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To study the secondary metabolites of a marine-derived fungus Ascotricha sp. ZJ-M-5, several chromatographic methods including macroporous resin, silica gel, ODS and Sephadex LH-20 were used to isolate the compounds, and their structures were elucidated on the basis of physicochemical properties and spectroscopic methods. Ten compounds were obtained and identified as ascotrichic acid B (1), (3R)-6-hydroxymellein (2), beta-carboline (3), (22E, 24R)-ergosta-7, 22-diene-3beta, 5alpha, 6beta-triol (4), (22E, 24R)-ergosta-7, 22-diene-3beta, 5alpha, 6beta, 9alpha-tetraol (5), cyclo (Leu-Pro) (6), cyclo (Ile-Leu) (7), cyclo (Pro-Val) (8), cyclo (Pro-Gly) (9), and cyclo (Hpro-Ala) (10). Among them, compound 1 is a new 3, 4-seco-lanostane triterpenoid which has been isolated from the filamentous fungi for the first time, and compounds 2-10 are firstly isolated from Ascotricha genus.

To investigate the effects of lanosterol (1), inotodiol (2) and trametenolic acid (3) from Inonotus obliquus against oxidative damage induced by CCl4 in mice, 1, 2 and 3 (20, 10 and 5 mg x kg(-1)) were respectively administered to mice, once a day for 3 days. Then the mice were induced to oxidative damage by CCl4 on the third day 30 min after the administration. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-PX) and the content of malondialdehyde (MDA) and reductive glutathione (GSH) in serum and liver homogenate were determined. And the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and interleukin-6 (IL-6) concentration in serum were detected. The results showed that treatment with compound 1, 2 and 3 could significantly increase the activities of SOD, CAT and GSH-PX in serum and liver homogenate. Furthermore, the content of GSH in serum and liver homogenate increased and MDA content decreased markedly. In addition, compound 1, 2 and 3 could significantly inhibit the activities of ALT and AST in serum, and decrease the IL-6 concentration in serum remarkably. So, compound 1, 2 and 3 can protect mice against oxidative stress injury induced by CCl4. Furthermore, compound 1, 2 and 3 can protect cells from damage through inhibition on ALT, AST and the expression of IL-6.

Objective: To study the secondary metabolites of Coriolopsis sp. G066., Methods: The compounds were isolated by various chromatographic methods (silica gel, reverse silica gel, Sephadex LH-20, preparative TLC and so on). Their structures were determined by extensive analysis of their spectroscopic data as well as by comparison with literature reports., Results: Six compounds were isolated and identified as diaporthein A(1), tyrosol(2), eburicoic acid(3), ergosterol(4), ergosterol peroxide(5) and cerevisterol (6)., Conclusion: All the compounds are isolated from the genus for the first time.

Objective: To study the chemical constituent from the antithrombus activity extract of Radix Aerio Fici Microcarpae., Methods: The isolation and purification of the extract were conducted by means of silica gel column chromatography, polyamide column chromatography and recrytallization. The structures of these compounds were elucidated based on the physicochemical properties and spectroscopic analysis., Results: 7 compounds were isolated from the extract. They were identified as isovanillic acid (1), 4-hydroxybenzoic acid(2),3,4-dihydroxybenzoic acid (3), friedelane (4), alpha-amyrin acetate (5), lanosterol (6) and beta-sitosterol (7) ., Conclusion: Compound 3,4 and 7 are isolated from this plant for the first time.

Objective: To study the chemical constituents of the sporoderm-broken spores of Ganoderma lucidum., Methods: Chemical constituents were isolated and purified by silica gel and Sephadex LH-20 column chromatography. The structures were identified by means of physicochemical and spectral data., Results: From the ethyl acetate extract of the material, eight compounds were isolated. Their structures were identified as ganoderic acid A (I), methyl ganoderate A (II), methyl ganoderate B (III), ganoderic acid C2 (IV), ganoderic acid G(V), ergosta-7,22-diene-3beta, 5alpha, 6beta-triol (VI), ergosterol peroxide (VII) and ergosta-7,22-diene-3beta-yl pentadecanoate (VIII), respectively., Conclusion: Compounds II, III, VII and VIII are isolated from the spores of G. lucidum for the first time.

Objective: To investigate the chemical constituents of the rhizomes of Actaea asiatica in order to obtain a more comprehensive understanding of its effective components., Method: Compounds were separated by silica gel chromatography, RP-C18 chromatography and semi-preparative high performance liquid chromatography, and their structures were established by spectral analysis and chemical evidence., Result: Six compounds were isolated from the ethyl acetate extract. Their structures were identified as 25-O-acetylcimigenol (1), 12beta-hydroxycimigenol (2), 23-epi-26-deoxyactein (3), 27-deoxyacetylacteol (4), 26-deoxycimicifugenin (5) and beta-sitosterol (6)., Conclusion: All these compounds mentioned above were isolated from the plant for the first time.

Objective: To study the chemical constituents in the whole herb of Anoectochiluts roxburghii (Wall) Lindl., Method: The chemical constituents were isolated by various column chromatographic methods and structurally elucidated by IR, NMR and MS evidences., Result: Fourteen compounds were obtained and there into ten were identified as beta-sitosterol(2), ferulic acid(3), oleanolic acid(6), lanosterol(7), p-hydroxybenzaldehyde(8), 3-methoxyl-p-hydroxybenzaldehlde(9), daucosterol(10),3',4',7-trimethoxy-3, 5-dihydroxyflavone(11), isorhamnetin-3-O-beta-D-rutinoside(12) and rutin(13)., Conclusion: Compounds 6,7,9 and 11 from genera Anoeetochilus were isolated for the first time.

Objective: To study the constituents of the roots of Euphorbia hylonoma Hand. -Mazz., Methods: The chemical constituents were isolated and purified by solvent extraction together with various chromatographic techniques, the structures were elucidated on the basis of chemical properties and spectral data., Results: Eight compounds were isolated from the acetone extracts of the roots of Euphorbia hylonoma Hand-Mazz., which were euphol(I), 5-hydroxymethylfuraldehyde(II), beta-sitosterol(III), beta-sitosterol glucoside (IV),3,3',4-tri-o-methylellagic acid(V), 3, 3' tdi-o-methylellagic acid (VI),3,3'-di-o-methylellagic acid-4'-o-beta-dxylopyranosid (VII), gallic acid(VIII)., Conclusion: The compounds II, VI, VII, VIII were isolated from Euphorbia hylonoma Hand-Mazz. for the first time.

Objective: To study the constituents from roots of Euphorbia hylonoma., Method: Column chromatographic techniques were used for isolation and purification of the chemical constituents and their structures were identified by spectral analysis (IR, 1H-NMR, 13C-NMR, 2D-NMR and MS)., Result: Six compounds were isolated and elucidated as nonane (1), bis (2-ethylhexyl) phthalate (2), euphol (3), beta-sitosterol (4), acalyphol (5) and daucosterol (6) respectively., Conclusion: Compounds 1, 2, 3, 5 and 6 were isolated from the plant for the first time.

Objective: To study the chemical constituents of Chaetomorpha basiretorsa and screen for bioactive leading compounds., Method: Compounds were isolated by normal phase silica gel and Sephadex LH-20 gel column chromatography, reverse phase MPLC and reverse phase HPLC. Their structures were elucidated by spectroscopic methods including MS, IR and 1D, 2D NMR. Cytotoxicity of the compounds was screened by using standard MTT method. The purified compounds' inhibition against proliferation of dog vascular smooth muscle cells was also screened by MTT assay., Result: Five compounds were isolated from C. basiretorsa and their structures were identified as euphol (I), loloilide (II), 4-cumylphenol (III), zeaxanthin (IV) and lactucaxanthin (V)., Conclusion: All these compounds were obtained from this genus for the first time. Compound (III), 4-cumylphenol, was a new nature product. All compounds were inactive (IC50 > 10 microg x mL(-1)) in cytotoxicity screening. In inhibition against proliferation of dog vascular smooth muscle cells test, the cell survival ratio to compound I was (0.32 +/- 0.056)% which indicate its potential anti-atherosclerotic bioactivity.

Objective: To find new active constituents from the aerial part of Cimicifuga foetida., Method: Various column chromatographic techniques were used for the isolation and purification of the principles. The structures were elucidated on the basis of spectral data and chemical evidences., Result: Four 9,19-cycloartane triterpenoid saponins were obtained and identified as Cimifoetiside III (25-anhydrocimigenol-3-O-beta-D-galactopyranoside, 1), 25-O-acetyl-cimigenol xylopyranoside (2), 25-O-acetyl-cimigenol galactopyranoside (3), 7 beta-hydrocimigenol xylopyranoside (4)., Conclusion: Compound 1 is new and compound 4 was isolated from this plant for the first time.

Objective: To find new active constituents from Rhizome of Cimicifuga foetida., Method: Various column chromatographic techniques were employed for isolation and purification. The structures were elucidated on the basis of spectral and chemical evidences., Result: Four triterpenoid compounds were isolated and identified as 7,8-didehydro-27-deoxyactein(1), 24-O-acetylshengmanol-3-O-beta-D-xyl (23R, 24R)[2], cimigenol(3), cimigenol-3-O-beta-D-xyl(4)., Conclusion: Compound 1 is a new compound, 2-4 were obtained from this medicinal material for the first time. The antiosteoporosis activity screening in vitro(by the method of SRB) indicates that Compounds 1, 2 and 4 can promote the proliferation for rat Osteoblastoma cell line (UMR106) at the concentration of 10(-9) kg.L-1.

Aim: To study the chemical constituents of the fruiting bodies of Ganoderma lucidum., Methods: Individual constituents, isolated and repeatedly purified on silica gel column, were identified by physicochemical constants and structurally elucidated by spectral methods., Results: From the alcohol extract, compound 2 was obtained and identified as 3 beta,7 beta-dihydroxy-4,4,14 alpha-trimethyl-11,15-dioxo-5 alpha-chol-8-en-24-oic acid. In addition, two known compounds, lucidenic acid A (1) and C (3) were obtained., Conclusion: Compound 2 is a new triterpene compound.

Three compounds have been isolated from the dichloromethane soluble fraction of the fruiting body of Ganoderma lucidum (Fr.) Karst. On basis of spectral analyses (UV, IR, MS, 1HNMR, 13CNMR and 2D-NMR), they were identified as 3, 7-dioxo-lanosta-8, 24(E)-dien-26-oic acid (I), 7 beta-15 alpha-dihydroxy-3, 11, 23-trioxo-5 alpha-lanost-8-en-26-oic acid (II) and 3 beta, 7 beta, 15 alpha-trihydroxy-11, 23-dioxo-5 alpha-lanosta-8-en-26-oic acid (III). Compound I is a new compound named ganoderic acid DM.

Crystals I-N were isolated from the ethereal extracts of Poria cocos and identified respectively as O-acetyl-pachymic acid (I), 3 beta-hydroxy-lanosta-7,9(11),24-trien-21-oic acid (II), beta-amyrin acetate (III) and 3 beta-hydroxy-16 alpha-acetoxy-lanosta-7,9(11),24-trien-21-oic acid (N). Crystal III was obtained from P. cocos for the first time, and crystal N was newly discovered.

Five compounds were isolated from the ether soluble fraction of the spores of Ganoderma lucidum. On the basis of their chemical properties and spectral data (MS, UV, IR, 1H and 13CNMR), they were identified as 3,7,11,12,15,23-hexaoxo-5 alpha-lanosta-8-en-26-oic acid (I), 3 beta,7 beta-dihydroxy-11,15,23-trioxo-5 alpha-lanosta-8-en-26-oic acid (II), 7 beta-hydroxy-3,11,15,23-tetraoxo-5 alpha-lanosta-8-en-26-oic acid (III), 3,7,11,15,23-pentaoxo-5 alpha-lanosta-8-en-26-oic acid (IV), 24,25,26-trihydroxy-5 alpha-lanosta-7,9 (11)-dien-3-one (V), Compound I is a new natural product, named ganosporeric acid A. Compounds II, III, IV and V were obtained for the first time from the spores of Ganoderma lucidum.