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Steady-state visual evoked potentials-based brain-computer interfaces (SSVEP-BCI) can map visual stimuli with dif-ferent frequencies to certain commands to control external devices.In order to explore the capacitability of AR-BCI on the number of stimuli and the influence of the number of multi-target stimuli on the classification accuracy of AR-BCI,4 layouts of stimuli with different numbers are designed in this study and displayed through HoloLens (AR) glasses.The comparative analysis shows that the classification accuracy of the 4 layouts gradually decreases with the increase of the number of stimuli,and the location of stimuli affects the accuracy of stimulus classification.In the similar experimental paradigm,the classification results of PC screen and AR display terminals are compared,and it is found that the increasing number of stimuli has a great impact on the classification performance of AR-BCI.Current study indicates that the amount of stimulus is a key factor affecting the construction of AR-BCI in AR environment.

Objective To investigate the protective effect and mechanism of placental-derived mesenchymal stem cells (P-MSCs) on lung injury in rats with severe acute pancreatitis. Methods A total of 36 healthy adult male SD rats were randomly divided into control group (n=12), SAP group (n=12) and P-MSCs group (n=12). SAP rat model was established by retrograde injection of 4% sodium taurocholate into biliopancreatic duct. P-MSCs (1×10⁶ cells/100 g) were delivered through the tail vein of the rats at 6 h after the induction of SAP. The rats were sacrificed 12 and 24 h after P-MSCs infusion. The ratio of wet to dry weight of lung tissue and the number of neutrophils, leukocytes and macrophages in bronchoalveolar lavage fluid (BALF) was calculated. The degree of pancreatic and lung injury was evaluated by HE staining. The concentration of amylase, lipase and inflammatory factors in serum was measured by ELISA. The MPO activity in pancreas and lung tissue was measured by MPO assay kit, and the mRNA expression levels of TNF-α, IL-1β in lung tissue were detected by RT-qPCR. The ratio of M1 and M2 in lung tissue was detected by immunofluorescence stain. Results Compared with SAP group, the ratio of wet to dry weight of lung tissue in P-MSCs group significantly decreased, the number of neutrophils, leukocytes and macrophages in BALF were significantly reduced (P

Objective To investigate the effect of abdominal paracentesis drainage (APD) on pancreatic cell apoptosis in severe acute pancreatitis. Methods Male adult SD rats were randomized into the sham operation (SO) group, SAP group, and APD group, with 18 rats in each group. In the SAP group, 5% sodium sulfonate was pumped into the retrograde pancreatic bile duct to prepare the SAP model. On this basis, a gastric tube was introduced into the right lower abdomen for drainage, namely the APD group. Blood from the abdominal aorta and pancreatic tissues were collected at 6, 12, and 24 h time points in each group. The changes of serum amylase, inflammatory factor, and endotoxin were detected by ELISA. The HE staining was used to evaluate the pancreatic tissue injury. The apoptosis of pancreatic tissue was detected by TUNEL. Western blot and immunohistochemistry were used to detect the expression of apoptosis-related proteins and PI3K/AKT signaling pathway. Results Pancreatic tissue necrosis and edema were significantly lower in the APD group than in the SAP group, and the pathological score was decreased (P

Objective To observe the inflammatory response and peritoneal macrophage polarization phenotype of hypertriglyceridemic acute pancreatitis (HTG-AP) mice, and to record HTG-AP disease progression. Methods Adult male C57/BL6 mice were randomly divided into the control group, HTG group, AP group, and HTG-AP group, with 8 mice in each group. Mice in HTG and HTG-AP groups were intraperitoneally injected with Poloxamer 407 every other day for 28 days to construct the hyperlipidemia animal model. All animals anesthetized and sampled after caerulein treatment in the AP group and the HTG-AP group 12h later. Blood was collected to detect serum lipid and cytokine levels. HE staining was performed on the pancreatic tissues and pathological scores were obtained. The abdominal cavity was lavage with PBS to collect peritoneal macrophages, which were labeled by F4/80, CD11c, and CD206 and then detected by flow cytometry. Results The HTG-AP model of mice was constructed. Compared with AP group, pancreatic tissue pathology score, serum pro-inflammatory cytokines tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6 levels, as well as the percentage of M1 polarization of peritoneal macrophages, were significantly increased (P

IgG4-related sclerosing cholangitis is a biliary tract manifestation of IgG4-related diseases, characterized by biliary strictures, neoplastic hyperplasia of bile duct and its surrounding tissues, an increased serum level of immunoglobulin G4 (IgG4). The pathogenesis has not been fully elucidated and histopathological features include infiltration of lympho-plasmcytes, storiform fibrosis and obliterative phlebitis. Response to corticosteroids is prompt and multi-organ involvement is common. As a result of mimicking primary sclerosing cholangitis and cholangiocarcinoma in image, misdiagnose and missed treatment are not rare. Incorporating literature involved, we will review the pathogenesis, clinical manifestations, diagnostic criteria, treatment and prognosis of IgG4-related sclerosing cholangitis. DOI: 10.11855/j.issn.0577-7402.2019.10.13

Acute pancreatitis (AP) is a systemic inflammatory disease with complex pathogenesis. Acinar cell injury is the earliest pathological change of AP. Elucidating its pathological mechanism is of great significance for the development of new therapeutic strategies for AP. Studies have shown that activation of PI3K/Akt signaling pathways plays an important role in the progression of acute pancreatitis. It can not only contribute to acinar cell necrosis by inhibiting apoptosis, but also regulate the autophagy, oxidative stress and inflammation of acinar cells. Here, we reviews the advances about the PI3K/Akt signaling pathway and its significance in modulation of acinar cells in acute pancreatitis. DOI: 10.11855/j.issn.0577-7402.2019.09.13

Objective To investigate the clinical features of multiple ligament injuries of the knee joint, and evaluate the clinical efficacy of one-stage repair and reconstruction under an arthroscope of multiple ligament injured knee joint. Methods The clinical data were analyzed retrospectively of 22 patients receiving treatment and follow-up for multiple ligament injuries of the knee joint from March 2015 to June 2017 in the Henan Orthopedic Hospital. 13 males and 9 females aged from 24 to 64 years; and left knee injury occurred in 7 cases, right knee in 5 cases, all injuries were close with knee subluxation or dislocation. Imaging, clinical and arthroscopical examination showed anterior and posterior cruciate ligament ruptures in all the patients. The time from injury to operation was from 4 to 16 days, all the patients were treated by single tunnel and single beam reconstruction of anterior and posterior cruciate ligament under an arthroscope, limited incision and reconstruction of PMC (posterior medial complex) and PLC (posterior lateral complex); and the concurrent injuries were treated at that same time. Postoperative X-Ray and MRI were performed to determine the healing of bone tunnel, internal fixation, remodeling of reconstructed tendon and tendon healing. The knee function was assessed on the basis of the range-of-motion of the knee, IKDC score and Lysholm Knee Function score. Results All the patients were followed up from 6 to 14 months with an average of (11.0±1.5) months. The torn inner and outer meniscus and fracture were all healed and the deep venous thrombus was dissolved. Last follow up found varus and valgus stress test was normal or close to normal in 22 patients by Lachman test, anterior and posterior drawer tests were negative, and the tibial shift distance forward or backward was smaller than 5mm. The patients had no subjective symptoms. X-Ray and MRI showed that the tibial femoral tunnel had healed, and the position of internal fixation had no change, and the ligament had been reconstructed and the tendon had healed. In 2 cases, one year later, sensory and motor recovery was observed after common peroneal nerve injury. Range of motion of the knee joint, and Lysholm and IKDC scores were significantly superior at the last following up to at the preoperation (P

Objective To investigate the surgical method and clinical effect of arthroscopic fixation of tibial avulsion fracture of the anterior cruciate ligament (ACL) with hollow screw via medial patellar approach. Methods From July 2014 to December 2017, 26 ACL patients, including 18 males and 8 females, with an average age of (26.3±3.3) years, ranging from 19 to 50 years, were treated by arthroscopic hollow screw fixation through medial patellar approach. According to Meyers-McKeever- Zaricznyj before operation, there were 10 cases of type Ⅱ fracture and 16 cases of type Ⅲ, all of which were fresh fractures. The preoperative and postoperative anterior drawer test, Lachman test, Lysholm knee function score and International Knee Documentation Committee (IKDC) score were compared. All the operations were performed by the same operation team. Results The follow-up period ranged from 6 to 14 months with an average of (11.2±1.5) months, and 2 patients were lost to follow-up. X-ray radiographs showed that all fractures healed 3 months after the surgery, and there was no case of screw loosening, fracture or re-fracture. At the last follow-up after surgery, the active range of the affected knee was extension 0°, flexion 130°-150° with an average of 138°. Both the anterior drawer test and Lachman test changed from positive to negative before surgery; Lysholm's knee function score improved from (46.50±3.31) points before operation to (89.13±2.52) points at the last follow-up, the difference was statistically significant (t=50.13, P

Exercise-induced fatigue is a comprehensive response to a variety of physiological and biochemical changes in the body, and can affect people's quality of life to different extents. If no timely recovery after occurrence of fatigue, accumulated gradually, it can lead to "burnout", a "overtraining syndrome", "chronic fatigue syndrome", etc., which will cause endocrine disturbance, immune suppression, even physical illness. Exercise-induced fatigue becomes an important factor endangering human health. In recent years, many experts and scholars at home and abroad are committed to the research of exercise-induced fatigue, and have put forward a variety of hypothesis to explain the cause of exercise-induced fatigue. They expect to find out the methods for preventing and eliminating exercise-induced fatigue. This article discusses mainly the pathogenesis, model building, elimination/ relief, etc. of exercise-induced fatigue to point out the research achievements of exercise-induced fatigue and its existing problems. DOI: 10.11855/j.issn.0577-7402.2016.11.14

Objective To observe the therapeutic effect of bone cement injectable pedicle screw system combined with intervertebral fusion for lumbar spondylolysis and osteoporosis. Methods The clinical data were analyzed retrospectively of 21 patients with lumbar spondylolysis and osteoporosis who received treatment of bone cement injectable pedicle screw system and intervertebral fusion from Aug. 2013 to Nov. 2015. The 21 patients (9 males and 12 females) aged from 60 to 80 years (mean 64 years old); 6 of them presented degenerative spondylolysis, 15 with isthmic spondylolisthesis; 2 cases had I degree slippage, 13 had Ⅱdegree slippage, 6 had Ⅲdegree slippage, and all the cases were unisegmental slippage including 9 cases in L4 and 12 cases in L5. Bone mineral density of lumbar vertebrae (L2-L5) was measured with dual-energy X-ray absorptiometry, and T values conforming to the diagnostic criteria of osteoporosis were less than or equal to -2.5; All patients were operated with whole lamina resection for decompression, bone cement injectable pedicle screws system implantation, propped open reduction and fixation intervertebral fusion. The clinical outcomes were determined by the radiographic evaluation including intervertebral height, height of intervertebral foramen, slip distance, slip rate and slip angle, and Oswestry disability index (ODI) on preoperative, 3 months after operation and the end of the time, and the interbody fusion were followed up. Results Cerebrospinal fluid leakage of incision was observed in two cases after operation, compression and dressing to incision, Trendelenburg position, dehydration and other treatments were taken, and the stitches of incisions were taken out on schedule. Slips in the 21 patients were reset to different extent, and lumbar physiological curvatures were recovered. The intervertebral height and height of intervertebral foramen were obviously higher 3 months after operation than that before operation (P0.05). Based on the ODI score, the excellent/fine rate was 90.6% and 92.5% for 3 months after operation and the last follow-up, respectively. During the period of following up, no loss of the correction degrees, no loosening of the screws, and 19 cases were noted bony fusion with the fusion success rate of 90.5%. Conclusion The treatment effect of bone cement injectable pedicle screw system and intervertebral fusion for lumbar spondylolysis and osteoporosis is satisfied. DOI: 10.11855/j.issn.0577-7402.2016.10.12

Objective To investigate the effects of percutaneous midband pulse current stimulation in hepatic region on anti-exercise fatigue ability and the free radicals and nissl bodies in cerebral cortex tissue of rats with exercise-induced fatigue. Methods Seventy-two 8-week old male Wistar rats were randomly divided into 4 groups (18 each): control group (CG), fatigue group (FG), stimulation before fatigue group (SBF) and stimulation after fatigue group (SAF). Animals in FG, SBF and SAF group were used to reproduce the swimming-exhaustion models. Midband current stimulation (1024Hz, 10mA, current cycle 1sec) for 20 minutes was given to the rats of group SBF before swimming, and to those in group SAF after exhaustion. At the weekend of the 1st, 3rd and 5th week after modeling, the exhaustive swimming time of rats in all but CG group was observed. Cerebral cortex tissue was harvested for the estimation of the level of lipid peroxidation, including SOD, MDA, GSH-Px and SOD/MDA, and the histopathological changes in nissl bodies in neurons were observed. Results At the 1st weekend after modeling, no significant difference was found in all the indexes among the 4 groups, while at the 3rd weekend, the exhaustive time was obviously longer in SAF group than in FG group, and also in SAF group than in FG and SBF group at the 5th weekend (P

Objective To explore the effects of percutaneous impulsive current stimulation in hepatic region on the activity of hepatic mitochondrial Na+-K+-ATPase and Ca2+-Mg2+-ATPase in exercise-induced fatigued rats, in order to investigate the effect of exercise-induced fatigue. Methods Seventy-two 8-week old male Wistar rats were randomly divided into 4 groups (18 each): control group (group A), fatigue group (group B), stimulation before fatigue group (group C) and stimulation after fatigue group (group D). Exhaustion of animals in B, C and D groups were reproduced by prolonged swimming. Current stimulation (1024Hz, 10mA, current cycle 1sec) for 20 minutes was given to the rats of group C before swimming, and to those in group D after exhaustion. At the weekend of 1st, 3rd and 5th week after modeling, the rats were sacrificed in batches from each group (6 each). The activities of hepatic mitochondrial Na+-K+-ATPase and Ca2+-Mg2+-ATPase were determined by spectrophotometry, and Bradfood protein quantification was employed to quantitate the protein in rats' hepatic mitochondria. Results No significant difference was found in swimming-exhaustion time among 3 groups at the first weekend (P>0.05), while the swimming-exhaustion time was significantly prolonged at the 3rd and 5th weekends in group D than in group B and C (P0.05), while the enzyme activities were obviously lower at the 3rd and 5th weekend in group B than that in groups A, C and D (P

Objective To explore the influence of percutaneous stimulation of the hepatic region with mid-frequency pulsed current on the serum activity of glutathione peroxidase (GSH-PX), superoxide dismutase (SOD) and total antioxidant capacity and content of malondialdehyde (MDA) in exercise-induced fatigued soldiers. Methods Sixty healthy male recruits without training history were randomly divided into control group and stimulation group (n=30). Subjects in both groups received intensive training for 5 weeks (trained from Monday to Saturday, and rest on Sunday) to establish an exercise-induced fatigue model. The recruits in stimulation group received rehabilitation therapy of percutaneous stimulation of the hepatic region with mid-frequency pulse current (frequency was 1024Hz, dynamic cycle 1s, stimulation time 20min, output intensity ≤80mA) after the training immediately. In every Sunday morning of the 1st, 3rd and 5th week, venous blood samples were obtained from recruits of both groups for determination of the serum activity of GSH-PX, SOD and T-AOC and content of MDA. Results In both groups, the serum activity of GSH-PX and T-AOC on 5th weekend was lower than that of 1st and 3rd weekends, and the serum activity of GSH-PX and T-AOC on 3rd weekend was lower than that of 1st weekend (P0.05); the serum MDA content on 5th weekend was higher than that of 3rd and 1st weekends, and the content on 3rd weekend was higher than that of 1st weekend (P

Objective To explore diagnostic method and surgical outcome of lateral patellofemoral crush syndrome caused by military training. Methods Fifteen patients with lateral patellofemoral crush syndrome caused by military training from May 2006 to May 2008 were enrolled in this study, including 12 men (14 knees) and 3 women (3 knees), aged from 22 to 43 years old with an average of 27.3 years. Randomly selected 18 healthy volunteers with similar age and gender but no knee pain symptom were selected to serve as a control group. Anteroposterior and lateral X-ray photographs of knee joint and axial photographs of the patella were taken in both groups for observing the bone architecture of the knee joint and measuring the femoral trochlear angle, patellofemoral congruence angle and patellofemoral index, and the data were compared. The 17 knee joints in patient group were treated with lateral patellar retinaculum release. Then the preoperative and postoperative pain severity was evaluated with visual analogue scale (VAS). Results Patellofemoral congruence angle was 7.67°± 5.81° and patellofemoral index was 2.49±1.40 in patient group, while they were －2.2°±－2.71° and 1.25±0.15 in control group. The difference between two groups showed statistical significance (P < 0.05). The change in bone architecture was obvious in patient group. The preoperative pain score was 7.06±0.85, and postoperative pain score was 3.87±0.24 after 6 months and 3.01+0.17 after 1 year in patient group. The difference between preoperative pain score and postoperative pain score showed statistical significance (P < 0.05). Conclusions Diagnosis of patellofemoral crush syndrome caused by military training requires a combination of case history, typical symptoms, objective sign and X-ray examination. Among these, X-ray examination is the fundamental auxiliary diagnostic tool for lateral patellofemoral crush syndrome, and the patellofemoral index is convenient for diagnosis.

Objective To observe the effect of cyclic training on histomorphology of the terminal structure of rabbit Achilles tendon, and explore its preventive effect on training-based enthesiopathy. Methods Seventy-two Japanese white rabbits were randomly assigned to four groups: control group, jumping group, running group and cyclic training group, 18 for each. Three rabbits of each group were sacrificed at the 2nd, 3rd, 4th, 6th, 8th and 10th week. The terminal insertion tissues of bilateral Achilles tendons were harvested from these rabbits for observing the pathomorphological changes under light microscope, and pathological scoring and statistical analysis were carried out. Results Light microscopy showed that the tendon fibers and fibrocartilage in the Achilles tendon insertion region were severely damaged in the jumping group, and though the tendon fibers were damaged severely, the injury to the fibrocartilage was comparatively less serious in the running group. The injuries to the tendon fibers and fibrocartilage were milder in the cyclic training group than in the jumping group and running group. In the 2nd, 3rd, 4th, 6th and 8th week, the histopathology score of insertion of Achilles tendon was 1.17±0.12, 2.19±0.15, 3.23±0.20, 4.66±0.16, 4.71±0.18, 4.63±0.13 respectively in the jumping group, and 1.16±0.13, 1.15±0.14, 2.18±0.12, 2.99±0.15, 3.98±0.16, 4.01±0.12 respectively in the running group. Increase in pathological score appeared earlier in the jumping group than in the running group, and a significant increase began at the 3rd week. The difference in pathological score between the two groups originated mainly from the changes in the tidemark. In the 2nd, 3rd, 4th, 6th, 8th and 10th week, the pathological score of Achilles tendon insertion was 1.13±0.14, 1.16±0.17, 1.15±0.13, 2.18±0.13, 2.17±0.12, 2.92±0.11 respectively in the cyclic training group, and they showed no significant changes as compared with control group at the early stage, but there was an increase at the late stage of the training (6 weeks later), however the score values were lower than those in the jumping group and running group. These changes in score were originated mainly from pathological changes in the paratenon and tidemark. Conclusions Jumping training could lead to severe injury to the fiber tissue and fibrocartilage, while running training might result in severe injury to Achilles tendon insertion structure and lighter injury to the fibrocartilage part. Cyclic training model provides suffcient time for repair and reconstruction of Achilles tendon insertion structure, and decrease the severity of the injury to Achilles tendon insertion region. Therefore, use of cyclic training could not only effectively prevent training-based enthesiopathy, but also accelerate the remodeling of the tissue in the insertion region, thus strengthen the repair ability of Achilles tendon against injury.

Objective To investigate the anti-fatigue effect of percutaneous stimulation of the hepatic region with the mid-frequency pulse current in different diadynamic cycles in exercise-induced fatigued soldiers. Methods One hundred twenty healthy PLA recruits who did not have physical exercise were randomly divided into four groups with thirty ones in each: control, stimulation group A, stimulation group B, and stimulation group C. All the subjects of four groups were ordered intensive training (exercise from Monday to Saturday, with rest on Sunday) for five weeks to establish the exercise-induced fatigue model. Each day after the exercise, the recruits of stimulation groups A, B, and C were treated immediately with mid-frequency (1204Hz, current intensity ≤80mA) stimulation to the hepatic region with diadynamic cycles of 0.5, 1, and 2 seconds, respectively. No pulse current stimulation was given in the control group. Venous blood was collected before breakfast on Sundays to measure the fasting plasma glucose (FPG) and blood lactate (LAC) contents, and liver function was determined by determination of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH). The 3000-m running performance of the recruits in each group was recorded on the same day. Results There was no significant difference between the four groups in terms of the FPG level at the end of the first week (P>0.05). At the end of the third and fifth weeks, the FPG level was significantly higher in the three stimulation groups than in the control group (PPP>0.05). At the end of the first, third, and fifth weeks, the ALT, AST, LDH, and LAC levels were significantly lower in every stimulation group than in the control group (PPPPP>0.05). At the end of the first week, there was no significant difference in 3000-m running performance (P>0.05) between the 4 groups. At the end of the third and fifth weeks, the 3000-m running performance was significantly better in every stimulation group than in the control group (PPPP>0.05). Conclusions Percutaneous stimulation of the hepatic region with mid-frequency pulse current in different diadynamic cycles can reduce liver injury due to exercise-induced fatigue, promote the generation of energy source in vivo, accelerate the elimination of lactate, improve the exercise endurance and exert a role in relieving exercise-induced fatigue, and the efficacy of the mid-frequency pulse current with 1204 Hz and diadynamic cycles of 1 second is the best.

To conduct a random sampling survey in occurrence and distribution of military training-related injuries in the troops as the monitoring sites of the injury in the years of 2009 and 2010 for analyzing the epidemiological features and trend of the injury. Methods Ten troops as the monitoring site were randomly selected for the survey, and soldiers who participated in military training in the years 2009 and 2010 served as the objects of the survey. Data concerning military training-related injuries were collected and analyzed according to the "Diagnostic Criteria and Treatment Principle on Military Training-Related Injury". Results Among the 63132 soldiers who participated in various military trainings, 10775 suffered from military training-related injuries in 2009 and 2010. Among which, 4076 out of the 27230 soldiers who participated in military training in 2009 suffered from training-related injury, with an incidence of 15%; while 6699 out of the 35902 soldiers who participated in military training in 2010 suffered the injury, with an incidence of 18.7%. There was a significantly higher incidence of military training-related injuries in 2010 (P＜0.01). The composition ratios of osteoarticular, soft tissue, and organ injuries were 33.5%, 48.4%, and 18.1% in 2009, and 19.7%, 73.3%, and 7% in 2010, respectively. The composition ratio of organ and osteoarticular injury decreased, while that of soft tissue injuries increased significantly. Two peaks of training injuries were seen both in 2009 and 2010. Conclusions More attention should be paid to the prevention of injuries related to military training. The key to reducing the incidence of military training-related injuries is to enhance the dissemination of information about military training-related injuries and strictly carry out the "Rule for Health Protection in Military Training".

Objective To investigate the effects of the different training patterns on the biomechanical performance of the terminal region of the Achilles tendon for providing a scientific basis for improving the ability of anti-stress and preventing injury in military physical training. Methods Fifty Japanese white rabbits were randomly assigned to four groups: control (n=5), running (running training on the running table, n=15), jump (jump training by electric shock, n=15), and cycle training (run and jump cycle, n=15). The animals in the control group were sacrificed at the start of the experiment. For the other training groups, three rabbits were sacrificed randomly in the second, third, fourth, sixth, and eighth week, respectively. The terminal regions of the Achilles tendons in both sides were identified to determine the biomechanics. Results At the sixth week, the limit stress and the elastic modulus reached the highest in the running group, and then reduced with the increase in training time. Meanwhile, in the jump group, the limit stress and elastic modulus reached the peak at the fourth week, and then decreased gradually. In the eighth week, no significant difference was observed when the groups were compared with the control group. In the cycle group, the limit stress and elastic modulus increased gradually with the increase in training time, reaching the peak value in the eighth week, and no decline was recorded during the training period. Conclusions Running training can slowly improve the biomechanical performance of the tissues in the terminal region of the Achilles tendon, whereas jump training can rapidly increase it, although long-term excessive training will reduce the anti-injury ability. Meanwhile, cycle training can improve the biomechanical performance of the tissues in the terminal region of the Achilles tendon, enabling it to withstand more stress and thus its anti-injury ability is enhanced.

Objective: To investigate PTEN gene expression and the Akt phosphorylation of protein expression in the LPS-induced lung fibroblast, to initially reveal the relation between PTEN gene and the Akt phosphorylated proteins to LPS-induced lung fibroblast proliferation mechanism. Methods: BrdU experiments was performed to evaluate the LPS-induced lung fibroblast proliferation, RT-PCR and Western Blot analysis were used to analyze the PTEN gene expression and Western blot was performed to analyze Akt phosphorylated protein expression. Results: PTEN mRNA level of the experimental group were significantly lower than the control group (PP>0.05) . PTEN protein expression levels of the experimental group were significantly lower than the control group (Pp>0.05). Phosphorylation Akt protein level (relative to total Akt protein) was significantly higer than the control group (PP>0.05) .Conclusion: PTEN gene and phosphorylation Akt protein involve in LPS-induced lung fibroblast proliferation signal transduction pathway.