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Objective: To investigate the contamination of antibiotic-resistant bacteria in air of different departments in hospital., Methods: From 2018.07 to 2021.06, 191 samples of the air-conditioning filter dust in three hospitals were collected. Antibiotic-resistant bacteria were isolated from the accumulated dust. The drug sensitivity test was conducted for Staphylococcus aureus, Acinetobacter baumannii and Enterobacteriaceae., Results: A total of 119 samples were detected antibiotic-resistant bacteria from 191 samples, and the detection rate was 62.30%. The detection rate of different departments from high to low was surgical ward(68.29%) >intensive care unit(ICU)(59.62%) >medical ward(57.92%). A total of 362 strains of antimicribial-resistant organisms were isolated, mainly were Acinetobacter(28.73%), Pseudomonas(22.10%), Bacillus(22.10%), Staphylococcus(9.12%), etc. Among them, 72 strains of target organisms were detected, and the detection rate was 19.89%(72/362), the detection rate of different target bacteria from high to low was Acinetobacter baumannii(12.71%)>Enterobacteriaceae(4.72%)>Staphylococcus aureus(2.76%)(P<0.05). The drug sensitivity test showed that 41 strains of antimicribial-resistant organisms were detected, and the detection rate was 56.94%(41/72), including carbapenem-resistant Acinetobacter baumannii(CR-ABA), methicillin-resistant Staphylococcus aureus(MRSA), carbapenem-resistant Enterobacteriaceae(CRE), etc.24 strains of multidrug-resistant organisms(MDROs) were detected and the detection rate was 58.54%(24/41). The detection rate of different departments from high to low was ICU(80.00%)>medical ward(60.00%)>surgical ward(46.15%)., Conclusion: There was contaminated by Acinetobacter baumannii, Staphylococcus aureus, Enterobacteriaceae in the air of hospitals, some of them were MDROs, mainly were detected in neurological ward, respiratory medical ward, hyroid and breast surgery ward, neurosurgery ward, cardiothoracic surgery ward, gallideulous surgical ICU and general ICU.

Objective: To conduct a continuous monitoring of the physical, chemical microbial indicators and overall biological toxicity for water quality in waterlogged urban areas., Methods: Monitoring sites were chosen in the waterlogging areas in the NanhuYayuan and Banqiao communities of Hongshan District, and the Xinhu village of Xinzhou District, Wuhan City. The South Lake, Yezhi Lake and Shahe River were selected as the corresponding surface water monitoring sites. Samples were collected in an extended period of time for the examination of physical, chemical microbial indicators and the determination of biological toxicity. Statistical analysis was performed using SPSS 11. 0 software package., Results: The water samples from waterlogged areas generally exhibited poor aesthetic aspects of drinking waters, and these indexes gradually deteriorated with prolonged waterlogging. Among the general chemical indexes, p H value was at an initialhigh level and decreased with time, permanganate index( oxygen consumed) remained at a high level( 3. 83-8. 16 mg/L), nitrite-nitrogen level gradually increased and stabilized for 2-3 days and then gradually decreased, chloride of the waterlogged samples and the corresponding surface water samples were correlated. Toxicological indicators( arsenic, lead, mercury) were not examined. For microbioligical indicators, fecal coliforms was detected at a rate of 47%, and negative result were revealed for pathogenic pathogenic microorganisms( Escherichia coli O157, Vibrio cholerae, Shigella, Salmonella, Staphylococcus aureus) and Rotavirus and Novovirus. The inhibition rate of samples on luminous intensity of Vibrio cholerae was less than 30%., Conclusion: Although the aesthetic aspects of drinking water were poor and organic and fecal contaminants were present, the risk of health hazards from skin or respiratory exposure was small for residents. After the floodwater subsided, routine preventive disinfection will be sufficient to ensure the safety for waterlogged urban areas. The result of the luminescent bacteria test were in good agreement with the toxicological indicators.