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To solve the water hazard problem of Jurassic coal seam roof sandstone in Yinxing No.1 Coal Mine of Ningxia Province, the direct relationship between the sedimentary environment of Jurassic coal seam roof sandstone and water abundance was analyzed and the water abundance of sandstone aquifer was evaluated on the basis of combing the geological and hydro-geological conditions of the mine. The mechanism and characteristics of water disaster in Jurassic coal seam roof sandstone were analyzed based on the study of the mechanical properties, mineral composition, water stability characteristics and water-conducting fracture development law of coal seam roof strata. In this paper, we propose the idea of comprehensively applying various measures of “exploration, drainage, discharge, interception and monitoring”, and focusing on the basic exploration work, using hydrophobic and depressurizing technology to control water damage. The water damage control effect of 110301 working face show that it was feasible to control the sandstone water damage on the roof of Jurassic coal seam through hydrophobic depressurization.

Objective To investigate whether alpha-bisabolol can effectively reverse ATP binding cassette subfamily B member 1 (ABCB1)-mediated multidrug resistance (MDR) in tumors and to explore the underlying mechanisms. Methods The network pharmacology was utilized to analyze the active components of Agrimonia Eupatoria, predict target genes, and perform GO and KEGG pathway analysis. Molecular docking and thermal stability assays were conducted to examine the interaction between alpha-bisabolol and ABCB1. The MTT assay was used to assess whether alpha-bisabolol can reverse ABCB1-mediated MDR. Mechanistic studies were performed using Western blot, immunofluorescence, and flow cytometry to explore how alpha-bisabolol reverses ABCB1-mediated MDR. Results Network pharmacology indicated that alpha-bisabolol, an active compound in Agrimonia Eupatoria, is closely related to tumorigenesis. Molecular docking and thermal stability assays showed that alpha-bisabolol forms a stable complex with ABCB1. Reversal assays demonstrated that alpha-bisabolol effectively reverses ABCB1-mediated MDR in tumor cells. Mechanistically, alpha-bisabolol did not affect the expression or localization of ABCB1 in tumor cells but inhibited its excretion function, thereby increasing the intracellular drug accumulation and effectively reversing the MDR in tumor cells. Conclusion Alpha-bisabolol effectively reverses ABCB1-mediated MDR in tumor cells, offering a potential new therapeutic strategy for tumor patients with chemotherapy resistance.