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Objective: To observe the effect of exosomes released by adenoid cystic carcinoma (ACC) cell line SACC-83 on the proliferation of ACC cells., Methods: Exosomes were isolated from SACC-83 cell culture supernatants using total exosome isolation reagents. The whole-mount exosomes were characterized using transmission electron microscope and Western blotting. The exosomes were labeled with green fluorescent dye PKH67 and co-cultured with SACC-83 cells for 48 h, followed by staining with Alexa Fluor 594 phalloidin and DAPI to observe exosome uptake by the cells using laser scanning confocal microscopy (LSCM). The cell proliferation was assessed using MTT assay and wound healing assay, and the expressions of ERK and P-ERK in the co-cultured SACC-83 cells were detected using Western blotting., Results: The exosomes isolated from SACC-83 cells showed a size range of 30-100 nm and expressed the exosomal markers CD9, CD63 and TSG101. LSCM showed exosome uptake by SACC-83 cells, which exhibited accelerated proliferation and significantly enhanced P-ERK expression ( P < 0.05) without significant changes in ERK expression., Conclusions: SACC-83 cells produce exosomes that promote the tumor cell proliferation and enhances the cellular expression of P-ERK, suggesting a potential role of MAPK/ERK pathway activation in exosome-mediated acceleration of ACC cell proliferation.

Objective: To study the relationship between sagittal facet joint and degenerative lumber spondylolisthesis (DLS) by observing the changes of the lumbar facet joint angle., Methods: Fifty-seven patients with DLS who met the inclusion criteria between January 2013 and February 2016 were collected (DLS group). There were 26 males and 31 females, with the mean age of 54.0 years (range, 34-84 years). Forty patients without DLS at same stage were collected as control group. There were 23 males and 17 females with the mean age of 55.6 years (range, 29-82 years). There was no significant difference in gender and age between 2 groups ( P >0.05). The lumbar facet joint angles were measured and compared by MRI scanning images in 2 groups. In DLS group, X-ray films were used to evaluated the degree of the lumbar spondylolisthesis on the basis of the Meyerding standard, and compared the facet joint angles between patients of different DLS degree., Results: Facet joint angles in the DLS group [(34.18± 4.81)°] were significantly smaller than those in control group [(45.87±1.09)°] ( t =15.073, P =0.000). In DLS group, the patients were rated as degree Ⅰ in 24 cases, degree Ⅱ in 19 cases, degree Ⅲ in 14 cases. As the degree of DLS increased, the lumbar joint angle decreased gradually, and showing significantly differences between patients of different DLS degree ( P <0.05)., Conclusion: Sagittal lumbar facet joint may be one of the main risk factors of DLS.

Objective: Lymphoepithelial carcinoma (LEC) of salivary glands is a rare malignant neoplasm. The purpose of this research was to investigate the clinicalpathologic features and treatment methods of this rare disease., Method: The clinical data and treatment outcomes of 17 patients from 2006 to 2012 were reviewed retrospectively., Result: Ten males and seven females with a ratio of 1. 43:1 were involved. The II, III, IV stage cases were 7 (41.2%), 4 (23.5%), 6 (35.3%), respectively. The average follow-up duration was 2.56 years, and 12 patients had no evidence of recurrence. Five patients had local recurrence and (or) distant metastases within three years after surgery, including 4 deaths., Conclusion: LEC in salivary gland is a high grade malignant tumor in oral and maxillofacial region, occurring predominately in parotid gland and submandibular gland. To prevent distant metastasis, radical surgery combined with chemoradiotherapy should be adopted.

Thirteen prevailed Newcastle-disease viruses (NDV) isolated in China during 2001-2004 were purified by chick embryo fibroblast (CEF) plaque assay and characterized pathotypically and genotypically. The biological tests showed that these viruses were highly virulent. Sequence analysis based on the variable region (nucleotide 47-420) of the F gene indicated that of the 13 NDV isolates 2 belonged to genotype II, 2 to genotype IX and 9 to genotype VII. Isolates with genotype VII shared 94.6%-99.3% nucleotide (nt) homology with the F gene, whereas for genotype VII and La Sota was only 82.7%-84.1%. In addition, these NDV isolates all shared 95.2%-100% nt homology with the hemagglutinin-neuraminidase (HN) gene, whereas only 79.1%-84.3% compared these viruses with La Sota. The cross neutralization assays were done using positive serums in specific pathogen free (SPF) chicken embryos respectively. Correlation of the neutralization index in chicken embryo with the homologies of F and HN gene of different NDV isolates were analyzed by SPSS8.0 software. The result showed that the neutralization index was closely correlated with nt sequence (P < 0.01, r = 0.35) or deduced amino acid sequence (P < 0.01, r = 0.34) of the HN gene, whereas weekly correlated (P < 0.05, r = 0.20 or 0.19) with the F gene, and non-correlated with 374 nt segment. This implied that the genetic mutations of HN resulted in antigenic variations of these viruses and the search for new vaccines would be necessary.