients' satisfaction rate was 62.16%, and no adverse reactions related with the therapy were recorded in these patients., Conclusion: For treatment of toenail onychomycosis, 0.9-ms 1064-nm Nd:YAG laser can effectively improve the aesthetic appearance of the toenails, and a combined treatment with Nd:YAG laser and itraconazole can be better option in severe cases of onychomycosis.

Objective: To explore clinical characteristics and outcome of deep vein thrombosis(DVT) in children with acute lymphoblastic leukemia (ALL)., Methods: A tatol of 266 patients were diagnosed as ALL from January 1, 2010 to May 31, 2016. The clinical data of 12 cases of patients with DVT were retrospectively analyzed, 183 cases diagnosed before January 1, 2015 were received chemotherapy with the scheme of SCMC-05. The other cases were treated by the scheme of CCCG. All the patients received central venous catheter., Results: The DVT happened in 12 cases including 10 cases of limb DVT and 2 cases of intacranial venous sinus thrombosis. The DVT mostly occured in intermediate risk ALL patients, the infection and coagulopathy existed in most patients. They were treated with low molecular heparin(LWHP), among them 5 cases were given extubation; the thrombus disappeared in 6 cases after 1 week; the intracranial venous sinus thrombosis in 1 case did not obviously improved after 6 months of treatment. The ALL children with DVT were treated with LWHP when using L-ASP, as a result no thrombuses happened., Conclusion: Centralvenous catheter and chemotherapeutic drugs were the major cause of DVT. Abnormal coagulation, infection, and risk stratification are another risk factors for thrombosis. ALL children thrombosis are benefited from LWHP prevention when using L-ASP again.

Obejective: To investigate the expression level of suppressor of cytokine signaling SOCS3 mRNA in children's acute lymphoblastic leukemia(ALL); to analyze the relationship between the expresion level of SOCS3 mRNA and disease status and risks of ALL, and to explore the application of SOCS3 mRNA in evaluation of ALL disease status, risk and target therapy., Methods: The expression levels of SOCS3 mRNA in bone marrow mononuclear cells from 45 cases of newly diagnosed ALL at initial diganosis and induction remission and 13 normal children as controls were detected by SYBR Green fluorescence quantitative PCR; the correlation of SOCS3 mRNA expression level with risk and clinical characteristics of ALL was analyzed by means of statistical method; the immunofluorescence histochemistry method and laser confocal microscopy were used to detect the sites and expression level of SOCS3 mRNA in bone marrow samples of ALL patients., Results: The SOCS3 mRNA expression level at initial diagnosis of 45 ALL patients was significantly lower than that of normal controls (P<0.05), and that in induction remission of 45 patients was not significant different from normal controls (P<0.05); the SOCS3 mRNA expression level at initial diagnosis of patients with standasd and high risk was higher than that of patients with low risk (P<0.05). The 45 patients were divided into high expression and low expression groups according to SOCS3 mRNA expression level at initial diagnosis by median method, comparison of clinical characteristics in 2 groups was found that the SOCS3 mRNA high expression group had even more higher leukocyte count in peripheral blood, even more higher LDH level and much more poor prognostic genes; in addition, the high expression group showed more higher risk in comparison between 2 group., Conclusion: The SOCS3 mRNA expression is down-regulated at initial diagnosis, while recovered to normal level after induction remission of disease, thus the SOCS3 can be used as an indicator for evaluation of disease status. The high expression of SOCS3 mRNA up-regulates the disease risk, therefore the SOCS3 mRNA can be used as a factor for evaluation of ALL risk. The treatment of ALL via regulation of SOCS3 mRNA expression level maybe can become a new way. The regulation of SOCS3 mRNA expression level maybe can become a new way for treatment of ALL.

Objectives: To explore the relationship between normalization of tumor microvessels and CA9 for rh-Endostatin to inhibit Lewis lung cancer (LLC) and the expression level of CA9 in LLC., Methods: Lewis cells of logarithmic growth phase were collected and made into 1×10 6 mL -1 cell suspensions were prepared. The transplanted tumor model of LLC was established on C57/BL6 mice by injected 0.2 mL cell suspensions/mice into 40 C57/BL6 mice. 40 LLC mice were randomly divided into control group and rh-ES group (20 mice per group). Control group experienced treatment of intraperitoneal injection (ip) for 0.2 mL NS/d, while rh-ES group was treated for 5 mg rh-ES/(kg·d) from the first to the ninth day. The samples of 5 mice were obtained from day 2, day 4, day 6 and day 9 after treatment in control group or rh-ES group, respectively. CA9 was tested by IHC in LLC and paracancerous tissues and estimated by RT-PCR and ELISA in the each time point of both rh-ES group and control group,respectively., Results: The transplanted tumor model of LLC on C57/BL6 mice was established successfully. The expression of CA9 decreased on day 4 and day 6 in rh-ES group estimated by RT-PCR and ELISA, which indicated some great significance when compared with day 2, day 9 in rh-ES group and day 4, day 6 in control group ( P <0.05), and the expression of CA9 in day 2, day 4, day 6, day 9 tested by IHC was higher in LLC than in paracancerous tissues in control group ( P <0.05)., Conclusions: The expression of CA9 was higher in LLC. Rh-ES could have positive effect on LLC model of C57/BL6 mice, in day 4-6 (a brief normalized time course) decreased the expression of CA9 and reversed the tumor hypoxia.

Objective: To investigate the effects of proximal femoral locking plate (PFP) in treating osteoporotic intertrochanteric fractures and to analyze the failure cases., Methods: Totally 32 patients with osteoporotic intertrochanteric fractures of Evans I and II were treated with improved locking PFP, including 17 males and 15 females with an average age of 77.3 years old ranging from 70 to 86 years old. After operation, according to Harris hip scores, the hip function and therapeutic effects were evaluated., Results: The observed 32 patients' operative time was (60.5±15.7) min, intraoperative blood loss was (128.8±73.6) ml;perioperative blood transfusion was (224.0±72.7) ml. Hospitalization time was from 14 to 20 d with an average of 17.2 d. All patients were followed up from 6 to 18 months with an average of 14.1 months. The fracture healing time was from 3 to 6 months with an average of 3.1 months. One patient occurred internal fixation loosening and screw backward, 4 cases occurred urinary tract infection, 1 patient died of cardiovascular disease for 6 months postoperative, 2 patients died of a stroke for 1 year postoperative. No incision deep infection, peri internal fixation fractures, lower extremity deep venous thrombosis, internal fixation breakage, nonunion, severe coax vara and coax valgus occurred. The final Harris score was 89.74±6.84, the result was excellent in 10 cases, good in 16 cases, fair in 4 cases, and poor in 2 cases., Conclusions: Locking PFP can provide relative stable fixation to proximal end of osteoporotic femoral fractures, which is a good choice for the treatment of intertrochanteric fractures. It could provide stableness of fractures and bone union, even avoid screws loose or slide out., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose.