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To optimize the processing technology of Gastrodia elata by water in which millet had been cooked and investigate the effects of LPS-induced microglia (BV2) inflammatory responses. Determination of gastrodin, p-hydroxybenzyl alcohol, parishin A, parishin B, parishin C, parishin E in the extract of Gastrodia elata by high-performance liquid chromatography (HPLC), and the content of six components were used as evaluation index, the volume ratio of water in which millet had been cooked to water, cooking time and drying temperature were selected as single factor variables. The designation of orthogonal test was based on single factor on three indicators, then optimization of the preparation technology. Comparison of the content of six components in different processed product. The neuroinflammatory cell model was induced by LPS and the cell viability was evaluated after treatment with the extract of processed products by a Cell Counting kit 8 (CCK-8) assay. The levels of TNF-α, IL-6, IL-1β in the cell culture medium were measured with ELISA kits. The protein expressions of TLR4, MYD88, P65, INOS, COX-2, IL-1β in LPS-induced BV2 cells were detected by Western blot. The results showed that the cooking time and the volume ratio of water in which millet had been cooked to water had a significant effect on the test results. The water in which millet had been cooked of Gastrodia elata processing technology: The ratio of the volume water in which millet had been cooked to water was 1:3, the boiling time was 25 min and the temperature of drying was 75 ℃. The comprehensive score of the six components in processing with water in which millet had been cooked products was higher than that of steamed products. The extract of Gastrodia elata had exhibited varying degrees of protective activity on LPS-induced BV2 cells. The results showed that the secretion of TNF-α, IL-6, IL-1β was significantly increased in BV2 microglial cells induced by LPS compared with the normal control group (P

The accuracy of the Sliding grid calculation method applied to the aerodynamics calculation is verified with an example in hovering, then, the aerodynamic parameters of cyclorotor with different radius, chord and number of blades are studied, and the aerodynamic interfere of cyclorotor with different distances is calculated and analyzed. The results show that with the increase of radius, the aerodynamics and the load per unit area of the blade increase; the larger the chord, the larger the aerodynamics, the smaller the load per unit area of the blade; the aerodynamics generated by the 4-blade cyclorotor is larger than that of 3-blade cyclorotor and 6-blade cyclorotor, while the blade load of 3-blade cyclorotor is the largest; the resultant deflection angle decreases with the increase of the rotational speed and the solidity respectively; with the increase of the distance among the cyclorotors, the aerodynamic loss coefficient and the resultant force deflection angle are decreased.

According to the reform idea of "One center, two integrations and three dependences", the experimental teaching system of the Optoelectronic Information Science and Engineering specialty is reformed by taking students' engineering practice ability as the center, integrating science and education with production and teaching, relying on the experimental course construction team, enterprise technology team and laboratory management team, the "Four modernizations" reform of experimental teaching system of Optoelectronic Information Science and Engineering specialty is carried out, that is, the modularization of experimental curriculum system, construction and development of the experimental platform, classification of experimental courses and engineering of frontier experimental project. The reform has achieved a number of reform results and has improved the guarantee of professional experimental teaching team. [ABSTRACT FROM AUTHOR]

Objective To investigate the effects of NDUFA4 overexpression on the growth of human colon cancer cells in vitro and to explore its potential mechanism. Methods The recombinant plasmid p-NDUFA4 (p-NDUFA4) and the control plasmid (p-Cont) were transiently transfected into human colon cancer HCT 116 cells, respectively. Then, the relative expression level of NDUFA4 was determined by Real-time PCR and Western blot. The proliferation and colony formation ability of HCT116 cells were detected by CCK-8 assay and clone formation assay. The mRNA expression levels of cyclin-dependent kinase (CDK) 2, CDK3, CDK4 and CDK6 were determined by Real-time PCR. The expression levels of AKT (p-AKT) and ERK1/2(p-ERK1/2) were analyzed by Western blot. Results Compared with control group, the expression levels of NDUFA4 were increased remarkably in p-NDUFA4 group (all P < 0.01). The proliferation and clone formation capacity of HCT116 cells in p-NDUFA4 group were obviously increased (all P < 0.05). The expression of CDK2 and CDK3 mRNA were increased significantly(all P < 0.05). The expression of p-AKT and p-ERK1/2 were increased obviously (all P < 0.05). Conclusion The overexpression of NDUFA4 could significantly promote the growth of human colon cancer HCT116 cells in vitro, which might be closely related to the change of AKT and ERK signaling transduction.

Aiming at the urgent demand of unmanned and intelligent border security system, an unattended border monitoring system is designed for weak infrastructure border area. The system composition and sub-system design are introduced, and the key technologies for engineering realization are given, ground surveillance radar and electro-optics device single-site collaborative detection and tracking technology, and multi-target tracking algorithm with intermittent observations are proposed. The realization and demonstration verification test of prototype system prove the scientificity and rationality of the design, which achieve the tactical and technical requirements of high point monitoring with the unattended capability.

At present,there are few studies focusing on the effect of negative bias voltage on the corrosion resistance of TiAlN films deposited by arc ion plating.In this paper,TiAlN films were deposited on M2 high speed steel by arc ion plating,and scanning electron microscope (SEM),X-ray diffraction (XRD),electrochemical test and other methods were used to study the influences of negative bias voltage on substrate on the microstructure,surface morphology and corrosion resistance of the as-prepared films.Results showed that the negative bias voltage was an important process parameter affecting the surface morphology of TiAlN films deposited by arc ion plating.A proper negative bias voltage could effectively improve the morphology and compactness of the surface film and reduce the size and number of large molten droplets on the surface.The main phase of TiAlN films was AlTi3N(111),and the films mainly grew along the (111) direction.When the negative bias voltage was increased,a new reaction would occur and a Ti2AlN (100) diffraction peak would appear.With the increase of the negative bias voltage on substrate,the microhardness of the films increased first and then decreased.When the negative bias voltage was 150 V,the microhardness reached the maximum value of 2 725 HV,the relative corrosion rate was the lowest,and the corrosion resistance was the best.

Objective: To investigate mechanism underlying the role of nuclear factor Kappa B (NF-κB) which induced inflammatory injury and functional lesions of aortic endothelial cells in rat with emphysema and intermittent hypoxia., Methods: Sixty male Wistar rats were divided randomly into 4 experimental groups (n = 15 each group): control group, emphysema group, intermittent hypoxia (IH) group, emphysema with intermittent hypoxia group. The rats in control group had ad libitum access to food and water under normal circumstance. The rats in the emphysema group were exposed to cigarette smoke twice daily (30 min each time). As for IH group, the rats were exposed to intermittent hypoxia circumstance (8 h/day). Both cigarette smoke twice a day (30 min each time) and intermittent hypoxia circumstance (8 h/day) were imposed on the rats in emphysema with intermittent hypoxia group. All the rats were exposed for 8 weeks. Five rats were randomly selected from each group to measure the blood gas on the ninth week. We collected lung and endothelial tissues of thoracic aorta from the rest sacrificed rats, and observed the pathological changes of lung tissue through HE staining. The levels of ET-1, TNF-α and IL-8 in rat endothelial tissues of thoracic aorta were measured by ELISA testing. Nitrate reductase was used to measure the levels of NO, and RT-PCR to detect the levels of NF-κB mRNA, ICAM-1 mRNA, MMP-9 mRNA and eNOS mRNA., Results: Lung pathology and blood gas results showed that the rat model of emphysema with intermittent hypoxia was established successfully. The levels of ET-1, TNF-α, IL-8 in emphysema with intermittent hypoxia group were (172.4 ± 1.6) ng/L, (104.1 ± 1.4) ng/L, (272.1 ± 3.6) ng/L respectively, significantly higher than the control group, emphysema group and intermittent hypoxia group (all P < 0.05). The level of NO was (27.07 ± 0.57) µmol/L, which was significant reduced; the expression of NF-κB mRNA, ICAM-1 mRNA, MMP-9 mRNA in emphysema with intermittent hypoxia group was significantly upregulated compared with the control goup, emphysema group and intermittent hypoxia group (all P < 0.05). The levels of eNOS mRNA expression were significantly lower than other three groups. The expression of NF-κB mRNA was positively correlated with MMP-9 mRNA level (r = 0.572, P < 0.001) and the expression of NF-κB mRNA was negatively correlated with eNOS mRNA level (r = 0.534, P < 0.001); there was no statistical difference in levels of NF-κB mRNA and eNOS mRNA expression between intermittent hypoxia and emphysema group (P > 0.05)., Conclusion: Compared with only emphysema or intermittent hypoxia exposure, inflammatory injury of aortic endothelial cells of rats induced by emphysema with intermittent hypoxia was more serious, and may result in more serious cardiovascular complications. The activation of NF-κB pathway may be an important mechanism of its inflammatory response.

Infection with the hepatitis C virus (HCV) frequently causes chronic viral hepatitis, a major risk factor for the development of cirrhosis and hepatocellular carcinoma or primary liver cancer. The origin of the HCV remains obscure because no closely related animal virus homolog has been identified. Also, efforts to understand the pathogenesis of the HCV have been hampered by the absence of small animal models for this human disease. Since 2011, with application of new sequencing technologies, various non-primate HCV homologs have been identified that will play an important part in understanding the origin and evolution of HCV, as well as establishment of related animal models.

Objective: To investigate genetic polymorphisms of 12 X chromosome short tandem repeat (X-STR) loci in ethnic Hebei Han population using an Investigator Argus X-12 amplification kit., Methods: DNA was extracted for 198 unrelated individuals (96 males and 102 females) and amplified with a fluorescence labeled multiplex PCR system. PCR products were separated and genotyped with capillary array electrophoresis., Results: Only DXS10103 and DXS10101 showed significant linkage disequilibrium at the 12 X-STR loci. One hundred and forty-eight alleles, including 22 off-ladder (OL) alleles, were observed at the 12 X-STR loci in the population. The heterozygosity and polymorphic information content (PIC) were 0.5074-0.9143 and 0.4377-0.9079, respectively. The power of discrimination (PD) was 0.5074-0.9143 in males and 0.6876-0.9863 in females. The mean exclusion chance was 0.4377-0.9079 in the trios cases and 0.2984-0.8373 in the duo cases, respectively., Conclusion: The Investigator Argus X12 amplification system is highly polymorphic in ethnic Han population from Hebei and is useful for personal identification and paternity testing.

Lipid droplets are the main storage organelles for intracellular neutral lipids. Many recent studies have found that lipid droplets are closely related to hepatitis C virus (HCV). Lipid droplets play important roles in the multiple processes of HCV life cycle, such as infection, replication, assembly, and secretion. In this review, we summarize the recent research progress in the roles of lipid droplets in HCV life cycle.

COPI is a protein complex that transports vesicles from the Golgi complex back to endoplasmic reticulum. Many viruses such as RNA viruses, DNA viruses and retroviruses, hijack or adapt COPI related proteins including coatomer, ARF1 and GBF1 for their own benefits. Here, we summarize the current progress of the roles of COPI related proteins in virus replication.