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Objective To explore the application value of automatic nucleic acid extractor combined with vacuum concentrator in forensic DNA extraction. Methods Gradient samples of human peripheral venous blood were collected at 40, 80, 120, 160, 200, 240, 280 and 320 fold dilution. The samples of each gradient were treated with no inhibitor, black oil, rust, fruit acid, tin foil and indigo, respectively. The automatic nucleic acid extractor was used for DNA purification and extraction of the above samples. The extracted DNA eluent （6 μL） was taken for amplification directly, and the rest was concentrated by vacuum concentrator. DNA was amplified and examined using the Investigator 26plex QS kit before and after concentration. Results Only gradient samples treated with fruit acid obtained complete STR typing results at 40 fold dilution. The other 5 methods obtained complete STR typing results at 40-160 fold dilution. The results of STR typing after DNA concentration showed that the average peak height and detection rates of gene loci both increased to a certain extent, but the effect was not obvious. Conclusion The automatic nucleic acid extractor has an efficient inhibitor removal ability and high extracting efficiency of DNA. The vacuum concentrator can concentrate DNA samples to a certain extent. Combining the automatic nucleic acid extractor with the vacuum concentrator can improve the examination success rate of forensic materials.

After solution treatment at 1300℃ and cold rolling deformation at different reductions, 2205 duplex stainless steel was homogenized at different temperatures for different time intervals. To study the precipitation behavior of σ phase in the steel, the microstructure of specimens was observed by optical microscopy and transmission electron microscopy, and the volume fraction of σ phase in the microstructure was calculated by Image Tool software. The results show that when the steel is homogenized at 950℃, the precipitation time of σ phase is shortened from 30min to 3 rain with the increase of cold rolling reduction from 50% to 85%. As to specimens with a cold rolling reduction of 85%, the volume fraction of σ phase increases from 1.2% to 11.8% when the homogenizing time is prolonged from 3 min to 30 min at 950℃ However, after homogenization for 5 min, the volume fraction of σ phase decreases from 8.9% to 3.6% with the rise of temperature from 875 to 950℃; moreover, when the homogenizing temperature is 975℃, No phase exists in the specimen.

Acyl -CoA: diacylglycerol acyltransferase (DGAT) is the only key enzyme evolved in the Kennedy pathway and catalyzes the last step. This paper summrized the features of DGAT1 (subtype 1 of DGAT family) in eukaryote, including construction, substrate specificity, and spatio - temporal expressions. It especially reviewed DGAT1 function on plant growth and development. In discussion, some suggestions were made for further study of DGAT1 in plant. [ABSTRACT FROM AUTHOR]

After the solution treatment and cold rolling deformation of 00Cr22Ni5Mo3N duplex stainless steel, its super plastic deformation test is made at 850 ~ 1 000 °C for 5 minutes. The effect of deformation temperature on the superplasticity of 00Cr22Ni5Mo3N is studied. Its microstructure before deformation is observed, and the influence of sigma phase and microstructure changes on the superplasticity is researched. The reason why the deformation temperature affects the superplasticity of 00Cr22Ni5Mo3N is revealed. The results show that, when the deformation temperature changes, the microstructure and quantity of σ phase changes, and so does the superplasticity. After solution treatment at 1 300 °C, 85% of cold rolling deformation, and holding at 850 - 1 000 °C for 5 rain, its microstructure ~s recrystallized, the uniform equiaxed grains become more and more with the increase in deformation temperature, and the number of sigma phase gets smaller. Meanwhile, the elongation of00Cr22Ni5Mo3N duplex stainless steel increases from 480% to 1290%, and the peak stress decreases from 125 MPa to 32 MPa. [ABSTRACT FROM AUTHOR]

In this paper, the author presented a strategy for classifying local multi-channels MS waveform, triggering by a single event. There were three steps to achieve the goal. Firstly, based on STA/LTA method, the first arrival and terminated time had been picked up, using the MS signal preprocessed; secondly, the author extracted the waveform features, time-frequency (L,f), amplitude (A), statistics of amplitude distribution (AD) and threshold algorithm (TS), and correlation coefficient (R); thirdly, before establishing an effective judgment mechanism, this method employed a hierarchical recognition framework with 3 layers, which integrated preliminary judgment, combined recognition and optimization judgment. This method was validated through analyzing a coal mine visual event in Shandong Province, and the result shows that it is successfully used to classify electromagnetic interference wave, background noise and MS events. The result can basically meet the requirements of classification accuracy. [ABSTRACT FROM AUTHOR]

Objective: To investigate the molecular mechanisms underlying the treatment of inflammatory bowel disease (IBD) by Pulsatilla decoction., Methods: 84 BALB/c mice were randomly divided into ethanol control group, model group, SASP group, Pulsatilla decoction group (further divided into low, middle and high dose group) and zVAD group (n = 12). Intragastric and celiac drug administration were used in each group respectively. The expression of NLRP3, ASC, Caspase-1, IL-18 and IL-1beta in the colons were detected by fluorescence quantitative RT-PCR, Elisa and immunohistochemistry after treatment respectively., Results: Compared with the model group, SASP group, middle and high dose group could treat IBD effectively (P < 0.01), while this effect was restrained in zVAD group (P < 0.05). In the meantime, middle and high dose group could effectively raise the expression of NLRP3, ASC, Caspase-1, IL-18 and IL-1beta in the colons (P < 0.05), while this effect was also inhibited in zVAD group (P < 0.05)., Conclusion: Pulsatilla decoction is likely to exert their therapeutic effect by activating NLRP3 inflammasome which further promote the formation of the corresponding inflammation factors such as 1L-18 and IL-1beta.

Objective: To investigate the clinicopathological characteristics, histological diagnosis, immunohistochemistry and prognosis of cervical glassy cell carcinoma (GCC)., Methods: The clinical characteristics, cytology, histology and immunohistochemistry were analyzed in 5 cases of GCC., Results: The average age of the five patients was 34.4 years (31 - 41 years). Abnormal vaginal bleeding and/or watery discharge were clinical presentations. One case was complicated with pregnancy and another one had a seven-year history of using contraceptives. All patients had an obvious mass in the cervix. Characteristic morphological features of GCC were present in 2 cases. Morphologically, the tumors consisted of clusters of tumor cells with distinct cell bounders, a large amount of eosinophilic granules in the cytoplasm imparting ground glass appearance, and thin nuclear membrane and prominent nucleoli. Nuclear enlargement and multinucleation were frequently noted. Mitosis and apoptosis were common. Numerous eosinophils and plasma cells were present in the stroma. Immunohistochemically, GCC expressed markers for both squamous cell carcinoma (p63 and CK34βE12) and adenocarcinoma (CAM5.2, MUC1, MUC2 and CEA). Ki-67 proliferation index was high (≥ 70%). All the five patients were treated with radical hysterectomy, followed by radiation and chemotherapy. The tumor-free survival time ranged from 25 days to 33 months., Conclusions: GCC is a distinct variant of adenosquamous carcinoma of the cervix with high proliferation index and expression of markers of both squamous cell carcinoma and adenocarcinoma. The tumor has characteristic cytological and histological features.

Objective: To investigate the roles of surviving and caspase-3 in the development of oral cancer., Methods: Archival tissue sections of 17 oral squamous cell carcinoma (OSCC), 28 oral leukoplakia with dysplasia, 10 normal oral mucosa were obtained from Capital Medical University School of Stomatology for immunohistochemical staining of markers of survivin and caspase-3. The cell apoptosis was detected with terminal deoxynucleotidyl transferase-mediated nucleotide shift enzyme (TdT) mediated d-UTP end labeling (TUNEL). Positively stained cells were counted and analyzed statistically to determine potential relationship between survivin, caspase-3 and cell apoptosis., Results: The expression of survivin was faint or negative in normal epithelial cells. The average positive rate of survivin was (1.05 ± 1.21)% in control group and (21.89 ± 10.45)% in OSCC. Caspase-3 was expressed in all the normal mucosa,but it obviously down-regulated in dysplasia and OSCC. The apoptosis index (AI) decreased from (0.89 ± 0.46)% in normal mucosa to (0.21 ± 0.12)% in OSCC., Conclusions: Both survivin and caspase-3 are associated with carcinogenesis of the oral mucosa. Survivin may restrain cell apoptosis by inhibiting caspase-3.

The paper is aimed to establish an artificial neural network (ANN) for predicting mycophenolic acid (MPA) area under the plasma concentration-time curve (AUC) in renal transplantation recipients. 64 Chinese renal transplantation recipients receiving mycophenolate mofetil (MMF) were investigated. 10 timed samples were drawn at different days after transplantation. Plasma MPA concentration was determined by HPLC method and area under curve over the period of 0 to 12 h (AUC(0-12 h)) was calculated using the linear trapezoidal rule. ANN was established after network parameters were optimized using momentum method in combination with genetic algorithm. Furthermore, the predictive performance of ANN was compared with that of multiple linear regression (MLR). When using plasma MPA concentration of 0, 0.5, 2 h after MMF administration to predict MPA AUC(0-12 h), mean prediction error and mean absolute prediction error were -1.53% and 9.12%, respectively. Accuracy and precision of prediction by ANN were superior to that of MLR prediction, and similar results could be found when using plasma MPA concentration of 0, 0.5 h to predict MPA AUC(0-12h). The accuracy and precision of ANN prediction were superior to that of MLR prediction, and ANN can be used to predict MPA AUC(0-12 h).

The study was aimed to investigate the curative effects and adverse effects of amifostine in the treatment of patients with myelodysplastic syndrome (MDS). Amifostine (AMF) was used alone (4/12) or combined with recombinant human erythropoietin (rh-EPO) (8/12) in 12 MDS patients. The therapeutic regimen was adopted with AMF 0.4 g/day for 5 days, then took a break of 2 days and then went on for 3 weeks consecutively, that was reputed as one treatment cycle. rh-EPO 6 000 U was used for 3 days per week. The results showed that 12 patients all attained hematological improvement in peripheral blood. 11 cases showed major effective response rate (91.7%), while 1 case showed minor response rate (8.3%). The effective response rate of hemoglobin, leukocytes and platelets was 100%, 75% and 58.3% respectively. The intervals of red cell transfusions (RCT) in 2 cases living on red cell transfusion before AMF treatment were prolonged after AMF treatments, and the amount of each RCT was decreased obviously. The side effect was usually discomfort of digestive system, but all patients can endure. In conclusion, Amifostine is a potential drug in the treatment of MDS patients with safety especially to those elder patients who often suffered from other multiple organ disfunctions, and the curative effect will be improved by more treatment cycles.

Objective: To study the expression of Ki-67 and the changes of MVD and apoptosis in benign lymphoadenosis of oral mucosa (BLOM)., Methods: The expression of Ki-67, CD34 and apoptosis were evaluated by SP immunohistochemical staining in 15 BLOM, 9 BLOM with dysplasia, 15 oral squamous cell carcinoma (OSCC)., Results: The expression of Ki-67 in BOLM with dysplasia and OSCC was significantly higher than that of BLOM without dysplasia and normal oral mucosa (P < 0.05). The MVD in all BLOM and OSCC was significantly higher than that in normal mucosa (P < 0.05). Apoptosis in BLOM was higher than in normal mucosa and OSCC (P < 0.05)., Conclusions: The expression of Ki-67 and MVD in BLOM with dysplasia were between normal oral mucosa and oral carcinoma. The occurrence of apoptosis in BLOM was significantly higher than in normal oral mucosa. The results suggest that BLOM had the potentiality of malignant transformation.

Three proteins secreted from Mycobacterium tuberculosis, Ag85B, ESAT-6 and MPT63 were selected as antigens for making combined DNA vaccine by immunizing mice. The immune response induced by the vaccine and its protective efficacy were studied. It was demonstrated that when mice were immunized with the combined DNA vaccine, the titer of antibody for Ag85B in serum increased to more than 10(5), but the titers of ESAT-6 and MPT63 specific antibodies were undetectable. After the final immunization, the level of gamma specific for Ag85B, ESAT-6 and MPT63 reached (17.0+/-7.0) u/ml, (6.0+/-0.8) u/ml and (11.9+/-8.0) u/ml, respectively. Mice, that were inoculated with the empty eukaryotic expression vector pJW4303 DNA, produced negligible amounts of antigen-specific INF-gamma. The combined DNA vaccine resulted also in significantly reduced amount of bacteria in the lungs of experimental mice. Microphotographs showed clearly that these lungs were better protected against Mycobacterium tuberculosis challenge than control mice. The combined DNA vaccine reported in this work shed new lights on the prophylactic protection against tuberculosis.