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Objective To explore the efficacy and safety of microporous polysaccharide hemostatic material in endoscopic treatment of peptic ulcer bleeding. Methods Twenty-five cases of peptic ulcer with bleeding diagnosed under endoscopy in our hospital were collected. The endoscopic hemostasis therapy was conventional hemostasis （mechanical hemostasis,electrocoagulation） combined with microporous polysaccharide hemostatic material for electro-positive pressure spraying hemostasis therapy （observation group）. Twenty-five cases of peptic ulcer patients with bleeding treated by conventional hemostatic methods （control group） were compared in the same period. The success rate of intraoperative hemostasis within 5 minutes, the incidence of postoperative delayed bleeding, and the success rate of hemostatic treatment were compared between the two groups. Results In the observation group, the success rate of hemostasis within 5 minutes was 100%, the incidence of delayed hemorrhage was 0, and the success rate of hemostasis treatment was 100%. Whereas the success rate of hemostasis within 5 minutes, the incidence of delayed hemorrhage, and the success rate of hemostasis in the control group were 92%, 24%, and 72%, respectively. Compared with the hemostatic effect of the two groups, the success rate of hemostatic treatment in the observation group was significantly higher than that in the control group （P < 0.05）, otherwise the incidence of delayed bleeding was lower （P < 0.05）. Conclusions The combined use of the microporous polysaccharide hemostatic material can improve the success rate of endoscopic hemostatic therapy for peptic ulcer bleeding and reduce the incidence of delayed bleeding, which is an effective and safe method worthy of promotion.

In order to investigate the chronic toxicity of strobilurins to the early life stage of aquatic organisms, the sublethal effect of kresoxim-methyl on the early development of zebrafish after long-term exposure was determined. After continuous exposure of zebrafish embryos to different concentrations of kresoxim-methyl (0.000750 ~0.180 mg·L-1) for 34 d, the median lethal concentrations (LC50) of kresoxim-methyl for 144 h and 34 d of zebrafish early life stage was 0.141 mg·L-1 and 0.0106 mg·L-1, respectively. It was found that 0.00460 mg·L-1, 0.0115 mg ·L-1 and 0.0288 mg·L-1 kresoxim-methyl significantly decreased the body weight of larvae after 34 d exposure, 0.0115 mg·L-1 and 0.0288 mg·L-1 kresoxim-methyl decreased the body length of larvae, and 0.180 mg·L-1 kresoxim-methyl increased the body length and body weight of larvae. The 34 d-NOEC and 34 d-LOEC of kresoxim-methyl was 0.00460 mg·L-1 and 0.0115 mg·L-1 for body length, and was 0.00185 mg·L-1 and 0.00460 mg·L-1 for body weight during zebrafish early life development, respectively. During the 34 d exposure, the activities and levels of factors related to mitochondrial energy metabolism and oxidative stress in zebrafish embryos/larvae were measured at 1, 3, 7, 14, 21 and 34 d. Results showed that long-term exposure of kresoxim-methyl could increase the catalase (CAT) activity, the peroxide (POD) activity increased then decreased and then increased, the superoxide dismutase (SOD) activity and malondialdehyde (MDA) content decreased first then increased during zebrafish early life development. It was found that the calcium ion (Ca2+) level and the activity of calcium ion adenosine triphosphatase (Ca2+-ATPase) was inhibited during the 34 d exposure of kresoxim-methyl, and the adenosine triphosphate (ATP) level was increased first then decreased. The results suggested long-term exposure to kresoxim-methyl caused negative effects on the growth indexes and antioxidant system during zebrafish early life development, kresoxim-methyl might interfere with mitochondrial energy metabolism by regulating the ATP and Ca2+ levels, and Ca2+-ATPase activity, then induced oxidative stress during the early life stage of zebrafish. [ABSTRACT FROM AUTHOR]

Objective: To observe the incidence of intensive care unit-acquired weakness (ICU-AW) of mechanically ventilated patients, and to identify the relevant risk factors., Methods: A prospective cohort study was conducted. The patients admitted to intensive care unit (ICU) of Fuxing Hospital, Capital Medical University, aged 18 years old or older, with the duration of mechanical ventilation ≥ 24 hours and expected to stay in ICU for ≥ 7 days from May 2015 to January 2016 were enrolled. From the 7th day after ICU admission, the patients were evaluated for consciousness every day. If the patient was awake and could cooperate with muscle strength measurement, the day was recorded as T1, and the patient's muscle strength was measured using the Medical Research Council scale (MRC) and recorded, then all patients were divided into two groups according to MRC score, ICU-AW group (MRC score < 48) and non-ICU-AW group (MRC score ≥ 48). The death, transfer or the 28th day of ICU admission were regarded as the end of observation. The data from the first day of ICU admission to T1 (before T1), including metabolic factors (the lowest value of blood sodium, blood potassium, blood calcium, albumin, and the highest value of blood glucose), mechanical ventilation factors (mode and duration of mechanical ventilation), organ dysfunction factors [occurrence and duration of sepsis, multiple organ dysfunction syndrome (MODS)], and drug factors (whether the patients used aminoglycoside, sedative, muscle relaxant or glucocorticoids, etc., the time of these drugs usage and the cumulative dose) of the patients were observed, recorded and analyzed, as well as the data from T1 to the end of the observation period, including the duration of mechanical ventilation, incidence of ventilator associated pneumonia (VAP), 28-day mortality, the length of ICU stay, and the cost of ICU and hospitalization. The relevant factors with statistical significance in univariate analysis were enrolled in multivariate analysis, and Logistic regression equation was established to screen the independent risk factors that might lead to ICU-AW., Results: 486 patients with mechanical ventilation were enrolled in this study, and 37 patients were enrolled according to the inclusion and exclusion criteria, including 15 patients with ICU-AW (with ICU-AW incidence of 40.5%) and 22 patients without ICU-AW. In the univariate analysis, ICU-AW group patients showed statistical differences in following factors as compared with the non-ICU-AW group: age, and the duration of invasive ventilation and the total duration of mechanical ventilation, braking time, sepsis, MODS and duration of them, the usage days and dosage of sedative and glucocorticoid before T1. The total duration of mechanical ventilation from T1 to the end of the observation period, total duration of mechanical ventilation during the observation period, and length of ICU stay of the ICU-AW group were significantly longer than those of the non-ICU-AW group [hours: 190 (110, 274) vs. 4 (0, 57), hours: 337 (237, 477) vs. 78 (43, 170), days: 20±7 vs. 14±7, all P < 0.05], the incidence of VAP, 28-day mortality and total hospitalization expenses were significantly higher than those of the non-ICU-AW group [26.7% (4/15) vs. 0% (0/22), 53.3% (8/15) vs. 9.1% (2/22), 10 thousands Yuan: 18.7±7.7 vs. 12.1±10.8, all P < 0.05]. Multivariate Logistic regression analysis showed that the total duration of mechanical ventilation between the patient awake and ICU admission [odds ratio (OR) = 1.03, 95% confidence interval (95%CI) was 1.01-1.05, P = 0.011] and the duration of MODS (OR = 1.79, 95%CI was 1.17-2.72, P = 0.007) were the independent risk factors for ICU-AW in mechanically ventilated patients., Conclusions: ICU-AW is quite common in patients with mechanical ventilation and has a higher incidence. The risk factors associated with ICU-AW were the total duration of mechanical ventilation and duration of MODS.